Microfluidics in Cancer Research

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B:Biology and Biomedicine".

Deadline for manuscript submissions: closed (30 September 2022) | Viewed by 7205

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Guest Editor
Istituto di Fotonica e Nanotecnologie (IFN)-CNR, Piazza Leonardo da Vinci 32, 20133 Milano, Italy
Interests: fluorescence imaging; photonics; microfluidics; biophotonics; optical manipulation of cells; lab on a chip; femtosecond laser micromachining
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Special Issue Information

Dear Colleagues,

Cancer is a leading cause of mortality worldwide, causing more than 19 million deaths in 2020. Therefore, cancer research is recognized as one of the most important fields of research in the coming years, and effort in the field is continuously growing. Microfluidics has emerged as a powerful technology for both early diagnosis and fundamental studies in cancer. Microfluidic platforms allow high sensitivity, high throughput, low reagent consumption and single-cell or intracellular analysis. These advantages represent a new frontier in deciphering tumor heterogeneity, which often causes non-responsiveness to targeted therapies. This Special Issue aims to collect the latest achievements in various aspects of microfluidics in cancer studies: new technologies and functionalities of lab-on-chip devices, measurement protocols and analysis, machine learning, and other methods for high-throughput data analysis. We invite research papers, communications, and review articles that focus on new results in the exploitation of microfluidics as a means to advance knowledge on cancer, early diagnosis and possible therapies.

Dr. Francesca Bragheri
Guest Editor

Manuscript Submission Information

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Keywords

  • lab on chip
  • integrated microsystems
  • cancer cells
  • single-cell analysis
  • microfluidics
  • optofluidics
  • high throughput

Published Papers (2 papers)

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Review

38 pages, 13030 KiB  
Review
Engineered Vasculature for Cancer Research and Regenerative Medicine
by Huu Tuan Nguyen, Arne Peirsman, Zuzana Tirpakova, Kalpana Mandal, Florian Vanlauwe, Surjendu Maity, Satoru Kawakita, Danial Khorsandi, Rondinelli Herculano, Christian Umemura, Can Yilgor, Remy Bell, Adrian Hanson, Shaopei Li, Himansu Sekhar Nanda, Yangzhi Zhu, Alireza Hassani Najafabadi, Vadim Jucaud, Natan Barros, Mehmet Remzi Dokmeci and Ali Khademhosseiniadd Show full author list remove Hide full author list
Micromachines 2023, 14(5), 978; https://0-doi-org.brum.beds.ac.uk/10.3390/mi14050978 - 29 Apr 2023
Cited by 3 | Viewed by 3161
Abstract
Engineered human tissues created by three-dimensional cell culture of human cells in a hydrogel are becoming emerging model systems for cancer drug discovery and regenerative medicine. Complex functional engineered tissues can also assist in the regeneration, repair, or replacement of human tissues. However, [...] Read more.
Engineered human tissues created by three-dimensional cell culture of human cells in a hydrogel are becoming emerging model systems for cancer drug discovery and regenerative medicine. Complex functional engineered tissues can also assist in the regeneration, repair, or replacement of human tissues. However, one of the main hurdles for tissue engineering, three-dimensional cell culture, and regenerative medicine is the capability of delivering nutrients and oxygen to cells through the vasculatures. Several studies have investigated different strategies to create a functional vascular system in engineered tissues and organ-on-a-chips. Engineered vasculatures have been used for the studies of angiogenesis, vasculogenesis, as well as drug and cell transports across the endothelium. Moreover, vascular engineering allows the creation of large functional vascular conduits for regenerative medicine purposes. However, there are still many challenges in the creation of vascularized tissue constructs and their biological applications. This review will summarize the latest efforts to create vasculatures and vascularized tissues for cancer research and regenerative medicine. Full article
(This article belongs to the Special Issue Microfluidics in Cancer Research)
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17 pages, 2785 KiB  
Review
Microfluidic Technology for the Isolation and Analysis of Exosomes
by Yusong Wu, Yuqing Wang, Yanjun Lu, Xiaomei Luo, Yinghong Huang, Ting Xie, Christian Pilarsky, Yuanye Dang and Jianye Zhang
Micromachines 2022, 13(10), 1571; https://0-doi-org.brum.beds.ac.uk/10.3390/mi13101571 - 22 Sep 2022
Cited by 15 | Viewed by 2978
Abstract
Exosomes are lipid-bilayer enclosed vesicles with diameters of 30–150 nm, which play a pivotal role in cell communication by transporting their cargoes such as proteins, lipids, and genetic materials. In recent years, exosomes have been under intense investigation, as they show great promise [...] Read more.
Exosomes are lipid-bilayer enclosed vesicles with diameters of 30–150 nm, which play a pivotal role in cell communication by transporting their cargoes such as proteins, lipids, and genetic materials. In recent years, exosomes have been under intense investigation, as they show great promise in numerous areas, especially as bio-markers in liquid biopsies. However, due to the high heterogeneity and the nano size of exosomes, the separation of exosomes is not easy. This review will deliver an outline of the conventional methods and the microfluidic-based technologies for exosome separation. Particular attention is devoted to microfluidic devices, highlighting the efficiency of exosome isolation by these methods. Additionally, this review will introduce advances made in the integrated microfluidics technologies that enable the separation and analysis of exosomes. Full article
(This article belongs to the Special Issue Microfluidics in Cancer Research)
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