Nanotoxicity Analysis

A special issue of Nanomaterials (ISSN 2079-4991). This special issue belongs to the section "Biology and Medicines".

Deadline for manuscript submissions: closed (15 October 2022) | Viewed by 3994

Special Issue Editor


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Guest Editor
Department of Analytical Chemistry, Complutense University of Madrid, 28040 Madrid, Spain
Interests: hybrid nanosystems; mesoporous silica nanoparticles; nanotoxicity, drug delivery; metallic nanoparticles; “omics” techniques; molecular mechanisms of action; cancer; tuberculosis

Special Issue Information

Dear Colleagues,

The increasing use of nanomaterials over a wide range of applications has led to an urgent need to evaluate the impact of these new materials on human health and the environment. To date, the potential toxicity of nanomaterials and their interaction mechanisms with cells and living organisms have not been fully addressed. Examining the toxic effects of nanomaterials at the molecular level can be useful for gaining insights into the mechanisms of toxicity and for identifying potential candidate biomarkers of exposure and response.

This Special Issue is open to original research articles, as well as review papers, and it will focus on the use of analytical and bioanalytical techniques for nanotoxicity investigations, covering everything from sample treatment and analysis using different approaches including omics techniques, to chemometric and bioinformatic tools used for data processing. Articles dealing with the development of novel analytical approaches and/or their use to the study of toxicity mechanisms induced by exposure to nanomaterials, as well as for the identification of potential biomarkers of toxicity or response, will be welcome.


Prof. Dr. Jose L. Luque-Garcia
Guest Editor

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Keywords

  • toxicity
  • apoptosis
  • small molecules
  • sample preparation
  • omics techniques
  • chemometrics
  • biological fluids
  • biomarkers
  • tissues
  • cells
  • molecular pathways

Published Papers (2 papers)

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Research

19 pages, 6337 KiB  
Article
A Multi-Omics Approach to Evaluate the Toxicity Mechanisms Associated with Silver Nanoparticles Exposure
by Guillermo Aragoneses-Cazorla, M. Pilar Buendia-Nacarino, Maria L. Mena and Jose L. Luque-Garcia
Nanomaterials 2022, 12(10), 1762; https://0-doi-org.brum.beds.ac.uk/10.3390/nano12101762 - 22 May 2022
Cited by 7 | Viewed by 1909
Abstract
Silver nanoparticles (AgNPs) are currently used in many different industrial, commercial and health fields, mainly due to their antibacterial properties. Due to this widespread use, humans and the environment are increasingly exposed to these types of nanoparticles, which is the reason why the [...] Read more.
Silver nanoparticles (AgNPs) are currently used in many different industrial, commercial and health fields, mainly due to their antibacterial properties. Due to this widespread use, humans and the environment are increasingly exposed to these types of nanoparticles, which is the reason why the evaluation of the potential toxicity associated with AgNPs is of great importance. Although some of the toxic effects induced by AgNPs have already been shown, the elucidation of more complete mechanisms is yet to be achieved. In this sense, and since the integration of metabolomics and transcriptomics approaches constitutes a very useful strategy, in the present study targeted and untargeted metabolomics and DNA microarrays assays have been combined to evaluate the molecular mechanisms involved in the toxicity induced by 10 nm AgNPs. The results have shown that AgNPs induce the synthesis of glutathione as a cellular defense mechanism to face the oxidative environment, while inducing the depletion of relevant molecules implicated in the synthesis of important antioxidants. In addition, it has been observed that AgNPs completely impair the intracellular energetic metabolism, especially affecting the production of adenosine triphosphate (ATP) and disrupting the tricarboxylic acids cycle. It has been demonstrated that AgNPs exposure also affects the glycolysis pathway. The effect on such pathway differs depending on the step of the cycle, which a significant increase in the levels of glucose as way to counterbalance the depleted levels of ATP. Full article
(This article belongs to the Special Issue Nanotoxicity Analysis)
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14 pages, 1886 KiB  
Article
Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals
by Alexander V. Sirotkin, Monika Radosová, Adam Tarko, Zuzana Fabova, Iris Martín-García and Francisco Alonso
Nanomaterials 2020, 10(9), 1859; https://0-doi-org.brum.beds.ac.uk/10.3390/nano10091859 - 17 Sep 2020
Cited by 9 | Viewed by 2431
Abstract
The application of nanoparticles has experienced a vertiginous growth, but their interaction with food and medicinal plants in organisms, especially in the control of reproduction, remains unresolved. We examined the influence of copper nanoparticles supported on titania (CuNPs/TiO2), plant extracts (buckwheat [...] Read more.
The application of nanoparticles has experienced a vertiginous growth, but their interaction with food and medicinal plants in organisms, especially in the control of reproduction, remains unresolved. We examined the influence of copper nanoparticles supported on titania (CuNPs/TiO2), plant extracts (buckwheat (Fagopyrum esculentum) and vitex (Vitex agnus-castus)), phytochemicals (rutin and apigenin), and their combination with CuNPs/TiO2 on ovarian cell functions, using cultured porcine ovarian granulosa cells. Cell viability, proliferation (PCNA accumulation), apoptosis (accumulation of bax), and hormones release (progesterone, testosterone, and 17β-estradiol) were analyzed by the Trypan blue test, quantitative immunocytochemistry, and ELISA, respectively. CuNPs/TiO2 increased cell viability, proliferation, apoptosis, and testosterone but not progesterone release, and reduced the 17β-estradiol output. Plant extracts and components have similar stimulatory action on ovarian cell functions as CuNPs/TiO2, but abated the majority of the CuNPs/TiO2 effects. This study concludes that (1) CuNPs/TiO2 can directly stimulate ovarian cell functions, promoting ovarian cell proliferation, apoptosis, turnover, viability, and steroid hormones release; (2) the plants buckwheat and vitex, as well as rutin and apigenin, can promote some of these ovarian functions too; and (3) these plant additives mitigate the CuNPs/TiO2’s activity, something that must be considered when applied together. Full article
(This article belongs to the Special Issue Nanotoxicity Analysis)
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