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Application of Smart Fluorescent Proteins for Biosensing

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: closed (25 December 2021) | Viewed by 2711

Special Issue Editors

Department of Biomolecular Science and Engineering, The Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan
Interests: bioimaging, biosensors; protein engineering; molecular recognition
Graduate School of Biostudies; Kyoto University
Interests: proteins; molecular biology; gene expression; Protein Purification; cell biology; PCR; protein expression; electrophoresis; cell culture; gel electrophoresis; western blot analysis; SDS-PAGE

Special Issue Information

Dear Colleagues,

Nowadays, fluorescent protein is a convenient and promising probe to sense and visualize the physiological phenomena in life science. Their genetic encodability is advantageous to the application on measurements of living cells, which requires less-invasiveness and retainability on the probes. After the first report of recombinant gene expression of green fluorescent protein in live cells in 1994, a variety of fluorescent proteins with a wide wavelength range covering from near-ultraviolet to near-infrared have been created through devotion by many researchers. Even now, new fluorescent proteins with improved properties to satisfy advanced requirements are continuously reported. Improvement is also oriented to the direction other than detection and fluorescent proteins available for optogenetic manipulation that are emerging. Furthermore, recently developing fluorescent proteins that incorporate endogenous external cofactors as chromophores are expanding the capability of the fluorescent protein.

This Special Issue encourages authors to submit cutting edge applications of such recent “smart fluorescent proteins”. Applications away from conventional sensing and imaging such as optogenetic tools or memory devices are also welcome. Improvement of analytical methods related to smart fluorescent proteins will also be included. Original manuscripts as well as reviews of the current state of the art are welcome for submission.

Topics to be considered:

  • fluorescent protein
  • fluorescent probe
  • fluorescent sensor
  • fluorescent indicator
  • diagnostic sensor
  • environmental sensor
  • two-photon excitation microscopy
  • total internal reflection microscopy
  • super-resolution microscopy
  • fluorescence lifetime imaging microscopy
  • photoacoustic imaging
  • correlative light and electron microscopy
  • photoactuator
  • photomanipulator
  • photosensitizer
  • memory device

Dr. Tomoki Matsuda
Prof. Hiromi Imamura
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (1 paper)

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Research

11 pages, 3156 KiB  
Communication
Generation of Fluorescent Bacteria with the Genes Coding for Lumazine Protein and Riboflavin Biosynthesis
by Sunjoo Lim, Eugeney Oh, Miae Choi, Euiho Lee and Chan-Yong Lee
Sensors 2021, 21(13), 4506; https://0-doi-org.brum.beds.ac.uk/10.3390/s21134506 - 30 Jun 2021
Cited by 1 | Viewed by 2227
Abstract
Lumazine protein is a member of the riboflavin synthase superfamily and the intense fluorescence is caused by non-covalently bound to 6,7-dimethyl 8-ribityllumazine. The pRFN4 plasmid, which contains the riboflavin synthesis genes from Bacillus subtilis, was originally designed for overproduction of the fluorescent [...] Read more.
Lumazine protein is a member of the riboflavin synthase superfamily and the intense fluorescence is caused by non-covalently bound to 6,7-dimethyl 8-ribityllumazine. The pRFN4 plasmid, which contains the riboflavin synthesis genes from Bacillus subtilis, was originally designed for overproduction of the fluorescent ligand of 6,7-dimethyl 8-ribityllumazine. To provide the basis for a biosensor based on the lux gene from bioluminescent bacteria of Photobacterium leiognathi, the gene coding for N-terminal domain half of the lumazine protein extending to amino acid 112 (N-LumP) and the gene for whole lumazine protein (W-LumP) from P. leiognathi were introduced by polymerase chain reaction (PCR) and ligated into pRFN4 vector, to construct the recombinant plasmids of N-lumP-pRFN4 and W-lumP-pRFN4 as well as their modified plasmids by insertion of the lux promoter. The expression of the genes in the recombinant plasmids was checked in various Escherichia coli strains, and the fluorescence intensity in Escherichia coli 43R can even be observed in a single cell. These results concerning the co-expression of the genes coding for lumazine protein and for riboflavin synthesis raise the possibility to generate fluorescent bacteria which can be used in the field of bio-imaging. Full article
(This article belongs to the Special Issue Application of Smart Fluorescent Proteins for Biosensing)
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