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Mass Spectrometry and Capillary Electrophoresis for the Analysis of Amino-Acids, Peptides and Proteins

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: closed (30 October 2021) | Viewed by 2786

Special Issue Editor


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Guest Editor
Laboratory for the Analysis of Medicines (LAM), Department of Pharmacy, CIRM, University of Liege, Place Du 20 Août 7, 4000 Liège, Belgium
Interests: microfluidics; capillary electrophoresis; mass spectrometry; ion-mobility; quality control of medicines; biomarkers;

Special Issue Information

Dear Colleagues,

Last decade marked significant improvements into interfaces for the coupling of capillary electrophoresis to mass spectrometry, which open new avenue in the field of biochemistry and biomedical sciences. Capillary electrophoresis brings high versatility and high separation power. Its coupling to mass spectrometry provides an additional dimension in terms of selectivity, sensitivity and characterization. Not only amino-acids, peptides but also native proteins or even complexes can finely be interrogated leading to significant progress in fundamental (i.e. protein-ligand and protein-protein interactions), applied (i.e. quality control of medicines) and clinical (i.e. discovery and quantification of biomarkers) areas.

In this context, it is a great pleasure to invite you to contribute to this thematic special issue of Molecules on Mass Spectrometry and Capillary Electrophoresis for the Analysis of Amino-acids, Peptides and Proteins that will cover the latest research trends and applications in the field. Submissions of review and original research papers are highly welcome. For review articles, a brief summary is required as preliminary step to avoid overlapping of topics.

I am confident that with your contribution to this special issue will be an important document in this fast-evolving and exiting field.

Prof. Dr. Marianne Fillet
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Capillary electrophoresis
  • Mass spectrometry
  • Amino-acids
  • Peptides
  • Proteins
  • Quantification
  • Characterization
  • Proteomics
  • On-line concentration
  • CE-MS interface
  • Ion mobility
  • Protein-ligand interactions
  • Protein-protein interaction
  • Automation

Published Papers (1 paper)

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Research

13 pages, 1693 KiB  
Article
Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS
by Emmanouil Mavrogeorgis, Harald Mischak, Agnieszka Latosinska, Justyna Siwy, Vera Jankowski and Joachim Jankowski
Molecules 2021, 26(23), 7260; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules26237260 - 30 Nov 2021
Cited by 24 | Viewed by 2349
Abstract
In recent years, capillary electrophoresis coupled to mass spectrometry (CE-MS) has been increasingly applied in clinical research especially in the context of chronic and age-associated diseases, such as chronic kidney disease, heart failure and cancer. Biomarkers identified using this technique are already used [...] Read more.
In recent years, capillary electrophoresis coupled to mass spectrometry (CE-MS) has been increasingly applied in clinical research especially in the context of chronic and age-associated diseases, such as chronic kidney disease, heart failure and cancer. Biomarkers identified using this technique are already used for diagnosis, prognosis and monitoring of these complex diseases, as well as patient stratification in clinical trials. CE-MS allows for a comprehensive assessment of small molecular weight proteins and peptides (<20 kDa) through the combination of the high resolution and reproducibility of CE and the distinct sensitivity of MS, in a high-throughput system. In this study we assessed CE-MS analytical performance with regards to its inter- and intra-day reproducibility, variability and efficiency in peptide detection, along with a characterization of the urinary peptidome content. To this end, CE-MS performance was evaluated based on 72 measurements of a standard urine sample (60 for inter- and 12 for intra-day assessment) analyzed during the second quarter of 2021. Analysis was performed per run, per peptide, as well as at the level of biomarker panels. The obtained datasets showed high correlation between the different runs, low variation of the ten highest average individual log2 signal intensities (coefficient of variation, CV < 10%) and very low variation of biomarker panels applied (CV close to 1%). The findings of the study support the analytical performance of CE-MS, underlining its value for clinical application. Full article
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