Journal Browser

Journal Browser

Topical Collection "The Use of In Vitro and In Vivo Models for the Research on Bioactive Compounds"


Dr. Diego A. Moreno
E-Mail Website
Guest Editor
1. Phytochemistry and Healthy Foods Laboratory, Department of Food Science and Technology, National Council for Scientific Research (CEBAS-CSIC), Murcia, Spain 2. Associated Unit of R&D and Innovation CEBAS-CSIC+UPCT on “Quality and Risk Assessment of Foods”, CEBAS-CSIC, Campus Espinardo - 25, E-30100 Murcia, Spain
Interests: food science and technology; phytochemistry; bioactive compounds; health-promoters, functional ingredients; natural foods; healthy foods; energy metabolism (obesity and diabetes); human nutrition; wellbeing
Special Issues and Collections in MDPI journals
Dr. Raúl Domínguez-Perles
E-Mail Website
Guest Editor
Research Group on Quality, Safety, and Bioactivity of Plant Foods, Department of Food Science and Technology, Centro de Edafología y Biología Aplicada del Segura, Spanish National Research Council (CEBAS-CSIC). University Campus of Espinardo, Edif. 25, 30100 Murcia, Spain
Interests: lipidomics; plant oxylipins; bioaccessibility; bioavailability; bioactivity; structure-activity relationship (SAR); in vitro models of biological activity; inflammation; oxidative stress; immune system
Special Issues and Collections in MDPI journals
Dr. Antonio J. Ruiz-Alcaraz
E-Mail Website
Guest Editor
1. Department of Biochemistry, Molecular Biology B and Immunology, School of Medicine, University of Murcia. Regional Campus of International Excellence “Campus Mare Nostrum”, 30100 Murcia, Spain. 2. Biomedical Research Institute of Murcia (IMIB), 30120 Murcia, Spain.
Interests: immunology; inflammation; macrophages; cell biology; in vitro models for drug development; cirrhosis; endometriosis; diabetes; insulin resistance

Topical Collection Information

Dear Colleagues,

Topical Collection gathering publications, communications, insightful and critical information, research results and opinions on the state-of-the-art investigations using in-vitro and in-vivo models as preclinical research stages for the investigation of potentially bioactive compounds.

The current challenges on the scientific validation of the functionality of bioactive compounds for health and wellbeing needs approaches and research results beyond the state-of-the-art, focused on the clarification, elucidation and investigation of the structure-function relationships and the design of better dietary and clinical interventions to back-up their potential functionality.

Besides, it would be also a great ground to discuss the findings of epidemiological and clinical studies lacking this necessary preclinical information to foster future validation of functionalities.

Many clinical results are lacking enough strength to validate a “functional” compound, ingredient or food, because of the gap of knowledge on the structure-function relationship by insufficient bioactivity demonstration, and this topical collection aims to incorporate such results for open views worldwide.

Development, establishment, and validation of in-vitro and in-vivo models for the study of health-promoting bioactive compounds at the physiological, biochemical and molecular level, in order to find the connection between the compounds in their natural matrices (extracted, purified or formulated), and the potential effects on health and wellbeing through improvements on bioaccesibility, bioavailability, metabolism, and bioactivity.

Dr. Diego A. Moreno
Dr. Raúl Domínguez-Perles
Dr. Antonio J. Ruiz-Alcaraz
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the collection website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • cell assay
  • animal models
  • preclinical
  • bioavailability
  • metabolism
  • bioactivity
  • toxicology
  • in silico models
  • immune system
  • inflammation
  • pathology
  • structure
  • function
  • molecule
  • biomarker
  • therapeutic

Published Papers (1 paper)


Open AccessArticle
Trans-Cinnamaldehyde Exhibits Synergy with Conventional Antibiotic against Methicillin-Resistant Staphylococcus aureus
Int. J. Mol. Sci. 2021, 22(5), 2752; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22052752 - 09 Mar 2021
Viewed by 356
Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen worldwide and has acquired multiple resistance to a wide range of antibiotics. Hence, there is a pressing need to explore novel strategies to overcome the increase in antimicrobial resistance. The present study aims to [...] Read more.
Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen worldwide and has acquired multiple resistance to a wide range of antibiotics. Hence, there is a pressing need to explore novel strategies to overcome the increase in antimicrobial resistance. The present study aims to investigate the efficacy and mechanism of plant-derived antimicrobials, trans-cinnamaldehyde (TCA) in decreasing MRSA’s resistance to eight conventional antibiotics. A checkerboard dilution test and time–kill curve assay are used to determine the synergistic effects of TCA combined with the antibiotics. The results indicated that TCA increased the antibacterial activity of the antibiotics by 2-16-fold. To study the mechanism of the synergism, we analyzed the mecA transcription gene and the penicillin-binding protein 2a level of MRSA treated with TCA by quantitative RT-PCR or Western blot assay. The gene transcription and the protein level were significantly inhibited. Additionally, it was verified that TCA can significantly inhibit the biofilm, which is highly resistant to antibiotics. The expression of the biofilm regulatory gene hld of MRSA after TCA treatment was also significantly downregulated. These findings suggest that TCA maybe is an exceptionally potent modulator of antibiotics. Full article
Show Figures

Figure 1

Back to TopTop