Microfluidics for Regenerative Medicine

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "E:Engineering and Technology".

Deadline for manuscript submissions: closed (28 February 2021) | Viewed by 3556

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Guest Editor
Research collaborator at Institute of Physics and Information Technologies, Group of Ultrasonic Resonators, CSIC, Serrano 144, 28006 Madrid, Spain
Interests: microfluidic sorting; microdroplet; microfluidic cell culture, stem cell, biomedicine
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Special Issue Information

Dear Colleagues,

The main purpose of regenerative medicine is to replace damaged human cells, tissues, or organs to restore or establish their normal function. Huge research efforts towards the development of new regenerative technologies have been made in which key players such as stem cells, soluble factors, biomaterials, or combinations thereof are involved. Nowadays, technological advancements have resulted in an increase in life expectancy, and therefore tissue and/or organ failure is inevitable in all of us as we age. To satisfy the need of damaged tissue replacement, a series of technologies providing fast and reliable assessment of biological performance to select both the potentially suitable candidates, and the early detection of poor ones, is urgently needed. Microfluidic technologies can be considered as “enabling technologies” or “tools” for accelerating research in regenerative medicine due to their well known capacity for the realization of high-throughput screening platforms. Moreover, microfluidic platforms have been demonstrated to be a unique biomimetic tool to create in vivo-like microenvironments (specifically, in the study of tissue cultures, the development of human disease culture models and hybrid technologies for assessing cell biology). Accordingly, this Special Issue seeks to showcase research papers, short communications, and review articles that focus on novel methodological developments in microfluidics applied for strategies towards regenerative medicine.

We look forward to receiving your submissions!

Dr. Pilar Carreras
Guest Editor

Manuscript Submission Information

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Keywords

  • microfluidics
  • stem cells
  • tissue engineering
  • regenerative medicine

Published Papers (1 paper)

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Research

15 pages, 4756 KiB  
Article
Long-Term Human Hematopoietic Stem Cell Culture in Microdroplets
by Pilar Carreras, Itziar González, Miguel Gallardo, Alejandra Ortiz-Ruiz, Maria Luz Morales, Jessica Encinas and Joaquín Martínez-López
Micromachines 2021, 12(1), 90; https://0-doi-org.brum.beds.ac.uk/10.3390/mi12010090 - 16 Jan 2021
Cited by 5 | Viewed by 3091
Abstract
We previously reported a new approach for micromanipulation and encapsulation of human stem cells using a droplet-based microfluidic device. This approach demonstrated the possibility of encapsulating and culturing difficult-to-preserve primary human hematopoietic stem cells using an engineered double-layered bead composed by an inner [...] Read more.
We previously reported a new approach for micromanipulation and encapsulation of human stem cells using a droplet-based microfluidic device. This approach demonstrated the possibility of encapsulating and culturing difficult-to-preserve primary human hematopoietic stem cells using an engineered double-layered bead composed by an inner layer of alginate and an outer layer of Puramatrix. We also demonstrated the maintenance and expansion of Multiple Myeloma cells in this construction. Here, the presented microfluidic technique is applied to construct a 3D biomimetic model to recapitulate the human hematopoietic stem cell niche using double-layered hydrogel beads cultured in 10% FBS culture medium. In this model, the long-term maintenance of the number of cells and expansion of hHSCS encapsulated in the proposed structures was observed. Additionally, a phenotypic characterization of the human hematopoietic stem cells generated in the presented biomimetic model was performed in order to assess their long-term stemness maintenance. Results indicate that the ex vivo cultured human CD34+ cells from bone marrow were viable, maintained, and expanded over a time span of eight weeks. This novel long-term stem cell culture methodology could represent a novel breakthrough to improve Hematopoietic Progenitor cell Transplant (HPT) as well as a novel tool for further study of the biochemical and biophysical factors influencing stem cell behavior. This technology opens a myriad of new applications as a universal stem cell niche model potentially able to expand other types of cells. Full article
(This article belongs to the Special Issue Microfluidics for Regenerative Medicine)
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