Recent Research on Electrochemical Bioassays

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Chemical Processes and Systems".

Deadline for manuscript submissions: closed (10 March 2022) | Viewed by 9087

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Guest Editor
Department of Physical Chemistry University of Bucharest, 030018-Bucharest, Romania
Interests: electrochemical biosensors; surface plasmon resonance biosensors; homogenous catalysis; reaction kinetics; self-assembled monolayers; surface coatings
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Special Issue Information

Dear Colleagues,

Electrochemical biosensors have emerged as powerful detection tools for various targets, experiencing a tremendous boost since they first were developed back in the mid-1950s. Nowadays, electrochemical biosensors are ubiquitously involved in food safety assessment, environmental monitoring, drug discovery, forensics, and medical diagnosis. As the sensitivity of biosensors is closely related to the transducer characteristics, and the selectivity and specificity are intimately connected to the type of recognition elements, novel innovative materials, coating strategies and assay formats are continuously required for the achievement of high-performance bio-sensing platforms. In this context, the use of nanocomposite (NC) materials has a significant impact on the analytical performance of the transducer, increasing the conducting and catalytic properties or enhancing the accessibility of the analyte to the recognition elements. These approaches allow the fabrication of easy-to-use and cost-effective analytical devices, stressing on their potential in biological samples detection. The combination between nanostructured biosensors and microfluidic systems provides powerful analytical platforms for point of care (POC) applications, especially for the fast detection of pathogens such as viruses and bacteria.

This Special Issue, entitled “Recent Research on Electrochemical Bioassays,” is dedicated to the latest achievements in the field of electrochemical biosensors for pathogen detection. Topics include, but are not limited to:

  • Materials that are used in the transducer layer for signal amplification: carbonaceous materials (carbon nanotubes, graphene, etc), metal nanoparticles and nanowires
  • Innovative immobilization techniques for DNA, RNA, peptide, enzyme, and antibody probes
  • Affinity and enzymatic assay formats, labelled or non-labelled approaches
  • Miniaturized biosensing devices aiming POC applications

Dr. Mihaela Puiu
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosensors
  • nanocomposite materials
  • pathogen detection
  • point-of-care testing
  • microfluidics

Published Papers (3 papers)

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Research

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16 pages, 1526 KiB  
Article
Fast Electrochemical Measurement of Laccase Activity for Monitoring Grapes’ Infection with Botrytis cinerea
by Andreea Catalina Lulea, Robert Ruginescu, Roberta Maria Banciu, Catalina Pantazi, Elena Brinduse, Marian Ion, Silvia Quintela, Edurne Elejalde, Laura Fernández-de-Castro, Maria Carmen Villarán, Zuria Ruiz-de-Vergara, Cristobal Ruíz, Petru Epure, Cristina Purcarea and Alina Vasilescu
Processes 2022, 10(3), 575; https://0-doi-org.brum.beds.ac.uk/10.3390/pr10030575 - 15 Mar 2022
Cited by 3 | Viewed by 2508
Abstract
Grapes’ infection with the fungi Botrytis cinerea is one of the major causes of economic loss in the winemaking sector worldwide. The laccase activity of grapes is considered an appropriate indicator of this type of fungal infection, and enzymatic activity higher than 3 [...] Read more.
Grapes’ infection with the fungi Botrytis cinerea is one of the major causes of economic loss in the winemaking sector worldwide. The laccase activity of grapes is considered an appropriate indicator of this type of fungal infection, and enzymatic activity higher than 3 U/mL indicates a high risk of irreversibly damaged grape must due to enzymatic browning. This work describes a fast test for the measurement of laccase activity based on a dual optical and electrochemical detection method. A paper sensor impregnated with the enzymatic substrate dye 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) provides a semi-quantitative optical measurement. While the paper sensor can be used independently, when combined with a screen-printed electrode and amperometry measurements, it enables the quantitative detection of laccase activities down to 0.4 U/mL in only 5 min. The method was applied for monitoring the artificial infection of white, rosé, and red grapes with different strains of Botrytis cinerea. The results were confirmed by parallel analysis using the spectrophotometric method of laccase activity determination based on syringaldazine. The influence of the fungal strain and type of grape on laccase activity levels is reported. The demonstrated robustness, simplicity, and versatility of the developed method make it ideal for application on-site in the vineyard or at grape processing points. Full article
(This article belongs to the Special Issue Recent Research on Electrochemical Bioassays)
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13 pages, 2736 KiB  
Article
Removal of Heavy Metals from Wastewater Using Novel Polydopamine-Modified CNTs-Based Composite Membranes
by Faizah Altaf, Shakeel Ahmed, Muhammad Usman, Tahira Batool, Jaweria Shamshad, Patrizia Bocchetta and Rida Batool
Processes 2021, 9(12), 2120; https://0-doi-org.brum.beds.ac.uk/10.3390/pr9122120 - 25 Nov 2021
Cited by 3 | Viewed by 2059
Abstract
The presence of major heavy metals including Pb2+, Cu2+, Co2+, Ni2+, Hg2+, Cr6+, Cd2+, and Zn2+ in water is of great concern because they cannot degrade or be [...] Read more.
The presence of major heavy metals including Pb2+, Cu2+, Co2+, Ni2+, Hg2+, Cr6+, Cd2+, and Zn2+ in water is of great concern because they cannot degrade or be destroyed. They are toxic even at very low concentrations. Therefore, it is necessary to remove such toxicants from water. In the current study, polydopamine carbon nanotubes (PD-CNTs) and polysulfone (PS) composite membranes were prepared. The structural and morphological features of the prepared PDCN composite membranes were studied using FTIR, XRD, SEM, and EDS. The potential application of PDCNs for heavy metal removal was studied for the removal of Pb2+, Cr6+, and Cd2+ from wastewater. The maximum removal efficiency of 96.1% was obtained for Cr6+ at 2.6 pH using a composite membrane containing 1.0% PD-CNTs. The removal efficiencies decreased by 64.1 and 73.4, respectively, by enhancing the pressure from 0.50 up to 0.85 MPa. Under the same circumstances, the percentages of Pb+2 removal at 0.49 bar by the PDCNS membranes containing 0.5% and 1.0% PD-CNT were 70 and 90.3, respectively, and decreased to 54.3 and 57.0, respectively, upon increasing the pressure to 0.85 MPa. The results showed that PDCNS membranes have immense potential for the removal of heavy metals from water. Full article
(This article belongs to the Special Issue Recent Research on Electrochemical Bioassays)
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Review

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29 pages, 1452 KiB  
Review
Recent Developments in Voltammetric Analysis of Pharmaceuticals Using Disposable Pencil Graphite Electrodes
by Iulia Gabriela David, Mihaela Buleandra, Dana Elena Popa, Mihaela Carmen Cheregi, Vasile David, Emilia Elena Iorgulescu and Georgiana Oana Tartareanu
Processes 2022, 10(3), 472; https://0-doi-org.brum.beds.ac.uk/10.3390/pr10030472 - 26 Feb 2022
Cited by 23 | Viewed by 3743
Abstract
The even growing production of both well-known and new derivatives with pharmaceutical action involves the need for developing facile and reliable methods for the analysis of these compounds. Among the widely used instrumental techniques, the electrochemical ones are probably the simplest and the [...] Read more.
The even growing production of both well-known and new derivatives with pharmaceutical action involves the need for developing facile and reliable methods for the analysis of these compounds. Among the widely used instrumental techniques, the electrochemical ones are probably the simplest and the most rapid, also having good performance characteristics. However, the key tool in electroanalysis is the working electrode. Due to the inherent electrochemical and economic advantages of the pencil graphite electrode (PGE), the interest in its applicability in the analysis of different analytes has continuously increased in recent years. Thus, this paper aims to review the scientific reports published in the last 10 years on the use of the disposable eco- and user-friendly PGEs in the electroanalysis of compounds of pharmaceutical importance in different matrices. The PGE characteristics and designs (bare or modified with various types of materials), along with their applications and performance parameters (e.g., linear range, limit of detection, and reproducibility), will be discussed, and their advantages and limitations will be critically emphasized. Full article
(This article belongs to the Special Issue Recent Research on Electrochemical Bioassays)
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