Epidemiology of Methicillin-Resistant Staphylococcus aureus along the Meat Production Chain

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Bacterial Pathogens".

Deadline for manuscript submissions: closed (31 January 2023) | Viewed by 2400

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Laboratory of Hygiene of Foods of Animal Origin – Veterinary Public Health, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
Interests: food microbiology; antimicrobial resistance of foodborne pathogens (especially methicillin-resistant Staphylococcus aureus – MRSA); food hygiene and food safety; veterinary public health

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Guest Editor
Department of Microbiology and Infectious Diseases, School of Veterinary Medicine, Aristotle University of Thessaloniki, Greece, 54124 Thessaloniki, Greece
Interests: Microbiology; Epidemiology; Risk assessment; Staphylococcus spp.; Salmonella

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Guest Editor
Hellenic Agricultural Organisation‐DEMETER, Veterinary Research Institute of Thessaloniki, Thermi, Greece
Interests: microbiology; molecular biology; antibiotic resistance; genomics; host-microbes interactions; immune responses; zoonotic diseases; animal models; epidemiology

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Guest Editor
Laboratory of Animal Health, Food Hygiene and Quality, Department of Agriculture, School of Agriculture, University of Ioannina, Greece, 47100 Kostakioi Artas, Greece
Interests: Food microbiology; food safety; risk assessment; Staphylococcus spp.

Special Issue Information

Dear Colleagues,

Meat is an important nutrition source, and global demand for meat is increasingly growing today. Global meat production is estimated to double by 2050, most of it in developing countries. Increasing livestock production and safe processing and marketing of meat and meat products also represent a big challenge. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important antimicrobial resistant pathogens causing severe life-threatening infections in hospitals and the community. Livestock seems to be a significant reservoir of MRSA; however, a major concern has been extended in the production chain of foods of animal origin. Many studies report close genetic relatedness among isolates of food and human origin; this is an alarming signal to farmers, food industry, and public health agencies regarding the risk of spreading antimicrobial resistance. The prudent use of antimicrobials in livestock is playing a vital role to animal health but also reflects on human health. Understanding the points of exposure to MRSA of livestock, meat processing workers, and the environment is crucial for the development and implementation of best practices to eliminate spreading of MRSA and other resistant bacteria in the community. Therefore, epidemiological studies of MRSA in the meat production chain, from farm, abattoirs, and meat processing plants, could be used as baseline studies for modeling the spread of the pathogen to consumers and the community. These studies could also be applicable to other researchers to perform risk assessment, evaluate the risk for specific products, and inform official authorities for action under the One Health perspective.

Dr. Daniil Sergkelidis
Dr. Theofilos Papadopoulos
Dr. Charalambos Kotzamanidis
Dr. Panagiotis Papadopoulos
Guest Editors

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Keywords

  • methicillin-resistant Staphylococcus aureus
  • antimicrobial resistance
  • livestock
  • meat safety
  • epidemiology
  • One Health

Published Papers (1 paper)

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Research

16 pages, 805 KiB  
Article
Prevalence, Infectious Characteristics and Genetic Diversity of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus (MRSA) in Two Raw-Meat Processing Establishments in Northern Greece
by Dimitrios Komodromos, Charalampos Kotzamanidis, Virginia Giantzi, Styliani Pappa, Anna Papa, Antonios Zdragas, Apostolos Angelidis and Daniel Sergelidis
Pathogens 2022, 11(11), 1370; https://0-doi-org.brum.beds.ac.uk/10.3390/pathogens11111370 - 17 Nov 2022
Cited by 5 | Viewed by 1913
Abstract
In the present study, we investigated the isolation frequency, the genetic diversity, and the infectious characteristics of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) from the incoming meat and the meat products, the environment, and the workers’ nasal cavities, in two meat-processing establishments [...] Read more.
In the present study, we investigated the isolation frequency, the genetic diversity, and the infectious characteristics of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) from the incoming meat and the meat products, the environment, and the workers’ nasal cavities, in two meat-processing establishments in northern Greece. The isolated S. aureus strains were examined for their resistance to antimicrobials, carriage of the mecA and mecC genes, carriage of genes encoding for the production of nine staphylococcal enterotoxins, carriage of the Panton–Valentine Leukocidin and Toxic Shock Syndrome genes, and the ability to form biofilm. The genetic diversity of the isolates was evaluated using Pulsed Field Gel Electrophoresis (PFGE) and spa typing. S. aureus was isolated from 13.8% of the 160 samples examined, while only one sample (0.6%) was contaminated by MRSA carrying the mecA gene. The evaluation of the antimicrobial susceptibility of the isolates revealed low antimicrobial resistance. The higher resistance frequencies were observed for penicillin (68.2%), amoxicillin/clavulanic acid (36.4%) and tetracycline (18.2%), while 31.8% of the isolates were sensitive to all antimicrobials examined. Multidrug resistance was observed in two isolates. None of the isolates carried the mecC or lukF-PV genes, and two isolates (9.1%) harbored the tst gene. Eight isolates (36.4%) carried the seb gene, one carried the sed gene, two (9.1%) carried both the sed and sei genes, and one isolate (4.5%) carried the seb, sed and sei genes. Twenty-one (95.5%) of the isolates showed moderate biofilm production ability, while only one (4.5%) was characterized as a strong biofilm producer. Genotyping of the isolates by PFGE indicates that S. aureus from different meat-processing establishments represent separate genetic populations. Ten different spa types were identified, while no common spa type isolates were detected within the two plants. Overall, our findings emphasize the need for the strict application of good hygienic practices at the plant level to control the spread of S. aureus and MRSA to the community through the end products. Full article
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