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Sampling, Separation and Isolation Techniques of Flavour Compounds

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Flavours and Fragrances".

Deadline for manuscript submissions: closed (30 June 2022) | Viewed by 1944

Special Issue Editor


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Guest Editor
School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3AX, UK
Interests: volatile organic compounds; biological active organic compounds; VOC profiling; VOC as a diagnostic tool in post-harvest biology; food quality and safety and disease; thermal desorption–gas chromatography–mass spectrometry; multivariate data analysis

Special Issue Information

Dear Colleagues,

Flavours provide some of the most enduring sensorial impressions and, in humans, are the result of a complex interaction involving, at least, gustation and ortho- and retro-nasal olfaction. The part of any flavour that is perceived by olfaction is represented by its complement of volatile organic compounds (VOCs). These VOCs are either already present in the original material or produced during preparation (e.g., sulphur compounds in Allium spp. or isothiocyanates in brassicas), cooking or maceration and affected by processes pre- and postharvest.

The profile of VOCs, therefore, contains information about all these processes, and VOC profiling is performed in a wide range of applications from chemical taxonomy (e.g., in Basil), and identification of quantitative trait loci (QTLs) for breeding, to, increasingly, assessment of quality and safety of produce. VOC sampling can be carried out non-destructively and on bulk samples and allow assessments of quality of flavour as such and through correlations of VOCs to shelf-life and other important micronutrients and assessment of safety through changes in flavour that correspond to microbial contamination.

Any analysis of flavours starts with sampling of these often-complex mixtures followed by separation into and isolation of individual compounds.

Therefore, sampling, separation and isolation techniques, critically underpin any studies on, e.g., flavour development, variations of flavour between sources, temporal variations in scent or flavour or changes related to changes in underlying metabolism, physiology or process.

This Special Issue aims to provide an overview and critical evaluation of the methodologies currently in use for sampling of flavour compounds and methods for isolating specific compound.

Dr. Carsten T. Müller
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Volatile organic compounds
  • Extraction methods
  • Field methods
  • Separation methods
  • Isolation methods

Published Papers (1 paper)

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Research

15 pages, 2507 KiB  
Article
Chemical Changes in the Broccoli Volatilome Depending on the Tissue Treatment
by Martyna N. Wieczorek, Piotr Mariusz Pieczywek, Justyna Cybulska, Artur Zdunek and Henryk H. Jeleń
Molecules 2022, 27(2), 500; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27020500 - 14 Jan 2022
Cited by 4 | Viewed by 1583
Abstract
The storage of plant samples as well as sample preparation for extraction have a significant impact on the profile of metabolites, however, these factors are often overlooked during experiments on vegetables or fruit. It was hypothesized that parameters such as sample storage (freezing) [...] Read more.
The storage of plant samples as well as sample preparation for extraction have a significant impact on the profile of metabolites, however, these factors are often overlooked during experiments on vegetables or fruit. It was hypothesized that parameters such as sample storage (freezing) and sample pre-treatment methods, including the comminution technique or applied enzyme inhibition methods, could significantly influence the extracted volatile metabolome. Significant changes were observed in the volatile profile of broccoli florets frozen in liquid nitrogen at −20 °C. Those differences were mostly related to the concentration of nitriles and aldehydes. Confocal microscopy indicated some tissue deterioration in the case of slow freezing (−20 °C), whereas the structure of tissue, frozen in liquid nitrogen, was practically intact. Myrosinase activity assay proved that the enzyme remains active after freezing. No pH deviation was noted after sample storage - this parameter did not influence the activity of enzymes. Tissue fragmentation and enzyme-inhibition techniques applied prior to the extraction influenced both the qualitative and quantitative composition of the volatile metabolome of broccoli. Full article
(This article belongs to the Special Issue Sampling, Separation and Isolation Techniques of Flavour Compounds)
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