Special Issue "Probiotics and Their Metabolism"

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: closed (31 May 2022) | Viewed by 2312

Special Issue Editor

Prof. Dr. Gianluigi Mauriello
E-Mail Website
Guest Editor
Division of Microbiology, Department of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055 Portici (Napoli), Italy
Interests: microencapsulation; probiotic foods; antimicrobial packaging
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

A great deal of research has been done on probiotic microorganisms. However, only a fraction of the potential of probiotics to enhance human health has been explored. This is because microbial metabolism is a complex field of study and it is difficult to understand all the existing correlations between microorganisms and humans. Moreover, the concept behind the term “probiotic” is evolving, and more microorganisms, together with the traditional probiotics, are the subject of new investigations to better understand the role of microbial metabolism in human health. This is the field of the next-generation probiotics, usually indicated as NGPs. Furthermore, a new field related to probiotics has recently been proposed—namely, postbiotics, in which the metabolism of probiotics, even though outside of the host, is of primary interest. In this scenario, any investigation on the metabolism of probiotics, directly or indirectly finalized to the enhancement of human health, is welcome in this Special Issue.

Prof. Dr. Gianluigi Mauriello
Guest Editor

Manuscript Submission Information

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Keywords

  • bacteriocins
  • bile salt deconjugation
  • short chain fatty acids
  • antibiotic resistance
  • gastrointestinal transit
  • microencapsulation
  • probiotic genes

Published Papers (3 papers)

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Research

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Article
Effects of Spore-Displayed p75 Protein from Lacticaseibacillus rhamnosus GG on the Transcriptional Response of HT-29 Cells
Microorganisms 2022, 10(7), 1276; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms10071276 - 23 Jun 2022
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Abstract
A Lacticaseibacillus rhamnosus GG-derived protein, p75, is one of the key molecules exhibiting probiotic activity. However, the molecular mechanism and transcriptional response of p75 in human intestinal epithelial cells are not completely understood. To gain a deeper understanding of its potential probiotic action, [...] Read more.
A Lacticaseibacillus rhamnosus GG-derived protein, p75, is one of the key molecules exhibiting probiotic activity. However, the molecular mechanism and transcriptional response of p75 in human intestinal epithelial cells are not completely understood. To gain a deeper understanding of its potential probiotic action, this study investigated genome-wide responses of HT-29 cells to stimulation by spore-displayed p75 (CotG-p75) through a transcriptome analysis based on RNA sequencing. Analysis of RNA-seq data showed significant changes of gene expression in HT-29 cells stimulated by CotG-p75 compared to the control. A total of 189 up-regulated and 314 down-regulated genes was found as differentially expressed genes. Gene ontology enrichment analysis revealed that a large number of activated genes was involved in biological processes, such as epithelial cell differentiation, development, and regulation of cell proliferation. A gene–gene interaction network analysis showed that several DEGs, including AREG, EREG, HBEGF, EPGN, FASLG, GLI2, CDKN1A, FOSL1, MYC, SERPINE1, TNFSF10, BCL6, FLG, IVL, SPRR1A, SPRR1B, SPRR3, and MUC5AC, might play a critical role in these biological processes. RNA-seq results for selected genes were verified by reverse transcription-quantitative polymerase chain reaction. Overall, these results provide extensive knowledge about the transcriptional responses of HT-29 cells to stimulation by CotG-p75. This study showed that CotG-p75 can contribute to cell survival and epithelial development in human intestinal epithelial cells. Full article
(This article belongs to the Special Issue Probiotics and Their Metabolism)
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Article
Putrescine Production by Latilactobacillus curvatus KP 3-4 Isolated from Fermented Foods
Microorganisms 2022, 10(4), 697; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms10040697 - 24 Mar 2022
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Abstract
Polyamines are aliphatic hydrocarbons with terminal amino groups and are essential for biological activities. It has been reported that polyamines have health-promoting effects in animals, such as the extension of lifespan by polyamine intake. The identification of a high polyamine-producing bacterium from foods [...] Read more.
Polyamines are aliphatic hydrocarbons with terminal amino groups and are essential for biological activities. It has been reported that polyamines have health-promoting effects in animals, such as the extension of lifespan by polyamine intake. The identification of a high polyamine-producing bacterium from foods could lead to the development of a novel probiotic candidate. We aimed to identify high polyamine-producing bacteria from food, and isolated and collected bacteria from vegetables and fermented foods produced in Japan. We successfully acquired Latilactobacillus curvatus KP 3-4 isolated from Kabura-zushi as a putrescine producing lactic acid bacteria. Comparing the polyamine synthesis capability of L. curvatus KP 3-4 with that of typical probiotic lactic acid bacteria and L. curvatus strains available from the Japan Collection of Microorganisms, it was found that only L. curvatus KP 3-4 was capable of exporting high levels of putrescine into the culture supernatant. The enhancement of putrescine production by the addition of ornithine, and whole-genome analysis of L. curvatus KP 3-4, suggest that putrescine is synthesized via ornithine decarboxylase. The administration of L. curvatus KP 3-4 to germ-free mice increased the concentration of putrescine in the feces. Full article
(This article belongs to the Special Issue Probiotics and Their Metabolism)
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Review

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Review
Limosilactobacillus reuteri in Health and Disease
Microorganisms 2022, 10(3), 522; https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms10030522 - 28 Feb 2022
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Abstract
Limosilactobacillus reuteri is a microorganism with valuable probiotic qualities that has been widely employed in humans to promote health. It is a well-studied probiotic bacterium that exerts beneficial health effects due to several metabolic mechanisms that enhance the production of anti-inflammatory cytochines and [...] Read more.
Limosilactobacillus reuteri is a microorganism with valuable probiotic qualities that has been widely employed in humans to promote health. It is a well-studied probiotic bacterium that exerts beneficial health effects due to several metabolic mechanisms that enhance the production of anti-inflammatory cytochines and modulate the gut microbiota by the production of antimicrobial molecules, including reuterin. This review provides an overview of the data that support the role of probiotic properties, and the antimicrobial and immunomodulatory effects of some L. reuteri strains in relation to their metabolite production profile on the amelioration of many diseases and disorders. Although the results discussed in this paper are strain dependent, they show that L. reuteri, by different mechanisms and various metabolites, may control body weight and obesity, improve insulin sensitivity and glucose homeostasis, increase gut integrity and immunomodulation, and attenuate hepatic disorders. Gut microbiota modulation by ingesting probiotic L. reuteri strains could be a promising preventative and therapeutic approach against many diseases and disorders. Full article
(This article belongs to the Special Issue Probiotics and Their Metabolism)
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