Submit to IJMS Review for IJMS Propose a Special Issue

Journal Menu

Journal Browser

Advances in Translational Research in Oral Tissue Regeneration

Print Special Issue Flyer
Special Issue Editors
Special Issue Information
Keywords
Published Papers

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: closed (31 October 2021) | Viewed by 10438

Share This Special Issue

Special Issue Editor

Prof. Dr. Anton Sculean

E-Mail Website
Guest Editor

Department of Periodontology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, 3010 Bern, Switzerland
Interests: oral hard and soft tissue regeneration; periodontology; implant dentistry

Special Issue Information

Dear colleagues,

Predictable regeneration of tooth-supporting tissues and of hard and soft tissue defects in the oral cavity represents a significant healthcare challenge. Since the genesis of human research endeavors in periodontology, oral surgery, and implant dentistry, the ultimate therapeutic goal has been to completely rebuild the tissues lost to the disease process or trauma and replace them with tissues that are structurally and functionally the same.

Translational research, as the name suggests, “translates” research findings from laboratory experiments into clinical application to improve the outcomes of various treatment procedures. Before translating the findings into clinical application, a proper understanding of the basic biological mechanisms involved in periodontal, bone, and oral soft tissue wound repair and regeneration are necessary to understand the molecular mechanisms that best guide tissue neogenesis during wound repair and regeneration. The transfer of basic research to clinical application requires in vitro laboratory experiments to be followed by studies in animal models to determine the safety and early-stage effectiveness of new technologies.

This Special Issue of International Journal of Molecular Sciences focuses on advances in translational research related to oral tissue regeneration as related to periodontology, oral surgery, and implant dentistry. High quality in vitro experiments or animal studies and clinical studies in addition to cutting edge reviews providing new insights on the biological mechanisms and potential clinical applications of novel technologies aimed at regenerating hard and soft oral tissues will be considered for rapid review and publication.

Prof. Dr. Anton Sculean
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

oral tissue regeneration
periodontal regeneration
bone regeneration
gingiva
oral mucosa
dental implants
in vitro studies
animal experiments
clinical applications

Published Papers (4 papers)

Download All Papers

Research

Jump to: Review

15 pages, 5251 KiB

Open AccessArticle

Effects of Obesity on Bone Healing in Rats

by Anna Damanaki, Svenja Memmert, Marjan Nokhbehsaim, Ali Abedi, Birgit Rath-Deschner, Andressa Nogueira and James Deschner

Int. J. Mol. Sci. 2021, 22(24), 13339; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222413339 - 11 Dec 2021

Cited by 6 | Viewed by 1662

Abstract

Although the association between periodontitis and obesity is well explored, it is unclear whether obesity is associated with a worse therapeutic outcome after periodontal treatment. The aim of this study was to investigate the effects of obesity on bone healing with and without the application of regeneration-promoting molecules. A standardized bone fenestration-type defect was created over the root of the mandibular first molar in 15 Wistar rats. Ten animals received a high-fat, high-sucrose diet (HFSD), while the remaining five animals were fed a standard diet. During surgery, the fenestration defects from half of the HFSD-fed, i.e., obese animals, were treated with regeneration-promoting molecules (enamel matrix derivative; EMD). After four weeks, bone healing was evaluated by histomorphometry, TRAP staining and immunohistochemistry for RUNX2 and osteopontin. The analyses revealed that the spontaneous healing of the periodontal defects was compromised by obesity. Application of EMD partially compensated for the negative effect of obesity. Nevertheless, EMD-stimulated bone healing in obese animals was not better than the spontaneous healing in the obesity-free control group, indicating that obesity may also inhibit the stimulatory effects of regeneration-promoting molecules. Our results show that obesity can negatively influence bone healing and suggest that bone healing may be compromised in humans. Full article

(This article belongs to the Special Issue Advances in Translational Research in Oral Tissue Regeneration)

► Show Figures

Figure 1

18 pages, 3618 KiB

Open AccessArticle

Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold

by Jean-Claude Imber, Andrea Roccuzzo, Alexandra Stähli, Nikola Saulacic, James Deschner, Anton Sculean and Dieter Daniel Bosshardt

Int. J. Mol. Sci. 2021, 22(20), 10915; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222010915 - 09 Oct 2021

Cited by 12 | Viewed by 2781

Abstract

(1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. New bone and cementum were labeled for bone sialoprotein, while the regenerated PDL was labeled for periostin and collagen type 1. Cytokeratin-positive epithelial cell rests of Malassez were detected in 50% of the defects. The regenerated PDL demonstrated a larger blood vessel area at the test (14.48% ± 3.52%) than at control sites (8.04% ± 1.85%, p = 0.0007). The number of blood vessels was higher in the regenerated PDL (test + control) compared to the pristine one (p = 0.012). The cell proliferative index was not statistically significantly different in pristine and regenerated PDL. (4) Conclusions: The data suggest a positive effect of VCMX on angiogenesis and an equally high cell turnover in the regenerated and pristine PDL. This VCMX supported periodontal regeneration in intrabony defects. Full article

(This article belongs to the Special Issue Advances in Translational Research in Oral Tissue Regeneration)

► Show Figures

Figure 1

20 pages, 4799 KiB

Open AccessArticle

A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts, Periodontal Ligament, and Endothelial Cells

by Maria B. Asparuhova, Alexandra Stähli, Kevin Guldener and Anton Sculean

Int. J. Mol. Sci. 2021, 22(8), 4051; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22084051 - 14 Apr 2021

Cited by 14 | Viewed by 2678

Abstract

The aim of the present study was to investigate the influence of a novel volume-stable collagen matrix (vCM) on early wound healing events including cellular migration and adhesion, protein adsorption and release, and the dynamics of the hemostatic system. For this purpose, we utilized transwell migration and crystal violet adhesion assays, ELISAs for quantification of adsorbed and released from the matrix growth factors, and qRT-PCR for quantification of gene expression in cells grown on the matrix. Our results demonstrated that primary human oral fibroblasts, periodontal ligament, and endothelial cells exhibited increased migration toward vCM compared to control cells that migrated in the absence of the matrix. Cellular adhesive properties on vCM were significantly increased compared to controls. Growth factors TGF-β1, PDGF-BB, FGF-2, and GDF-5 were adsorbed on vCM with great efficiency and continuously delivered in the medium after an initial burst release within hours. We observed statistically significant upregulation of genes encoding the antifibrinolytic thrombomodulin, plasminogen activator inhibitor type 1, thrombospondin 1, and thromboplastin, as well as strong downregulation of genes encoding the profibrinolytic tissue plasminogen activator, urokinase-type plasminogen activator, its receptor, and the matrix metalloproteinase 14 in cells grown on vCM. As a general trend, the stimulatory effect of the vCM on the expression of antifibrinolytic genes was synergistically enhanced by TGF-β1, PDGF-BB, or FGF-2, whereas the strong inhibitory effect of the vCM on the expression of profibrinolytic genes was reversed by PDGF-BB, FGF-2, or GDF-5. Taken together, our data strongly support the effect of the novel vCM on fibrin clot stabilization and coagulation/fibrinolysis equilibrium, thus facilitating progression to the next stages of the soft tissue healing process. Full article

(This article belongs to the Special Issue Advances in Translational Research in Oral Tissue Regeneration)

► Show Figures

Figure 1

Review

Jump to: Research

15 pages, 2704 KiB

Open AccessReview

Comparing Nanohydroxyapatite Graft and Other Bone Grafts in the Repair of Periodontal Infrabony Lesions: A Systematic Review and Meta-Analysis

by Muhammad Saad Shaikh, Muhammad Sohail Zafar and Ahmad Alnazzawi

Int. J. Mol. Sci. 2021, 22(21), 12021; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222112021 - 06 Nov 2021

Cited by 8 | Viewed by 2506

Abstract

Objective: To compare the results of periodontal infrabony lesions treated using nanohydroxyapatite (NcHA) graft with other bone grafts (BGs). Methods: Four electronic databases were searched including PubMed (NLM), Embase (Ovid), Medline, and Dentistry and Oral Sciences (EBSCO). The inclusion criteria included randomised controlled clinical trials (RCTs) and controlled clinical trials (CCTs). The clinical results of NcHA were compared with other BGs. For clinical attachment level (CAL) gain, probing pocket depth (PPD) decrease, and gingival recession (REC) change, weighted averages and forest plots were computed. Results: Seven RCTs fulfilled the selection criteria that were included. When NcHA was compared to other BGs, no clinically significant differences were found in terms of each outcome assessed, except the REC change for synthetic BGs as compared to NcHA. Conclusions: The use of an NcHA graft showed equivalent results compared to other types of BGs. To further validate these findings, future studies are required to compare the NcHA and various BGs over longer time periods and in furcation deficiencies. Full article

(This article belongs to the Special Issue Advances in Translational Research in Oral Tissue Regeneration)

► Show Figures