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Special Issue "Biofabrication for Tissue Engineering Applications"

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Materials Science".

Deadline for manuscript submissions: 30 April 2022.

Special Issue Editor

Dr. Emmanuel Stratakis
E-Mail Website
Guest Editor
Foundation for Research and Technology-Hellas (F.O.R.T.H.), Institute of Electronic Structure and Laser (I.E.S.L.), 70013 Heraklion, Greece
Interests: biosensors; bioelectronics; biomaterials; tissue engineering; biosurfaces; biointerfaces
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The concept of regenerating tissues, with properties and functions that mimic natural tissues, has attracted significant attention in recent years, as it provides potential solutions for many disease treatments and demanding healthcare problems. To fully realize the potential of the approach, it is crucial to have a rational biomaterial design and subsequent fabrication to create novel scaffolds and material systems and devices suitable for tissue engineering, repair, and regeneration. As a consequence of the intense research activity in the field, a variety of 3D and 4D biofabrication approaches has been developed, including soft lithography and self-assembly, as well as subtractive (top-down), additive (bottom-up) and hybrid manufacturing. Further research advances in the topic include the design of new and smart biomaterials, the fabrication of implantable multifunctional scaffolds and devices for disease monitoring, diagnostics and treatment, as well as the manufacturing of artificial tissues and organs.

This Special Issue on “Biofabrication for Tissue Engineering” welcomes original research and review articles in this rapidly growing field that are related to the development of biomaterial scaffolds, biomedical devices and organ-on-a-chip systems for tissue engineering applications. It will focus on all aspects of biofabrication, including design, manufacturing, functionalization, characterization, evaluation of novel scaffolds, biomedical devices and systems for tissue engineering and regeneration, aiming at disease diagnoses and treatments.

Dr. Emmanuel Stratakis
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biofabrication
  • biomaterials processing
  • tissue engineering systems
  • advanced bioimaging

Published Papers (2 papers)

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Research

Article
A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction
Int. J. Mol. Sci. 2021, 22(18), 10067; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221810067 - 17 Sep 2021
Viewed by 627
Abstract
The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted. [...] Read more.
The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted. A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure. Therefore, we evaluated the decellularized human limbus (DHL) as a biomimetic scaffold for the transplantation of LEPCs. Corneoscleral tissue was decellularized by sodium deoxycholate and deoxyribonuclease I in the presence or absence of dextran. We evaluated the efficiency of decellularization and its effects on the ultrastructure and ECM composition of the human corneal limbus. The recellularization of these scaffolds was studied by plating cultured LEPCs and limbal melanocytes (LMs) or by allowing cells to migrate from the host tissue following a lamellar transplantation ex vivo. Our decellularization protocol rapidly and effectively removed cellular and nuclear material while preserving the native ECM composition. In vitro recellularization by LEPCs and LMs demonstrated the good biocompatibility of the DHL and intrastromal invasion of LEPCs. Ex vivo transplantation of DHL revealed complete epithelialization as well as melanocytic and stromal repopulation from the host tissue. Thus, the generated DHL scaffold could be a promising biological material as a carrier for the transplantation of LEPCs to treat LSCD. Full article
(This article belongs to the Special Issue Biofabrication for Tissue Engineering Applications)
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Article
FAK Shutdown: Consequences on Epithelial Morphogenesis and Biomarker Expression Involving an Innovative Biomaterial for Tissue Regeneration
Int. J. Mol. Sci. 2021, 22(18), 9774; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22189774 - 10 Sep 2021
Viewed by 453
Abstract
By employing an innovative biohybrid membrane, the present study aimed at elucidating the mechanistic role of the focal adhesion kinase (FAK) in epithelial morphogenesis in vitro over 4, 7, and 10 days. The consequences of siRNA-mediated FAK knockdown on epithelial morphogenesis were monitored [...] Read more.
By employing an innovative biohybrid membrane, the present study aimed at elucidating the mechanistic role of the focal adhesion kinase (FAK) in epithelial morphogenesis in vitro over 4, 7, and 10 days. The consequences of siRNA-mediated FAK knockdown on epithelial morphogenesis were monitored by quantifying cell layers and detecting the expression of biomarkers of epithelial differentiation and homeostasis. Histologic examination of FAK-depleted samples showed a significant increase in cell layers resembling epithelial hyperplasia. Semiquantitative fluorescence imaging (SQFI) revealed tissue homeostatic disturbances by significantly increased involucrin expression over time, persistence of yes-associated protein (YAP) and an increase of keratin (K) 1 at day 4. The dysbalanced involucrin pattern was underscored by ROCK-IISer1366 activity at day 7 and 10. SQFI data were confirmed by quantitative PCR and Western blot analysis, thereby corroborating the FAK shutdown-related expression changes. The artificial FAK shutdown was also associated with a significantly higher expression of filaggrin at day 10, sustained keratinocyte proliferation, and the dysregulated expression of K19 and vimentin. These siRNA-induced consequences indicate the mechanistic role of FAK in epithelial morphogenesis by simultaneously considering prospective biomaterial-based epithelial regenerative approaches. Full article
(This article belongs to the Special Issue Biofabrication for Tissue Engineering Applications)
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