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Molecular Analysis of the Resistome in Food

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Toxicology".

Deadline for manuscript submissions: closed (31 July 2021) | Viewed by 3117

Special Issue Editors


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Guest Editor
Department of Microbiology and the School of Biotechnology, and Faculty of Pharmacy, University of Santiago de Compostela, 15705 Santiago de Compostela, Spain
Interests: phage therapy; microbiology; microbial biotechnology; food microbiology; molecular microbiology; recombinant microorganisms; microbial bioactive compounds
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Guest Editor
Department of Food Technology, Spanish National Research Council (CSIC), Marine Research Institute (IIM), 36208 Vigo, Spain
Interests: proteomics; mass spectrometry; tandem mass spectrometry; proteins; food science and technology; molecular biology; food safety; confocal microscopy; food quality; food and nutrition
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Antibiotics are natural and synthetic compounds that kill bacteria and have been heavily used and abused in food for over 50 years not only to treat and prevent infections, but also to promote growth. Thus the abuse in antibiotic treatments has provoked the development and spreading of bacterial resistance and the appearance and expansion of multidrug-resistant strains in such a way that the food environment has become an important reservoir of antibiotic resistance genes/proteins and a route for their dissemination and potential transmission to human pathogens.

Recent successes of the advanced molecular methodologies based on –omics technologies (i.e. Proteomics, Genomics, Metabolomics) and systems biology approaches make them favorable strategies to analyze the resistome in food science. Thus, research institutions, agencies, food industries and regulatory laboratories are combining efforts to acquire the needed knowledge of the resistome in food.

This special issue will look at the latest advances in molecular analysis to the applications of the analysis of the resistome in food

Prof. Dr. Tomas. G. Villa
Dr. Mónica Carrera
Guest Editors

Manuscript Submission Information

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Keywords

  • Antibiotics
  • Bacteria
  • Resistome
  • Food
  • omics
  • Proteomics
  • Genomics
  • Metabolomics
  • Systems Biology

Published Papers (1 paper)

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20 pages, 366 KiB  
Article
Proteomic Characterization of Antibiotic Resistance in Listeria and Production of Antimicrobial and Virulence Factors
by Ana G. Abril, Mónica Carrera, Karola Böhme, Jorge Barros-Velázquez, Pilar Calo-Mata, Angeles Sánchez-Pérez and Tomás G. Villa
Int. J. Mol. Sci. 2021, 22(15), 8141; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22158141 - 29 Jul 2021
Cited by 8 | Viewed by 2483
Abstract
Some Listeria species are important human and animal pathogens that can be found in contaminated food and produce a variety of virulence factors involved in their pathogenicity. Listeria strains exhibiting multidrug resistance are known to be progressively increasing and that is why continuous [...] Read more.
Some Listeria species are important human and animal pathogens that can be found in contaminated food and produce a variety of virulence factors involved in their pathogenicity. Listeria strains exhibiting multidrug resistance are known to be progressively increasing and that is why continuous monitoring is needed. Effective therapy against pathogenic Listeria requires identification of the bacterial strain involved, as well as determining its virulence factors, such as antibiotic resistance and sensitivity. The present study describes the use of liquid chromatography–electrospray ionization tandem mass spectrometry (LC–ESI–MS/MS) to do a global shotgun proteomics characterization for pathogenic Listeria species. This method allowed the identification of a total of 2990 non-redundant peptides, representing 2727 proteins. Furthermore, 395 of the peptides correspond to proteins that play a direct role in Listeria pathogenicity; they were identified as virulence factors, toxins and anti-toxins, or associated with either antibiotics (involved in antibiotic-related compounds production or resistance) or resistance to toxic substances. The proteomic repository obtained here can be the base for further research into pathogenic Listeria species and facilitate the development of novel therapeutics for these pathogens. Full article
(This article belongs to the Special Issue Molecular Analysis of the Resistome in Food)
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