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Micromachines
Special Issues
Cell Culture Microfluidic Chips
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Cell Culture Microfluidic Chips

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B2: Biofabrication and Tissue Engineering".

Deadline for manuscript submissions: closed (31 August 2021) | Viewed by 5002

Special Issue Editor

Prof. Dr. Hyuk William Choi

E-Mail Website
Guest Editor
Department of Biomedical Engineering, Korea University, Seoul 02481, Republic of Korea
Interests: organ on a chip; intervertebral disc (IVD) degeneration; photobiomodulation; electroceuticals; vagus nerve stimulation (VNS)
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Cells and tissues on a chip is a time-tested method that yields significant therapeutic benefits. Animal studies are useful in studying the complexity of an organism’s physiology, but lab-on-a-chip platforms provide superior primary models for follow-up work. What is therefore necessary are the relatively inexpensive and accessible platforms for in vitro human cell studies.

This Special Issue focuses on all topics dealing with cell culture microfluidic chips, including the multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics, and physiological response of entire organs and organ systems. It may constitute the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms.

Prof. Dr. Hyuk William Choi
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • lab chip
  • cell chip
  • tissue chip
  • organ chip
  • organoids
  • micro- and nano-sensors and actuators (physical, chemical, biological)
  • challenges and possible cell culture solutions of using MEMS technologies

Published Papers (2 papers)

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Research

16 pages, 5389 KiB  
Article
Microfluidic Chip with Low Constant-Current Stimulation (LCCS) Platform: Human Nucleus Pulposus Degeneration In Vitro Model for Symptomatic Intervertebral Disc
by An-Gi Kim, Tae-Won Kim, Woo-Keun Kwon, Kwang-Ho Lee, Sehoon Jeong, Min-Ho Hwang and Hyuk Choi
Micromachines 2021, 12(11), 1291; https://0-doi-org.brum.beds.ac.uk/10.3390/mi12111291 - 21 Oct 2021
Cited by 3 | Viewed by 2019
Abstract
Intervertebral disc (IVD) degeneration is a major cause of low back pain (LBP) in the lumbar spine. This phenomenon is caused by several processes, including matrix degradation in IVD tissues, which is mediated by matrix metalloproteinases (MMPs) and inflammatory responses, which can be [...] Read more.
Intervertebral disc (IVD) degeneration is a major cause of low back pain (LBP) in the lumbar spine. This phenomenon is caused by several processes, including matrix degradation in IVD tissues, which is mediated by matrix metalloproteinases (MMPs) and inflammatory responses, which can be mediated by interactions among immune cells, such as macrophages and IVD cells. In particular, interleukin (IL)-1 beta (β), which is a master regulator secreted by macrophages, mediates the inflammatory response in nucleus pulposus cells (NP) and plays a significant role in the development or progression of diseases. In this study, we developed a custom electrical stimulation (ES) platform that can apply low-constant-current stimulation (LCCS) signals to microfluidic chips. Using this platform, we examined the effects of LCCS on IL-1β-mediated inflammatory NP cells, administered at various currents (5, 10, 20, 50, and 100 μA at 200 Hz). Our results showed that the inflammatory response, induced by IL-1β in human NP cells, was successfully established. Furthermore, 5, 10, 20, and 100 μA LCCS positively modulated inflamed human NP cells’ morphological phenotype and kinetic properties. LCCS could affect the treatment of degenerative diseases, revealing the applicability of the LCCS platform for basic research of electroceuticals. Full article
(This article belongs to the Special Issue Cell Culture Microfluidic Chips)
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15 pages, 2098 KiB  
Article
Creating an Artificial 3-Dimensional Ovarian Follicle Culture System Using a Microfluidic System
by Mae W. Healy, Shelley N. Dolitsky, Maria Villancio-Wolter, Meera Raghavan, Alexandra R. Tillman, Nicole Y. Morgan, Alan H. DeCherney, Solji Park and Erin F. Wolff
Micromachines 2021, 12(3), 261; https://0-doi-org.brum.beds.ac.uk/10.3390/mi12030261 - 04 Mar 2021
Cited by 9 | Viewed by 2462
Abstract
We hypothesized that the creation of a 3-dimensional ovarian follicle, with embedded granulosa and theca cells, would better mimic the environment necessary to support early oocytes, both structurally and hormonally. Using a microfluidic system with controlled flow rates, 3-dimensional two-layer (core and shell) [...] Read more.
We hypothesized that the creation of a 3-dimensional ovarian follicle, with embedded granulosa and theca cells, would better mimic the environment necessary to support early oocytes, both structurally and hormonally. Using a microfluidic system with controlled flow rates, 3-dimensional two-layer (core and shell) capsules were created. The core consists of murine granulosa cells in 0.8 mg/mL collagen + 0.05% alginate, while the shell is composed of murine theca cells suspended in 2% alginate. Somatic cell viability tests and hormonal assessments (estradiol, progesterone, and androstenedione) were performed on days 1, 6, 13, 20, and 27. Confocal microscopy confirmed appropriate compartmentalization of fluorescently-labeled murine granulosa cells to the inner capsule and theca cells to the outer shell. Greater than 78% of cells present in capsules were alive up to 27 days after collection. Artificially constructed ovarian follicles exhibited intact endocrine function as evidenced by the production of estradiol, progesterone, and androstenedione. Oocytes from primary and early secondary follicles were successfully encapsulated, which maintained size and cellular compartmentalization. This novel microfluidic system successfully encapsulated oocytes from primary and secondary follicles, recapitulating the two-compartment system necessary for the development of the mammalian oocyte. Importantly, this microfluidic system can be easily adapted for sterile, high throughput applications. Full article
(This article belongs to the Special Issue Cell Culture Microfluidic Chips)
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