Nanotechnology in Engineering and Medicine

A special issue of Pharmaceutics (ISSN 1999-4923). This special issue belongs to the section "Nanomedicine and Nanotechnology".

Deadline for manuscript submissions: closed (30 June 2021) | Viewed by 2932

Special Issue Editor


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Guest Editor
School of Pharmacy, University of Wyoming, Laramie, WY 82071, USA
Interests: molecular mechanisms of pain signaling and nociception; mechanisms of browning of white adipose tissue; mitochondrial quality control in adipose tissues and neurons—in physiology and pathology; role of TRP channels and PIP2-rich lipid microdomains in neurodegenerative and neuromuscular diseases; therapeutic potentials of botulinum neurotoxins; neuropharmaceutics—magnetic nanoparticles for site-specific targeted drug delivery to PNS and CNS

Special Issue Information

Dear Colleagues,

Nanotechnology is an exciting area in science, which has many applications in engineering and medicine. Nanoparticle based systems are emerging as a powerful tool for engineering applications like nanomachining and drug therapy for treating diseases like cancer, neuropathic pain, spot-fat reduction, and cardiovascular diseases. Nanoparticles are also widely used in Polymers are widely employed in TDD approaches for successful delivery to target tissues and to ensure optimal drug delivery for the desired period of time.

The aim of this Special Issue is to cover the cutting-edge research activities and recent research advancements in the area of nanotechnology in engineering and medicine.

Dr. Baskaran Thyagarajan
Guest Editor

Manuscript Submission Information

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Keywords

  • drug delivery
  • nanomaterials
  • pharmaceutical engineering
  • tissue targeting
  • nanomachining
  • drug development and therapeutics

Published Papers (1 paper)

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Research

15 pages, 1617 KiB  
Article
Simple and Fast DNA Based Sensor System for Screening of Small-Molecule Compounds Targeting Eukaryotic Topoisomerase 1
by Kamilla Vandsø Petersen, Asier Selas, Kirstine Mejlstrup Hymøller, Karol Mizielinski, Maria Thorsager, Magnus Stougaard, Concepcion Alonso, Francisco Palacios, Yolanda Pérez-Pertejo, Rosa M. Reguera, Rafael Balaña-Fouce, Birgitta R. Knudsen and Cinzia Tesauro
Pharmaceutics 2021, 13(8), 1255; https://0-doi-org.brum.beds.ac.uk/10.3390/pharmaceutics13081255 - 13 Aug 2021
Cited by 3 | Viewed by 2618
Abstract
Background: Eukaryotic topoisomerase 1 is a potential target of anti-parasitic and anti-cancer drugs. Parasites require topoisomerase 1 activity for survival and, consequently, compounds that inhibit topoisomerase 1 activity may be of interest. All effective topoisomerase 1 drugs with anti-cancer activity act by [...] Read more.
Background: Eukaryotic topoisomerase 1 is a potential target of anti-parasitic and anti-cancer drugs. Parasites require topoisomerase 1 activity for survival and, consequently, compounds that inhibit topoisomerase 1 activity may be of interest. All effective topoisomerase 1 drugs with anti-cancer activity act by inhibiting the ligation reaction of the enzyme. Screening for topoisomerase 1 targeting drugs, therefore, should involve the possibility of dissecting which step of topoisomerase 1 activity is affected. Methods: Here we present a novel DNA-based assay that allows for screening of the effect of small-molecule compounds targeting the binding/cleavage or the ligation steps of topoisomerase 1 catalysis. This novel assay is based on the detection of a rolling circle amplification product generated from a DNA circle resulting from topoisomerase 1 activity. Results: We show that the binding/cleavage and ligation reactions of topoisomerase 1 can be investigated separately in the presented assay termed REEAD (C|L) and demonstrate that the assay can be used to investigate, which of the individual steps of topoisomerase 1 catalysis are affected by small-molecule compounds. The assay is gel-free and the results can be detected by a simple colorimetric readout method using silver-on-gold precipitation rendering large equipment unnecessary. Conclusion: REEAD (C|L) allows for easy and quantitative investigations of topoisomerase 1 targeting compounds and can be performed in non-specialized laboratories. Full article
(This article belongs to the Special Issue Nanotechnology in Engineering and Medicine)
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