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Microorganisms
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Genus Enterococcus and Bacteriocins
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Genus Enterococcus and Bacteriocins

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: closed (30 April 2022) | Viewed by 7213

Special Issue Editors

Dr. Andrea Lauková

E-Mail Website
Guest Editor
Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Košice, Slovakia
Interests: beneficial bacteria; bacteriocins; enterococcus
Special Issues, Collections and Topics in MDPI journals
Dr. Monika Pogány Simonová

E-Mail Website
Guest Editor
Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Košice, Slovakia
Interests: beneficial bacteria and their benefit, as well as bacteriocins produced by them, especially by the representatives of the genus Enterococcus; application in food-producing but also in other animals, in food of animal origin as well as the environment (animal waste) in in vitro, in situ and in vivo studies influencing physiological parameters and meat
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The genus Enterococcus represents one of the genera frequently detected not only in animals. The view regarding enterococci is controversial because of clinical isolates. However, enterococci must be assessed from two aspects, beneficial (probiotic/beneficial strains, production of bacteriocins) and from their possibility to contaminate niches (use enterocins against contaminants). This is still an open area for studies because strain species are increasing thanks to new/progressive identification methods and individual studies focused on them. Today, “natural” is better, which allows us to use beneficial strains and their bacteriocins to maintain and/or support animal health and/or to prevent food contamination and decrease waste contamination in the environment using this approach.

Subtopics include:

  • Enterococci—benefit and/or spoilage
  • Enterococci as beneficial/probiotic strains
  • Enterococci as producers of bacteriocins
  • Enterococci and virulence factors involving antibiotic resistance
  • Treatment of enterococci with virulence factors by bacteriocins
  • Interaction studies: spoilage strains and enterocins
  • Application of beneficial enterococci and their bacteriocins in animals, food, waste

Dr. Andrea Lauková
Dr. Monika Pogány Simonová
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • enterococci
  • animals
  • benefit
  • food
  • waste
  • support health
  • enterocins
  • characteristic
  • virulence factor
  • bacteriocin genes

Published Papers (3 papers)

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Research

9 pages, 290 KiB  
Article
Enterocin 7420 and Sage in Rabbit Diet and Their Effect on Meat Mineral Content and Physico-Chemical Properties
by Monika Pogány Simonová, Ľubica Chrastinová and Andrea Lauková
Microorganisms 2022, 10(6), 1094; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms10061094 - 25 May 2022
Cited by 1 | Viewed by 1580
Abstract
Rabbit meat has outstanding nutritional characteristics—it is a lean meat with low fat, cholesterol and sodium content, with high-biological-value proteins, potassium, phosphorus, selenium, iron and vitamin B12 level. The dietary inclusion of natural bioactive compounds can improve the quality of rabbit meat. The [...] Read more.
Rabbit meat has outstanding nutritional characteristics—it is a lean meat with low fat, cholesterol and sodium content, with high-biological-value proteins, potassium, phosphorus, selenium, iron and vitamin B12 level. The dietary inclusion of natural bioactive compounds can improve the quality of rabbit meat. The present study evaluated the effect of enterocin 7420 (Ent 7420) and sage (Salvia officinalis) extract on the quality and mineral content of rabbit meat. A total of 96 Hyla rabbits (aged 35 days) were divided into E (Ent 7420; 50 µL/animal/d), S (sage extract; 10 µL/animal/d), E + S (Ent 7420 and sage in combination) and control (C) groups. Additives were administrated in drinking water for 21 days. A significant increase in meat iron (p < 0.01) content was noted; phosphorus and zinc levels were also elevated in experimental groups, compared with control data. Ent 7420 and sage treatment reduced the calcium and manganese (p < 0.01) contents. The physico-chemical traits of rabbit meat were not negatively influenced by treatment. Based on these results, diet supplementation, mostly with Ent 7420 but also in combination with sage, could enhance the quality of rabbit meat mineral, with a focus on its iron, phosphorus and zinc contents. Full article
(This article belongs to the Special Issue Genus Enterococcus and Bacteriocins)
12 pages, 1791 KiB  
Article
Characterization of the Biosynthetic Gene Cluster of Enterocin F4-9, a Glycosylated Bacteriocin
by Mohamed Abdelfattah Maky, Naoki Ishibashi, Jiro Nakayama and Takeshi Zendo
Microorganisms 2021, 9(11), 2276; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms9112276 - 01 Nov 2021
Cited by 4 | Viewed by 2649
Abstract
Enterocin F4-9 belongs to the glycocin family having post-translational modifications by two molecules of N-acetylglucosamine β-O-linked to Ser37 and Thr46. In this study, the biosynthetic gene cluster of enterocin F4-9 was cloned and expressed in Enterococcus faecalis JH2-2. Production of [...] Read more.
Enterocin F4-9 belongs to the glycocin family having post-translational modifications by two molecules of N-acetylglucosamine β-O-linked to Ser37 and Thr46. In this study, the biosynthetic gene cluster of enterocin F4-9 was cloned and expressed in Enterococcus faecalis JH2-2. Production of glycocin by the JH2-2 expression strain was confirmed by expression of the five genes. The molecular weight was greater than glycocin secreted by the wild strain, E. faecalis F4-9, because eight amino acids from the N-terminal leader sequence remained attached. This N-terminal extension was eliminated after treatment with the culture supernatant of strain F4-9, implying an extracellular protease from E. faecalis F4-9 cleaves the N-terminal sequence. Thus, leader sequences cleavage requires two steps: the first via the EnfT protease domain and the second via extracellular proteases. Interestingly, the long peptide, with N-terminal extension, demonstrated advanced antimicrobial activity against Gram-positive and Gram-negative bacteria. Furthermore, enfC was responsible for glycosylation, a necessary step prior to secretion and cleavage of the leader peptide. In addition, enfI was found to grant self-immunity to producer cells against enterocin F4-9. This report demonstrates specifications of the minimal gene set responsible for production of enterocin F4-9, as well as a new biosynthetic mechanism of glycocins. Full article
(This article belongs to the Special Issue Genus Enterococcus and Bacteriocins)
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11 pages, 1793 KiB  
Article
Enterocin Cross-Resistance Mediated by ABC Transport Systems
by Claudia Teso-Pérez, Manuel Martínez-Bueno, Juan Manuel Peralta-Sánchez, Eva Valdivia, Mercedes Maqueda, M. Esther Fárez-Vidal and Antonio M. Martín-Platero
Microorganisms 2021, 9(7), 1411; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms9071411 - 30 Jun 2021
Cited by 6 | Viewed by 2214
Abstract
In their struggle for life, bacteria frequently produce antagonistic substances against competitors. Antimicrobial peptides produced by bacteria (known as bacteriocins) are active against other bacteria, but harmless to their producer due to an associated immunity gene that prevents self-inhibition. However, knowledge of cross-resistance [...] Read more.
In their struggle for life, bacteria frequently produce antagonistic substances against competitors. Antimicrobial peptides produced by bacteria (known as bacteriocins) are active against other bacteria, but harmless to their producer due to an associated immunity gene that prevents self-inhibition. However, knowledge of cross-resistance between different types of bacteriocin producer remains very limited. The immune function of certain bacteriocins produced by the Enterococcus genus (known as enterocins) is mediated by an ABC transporter. This is the case for enterocin AS-48, a gene cluster that includes two ABC transporter-like systems (Transporter-1 and 2) and an immunity protein. Transporter-2 in this cluster shows a high similarity to the ABC transporter-like system in MR10A and MR10B enterocin gene clusters. The aim of our study was to determine the possible role of this ABC transporter in cross-resistance between these two different types of enterocin. To this end, we designed different mutants (Tn5 derivative and deletion mutants) of the as-48 gene cluster in Enterococcus faecalis and cloned them into the pAM401 shuttle vector. Antimicrobial activity assays showed that enterocin AS-48 Transporter-2 is responsible for cross-resistance between AS-48 and MR10A/B enterocin producers and allowed identification of the MR10A/B immunity gene system. These findings open the way to the investigation of resistance beyond homologous bacteriocins. Full article
(This article belongs to the Special Issue Genus Enterococcus and Bacteriocins)
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