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Neuro-Centric Applications of Zebrafish: Models and Risk Factors

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Genetics and Genomics".

Deadline for manuscript submissions: closed (31 May 2022) | Viewed by 2634

Special Issue Editor


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Guest Editor
Korea Research Institute of Bioscience and Biotechnology, Bioresource Building 2025, Gwahak-ro 125, Yuseong-gu, Daejeon, Korea
Interests: zebrafish; neuro-centric; neurodevelopmental; neurophysiological; neuropathological; neurodegenerative diseases

Special Issue Information

Dear Colleagues,

Since introduced as a genetic animal model, zebrafish has been successfully employed to understand a plethora of neurodevelopmental, neurophysiological, neuropathological processes due to its unique advantages including its transparency of the nervous system, amenable genetics, live imaging, and behavioral paradigms. Thus, zebrafish becomes an indispensable vertebrate model for human neuro-related diseases and mechanical studies, and provides a valuable in vivo platform to assess risk factors that affect neuro-function/pathology. This special issue aims to cover the most up-to-date zebrafish models to study neurodevelopmental disorders (e.g., autism spectrum disorder, attention deficit hyperactivity disorder) as well as neurodegenerative diseases (e.g., Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, peripheral neuropathy). In addition, this issue will also cover the contribution of non-neural and environmental risk factors, such as microbiome, infection, metabolism, and chemicals/nanomaterials, to neuro-related physiology and pathological conditions using zebrafish.

Dr. Jeong-Soo Lee
Guest Editor

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Keywords

  • zebrafish
  • neuro-centric
  • neurodevelopmental
  • neurophysiological
  • neuropathological
  • neurodegenerative diseases
  • risk factors
  • microbiome
  • infection
  • chemicals
  • nanomaterials
  • metabolism

Published Papers (1 paper)

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Research

16 pages, 2625 KiB  
Article
Tryptophan Hydroxylase 2 Deficiency Modifies the Effects of Fluoxetine and Pargyline on the Behavior, 5-HT- and BDNF-Systems in the Brain of Zebrafish (Danio rerio)
by Valentina S. Evsiukova, Daria Bazovkina, Ekaterina Bazhenova, Elizabeth A. Kulikova and Alexander V. Kulikov
Int. J. Mol. Sci. 2021, 22(23), 12851; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222312851 - 27 Nov 2021
Cited by 9 | Viewed by 2091
Abstract
The mechanisms of resistance to antidepressant drugs is a key and still unresolved problem of psychopharmacology. Serotonin (5-HT) and brain-derived neurotrophic factor (BDNF) play a key role in the therapeutic effect of many antidepressants. Tryptophan hydroxylase 2 (TPH2) is the rate-limiting enzyme in [...] Read more.
The mechanisms of resistance to antidepressant drugs is a key and still unresolved problem of psychopharmacology. Serotonin (5-HT) and brain-derived neurotrophic factor (BDNF) play a key role in the therapeutic effect of many antidepressants. Tryptophan hydroxylase 2 (TPH2) is the rate-limiting enzyme in 5-HT synthesis in the brain. We used zebrafish (Danio rerio) as a promising model organism in order to elucidate the effect of TPH2 deficiency caused by p-chlorophenylalanine (pCPA) on the alterations in behavior and expression of 5-HT-related (Tph2, Slc6a4b, Mao, Htr1aa, Htr2aa) and BDNF-related (Creb, Bdnf, Ntrk2a, Ngfra) genes in the brain after prolonged treatment with two antidepressants, inhibitors of 5-HT reuptake (fluoxetine) and oxidation (pargyline). In one experiment, zebrafish were treated for 72 h with 0.2 mg/L fluoxetine, 2 mg/L pCPA, or the drugs combination. In another experiment, zebrafish were treated for 72 h with 0.5 mg/L pargyline, 2 mg/L pCPA, or the drugs combination. Behavior was studied in the novel tank diving test, mRNA levels were assayed by qPCR, 5-HT and its metabolite concentrations were measured by HPLC. The effects of interaction between pCPA and the drugs on zebrafish behavior were observed: pCPA attenuated “surface dwelling” induced by the drugs. Fluoxetine decreased mRNA levels of Tph2 and Htr2aa genes, while pargyline decreased mRNA levels of Slc6a4b and Htr1aa genes. Pargyline reduced Creb, Bdnf and Ntrk2a genes mRNA concentration only in the zebrafish treated with pCPA. The results show that the disruption of the TPH2 function can cause a refractory to antidepressant treatment. Full article
(This article belongs to the Special Issue Neuro-Centric Applications of Zebrafish: Models and Risk Factors)
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