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Grapevine–Cooperation and Struggle with Microbial Residents

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A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Ecology".

Deadline for manuscript submissions: closed (31 January 2022) | Viewed by 15110

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Special Issue Editors

Dr. Tomislav Radić

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Guest Editor

Institute for Adriatic Crops and Karst Reclamation, Split, Croatia
Interests: plant-microbe interactions, arbuscular mycorrhizal fungi, fungal endophytes, grapevine viruses

Dr. Katarina Hančević

E-Mail Website
Guest Editor

Institute for Adriatic Crops and Karst Reclamation, Split, Croatia
Interests: plant-microbe interactions, grapevine virology, fungal endophytes

Special Issue Information

Dear Colleagues,

Grapevine (Vitis vinifera L.) is one of the most represented crops worldwide, cultivated in diverse geographic and climate environments and with long history of cultivation and breeding. Accordingly, its economic value has been significant. Viticulture encompasses various interactions of grapevine with its environment and one of the most recent recognized is that with its microbiome. It includes a wide range of hosted microorganisms that may have beneficial, neutral or pathogenic influence to the plant. Due to their widespread occurrence and high diversity as well as developing methodological tools of molecular biology, there is increasing need to evaluate their roles in grapevine health, growth and development.

In this special issue we will focus on the various aspects of the grapevine interactions with its microbial residents, primarily phytopathogens (viruses, fungi, and phytoplasmas) and beneficial microorganisms (mycorrhizal fungi, endophytes). Original scientific articles on their diversity, ecology, influence on grapevine physiology, influence on grapevine health, role in adaptation to climate changes and similar are welcome.

Dr. Tomislav Radić
Dr. Katarina Hančević
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

Vitis vinifera L.
Grapevine-microbes interactions
Grapevine pathogens
Grapevine beneficial microorganisms
Grapevine physiology
Grapevine microbiome diversity

Published Papers (5 papers)

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Research

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21 pages, 7214 KiB

Open AccessArticle

Screening of Croatian Native Grapevine Varieties for Susceptibility to Plasmopara viticola Using Leaf Disc Bioassay, Chlorophyll Fluorescence, and Multispectral Imaging

by Petra Štambuk, Iva Šikuten, Darko Preiner, Ana Nimac, Boris Lazarević, Zvjezdana Marković, Edi Maletić, Jasminka Karoglan Kontić and Ivana Tomaz

Plants 2021, 10(4), 661; https://0-doi-org.brum.beds.ac.uk/10.3390/plants10040661 - 30 Mar 2021

Cited by 8 | Viewed by 3004

Abstract

In the era of sustainable grapevine production, there is a growing demand to define differences between Vitis vinifera varieties in susceptibility to downy mildew. Croatia, as a country with a long tradition of grapevine cultivation, preserves a large number of native grapevine varieties. A leaf disc bioassay has been conducted on 25 of them to define their response to downy mildew, according to the International Organisation of Vine and Wine (OIV) descriptor 452-1, together with the stress response of the leaf discs using chlorophyll fluorescence and multispectral imaging with 11 parameters included. Time points of measurement were as follows: before treatment (T₀), one day post-inoculation (dpi) (T₁), two dpi (T₂), three dpi (T₃), four dpi (T₄), six dpi (T₅), and eight dpi (T₆). Visible changes in form of developed Plasmopara viticola (P. viticola) sporulation were evaluated on the seventh day upon inoculation. Results show that methods applied here distinguish varieties of different responses to downy mildew. Based on the results obtained, a phenotyping model in the absence of the pathogen is proposed, which is required to confirm by conducting more extensive research. Full article

(This article belongs to the Special Issue Grapevine–Cooperation and Struggle with Microbial Residents)

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14 pages, 2223 KiB

Open AccessArticle

Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia

by Katarina Hančević, Pasquale Saldarelli, Mate Čarija, Silvija Černi, Goran Zdunić, Ana Mucalo and Tomislav Radić

Plants 2021, 10(1), 17; https://0-doi-org.brum.beds.ac.uk/10.3390/plants10010017 - 24 Dec 2020

Cited by 11 | Viewed by 2311

Abstract

Sixteen grapevine cultivars from Mediterranean Croatia were surveyed for the presence of 10 of the most economically important grapevine viruses. The presence of Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV), Grapevine leafroll associated virus-1, -2, and -3 (GLRaV-1; GLRaV-2 and GLRaV-3), Grapevine virus A (GVA) and B (GVB), Grapevine fleck virus (GFkV), Grapevine rupestris stem pitting associated virus (GRSPaV), and Grapevine Pinot gris virus (GPGV) were tested by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). All 71 analyzed clones were positive for the presence of one or more viruses. The most abundant one, detected in almost 95% of samples was GLRaV-3. In most of cases it was reported in mixed infections with GVA, GRSPaV, and GPGV. Virus genomes of GLRaV-3 infected vines were further characterized molecularly in order to determine their genetic diversity. Different genomic variants of heat shock 70 protein homologue (HSP70h) were identified by single-strand conformation polymorphism (SSCP) and sequenced. Sequence analysis confirmed their clustering into phylogenetic group I and/or phylogenetic group II. This study emphasizes the wide virus heterogenicity in Mediterranean vines and the predominant presence of GLRaV-3 phylogenetic groups I and II, either individually or in combination. Full article

(This article belongs to the Special Issue Grapevine–Cooperation and Struggle with Microbial Residents)

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24 pages, 2720 KiB

Open AccessArticle

Response of Mycorrhizal ’Touriga Nacional‘ Variety Grapevines to High Temperatures Measured by Calorespirometry and Near-Infrared Spectroscopy

by Amaia Nogales, Hugo Ribeiro, Julio Nogales-Bueno, Lee D. Hansen, Elsa F. Gonçalves, João Lucas Coito, Ana Elisa Rato, Augusto Peixe, Wanda Viegas and Hélia Cardoso

Plants 2020, 9(11), 1499; https://0-doi-org.brum.beds.ac.uk/10.3390/plants9111499 - 05 Nov 2020

Cited by 9 | Viewed by 2272

Abstract

Heat stress negatively affects several physiological and biochemical processes in grapevine plants. In this work, two new methods, calorespirometry, which has been used to determine temperature adaptation in plants, and near-infrared (NIR) spectroscopy, which has been used to determine several grapevine-related traits and to discriminate among varieties, were tested to evaluate grapevine response to high temperatures. ‘Touriga Nacional’ variety grapevines, inoculated or not with Rhizoglomus irregulare or Funneliformis mosseae, were used in this study. Calorespirometric parameters and NIR spectra, as well as other parameters commonly used to assess heat injury in plants, were measured before and after high temperature exposure. Growth rate and substrate carbon conversion efficiency, calculated from calorespirometric measurements, and stomatal conductance, were the most sensitive parameters for discriminating among high temperature responses of control and inoculated grapevines. The results revealed that, although this vine variety can adapt its physiology to temperatures up to 40 °C, inoculation with R. irregulare could additionally help to sustain its growth, especially after heat shocks. Therefore, the combination of calorespirometry together with gas exchange measurements is a promising strategy for screening grapevine heat tolerance under controlled conditions and has high potential to be implemented in initial phases of plant breeding programs. Full article

(This article belongs to the Special Issue Grapevine–Cooperation and Struggle with Microbial Residents)

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Graphical abstract

12 pages, 3330 KiB

Open AccessArticle

Development of a Full-Length Infectious cDNA Clone of the Grapevine Berry Inner Necrosis Virus

by Xudong Fan, Zunping Zhang, Fang Ren, Guojun Hu, Chen Li, Baodong Zhang and Yafeng Dong

Plants 2020, 9(10), 1340; https://0-doi-org.brum.beds.ac.uk/10.3390/plants9101340 - 11 Oct 2020

Cited by 7 | Viewed by 2461

Abstract

Grapevine berry inner necrosis virus (GINV) belongs to the genus Trichovirus in the family Betaflexiviridae. The GINV isolate LN_BETA_RS was obtained from a “Beta” grapevine (Vitis riparia × Vitis labrusca) exhibiting chlorotic mottling and ring spot in Xingcheng, Liaoning Province, China. To verify the correlation between GINV and grapevine chlorotic mottling and ring spot disease, we constructed an infectious cDNA clone of GINV isolate LN_BETA_RS using the seamless assembly approach. Applied treatments of agroinfiltration infectious cDNA confirmed systemic GINV infection of the Nicotianaoccidentalis 37B by reverse transcription polymerase chain reaction (RT-PCR) and transmission electron microscopy, exhibiting chlorotic mottling symptoms on leaves. Infectious cDNA was also transmitted to new healthy N. occidentalis plants through rub-inoculation. Moreover, the cDNA clone was agroinfiltrated into “Beta” and “Thompson Seedless” grapevine plantlets, and the inoculated grapevines exhibited leaf chlorotic mottling and ringspot during the two years of observation. GINV-inoculated “Beta” grapevines had serious leaf chlorotic mottling and ringspot symptoms on the whole plant, while relatively few symptoms were observed on the leaves of agroinoculated “Thompson Seedless” grapevines in early spring and only weak ring spot gradually appeared later in the top young leaves. Our experiments fulfilled Koch’s postulates and revealed the causative role of GINV in grapevine chlorotic mottling and ring spot disease. Full article

(This article belongs to the Special Issue Grapevine–Cooperation and Struggle with Microbial Residents)

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Review

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18 pages, 2406 KiB

Open AccessReview

Grapevine Virology in the Third-Generation Sequencing Era: From Virus Detection to Viral Epitranscriptomics

by Vahid Jalali Javaran, Peter Moffett, Pierre Lemoyne, Dong Xu, Charith Raj Adkar-Purushothama and Mamadou Lamine Fall

Plants 2021, 10(11), 2355; https://0-doi-org.brum.beds.ac.uk/10.3390/plants10112355 - 31 Oct 2021

Cited by 12 | Viewed by 4262

Abstract

Among all economically important plant species in the world, grapevine (Vitis vinifera L.) is the most cultivated fruit plant. It has a significant impact on the economies of many countries through wine and fresh and dried fruit production. In recent years, the grape and wine industry has been facing outbreaks of known and emerging viral diseases across the world. Although high-throughput sequencing (HTS) has been used extensively in grapevine virology, the application and potential of third-generation sequencing have not been explored in understanding grapevine viruses and their impact on the grapevine. Nanopore sequencing, a third-generation technology, can be used for the direct sequencing of both RNA and DNA with minimal infrastructure. Compared to other HTS methods, the MinION nanopore platform is faster and more cost-effective and allows for long-read sequencing. Due to the size of the MinION device, it can be easily carried for field viral disease surveillance. This review article discusses grapevine viruses, the principle of third-generation sequencing platforms, and the application of nanopore sequencing technology in grapevine virus detection, virus–plant interactions, as well as the characterization of viral RNA modifications. Full article

(This article belongs to the Special Issue Grapevine–Cooperation and Struggle with Microbial Residents)

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