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Special Issue Editors

Prof. Dr. Claudia Cimpoiu

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Guest Editor

Faculty of Chemistry and Chemical Engineering, Universitatea Babes-Bolyai din Cluj-Napoca, Cluj Napoca, Romania
Interests: analytical chemistry; chromatography; food analysis; phytopharmaceutical analysis; biological activities determination; method validation

Prof. Dr. Lukasz Komsta

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Co-Guest Editor

Faculty of Pharmacy, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland
Interests: chemometrics; chemoinformatics; chromatography; pharmaceutical analysis; pharmacology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

A wide variety of compounds with biological activities that can be used in medicine and in the pharmaceutical, food, and cosmetic industries are found in nature. Therefore, this Special Issue aims to gather the most recent contributions regarding chromatographic bioassays used for analyzing and testing the various biological activities of such compounds. The articles can cover all chromatographic techniques, including chemometrics and DoE approaches. Additionally, the elucidation of the individual molecular bioactivities of the compounds by well-established and advanced bio-chromatographic techniques is welcome.

Prof. Dr. Claudia Cimpoiu
Prof. Dr. Lukasz Komsta
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

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Keywords

Natural compounds
Biological activity
Chromatography
Bioassays
Analysis
Molecular recognition
Chemometrics

Published Papers (2 papers)

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Research

Jump to: Review

9 pages, 1996 KiB

Open AccessArticle

Bioassay-Guided Isolation of Two Eudesmane Sesquiterpenes from Lindera strychnifolia Using Centrifugal Partition Chromatography

by Ji Hoon Kim, Je-Seung Jeon, Jung Hoon Kim, Eun Ju Jung, Yun Jung Lee, En Mei Gao, Ahmed Shah Syed, Rak Ho Son and Chul Young Kim

Molecules 2021, 26(17), 5269; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules26175269 - 30 Aug 2021

Cited by 4 | Viewed by 1771

Abstract

In this study, a centrifugal partition chromatography (CPC) separation was applied to identify antioxidant-responsive element (ARE) induction molecules from the crude extract of Lindera strychnifolia roots. CPC was operated with a two-phase solvent system composed of n-hexane-methanol-water (10:8.5:1.5, v/v/v) in dual mode (descending to ascending), which provided a high recovery rate (>95.5%) with high resolution. Then, ARE induction activity of obtained CPC fractions was examined in ARE-transfected HepG2 cells according to the weight ratios of the obtained fractions. The fraction exhibiting ARE-inducing activity was further purified by preparative HPLC that led to isolation of two eudesmane type sesquiterpenes as active compounds. The chemical structures were elucidated as linderolide U (1) and a new sesquiterpene named as linderolide V (2) by spectroscopic data. Further bioactivity test demonstrated that compounds 1 and 2 enhanced ARE activity by 22.4-fold and 7.6-fold, respectively, at 100 μM concentration while 5 μM of sulforaphane induced ARE activity 24.8-fold compared to the control. Full article

(This article belongs to the Special Issue Chromatography: Determination of the Biological Activity of Natural Compounds)

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Review

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25 pages, 2687 KiB

Open AccessReview

Analytical, Preparative, and Industrial-Scale Separation of Substances by Methods of Countercurrent Liquid-Liquid Chromatography

by Artak A. Kostanyan, Andrey A. Voshkin and Vera V. Belova

Molecules 2020, 25(24), 6020; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules25246020 - 18 Dec 2020

Cited by 15 | Viewed by 3288

Abstract

Countercurrent liquid-liquid chromatographic techniques (CCC), similar to solvent extraction, are based on the different distribution of compounds between two immiscible liquids and have been most widely used in natural product separations. Due to its high load capacity, low solvent consumption, the diversity of separation methods, and easy scale-up, CCC provides an attractive tool to obtain pure compounds in the analytical, preparative, and industrial-scale separations. This review focuses on the steady-state and non-steady-state CCC separations ranging from conventional CCC to more novel methods such as different modifications of dual mode, closed-loop recycling, and closed-loop recycling dual modes. The design and modeling of various embodiments of CCC separation processes have been described. Full article

(This article belongs to the Special Issue Chromatography: Determination of the Biological Activity of Natural Compounds)

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