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Genetically-Encoded Fluorescent Sensors in Plants and Fungi

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: closed (20 February 2022) | Viewed by 2809

Special Issue Editors


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Guest Editor
Institute of Biosciences and Bioresources, National Research Council of Italy (CNR-IBBR), Italy
Interests: Genetically encoded sensors,Plants and Fungi

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Guest Editor
Sainsbury Laboratory, University of Cambridge (SLCU), UK
Interests: Plants and Fungi

Special Issue Information

Genetically encoded sensors have revolutionized the way we measure molecules and metabolic states in vivo, non invasively and with high spatial and temporal resolutions. Recent avances in sensor design and the availability of fluorescent proteins with different spectral properties offer the possibility of monitoring several targets in real time in living cells and tissues. In plants, genetically encoded sensors are unravelling novel aspects of plant physiology and metabolism.

This Special Issue welcomes all papers related to the design and use of genetically encoded sensors in plants and fungi.

Dr. Roberto De Michele
Dr. Annalisa Rizza
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • genetically encoded sensors
  • biosensors
  • optogenetics
  • plant physiology
  • plants
  • fungi
  • metabolism
  • cell signalling

Published Papers (1 paper)

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Research

11 pages, 11808 KiB  
Article
Concept of Fluorescent Transport Activity Biosensor for the Characterization of the Arabidopsis NPF1.3 Activity of Nitrate
by Yen-Ning Chen and Cheng-Hsun Ho
Sensors 2022, 22(3), 1198; https://0-doi-org.brum.beds.ac.uk/10.3390/s22031198 - 04 Feb 2022
Cited by 4 | Viewed by 2360
Abstract
The NRT1/PTR FAMILY (NPF) in Arabidopsis (Arabidopsis thaliana) plays a major role as a nitrate transporter. The first nitrate transporter activity biosensor NiTrac1 converted the dual-affinity nitrate transceptor NPF6.3 into fluorescence activity sensors. To test whether this approach is transferable to [...] Read more.
The NRT1/PTR FAMILY (NPF) in Arabidopsis (Arabidopsis thaliana) plays a major role as a nitrate transporter. The first nitrate transporter activity biosensor NiTrac1 converted the dual-affinity nitrate transceptor NPF6.3 into fluorescence activity sensors. To test whether this approach is transferable to other members of this family, screening for genetically encoded fluorescence transport activity sensor was performed with the member of the NPF family in Arabidopsis. In this study, NPF1.3, an uncharacterized member of NPF in Arabidopsis, was converted into a transporter activity biosensor NiTrac-NPF1.3 that responds specifically to nitrate. The emission ratio change of NiTrac-NPF1.3 triggered by the addition of nitrate reveals the important function of NPF1.3 in nitrate transport in Arabidopsis. A functional analysis of Xenopus laevis oocytes confirmed that NPF1.3 plays a role as a nitrate transporter. This new technology is applicable in plant and medical research. Full article
(This article belongs to the Special Issue Genetically-Encoded Fluorescent Sensors in Plants and Fungi)
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