Advances of Food Safety and Toxicology in Food Processes and Engineering

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Food Process Engineering".

Deadline for manuscript submissions: closed (25 March 2024) | Viewed by 20942

Special Issue Editors

Department of Genetics, University of Córdoba, 14071 Córdoba, Spain
Interests: biosafety; molecular biology; food science; molecular biological techniques; biotechnology; genetic toxicology; food safety; epigenetics; toxicology; functional foods
Agri-Food Laboratory, (CAPDER), Avda Menéndez Pidal, s/n, 14080 Córdoba, Spain
Interests: near-infrared spectroscopy (NIR); sensors; quality; food science; health; food processing; ready-to-eat vegetables
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue on “Food Safety and Toxicology” seeks high quality works focusing on advances arising from the search for processed and natural new products for human and animal nutrition. Society is becoming aware that the future of countries will depend on how they eat, with emphasis being placed on safe and low ecological impact processes to obtain foods, functional foods, and food additives as well as bioassays to evaluate such as safety. In parallel, new models for assessing toxicology, genotoxicology, chemoprevention, and aging on animal and human feeding need to be validated for the new processed and natural products.

Topics include, but are not limited to:

  • New natural products for human and animal nutrition
  • Processed products for human and nutrition
  • Ready-to-eat foods
  • Postharvest technology
  • Food quality technology
  • New biological models for toxicology evaluation

Prof. Dr. Ángeles Alonso-Moraga
Dr. Mercedes Del Río Celestino
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • functional foods
  • genotoxicology
  • food additives
  • chemoprevention
  • toxicology
  • aging
  • epigenetics
  • nanoparticles

Published Papers (8 papers)

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Research

Jump to: Review

14 pages, 2374 KiB  
Article
What about the Arsenic? Health Risk Assessment in Canned Tuna Commercialized in Northern Spain
by Carlos Valiente-Diaz, Alejandra del Valle, Eva Garcia-Vazquez, Gonzalo Machado-Schiaffino and Alba Ardura
Processes 2023, 11(3), 824; https://0-doi-org.brum.beds.ac.uk/10.3390/pr11030824 - 09 Mar 2023
Cited by 1 | Viewed by 2384
Abstract
The incorrect labeling, as well as the bioaccumulation of heavy metals in seafood, represent a recurring problem worldwide, not only for natural resources but also for the consumers’ health. Heavy metals can be accumulated through the food chain and transferred to the final [...] Read more.
The incorrect labeling, as well as the bioaccumulation of heavy metals in seafood, represent a recurring problem worldwide, not only for natural resources but also for the consumers’ health. Heavy metals can be accumulated through the food chain and transferred to the final human consumer. Despite its toxicology, arsenic does not have a concentration limit on food, unlike other heavy metals like cadmium, mercury, and lead. Tuna species, with a worldwide distribution and high per capita consumption, represent a well-known toxicological issue caused by heavy metals. In this context, 80 samples of canned tuna were analyzed to check if the information contained in the label was correct and complete. Genetic identification was made by sequencing a fragment of 16S rDNA from 80 samples. For the heavy metal quantification, only those samples with the complete FAO fishing area information on the label were analyzed. Only 29 out of 80 samples presented enough information on the labels for the analysis. Some of the canned tuna commercialized in Spanish markets surpassed the safety standard levels established by the Joint FAO/WHO Expert Committee on Food Activities (JECFA) under the consumption rates of 300 g and 482 g per week. However, the carcinogenic risk (CRlim) for arsenic in all cans and all scenarios was higher than the safety levels. Full article
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8 pages, 2703 KiB  
Article
Evaluation of Porcine and Aspergillus oryzae α-Amylases as Possible Model for the Human Enzyme
by Mauro Marengo, Davide Pezzilli, Eleonora Gianquinto, Alex Fissore, Simonetta Oliaro-Bosso, Barbara Sgorbini, Francesca Spyrakis and Salvatore Adinolfi
Processes 2022, 10(4), 780; https://0-doi-org.brum.beds.ac.uk/10.3390/pr10040780 - 15 Apr 2022
Cited by 5 | Viewed by 2222
Abstract
α-amylases are ubiquitous enzymes belonging to the glycosyl hydrolase (GH13) family, whose members share a high degree of sequence identity, even between distant organisms. To understand the determinants of catalytic activity of α-amylases throughout evolution, and to investigate the use of homologous enzymes [...] Read more.
α-amylases are ubiquitous enzymes belonging to the glycosyl hydrolase (GH13) family, whose members share a high degree of sequence identity, even between distant organisms. To understand the determinants of catalytic activity of α-amylases throughout evolution, and to investigate the use of homologous enzymes as a model for the human one, we compared human salivary α-amylase, Aspergillus oryzae α-amylase and pancreatic porcine α-amylase, using a combination of in vitro and in silico approaches. Enzyme sequences were aligned, and structures superposed, whereas kinetics were spectroscopically studied by using commercial synthetic substrates. These three enzymes show strikingly different activities, specifically mediated by different ions, despite relevant structural homology. Our study confirms that the function of α-amylases throughout evolution has considerably diverged, although key structural determinants, such as the catalytic triad and the calcium-binding pocket, have been retained. These functional differences need to be carefully considered when α-amylases, from different organisms, are used as a model for the human enzymes. In this frame, particular focus is needed for the setup of proper experimental conditions. Full article
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20 pages, 1527 KiB  
Article
Toxicological and Nutraceutical Screening Assays of Some Artificial Sweeteners
by Marcos Mateo-Fernández, Miguel Josué González-Jiménez, Mercedes Del Río Celestino, Rafel Font, Ángeles Alonso-Moraga and Tania Merinas-Amo
Processes 2022, 10(2), 410; https://0-doi-org.brum.beds.ac.uk/10.3390/pr10020410 - 20 Feb 2022
Cited by 5 | Viewed by 2817
Abstract
Artificial sweeteners are food additives worldwide used instead of fructose or glucose in many diet beverages. Furthermore, diet beverages intake has been increasing every year. Thus, some food agencies should regulate it based on toxicological studies. Debates and controversial results are demonstrated, and [...] Read more.
Artificial sweeteners are food additives worldwide used instead of fructose or glucose in many diet beverages. Furthermore, diet beverages intake has been increasing every year. Thus, some food agencies should regulate it based on toxicological studies. Debates and controversial results are demonstrated, and authority can revise its decision on the basis of new data reporting toxicological effects since cyclamate has been forbidden in some countries. Therefore, the aim of this study was to report new data about the toxicity of acesulfame-k, aspartame, and cyclamate, which are useful for authority agencies, determining the toxic potential and nutraceutical capabilities of these compounds. The toxicity, antitoxicity, genotoxicity, antigenotoxicity, and life expectancy assays were carried out in Drosophila as an in vivo model. In addition, in vitro HL-60 line cell was used to evaluate the chemopreventive activity determining the cytotoxic effect and the capability of producing DNA damage due to internucleosomal fragmentation or DNA strand breaks. Furthermore, the methylated status of these cancer cells treated with the tested compounds was assayed as a cancer therapy. Our results demonstrated that all tested compounds were neither toxic nor genotoxic, whereas these compounds resulted in antigenotoxic and cytotoxic substances, except for cyclamate. Aspartame showed antitoxic effects in Drosophila. All tested compounds decreased the quality of life of this in vivo organism model. Acesulfame-k, aspartame, and cyclamate induced DNA damage in the HL-60 cell line in the comet assay, and acesulfame-k generally increased the methylation status. In conclusion, all tested artificial sweeteners were safe compounds at assayed concentrations since toxicity and genotoxicity were not significantly induced in flies. Moreover, Aspartame and Cyclamate showed protective activity against a genotoxin in Drosophila Regarding nutraceutical potential, acesulfame-k and aspartame could be demonstrated to be chemopreventive due to the cytotoxicity activity shown by these compounds. According to DNA fragmentation and comet assays, a necrotic way could be the main mechanism of death cells induced by acesulfame-k and aspartame. Finally, Acesulfame-K hypermethylated repetitive elements, which are hypomethylated in cancer cells resulting in a benefit to humans. Full article
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18 pages, 2178 KiB  
Article
Impact of Seasonal Variation and Processing Methods on the Cassava-Derived Dietary Cyanide Poisoning, Nutritional Status, and Konzo Appearance in South-Kivu, Eastern D.R. Congo
by Marius Baguma, Christiane Migabo, Fabrice Nzabara, Wany Linda Sami, Christian Manegabe Akili, Samuel Makali Lwamushi, Jules Mufungizi Bisimwa, Aimé Nkemba, Pacifique Chirhalwirwa, Ghislain Balemba Maheshe, Jean-Michel Rigo and Joëlle Nsimire Chabwine
Processes 2022, 10(2), 337; https://0-doi-org.brum.beds.ac.uk/10.3390/pr10020337 - 10 Feb 2022
Cited by 2 | Viewed by 1752
Abstract
This study aimed at evaluating the impact of seasons on the nutritional status and on dietary cassava-related cyanide exposure in Burhinyi and Idjwi, two areas in the eastern Democratic Republic of the Congo, witnessing similarly high cassava-derived cyanide poisoning but differently affected by [...] Read more.
This study aimed at evaluating the impact of seasons on the nutritional status and on dietary cassava-related cyanide exposure in Burhinyi and Idjwi, two areas in the eastern Democratic Republic of the Congo, witnessing similarly high cassava-derived cyanide poisoning but differently affected by konzo and malnutrition. Cyanide content in cassava roots and flour, and urinary thiocyanate levels (uSCN) of 54 subjects (40 from Burhinyi and 14 from Idjwi, aged 28.7 (12.1) years, 63% women) were measured during the rainy season (RS) and dry season (DS), using picrate paper kits A and D1. Local processing methods proved to be efficient in removing cyanogenic compounds in fresh cassava roots during the RS. However, the cyanide content in flour samples significantly increased during DS, with ~50% of samples containing unsafe levels (>10 ppm) of cyanide content. Strikingly, the uSCN (µmol/L), from being comparably high in RS (~172.0), slightly decreased during DS in Burhinyi (~103.2; p = 0,3547), but not in Idjwi (~172; p = 0,1113). Furthermore, serum proteins and albumin levels significantly decreased during the DS, witnessing a worsening of nutritional status, in Burhinyi but not in Idjwi. The consumption of bitter cassava roots (OR = 5.43, p = 0.0144) and skipping heap fermentation (OR = 16.67, p = 0.0021) were independently associated with very high uSCN levels during the DS. Thus, restoring the traditional processing methods, and complying with them in either season should ensure the safe consumption of cassava. Full article
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21 pages, 1988 KiB  
Article
Safety and Protective Activities of Manufactured Alcohol-Free Beers
by Tania Merinas-Amo, Mercedes Del Río Celestino, Rafael Font and Ángeles Alonso-Moraga
Processes 2022, 10(2), 331; https://0-doi-org.brum.beds.ac.uk/10.3390/pr10020331 - 09 Feb 2022
Cited by 4 | Viewed by 1400
Abstract
Nowadays, a general interest in improving health in order to achieve better conditions of life is increasing. Diet is a complex factor affecting health conditions. We analysed the biological activities of three types of alcohol-free lager beer (a blond, a pale-blond and a [...] Read more.
Nowadays, a general interest in improving health in order to achieve better conditions of life is increasing. Diet is a complex factor affecting health conditions. We analysed the biological activities of three types of alcohol-free lager beer (a blond, a pale-blond and a stout beer) as well as epicatechin gallate (ECG) as one of their most abundant phenols with the aim of revealing them as nutraceuticals. For that purpose, we carried out safety and protective assays of the tested substances in the well-known Drosophila melanogaster animal model. Moreover, chemoprevention studies on human leukaemia cells (HL-60) in an in vitro model were carried out to evaluate the viability and genomic damage potential of the studied compounds on the tumour cell line. Results suggest the safety properties of all compounds, although pale-blond and stout beer only showed genotoxic activity at the lowest concentrations assayed. Moreover, alcohol-free beers and phenols were able to protect against H2O2 oxidative damage as well as to induce an increase in longevity with an improvement of the quality of life in the in vivo animal model assayed. Promising results were obtained with the alcohol-free beers and ECG in the in vitro assays with human leukaemia cells as they inhibited the tumour cells’ growth, induced DNA damage and modified the methylation status of such a cancer cell line. To sum up, alcohol-free beers should be of interest not only because of their reduced calories and isotonic properties but because they can be recognised as nutraceutical substances. Full article
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13 pages, 2088 KiB  
Article
Lentinan Impairs the Early Development of Zebrafish Embryos, Possibly by Disrupting Glucose and Lipid Metabolism
by Wenyu Miao, Lingling He, Tao Zhang and Chunqi Li
Processes 2022, 10(1), 120; https://0-doi-org.brum.beds.ac.uk/10.3390/pr10010120 - 07 Jan 2022
Cited by 6 | Viewed by 1851
Abstract
LNT is the major biologically active substance extracted from Lentinus edodes (L. edodes). Although functional and pharmacological studies have demonstrated that LNT has multiple benefits for animals and humans, the safety assessment is far from sufficient. To evaluate the potential safety [...] Read more.
LNT is the major biologically active substance extracted from Lentinus edodes (L. edodes). Although functional and pharmacological studies have demonstrated that LNT has multiple benefits for animals and humans, the safety assessment is far from sufficient. To evaluate the potential safety risk, larval zebrafish were continuously exposed to varying concentrations of LNT for 120 h. The 96 h LC50 of LNT was determined to be 1228 μg/mL, and morphological defects including short body length, reduced eye and swim bladder sizes and yolk sac edema were observed. In addition, LNT exposure significantly reduced the blood flow velocity and locomotor activity of larval zebrafish. The biochemical parameters were also affected, showing reduced glucose, triglyceride and cholesterol levels in zebrafish larvae after being exposed to LNT. Correspondingly, the genes involved in glucose and lipid metabolism were disrupted. In conclusion, the present study demonstrates the adverse potential of high concentrations of LNT on the development of zebrafish larvae in the early life stage. Full article
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9 pages, 2537 KiB  
Article
Hepatic mRNA Expression Levels of the Oncogenes Alpha-Fetoprotein and Osteopontin as Diagnostics for Liver Cancer in a Murine Model of Diet-Induced Non-Alcoholic Steatohepatitis
by Anthony J. Pellicano, Stephanie Mullan, Itzhak D. Goldberg and Prakash Narayan
Processes 2021, 9(9), 1516; https://0-doi-org.brum.beds.ac.uk/10.3390/pr9091516 - 26 Aug 2021
Viewed by 1427
Abstract
Non-alcoholic steatohepatitis (NASH) is associated with an increased risk of hepatocellular carcinoma (HCC). Expression levels of hepatic oncogenes, alpha-fetoprotein (afp) and osteopontin (opn)/secreted phosphoprotein 1 (spp1), were investigated using a model of diet-induced NASH. Mice were randomized [...] Read more.
Non-alcoholic steatohepatitis (NASH) is associated with an increased risk of hepatocellular carcinoma (HCC). Expression levels of hepatic oncogenes, alpha-fetoprotein (afp) and osteopontin (opn)/secreted phosphoprotein 1 (spp1), were investigated using a model of diet-induced NASH. Mice were randomized to a standard diet or a fast-food diet (FFD) for 17 months. Livers from the FFD cohort exhibited hallmark characteristics of NASH with liver fibrosis, with a subset of animals exhibiting HCC. Expression levels of hepatic afp and opn/spp1 were elevated ~2.5 and ~5-fold, respectively, in the FFD cohort. Hepatic opn/spp1 exhibited a direct (r = 0.65) and significant (p < 0.01) correlation with liver hydroxyproline content. Receiver operating characteristic (ROC) curve analysis for hepatic afp, as a diagnostic for HCC, returned an area under (AU) ROC 0.84, a sensitivity of 87.5%, a specificity of 77% and a threshold of >1.05-fold change in mRNA level. The use of hepatic opn/ssp1 as a diagnostic for HCC returned an AUROC 0.88, a sensitivity of 83.3%, a specificity of 86.7% and a threshold of >2.4-fold change in mRNA level. These data point to a transformation of NASH to an oncotype with hepatic oncogene levels as a diagnostic for NASH. Full article
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Review

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14 pages, 1491 KiB  
Review
Pathways of Mycotoxin Occurrence in Meat Products: A Review
by Jelka Pleadin, Tina Lešić, Dragan Milićević, Ksenija Markov, Bojan Šarkanj, Nada Vahčić, Ivana Kmetič and Manuela Zadravec
Processes 2021, 9(12), 2122; https://0-doi-org.brum.beds.ac.uk/10.3390/pr9122122 - 25 Nov 2021
Cited by 11 | Viewed by 5585
Abstract
Documented cases of mycotoxin occurrence in meat products call for further research into potential contamination sources, especially given an ever more increasing consumption of these nutritionally rich products. These foodstuffs can be contaminated with mycotoxins through three pathways: contaminated spices and other raw [...] Read more.
Documented cases of mycotoxin occurrence in meat products call for further research into potential contamination sources, especially given an ever more increasing consumption of these nutritionally rich products. These foodstuffs can be contaminated with mycotoxins through three pathways: contaminated spices and other raw materials, mycotoxin-producing moulds present on the surface of dry-cured meat products, and carry-over effect from farm animals exposed to contaminated feed. In order to establish meat products’ mycotoxin contamination more precisely, the concentrations of all mycotoxins of relevance for these products should be determined. This manuscript reviews data on major mycotoxins present in different types of meat products, and discusses the contamination pathways, contamination levels and control & preventative measures. Full article
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