Submit to IJMS Review for IJMS Propose a Special Issue

Journal Menu

Journal Browser

Molecular Advances in Platelet Function Disorders

Print Special Issue Flyer
Special Issue Editors
Special Issue Information
Keywords
Published Papers

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: closed (31 October 2022) | Viewed by 11602

Share This Special Issue

Special Issue Editor

Prof. Dr. Peter Kubisz

E-Mail Website
Guest Editor

Department of Hematology and Blood Transfusion, National Centre of Hemostasis and Thrombosis, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia
Interests: inherited and acquired thrombophilia; physiology and pathophysiology of platelets; sticky platelet syndrome; genetic polymorphisms in disorders of hemostasis; endothelial and platelet dysfunction; hemostasis and cellular therapy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

A Special Issue on the topic "Molecular Advances in Platelet Function Disorders" is being prepared for the journal IJMS. Though platelet significance in hemostasis has been recognized very soon after the initial discovery of platelets in the late 19th century, function disorders remain a problematic field. Interestingly, platelet dysfunction is a relatively common cause of clinically relevant hemorrhage. Recent data suggest the possible role of platelet dysfunction in thrombotic disorders as well. Although the acquired etiologies are predominant, congenital disorders can contribute to changes in platelet activity. This issue aims to focus on molecular mechanisms, both inherited and acquired, modifying platelet activation processes—adhesion, aggregation, and release reaction included. Original manuscripts and reviews focused on general pathophysiological and diagnostic challenges or dealing with specific disorders, both with hemorrhagic or thrombotic manifestations, are welcome from renowned experts of the field.

Prof. Dr. Peter Kubisz
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

platelets
thrombosis
hemorrhage
hemostasis
platelet aggregation

Published Papers (5 papers)

Download All Papers

Research

Jump to: Review

16 pages, 1149 KiB

Open AccessArticle

Diagnosing Czech Patients with Inherited Platelet Disorders

by Jan Louzil, Jana Stikarova, Dana Provaznikova, Ingrid Hrachovinova, Tereza Fenclova, Jan Musil, Martin Radek, Jirina Kaufmanova, Vera Geierova, Eliska Ceznerova, Peter Salaj and Roman Kotlin

Int. J. Mol. Sci. 2022, 23(22), 14386; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232214386 - 19 Nov 2022

Cited by 2 | Viewed by 1444

Abstract

A single-center study was conducted on 120 patients with inherited disorders of primary hemostasis followed at our hematological center. These patients presented a variety of bleeding symptoms; however, they had no definitive diagnosis. Establishing a diagnosis has consequences for the investigation of probands in families and for treatment management; therefore, we aimed to improve the diagnosis rate in these patients by implementing advanced diagnostic methods. According to the accepted international guidelines at the time of study, we investigated platelet morphology, platelet function assay, light-transmission aggregometry, and flow cytometry. Using only these methods, we were unable to make a definitive diagnosis for most of our patients. However, next-generation sequencing (NGS), which was applied in 31 patients, allowed us to establish definitive diagnoses in six cases (variants in ANKRD26, ITGA2B, and F8) and helped us to identify suspected variants (NBEAL2, F2, BLOC1S6, AP3D1, GP1BB, ANO6, CD36, and ITGB3) and new suspected variants (GFI1B, FGA, GP1BA, and ITGA2B) in 11 patients. The role of NGS in patients with suspicious bleeding symptoms is growing and it changes the diagnostic algorithm. The greatest disadvantage of NGS, aside from the cost, is the occurrence of gene variants of uncertain significance. Full article

(This article belongs to the Special Issue Molecular Advances in Platelet Function Disorders)

► Show Figures

Figure 1

10 pages, 1135 KiB

Open AccessCommunication

Genetic Deletion of HLJ1 Does Not Affect Blood Coagulation in Mice

by Man-Chen Hsu, Wei-Jia Luo, Bei-Chia Guo, Chia-Hui Chen, Po-An Hu, Yi-Hsuan Tsai, Kang-Yi Su and Tzong-Shyuan Lee

Int. J. Mol. Sci. 2022, 23(4), 2064; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23042064 - 13 Feb 2022

Viewed by 1692

Abstract

HLJ1 (also called DNAJB4) is a member of the DNAJ/Hsp40 family and plays an important role in regulating protein folding and activity. However, there is little information about the role of HLJ1 in the regulation of physiological function. In this study, we investigated the role of HLJ1 in blood coagulation using wild-type C57BL/6 mice and HLJ1-null (HLJ1^-/-) mice. Western blot analysis and immunohistochemistry were used to assess the expression and distribution of HLJ1 protein, respectively. The tail bleeding assay was applied to assess the bleeding time and blood loss. A coagulation test was used for measuring the activity of extrinsic, intrinsic and common coagulation pathways. Thromboelastography was used to measure the coagulation parameters in the progression of blood clot formation. The results showed that HLJ1 was detectable in plasma and bone marrow. The distribution of HLJ1 was co-localized with CD41, the marker of platelets and megakaryocytes. However, genetic deletion of HLJ1 did not alter blood loss and the activity of extrinsic and intrinsic coagulation pathways, as well as blood clot formation, compared to wild-type mice. Collectively, these findings suggest that, although HLJ1 appears in megakaryocytes and platelets, it may not play a role in the function of blood coagulation under normal physiological conditions. Full article

(This article belongs to the Special Issue Molecular Advances in Platelet Function Disorders)

► Show Figures

Figure 1

10 pages, 1056 KiB

Open AccessArticle

A Novel Mutation in GP1BB Reveals the Role of the Cytoplasmic Domain of GPIbβ in the Pathophysiology of Bernard-Soulier Syndrome and GPIb-IX Complex Assembly

by Serena Barozzi, Valeria Bozzi, Daniela De Rocco, Tania Giangregorio, Patrizia Noris, Anna Savoia and Alessandro Pecci

Int. J. Mol. Sci. 2021, 22(19), 10190; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221910190 - 22 Sep 2021

Cited by 3 | Viewed by 1961

Abstract

Bernard-Soulier syndrome (BSS) is an autosomal-recessive bleeding disorder caused by biallelic variants in the GP1BA, GP1BB, and GP9 genes encoding the subunits GPIbα, GPIbβ, and GPIX of the GPIb-IX complex. Pathogenic variants usually affect the extracellular or transmembrane domains of the receptor subunits. We investigated a family with BSS caused by the homozygous c.528_550del (p.Arg177Serfs*124) variant in GP1BB, which is the first mutation ever identified that affects the cytoplasmic domain of GPIbβ. The loss of the intracytoplasmic tail of GPIbβ results in a mild form of BSS, characterized by only a moderate reduction of the GPIb-IX complex expression and mild or absent bleeding tendency. The variant induces a decrease of the total platelet expression of GPIbβ; however, all of the mutant subunit expressed in platelets is correctly assembled into the GPIb-IX complex in the plasma membrane, indicating that the cytoplasmic domain of GPIbβ is not involved in assembly and trafficking of the GPIb-IX receptor. Finally, the c.528_550del mutation exerts a dominant effect and causes mild macrothrombocytopenia in heterozygous individuals, as also demonstrated by the investigation of a second unrelated pedigree. The study of this novel GP1BB variant provides new information on pathophysiology of BSS and the assembly mechanisms of the GPIb-IX receptor. Full article

(This article belongs to the Special Issue Molecular Advances in Platelet Function Disorders)

► Show Figures

Figure 1

20 pages, 5302 KiB

Open AccessArticle

Sample Preparation as a Critical Aspect of Blood Platelet Mitochondrial Respiration Measurements—The Impact of Platelet Activation on Mitochondrial Respiration

by Karolina Siewiera, Magdalena Labieniec-Watala, Nina Wolska, Hassan Kassassir and Cezary Watala

Int. J. Mol. Sci. 2021, 22(17), 9332; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22179332 - 28 Aug 2021

Cited by 4 | Viewed by 2133

Abstract

Blood platelets are considered as promising candidates as easily-accessible biomarkers of mitochondrial functioning. However, their high sensitivity to various stimulus types may potentially affect mitochondrial respiration and lead to artefactual outcomes. Therefore, it is crucial to identify the factors associated with platelet preparation that may lead to changes in mitochondrial respiration. A combination of flow cytometry and advanced respirometry was used to examine the effect of blood anticoagulants, the media used to suspend isolated platelets, respiration buffers, storage time and ADP stimulation on platelet activation and platelet mitochondria respiration. Our results clearly show that all the mentioned factors can affect platelet mitochondrial respiration. Briefly, (i) the use of EDTA as anticoagulant led to a significant increase in the dissipative component of respiration (LEAK), (ii) the use of plasma for the suspension of isolated platelets with MiR05 as a respiration buffer allows high electron transfer capacity and low platelet activation, and (iii) ADP stimulation increases physiological coupling respiration (ROUTINE). Significant associations were observed between platelet activation markers and mitochondrial respiration at different preparation steps; however, the fact that these relationships were not always apparent suggests that the method of platelet preparation may have a greater impact on mitochondrial respiration than the platelet activation itself. Full article

(This article belongs to the Special Issue Molecular Advances in Platelet Function Disorders)

► Show Figures

Figure 1

Review

Jump to: Research

21 pages, 1505 KiB

Open AccessReview

Impairment of Anti-Aggregatory Responses to Nitric Oxide and Prostacyclin: Mechanisms and Clinical Implications in Cardiovascular Disease

by Yuliy Y. Chirkov, Thanh H. Nguyen and John D. Horowitz

Int. J. Mol. Sci. 2022, 23(3), 1042; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23031042 - 18 Jan 2022

Cited by 14 | Viewed by 3396

Abstract

The propensity towards platelet-rich thrombus formation increases substantially during normal ageing, and this trend is mediated by decreases in platelet responsiveness to the anti-aggregatory nitric oxide (NO) and prostacyclin (PGI₂) pathways. The impairment of soluble guanylate cyclase and adenylate cyclase-based signalling that is associated with oxidative stress represents the major mechanism of this loss of anti-aggregatory reactivity. Platelet desensitization to these autacoids represents an adverse prognostic marker in patients with ischemic heart disease and may contribute to increased thrombo-embolic risk in patients with heart failure. Patients with platelet resistance to PGI₂ also are unresponsive to ADP receptor antagonist therapy. Apart from ischemia, diabetes and aortic valve disease are also associated with impaired anti-aggregatory homeostasis. This review examines the association of impaired platelet cyclic nucleotide (i.e., cGMP and cAMP) signalling with the emerging evidence of thromboembolic risk in cardiovascular diseases, and discusses the potential therapeutic strategies targeting this abnormality. Full article

(This article belongs to the Special Issue Molecular Advances in Platelet Function Disorders)

► Show Figures