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Carbohydrate Metabolism as Target in Infectious Disease

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Medicinal Chemistry".

Deadline for manuscript submissions: closed (31 August 2022) | Viewed by 2988

Special Issue Editors

CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale Et Fonctionnelle, INSERM U1285, 59000 Lille, France
Interests: fungal infection; mycology; parasitology; infectious diseases; clinical immunology; yeasts; pathogens; microbiology; medical microbiology; fungal biology
INSERM U1285, CNRS UMR 8576–UGSF–Unité de Glycobiologie Structurale et Fonctionnelle, University of Lille, 59000 Lille, France
Interests: organic chemistry; organometalic chemistry; medical chemistry; infectious diseases; antifungal; chemobiology

Special Issue Information

Dear Colleagues,

Infection of humans (or other mammals) can be caused by different organisms such as bacteria, fungi, parasites or viruses. Each infectious disease presents its own specific signs and symptoms, although some symptoms are common to different diseases. On the other hand, infectious pathogens can be responsible for minor infections such as colds, yeast infections or serious infections that can cause death, such as AIDS, invasive candidemia, malaria, septicaemia or tuberculosis.

Within this wide range of infectious diseases, patient management problems have been identified around the world, whatever the type of disease; for example, problems of resistance to current therapies, a lack of effective diagnostic tools, and high treatment costs causing real concerns for access to care in third world countries.

These different problems have led scientific society to innovate in the development of tools specific to infectious diseases. Indeed, many mechanisms are involved in multiple adaptation processes during infection, such as specific adherence of the pathogen to host cells and tissues, defence and escape against the host’s immune system, damage to host cells and tissues. In order to survive and efficiently replicate in host cells, intracellular pathogens must adapt their metabolism to the physical conditions or available nutrients. Among the different pathogens’ constituents, simple and complex carbohydrates (glycans) have long been known to play a significant role in metabolic, structural and physical roles in biological systems.

The investigation of the molecular basis of these biological events represents an infinite source of inspiration in the field of the fight against infectious diseases.

This Special Issue is devoted to studies on the characterization and modulation of carbohydrate metabolism in infectious diseases. Contributions to any aspect of these fields are welcome, e.g. structural studies, the identification of a new carbohydrate metabolism pathway, structure–activity relationships, the design and synthesis of inhibitors, computational approaches and alternatives animal models used in carbohydrate metabolism studies.

Prof. Dr. Boualem Sendid
Dr. Faustine Dubar
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Infectious diseases
  • Carbohydrate
  • Glycan
  • Metabolism
  • Inhibitor
  • Synthesis
  • Medicinal chemistry
  • Structure-activity relationship
  • Structure-based inhibitor design
  • Application
  • Therapeutic target
  • identification
  • Sugar transporter

Published Papers (1 paper)

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Research

13 pages, 2330 KiB  
Article
Purifying and Characterizing Bacterially Expressed Soluble Lactate Dehydrogenase from Plasmodium knowlesi for the Development of Anti-Malarial Drugs
by Nurhainis Ogu Salim, Fazia Adyani Ahmad Fuad, Farahayu Khairuddin, Wan Mohd Khairulikhsan Wan Seman and Mohd Anuar Jonet
Molecules 2021, 26(21), 6625; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules26216625 - 01 Nov 2021
Cited by 4 | Viewed by 2336
Abstract
Plasmodium lactate dehydrogenase (pLH) is one of the enzymes in glycolysis with potential target for chemotherapy. This study aimed to clone, overexpress and characterize soluble recombinant lactate dehydrogenase from Plasmodium knowlesi in a bacterial system. Synthetic P. knowlesi lactate dehydrogenase (Pk-LDH) [...] Read more.
Plasmodium lactate dehydrogenase (pLH) is one of the enzymes in glycolysis with potential target for chemotherapy. This study aimed to clone, overexpress and characterize soluble recombinant lactate dehydrogenase from Plasmodium knowlesi in a bacterial system. Synthetic P. knowlesi lactate dehydrogenase (Pk-LDH) gene was cloned into pET21a expression vector, transformed into Escherichia coli strain BL21 (DE3) expression system and then incubated for 18 h, 20 °C with the presence of 0.5 mM isopropyl β-d-thiogalactoside in Terrific broth supplemented with Magnesium sulfate, followed by protein purifications using Immobilized Metal Ion Affinity Chromatography and size exclusion chromatography (SEC). Enzymatic assay was conducted to determine the activity of the enzyme. SDS-PAGE analysis revealed that protein of 34 kDa size was present in the soluble fraction. In SEC, a single peak corresponding to the size of Pk-LDH protein was observed, indicating that the protein has been successfully purified. From MALDI-TOF analysis findings, a peptide score of 282 was established, which is significant for lactate dehydrogenase from P. knowlesi revealed via MASCOT analysis. Secondary structure analysis of CD spectra indicated 79.4% α helix and 1.37% β strand structure. Specific activity of recombinant Pk-LDH was found to be 475.6 U/mg, confirming the presence of active protein. Soluble Pk-LDH that is biologically active was produced, which can be used further in other malaria studies. Full article
(This article belongs to the Special Issue Carbohydrate Metabolism as Target in Infectious Disease)
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