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Molecular Imaging and Spectroscopy with Applications to Chemistry

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A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Chemical Biology".

Deadline for manuscript submissions: closed (1 September 2022) | Viewed by 12817

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Special Issue Editor

Dr. Daniel Jirák

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Guest Editor

Department of Diagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic
Interests: Assoc. Prof. Daniel Jirak, Ph.D. has several years of experience with molecular imaging documented in more than 75 publications in (impacted) journals. His research focus is on MR imaging of transplanted cells including imaging of pancreatic islets in animals and humans and on investigating new contrast agents including agents for multimodal imaging

Special Issue Information

Dear Colleagues,

Molecular imaging is a very interesting and important biomedical research discipline that studies processes at cell or even molecular levels. It covers all imaging modalities including techniques used in clinical practice.

Imaging is an important part of understanding many pathological states. For visualization and precise characterization and quantification of specific processes related to cells or molecules, molecular probes are vitally important. This is a clear example of the field of molecular imaging, where medicine and biology need chemistry. Due to this multidisciplinary effort, various contrast agents and probes are available for the imaging of organs, cells, or molecules. Moreover, these probes could be specific, responsive to various stimuli, and can serve as therapeutics.

This Special Issue will cover the applications of various probes based on the observation of various nuclei and contrast agents for different imaging modalities to image molecules or cells in various models including in vivo experiments.

Assoc. Prof. Daniel Jirák
Guest Editor

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Keywords

probe
contrast agent
optical imaging
magnetic resonance imaging and spectroscopy
ultrasonography
positron emission tomography
single-photon computed tomography
computed tomography

Published Papers (5 papers)

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Research

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18 pages, 5475 KiB

Open AccessCommunication

Phosphorus-Containing Polymers as Sensitive Biocompatible Probes for ³¹P Magnetic Resonance

by Lucie Kracíková, Ladislav Androvič, Iveta Potočková, Natalia Ziółkowska, Martin Vít, David Červený, Daniel Jirák and Richard Laga

Molecules 2023, 28(5), 2334; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules28052334 - 02 Mar 2023

Cited by 3 | Viewed by 1909

Abstract

The visualization of organs and tissues using ³¹P magnetic resonance (MR) imaging represents an immense challenge. This is largely due to the lack of sensitive biocompatible probes required to deliver a high-intensity MR signal that can be distinguished from the natural biological background. Synthetic water-soluble phosphorus-containing polymers appear to be suitable materials for this purpose due to their adjustable chain architecture, low toxicity, and favorable pharmacokinetics. In this work, we carried out a controlled synthesis, and compared the MR properties, of several probes consisting of highly hydrophilic phosphopolymers differing in composition, structure, and molecular weight. Based on our phantom experiments, all probes with a molecular weight of ~3–400 kg·mol⁻¹, including linear polymers based on poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC), poly(ethyl ethylenephosphate) (PEEP), and poly[bis(2-(2-(2-methoxyethoxy)ethoxy)ethoxy)]phosphazene (PMEEEP) as well as star-shaped copolymers composed of PMPC arms grafted onto poly(amidoamine) dendrimer (PAMAM-g-PMPC) or cyclotriphosphazene-derived cores (CTP-g-PMPC), were readily detected using a 4.7 T MR scanner. The highest signal-to-noise ratio was achieved by the linear polymers PMPC (210) and PMEEEP (62) followed by the star polymers CTP-g-PMPC (56) and PAMAM-g-PMPC (44). The ³¹P T₁ and T₂ relaxation times for these phosphopolymers were also favorable, ranging between 1078 and 2368 and 30 and 171 ms, respectively. We contend that select phosphopolymers are suitable for use as sensitive ³¹P MR probes for biomedical applications. Full article

(This article belongs to the Special Issue Molecular Imaging and Spectroscopy with Applications to Chemistry)

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13 pages, 3779 KiB

Open AccessArticle

A Preliminary Study of PSMA Fluorescent Probe for Targeted Fluorescence Imaging of Prostate Cancer

by Haoxi Zhou, Yachao Liu, Xiaojun Zhang, Kuang Chen, Yuan Li, Xiaodan Xu and Baixuan Xu

Molecules 2022, 27(9), 2736; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27092736 - 24 Apr 2022

Cited by 1 | Viewed by 1889

Abstract

Purpose: With the increasing detection rate of early prostate cancer (PCa), the proportion of surgical treatment is increasing. Surgery is the most effective treatment for PCa. Precise targeting of tumors during surgery can reduce the incidence of positive surgical margins (PSMs) and preserve the neurovascular bundles (NVBs) as much as possible. The objective of this study was to synthesize a PSMA fluorescent probe (PSMA-Cy5) and verify the targeting specificity of the probe for prostate cancer, thereby providing a theoretical basis for the development of PSMA fluorescent probes for clinical application in the future. Methods: In this study, a novel water-soluble 3H-indocyanine-type bioluminescent dye-Cy5-labeled prostate-specific membrane antigen (PSMA) ligand (PSMA-Cy5) was synthesized by liquid phase synthesis. The PSMA ligand was developed based on the glutamine-urea-lysine (Glu-urea-Lys) structure. The new fluorescent probe was evaluated in vitro and in vivo, and its safety was evaluated. Confocal microscopy was used to observe the binding uptake of PSMA-Cy5 with PSMA (+) LNCaP cells, PSMA (-) PC3 cells and blocked LNCaP cells. In in vivo optical imaging studies, the targeting specificity of PSMA (+) 22Rv1 tumors to probe binding was validated by tail vein injection of PSMA-Cy5. The safety of the PSMA-Cy5 probe was evaluated by histopathological analysis of mouse organs by a single high-dose tail vein injection of PSMA-Cy5. Results: In vitro fluorescence cell uptake experiments showed that the binding of PSMA-Cy5 to LNCaP cells has targeting specificity. PC3 cells and blocked LNCaP cells showed almost no uptake. The results of in vivo optical imaging studies showed that the tumor-to-background ratio in the 22Rv1 group was 3.39 ± 0.47; in the 22Rv1 blocking group it was 0.78 ± 0.15, and in the PC3 group it was 0.94 ± 0.09, consistent with the in vitro results. After a high-dose injection of PSMA-Cy5, there were no abnormalities in the tissues or organs of the mice. The probe showed good safety. Conclusions: PSMA-Cy5 is a probe with good targeting specificity and low toxicity that can accurately visualize tumors in vivo. This study has an important reference value for the development of PSMA fluorescent probes. In the future, it can be applied to precise tumor imaging during radical prostatectomy to reduce the incidence of postoperative PSM. Full article

(This article belongs to the Special Issue Molecular Imaging and Spectroscopy with Applications to Chemistry)

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15 pages, 1002 KiB

Open AccessArticle

In Vitro ³¹P MR Chemical Shifts of In Vivo-Detectable Metabolites at 3T as a Basis Set for a Pilot Evaluation of Skeletal Muscle and Liver ³¹P Spectra with LCModel Software

by Petr Sedivy, Tereza Dusilova, Milan Hajek, Martin Burian, Martin Krššák and Monika Dezortova

Molecules 2021, 26(24), 7571; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules26247571 - 14 Dec 2021

Cited by 3 | Viewed by 2540

Abstract

Most in vivo ³¹P MR studies are realized on 3T MR systems that provide sufficient signal intensity for prominent phosphorus metabolites. The identification of these metabolites in the in vivo spectra is performed by comparing their chemical shifts with the chemical shifts measured in vitro on high-field NMR spectrometers. To approach in vivo conditions at 3T, a set of phantoms with defined metabolite solutions were measured in a 3T whole-body MR system at 7.0 and 7.5 pH, at 37 °C. A free induction decay (FID) sequence with and without ¹H decoupling was used. Chemical shifts were obtained of phosphoenolpyruvate (PEP), phosphatidylcholine (PtdC), phosphocholine (PC), phosphoethanolamine (PE), glycerophosphocholine (GPC), glycerophosphoetanolamine (GPE), uridine diphosphoglucose (UDPG), glucose-6-phosphate (G6P), glucose-1-phosphate (G1P), 2,3-diphosphoglycerate (2,3-DPG), nicotinamide adenine dinucleotide (NADH and NAD⁺), phosphocreatine (PCr), adenosine triphosphate (ATP), adenosine diphosphate (ADP), and inorganic phosphate (Pi). The measured chemical shifts were used to construct a basis set of ³¹P MR spectra for the evaluation of ³¹P in vivo spectra of muscle and the liver using LCModel software (linear combination model). Prior knowledge was successfully employed in the analysis of previously acquired in vivo data. Full article

(This article belongs to the Special Issue Molecular Imaging and Spectroscopy with Applications to Chemistry)

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16 pages, 3281 KiB

Open AccessArticle

Mannan-Based Nanodiagnostic Agents for Targeting Sentinel Lymph Nodes and Tumors

by Markéta Jirátová, Andrea Gálisová, Maria Rabyk, Eva Sticová, Martin Hrubý and Daniel Jirák

Molecules 2021, 26(1), 146; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules26010146 - 31 Dec 2020

Cited by 5 | Viewed by 2107

Abstract

Early detection of metastasis is crucial for successful cancer treatment. Sentinel lymph node (SLN) biopsies are used to detect possible pathways of metastasis spread. We present a unique non-invasive diagnostic alternative to biopsy along with an intraoperative imaging tool for surgery proven on an in vivo animal tumor model. Our approach is based on mannan-based copolymers synergistically targeting: (1) SLNs and macrophage-infiltrated solid tumor areas via the high-affinity DC-SIGN (dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin) receptors and (2) tumors via the enhanced permeability and retention (EPR) effect. The polymer conjugates were modified with the imaging probes for visualization with magnetic resonance (MR) and fluorescence imaging, respectively, and with poly(2-methyl-2-oxazoline) (POX) to lower unwanted accumulation in internal organs and to slow down the biodegradation rate. We demonstrated that these polymer conjugates were successfully accumulated in tumors, SLNs and other lymph nodes. Modification with POX resulted in lower accumulation not only in internal organs, but also in lymph nodes and tumors. Importantly, we have shown that mannan-based polymer carriers are non-toxic and, when applied to an in vivo murine cancer model, and offer promising potential as the versatile imaging agents. Full article

(This article belongs to the Special Issue Molecular Imaging and Spectroscopy with Applications to Chemistry)

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Review

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17 pages, 3660 KiB

Open AccessReview

Radiotracers for Bone Marrow Infection Imaging

by Lars Jødal, Pia Afzelius, Aage Kristian Olsen Alstrup and Svend Borup Jensen

Molecules 2021, 26(11), 3159; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules26113159 - 25 May 2021

Cited by 7 | Viewed by 3515

Abstract

Introduction: Radiotracers are widely used in medical imaging, using techniques of gamma-camera imaging (scintigraphy and SPECT) or positron emission tomography (PET). In bone marrow infection, there is no single routine test available that can detect infection with sufficiently high diagnostic accuracy. Here, we review radiotracers used for imaging of bone marrow infection, also known as osteomyelitis, with a focus on why these molecules are relevant for the task, based on their physiological uptake mechanisms. The review comprises [⁶⁷Ga]Ga-citrate, radiolabelled leukocytes, radiolabelled nanocolloids (bone marrow) and radiolabelled phosphonates (bone structure), and [¹⁸F]FDG as established radiotracers for bone marrow infection imaging. Tracers that are under development or testing for this purpose include [⁶⁸Ga]Ga-citrate, [¹⁸F]FDG, [¹⁸F]FDS and other non-glucose sugar analogues, [¹⁵O]water, [¹¹C]methionine, [¹¹C]donepezil, [^99mTc]Tc-IL-8, [⁶⁸Ga]Ga-Siglec-9, phage-display selected peptides, and the antimicrobial peptide [^99mTc]Tc-UBI_29-41 or [⁶⁸Ga]Ga-NOTA-UBI_29-41. Conclusion: Molecular radiotracers allow studies of physiological processes such as infection. None of the reviewed molecules are ideal for the imaging of infections, whether bone marrow or otherwise, but each can give information about a separate aspect such as physiology or biochemistry. Knowledge of uptake mechanisms, pitfalls, and challenges is useful in both the use and development of medically relevant radioactive tracers. Full article

(This article belongs to the Special Issue Molecular Imaging and Spectroscopy with Applications to Chemistry)

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