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Special Issue "Biotransformation of Natural Products and Phytochemicals: Metabolites, Their Preparation and Properties"

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Bioactives and Nutraceuticals".

Deadline for manuscript submissions: 30 September 2021.

Special Issue Editors

Dr. Kateřina Valentová
E-Mail Website
Guest Editor
Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Vídeňská 1083, CZ 142 20 Praha 4, Czech Republic
Interests: polyphenols; flavonolignans; Silybum marianum; flavonols; quercetin; rutin; isoquercitrin; metabolites; gut microbiota; biotransformation by enzymes and whole cells
Special Issues and Collections in MDPI journals
Prof. Dr. Vladimír Křen
E-Mail Website
Guest Editor
Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, National Centre of Biocatalysis and Biotransformation, Videnska 1083, CZ 142 20 Praha 4, Czech Republic
Interests: biocatalysis and biotransformation; immobilized microbial cells, their use in production and biotransformation of natural products; biotransformation of natural products by enzymes and microorganisms; preparation of glycosidases of microbial origin and their use for glycosylation of natural compounds: glycoconjugates, multivalent compounds, ergot alkaloids, flavonoids, antioxidants and chemoprotectants
Special Issues and Collections in MDPI journals

Special Issue Information

Dear Colleagues,

Biotransformation features two major meanings: (i) biocatalysis and enzymatic modification of various compounds and (ii) biotransformation in terms of metabolic changes of xenobiotics in organism. In this special issue we want to focus on both connotations of biotransformation; specifically to the biotransformation of natural products and phytochemicals. Metabolites of xenobiotics have often different biological and biophysical properties compared to the parent compounds. Besides inactivation, also toxication and/or alteration of their activities often happen. Phytochemicals and other natural products have received increased attention due to their considerable benefits in preventing and managing chronic diseases. These compounds generally suffer from low bioavailability, they are extensively metabolized and their metabolites are often responsible for their biological effects. Therefore preparation and detailed study of their metabolites is of utmost importance. Due to the complexity of phytochemicals enzymatic methods were recently introduced in the preparation of their (tentative) metabolites. Topics for this Special Issue include the discovery of new chemical and biochemical methods for metabolite preparation including multienzyme methods, advanced structural and analytical methods for their characterization, and determination of biological and biophysical properties of the metabolites.

Major requirements for papers submitted to this Special Issue are (i) clear novelty; (ii) reproducibility; (iii) molecular bases of reactions and processes; and (iv) defined chemical reactions and structures. Papers dealing with minor optimizations of known procedures, those using poorly defined substrates and products and poorly defined biocatalysts (enzymes) or organisms unavailable to other researchers will be returned without further review.

Dr. Kateřina Valentová
Prof. Dr. Vladimír Křen
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • natural product biotransformation
  • phytochemical metabolism
  • preparation of metabolites
  • cofactor regeneration
  • multienzyme processes
  • metabolite analysis and characterization
  • toxicology of the metabolites

Published Papers (1 paper)

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Research

Open AccessArticle
Bioconversion of Callus-Produced Precursors to Silymarin Derivatives in Silybum marianum Leaves for the Production of Bioactive Compounds
Int. J. Mol. Sci. 2021, 22(4), 2149; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22042149 - 21 Feb 2021
Viewed by 513
Abstract
The present study aimed to investigate the enzymatic potential of Silybum marianum leaves to bioconvert phenolic acids produced in S. marianum callus into silymarin derivatives as chemopreventive agent. Here we demonstrate that despite the fact that leaves of S. marianum did not accumulate [...] Read more.
The present study aimed to investigate the enzymatic potential of Silybum marianum leaves to bioconvert phenolic acids produced in S. marianum callus into silymarin derivatives as chemopreventive agent. Here we demonstrate that despite the fact that leaves of S. marianum did not accumulate silymarin themselves, expanding leaves had the full capacity to convert di-caffeoylquinic acid to silymarin complex. This was proven by HPLC separations coupled with electrospray ionization mass spectrometry (ESI-MS) analysis. Soaking the leaf discs with S. marianum callus extract for different times revealed that silymarin derivatives had been formed at high yield after 16 h. Bioconverted products displayed the same retention time and the same mass spectra (MS or MS/MS) as standard silymarin. Bioconversion was achieved only when using leaves of a specific age, as both very young and old leaves failed to produce silymarin from callus extract. Only medium leaves had the metabolic capacity to convert callus components into silymarin. The results revealed higher activities of enzymes of the phenylpropanoid pathway in medium leaves than in young and old leaves. It is concluded that cotyledon-derived callus efficiently produces compounds that can be bio-converted to flavonolignans in leaves tissue of S. marianum. Full article
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