Understanding Phage Particles 2.0

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Molecular Microbiology and Immunology".

Deadline for manuscript submissions: closed (31 December 2022) | Viewed by 4165

Special Issue Editor


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Guest Editor
Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Spain
Interests: bacteriophages; endolysins; antimicrobials; transduction
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Special Issue Information

Dear Colleagues,

This Special Issue is the continuation of our previous special issue "Understanding Phage Particles".

Bacteriophages, or phages, are viruses that only infect and kill bacteria. They are the most abundant biological entities on earth, occupying all ecological niches where they have a decisive role in the balance and evolution of bacterial ecosystems. Phages shape bacterial population dynamics and can significantly alter both intra- and interspecific competition among their bacterial hosts. Phage particles are vehicles for horizontal gene transfer, and many bacterial virulence and fitness factors are encoded by phages or phage-like elements. Advances in phage biology research have led to the study of these phage–host interactions and their exploitation to obtain phages and phage-based products for their application in different settings, such as agricultural and food industry as well as in clinical settings.

With this Special Issue, we want to provide an update on those phage–host interactions, especially, but not limited to, interactions involving horizontal gene transfer, modification of the bacterial fitness, the role of phage particles as reservoirs of bacterial genes, their role as drivers of evolution for bacterial communities, as well as an update on the potential of phages and phage-based products for phage therapy and other applications.

Dr. Lorena Rodriguez-Rubio
Guest Editor

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Keywords

  • bacteriophages
  • transduction
  • horizontal gene transfer
  • coevolution
  • bacterial fitness
  • phage therapy

Published Papers (2 papers)

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Research

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26 pages, 8879 KiB  
Article
Characterization of a Lytic Bacteriophage and Demonstration of Its Combined Lytic Effect with a K2 Depolymerase on the Hypervirulent Klebsiella pneumoniae Strain 52145
by Botond Zsombor Pertics, Tamás Kovács and György Schneider
Microorganisms 2023, 11(3), 669; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms11030669 - 06 Mar 2023
Cited by 2 | Viewed by 2177
Abstract
Klebsiella pneumoniae is a nosocomial pathogen. Among its virulence factors is the capsule with a prominent role in defense and biofilm formation. Bacteriophages (phages) can evoke the lysis of bacterial cells. Due to the mode of action of their polysaccharide depolymerase enzymes, phages [...] Read more.
Klebsiella pneumoniae is a nosocomial pathogen. Among its virulence factors is the capsule with a prominent role in defense and biofilm formation. Bacteriophages (phages) can evoke the lysis of bacterial cells. Due to the mode of action of their polysaccharide depolymerase enzymes, phages are typically specific for one bacterial strain and its capsule type. In this study, we characterized a bacteriophage against the capsule-defective mutant of the nosocomial K. pneumoniae 52145 strain, which lacks K2 capsule. The phage showed a relatively narrow host range but evoked lysis on a few strains with capsular serotypes K33, K21, and K24. Phylogenetic analysis showed that the newly isolated Klebsiella phage 731 belongs to the Webervirus genus in the Drexlerviridae family; it has a 31.084 MDa double-stranded, linear DNA with a length of 50,306 base pairs and a G + C content of 50.9%. Out of the 79 open reading frames (ORFs), we performed the identification of orf22, coding for a trimeric tail fiber protein with putative capsule depolymerase activity, along with the mapping of other putative depolymerases of phage 731 and homologous phages. Efficacy of a previously described recombinant K2 depolymerase (B1dep) was tested by co-spotting phage 731 on K. pneumoniae strains, and it was demonstrated that the B1dep-phage 731 combination allows the lysis of the wild type 52145 strain, originally resistant to the phage 731. With phage 731, we showed that B1dep is a promising candidate for use as a possible antimicrobial agent, as it renders the virulent strain defenseless against other phages. Phage 731 alone is also important due to its efficacy on K. pneumoniae strains possessing epidemiologically important serotypes. Full article
(This article belongs to the Special Issue Understanding Phage Particles 2.0)
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Review

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12 pages, 1125 KiB  
Review
The Novel Role of Phage Particles in Chronic Liver Diseases
by Liuying Chen, Xiaohua Hou and Huikuan Chu
Microorganisms 2023, 11(5), 1181; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms11051181 - 30 Apr 2023
Cited by 1 | Viewed by 1445
Abstract
The gut microbiome is made up of bacteria, fungi, viruses and archaea, all of which are closely related with human health. As the main component of enterovirus, the role of bacteriophages (phages) in chronic liver disease has been gradually recognized. Chronic liver diseases, [...] Read more.
The gut microbiome is made up of bacteria, fungi, viruses and archaea, all of which are closely related with human health. As the main component of enterovirus, the role of bacteriophages (phages) in chronic liver disease has been gradually recognized. Chronic liver diseases, including alcohol-related liver disease and nonalcoholic fatty liver disease, exhibit alterations of the enteric phages. Phages shape intestinal bacterial colonization and regulate bacterial metabolism. Phages adjoining to intestinal epithelial cells prevent bacteria from invading the intestinal barrier, and mediate intestinal inflammatory response. Phages are also observed increasing intestinal permeability and migrating to peripheral blood and organs, likely contributing to inflammatory injury in chronic liver diseases. By preying on harmful bacteria, phages can improve the gut microbiome of patients with chronic liver disease and thus act as an effective treatment method. Full article
(This article belongs to the Special Issue Understanding Phage Particles 2.0)
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