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LC-MS/MS Method for Metabolomics and Proteomics

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: closed (15 August 2021) | Viewed by 4278

Special Issue Editor

Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019, USA
Interests: metabolomics; LC-MS/MS; HPLC; high resolution mass spectrometry; gas phase ion chemistry; method development; small molecule quantitation; CID; HCD; PRM; chagas diseases; peptide sequencing; fragmentation mechanism; biomarker; liver cancer; photoelectron spectroscopy

Special Issue Information

Dear Colleagues,

The combination of liquid chromatography and mass spectrometry (LC-MS) has been one of the widely used tools of analysis for both chemists and biologists alike for a while now. However, one of the bottlenecks in LC-MS analysis is the lack of available standard methods for assay development (lipids, etc.). This Issue will try to address this by providing a collection of peer-reviewed articles that address LC-MS-related method development for small molecules, both pharmaceutical (drug development, DMPK, toxicology, bio-fluid samples, etc.) and chemical (food science, environmental samples, exposomics etc.) in origin as well as large molecules (e.g., proteins) that are of importance to the biological community and others. The topics of consideration will be method development (involving sample preparation and LC-MS/MS experiments) for analytes related to biomarkers, proteins, antibodies, lipids, nucleic acids, peptides, polymers, peptoids, carbohydrates, natural products, organometallics, small molecules, lichens, drug candidates, pesticides, terpenoids, cannabinoids, forensics, toxicology, and food science for: 1) The discovery of target analytes; 2) Identification and quantitation; 3) Structure elucidation; and 4) Reactivity.

Dr. Ekram Hossain
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • proteomics
  • metabolomics
  • biomarkers
  • antibody
  • method development
  • polymers
  • quantitation
  • lipids
  • structure elucidation
  • MRM
  • triple quad
  • Orbitrap
  • HPLC
  • LC-MS/MS

Published Papers (1 paper)

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Research

14 pages, 3293 KiB  
Article
Determination and Comparison of Short-Chain Fatty Acids in Serum and Colon Content Samples: Alzheimer’s Disease Rat as a Case Study
by Lin-Xiu Guo, Yue Tong, Jue Wang, Guo Yin, Hou-Shuang Huang, Long Zeng, Ping Wang, Jun-Peng Li, Kai-Shun Bi and Tie-Jie Wang
Molecules 2020, 25(23), 5739; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules25235739 - 04 Dec 2020
Cited by 8 | Viewed by 3336
Abstract
Short-chain fatty acids (SCFAs) are the main microbial fermentation products from dietary fibers in the colon, and it has been speculated that they play a key role in keeping healthy in the whole-body. However, differences in SCFAs concentration in the serum and colon [...] Read more.
Short-chain fatty acids (SCFAs) are the main microbial fermentation products from dietary fibers in the colon, and it has been speculated that they play a key role in keeping healthy in the whole-body. However, differences in SCFAs concentration in the serum and colon samples had attracted little attention. In this study, we have optimized the extract and analysis methods for the determination of ten SCFAs in both serum and colon content samples. Methanol and acetonitrile were chosen for extraction of SCFAs from serum and colon content samples, respectively. Biological samples were collected from Alzheimer’s disease rats treated by extract of Schisandra chinensis (Turcz.) Baill (SC-extract) were taken as research objects. The results showed that, the relative peak intensities of SCFAs in the colon content from all groups were quite similar, and the trend was identical in the serum samples. Compared with the values in humans, the ratio of ten SCFAs in rat’s colon was similar, while the percent of acetate in rat’s serum was significantly higher. For therapy of Alzheimer’s disease (AD), SC-extract decreased the concentration of butyrate, 3-Methyvalerate, and caproate in the serum samples towards the trend of normal rats. This study may help our understanding of how SCFAs are transported across colonic epithelium in healthy and diseased organisms. Full article
(This article belongs to the Special Issue LC-MS/MS Method for Metabolomics and Proteomics)
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