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Microorganisms
Special Issues
Intracellular Bacteria: From Basic Research to Clinics
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Intracellular Bacteria: From Basic Research to Clinics

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Medical Microbiology".

Deadline for manuscript submissions: closed (31 December 2021) | Viewed by 8209

Special Issue Editors

Prof. Dr. Gilbert Greub

E-Mail Website
Guest Editor
Institute of Microbiology, Lausanne University Hospital, University of Lausanne, 1011 Lausanne, Switzerland
Interests: antibiotics; bacteremia; Gram's stain; Enterobacteriaceae; Gram-negative bacteria; lasers; spectrometry; mass; matrix-assisted laser desorption-ionization; mass spectrometry; pathogenic organism; gram-negative bacteremia; ionization; empirical antibiotic therapy; blood culture; extended-spectrum beta lactamases; bloodstream infections; early diagnosis; time-of-flight mass spectrometry; time-of-flight; marseilleviruses
Special Issues, Collections and Topics in MDPI journals
Dr. Nicolas Jacquier

E-Mail Website
Guest Editor
Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
Interests: antibiotics; bacteremia; Gram's stain; enterobacteriaceae; Gram-negative bacteria; lasers; spectrometry; mass; matrix-assisted laser desorption-ionization; mass spectrometry; pathogenic organism; Gram-negative bacteremia; ionization; empirical antibiotic therapy; blood culture; extended-spectrum beta lactamases; bloodstream infections; early diagnosis; time-of-flight mass spectrometry; time-of-flight; marseilleviruses
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In August 2022, Lausanne (Switzerland) will welcome a joint ESCCAR (European Society on Chlamydia, Coxiella, Anaplasma and Rickettsia)–ESCR (European Society for Chlamydia Research) international meeting. Intracellular bacteria, such as Chlamydia, Coxiella, Anaplasma and Rickettsia, are relevant human pathogens. This meeting will thus address several important subjects regarding intracellular bacteria, such as diagnosis, epidemiology, pathogenesis, immunology, treatments, as well as the fundamental research performed on these bacteria, comprising host–cell interactions, genetics, evolution and cell biology, among others.

The organization of this meeting is a good opportunity to launch a Special Issue of Microorganisms, which will allow people in the field of intracellular bacteria to share their recent discoveries and/or opinions. Original research articles or reviews that bring a better understanding of the diverse aspects of intracellular bacteria are thus welcome for this Special Issue.

Dr. Gilbert Greub
Dr. Nicolas Jacquier
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Chlamydia
  • Coxiella
  • Anaplasma
  • Rickettsia
  • intracellular bacteria
  • host–cell interaction
  • pathogenicity
  • medical microbiology

Published Papers (3 papers)

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Research

11 pages, 1189 KiB  
Article
Development of High-Throughput Multiplex Serology to Detect Serum Antibodies against Coxiella burnetii
by Rima Jeske, Larissa Dangel, Leander Sauerbrey, Dimitrios Frangoulidis, Lauren R. Teras, Silke F. Fischer and Tim Waterboer
Microorganisms 2021, 9(11), 2373; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms9112373 - 17 Nov 2021
Cited by 3 | Viewed by 1431
Abstract
The causative agent of Q fever, the bacterium Coxiella burnetii (C. burnetii), has gained increasing interest due to outbreak events and reports about it being a potential risk factor for the development of lymphomas. In order to conduct large-scale studies for [...] Read more.
The causative agent of Q fever, the bacterium Coxiella burnetii (C. burnetii), has gained increasing interest due to outbreak events and reports about it being a potential risk factor for the development of lymphomas. In order to conduct large-scale studies for population monitoring and to investigate possible associations more closely, accurate and cost-effective high-throughput assays are highly desired. To address this need, nine C. burnetii proteins were expressed as recombinant antigens for multiplex serology. This technique enables the quantitative high-throughput detection of antibodies to multiple antigens simultaneously in a single reaction. Based on a reference group of 76 seropositive and 91 seronegative sera, three antigens were able to detect C. burnetii infections. Com1, GroEL, and DnaK achieved specificities of 93%, 69%, and 77% and sensitivities of 64%, 72%, and 47%, respectively. Double positivity to Com1 and GroEL led to a combined specificity of 90% and a sensitivity of 71%. In a subgroup of seropositives with an increased risk for chronic Q fever, the double positivity to these markers reached a specificity of 90% and a sensitivity of 86%. Multiplex serology enables the detection of antibodies against C. burnetii and appears well-suited to investigate associations between C. burnetii infections and the clinical manifestations in large-scale studies. Full article
(This article belongs to the Special Issue Intracellular Bacteria: From Basic Research to Clinics)
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18 pages, 2482 KiB  
Article
Virulence Comparison of Salmonella enterica Subsp. enterica Isolates from Chicken and Whole Genome Analysis of the High Virulent Strain S. Enteritidis 211
by Luqing Cui, Xiangru Wang, Yue Zhao, Zhong Peng, Pan Gao, Zhengzheng Cao, Jiawei Feng, Fan Zhang, Kaixuan Guo, Min Wu, Huanchun Chen and Menghong Dai
Microorganisms 2021, 9(11), 2239; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms9112239 - 28 Oct 2021
Cited by 7 | Viewed by 2687
Abstract
Background: Salmonellaenterica is one of the common pathogens in both humans and animals that causes salmonellosis and threatens public health all over the world. Methods and Results: Here we determined the virulence phenotypes of nine Salmonellaenterica subsp. enterica (S. enterica [...] Read more.
Background: Salmonellaenterica is one of the common pathogens in both humans and animals that causes salmonellosis and threatens public health all over the world. Methods and Results: Here we determined the virulence phenotypes of nine Salmonellaenterica subsp. enterica (S. enterica) isolates in vitro and in vivo, including pathogenicity to chicken, cell infection, biofilm formation and virulence gene expressions. S. Enteritidis 211 (SE211) was highly pathogenic with notable virulence features among the nine isolates. The combination of multiple virulence genes contributed to the conferring of the high virulence in SE211. Importantly, many mobile genetic elements (MGEs) were found in the genome sequence of SE211, including a virulence plasmid, genomic islands, and prophage regions. The MGEs and CRISPR-Cas system might function synergistically for gene transfer and immune defense. In addition, the neighbor joining tree and the minimum spanning tree were constructed in this study. Conclusions: This study provided both the virulence phenotypes and genomic features, which might contribute to the understanding of bacterial virulence mechanisms in Salmonella enterica subsp. enterica. The first completed genomic sequence for the high virulent S. Enteritidis isolate SE211 and the comparative genomics and phylogenetic analyses provided a preliminary understanding of S. enterica genetics and laid the foundation for further study. Full article
(This article belongs to the Special Issue Intracellular Bacteria: From Basic Research to Clinics)
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16 pages, 8575 KiB  
Article
Behavioral and Neurochemical Shifts at the Hippocampus and Frontal Cortex Are Associated to Peripheral Inflammation in Balb/c Mice Infected with Brucella abortus 2308
by José Luis Maldonado-García, Gilberto Pérez-Sánchez, Enrique Becerril Villanueva, Samantha Alvarez-Herrera, Lenin Pavón, Gabriel Gutiérrez-Ospina, Rubén López-Santiago, Jesús Octavio Maldonado-Tapia, Sonia Mayra Pérez-Tapia and Martha C. Moreno-Lafont
Microorganisms 2021, 9(9), 1937; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms9091937 - 11 Sep 2021
Cited by 4 | Viewed by 3081
Abstract
Brucellosis is a zoonosis affecting 50,000,000 people annually. Most patients progress to a chronic phase of the disease in which neuropsychiatric symptoms upsurge. The biological processes underlying the progression of these symptoms are yet unclear. Peripheral inflammation mounted against Brucella may condition neurochemical [...] Read more.
Brucellosis is a zoonosis affecting 50,000,000 people annually. Most patients progress to a chronic phase of the disease in which neuropsychiatric symptoms upsurge. The biological processes underlying the progression of these symptoms are yet unclear. Peripheral inflammation mounted against Brucella may condition neurochemical shifts and hence unchained neuropsychiatric disorders. Our work aimed at establishing whether neurological, behavioral, and neurochemical disarrays are circumstantially linked to peripheral inflammation uprise secondary to Brucella abortus 2308 infections. We then evaluated, in control and Brucella-infected mice, skeletal muscle strength, movement coordination, and balance and motivation, as well as dopamine, epinephrine, norepinephrine, and serotonin availability in the cerebellum, frontal cortex, and hippocampus. Serum levels of proinflammatory cytokines and corticosterone in vehicle-injected and -infected mice were also estimated. All estimates were gathered at the infection acute and chronic phases. Our results showed that infected mice displayed motor disabilities, muscular weakness, and reduced motivation correlated with neurochemical and peripheral immunological disturbances that tended to decrease after 21 days of infection. The present observations support that disturbed peripheral inflammation and the related neurochemical disruption might lead to mood disorders in infected mice. Future experiments must be aimed at establishing causal links and to explore whether similar concepts might explain neurological and mood disorders in humans affected by brucellosis. Full article
(This article belongs to the Special Issue Intracellular Bacteria: From Basic Research to Clinics)
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