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Bacterial Plasma Membrane Dynamics

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: closed (30 September 2020) | Viewed by 5072

Special Issue Editor


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Guest Editor
Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan
Interests: microbial evolution; cell enlargement; microinjection of long DNA into bacterial cell; cell-cell communication; genome evolution; microbioinformatics
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Special Issue Information

Dear colleagues,

The cell cycle mechanism of bacteria has not been studied as much as that of eukaryotic cells. For example, little research has been done on the relationship between DNA replication and plasma membrane biosynthesis. Studies on enlargement of bacterial protoplasts or spheroplasts showed that plasma membrane and outer membrane biosyntheses require metal ions and DNA replication together with generating vacuoles in the cytoplasm. How do those bacterial protoplasts or spheroplasts maintain the electron transport chain system during enlargement of the plasma membrane? This research topic focuses on the bacterial plasma membrane dynamics, which includes its evolution, function, and strucutre.

Dr. Hiromi Nishida
Guest Editor

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Keywords

  • bacterial cell surface
  • S-layer
  • outer membrane
  • plasma membrane
  • DNA replication
  • cell enlargement
  • cell division
  • vacuole formation

Published Papers (1 paper)

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Review

11 pages, 906 KiB  
Review
Factors That Affect the Enlargement of Bacterial Protoplasts and Spheroplasts
by Hiromi Nishida
Int. J. Mol. Sci. 2020, 21(19), 7131; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21197131 - 27 Sep 2020
Cited by 7 | Viewed by 4499
Abstract
Cell enlargement is essential for the microinjection of various substances into bacterial cells. The cell wall (peptidoglycan) inhibits cell enlargement. Thus, bacterial protoplasts/spheroplasts are used for enlargement because they lack cell wall. Though bacterial species that are capable of gene manipulation are limited, [...] Read more.
Cell enlargement is essential for the microinjection of various substances into bacterial cells. The cell wall (peptidoglycan) inhibits cell enlargement. Thus, bacterial protoplasts/spheroplasts are used for enlargement because they lack cell wall. Though bacterial species that are capable of gene manipulation are limited, procedure for bacterial cell enlargement does not involve any gene manipulation technique. In order to prevent cell wall resynthesis during enlargement of protoplasts/spheroplasts, incubation media are supplemented with inhibitors of peptidoglycan biosynthesis such as penicillin. Moreover, metal ion composition in the incubation medium affects the properties of the plasma membrane. Therefore, in order to generate enlarged cells that are suitable for microinjection, metal ion composition in the medium should be considered. Experiment of bacterial protoplast or spheroplast enlargement is useful for studies on bacterial plasma membrane biosynthesis. In this paper, we have summarized the factors that influence bacterial cell enlargement. Full article
(This article belongs to the Special Issue Bacterial Plasma Membrane Dynamics)
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