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Spectroscopy and Sensors for the Analysis of Microbes

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Optical Sensors".

Deadline for manuscript submissions: closed (31 October 2022) | Viewed by 2668

Special Issue Editor

Department of Physics, University of Auckland, Auckland 1010, New Zealand
Interests: fluorescence; Raman spectroscopy; infrared spectroscopy

Special Issue Information

Dear Colleagues,

Human awareness and the utilization of microbes continues to increase due to the influence of microbiomes on health and the ability to use microbes for production and processing. For example, our understanding of the gut microbiome and its role in health is rapidly evolving, as is our understanding of how the soil microbiome influences soil fertility. Microbes are used to ferment foods, to achieve bioremediation, and to produce pharmaceuticals and fuels, while pathogenic microbes may cause infectious diseases. The need for technologies to characterize and count microbes and to understand microbial processes is high and has led to a wide range of spectroscopic and sensing methodologies being developed.

This Special Issue will focus on spectroscopy and sensors applied to the analysis of microbes.   

Dr. Cushla McGoverin
Guest Editor

Manuscript Submission Information

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Keywords

  • archaea
  • bacteria
  • protists
  • fungi
  • algae
  • spectroscopy
  • biosensors
  • machine learning
  • chemometrics
  • sensing and imaging

Published Papers (1 paper)

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Research

9 pages, 1467 KiB  
Article
Simultaneous Raman and Infrared Spectroscopy of Stable Isotope Labelled Escherichia coli
by Cassio Lima, Howbeer Muhamadali and Royston Goodacre
Sensors 2022, 22(10), 3928; https://0-doi-org.brum.beds.ac.uk/10.3390/s22103928 - 22 May 2022
Cited by 12 | Viewed by 2262
Abstract
We report the use of a novel technology based on optical photothermal infrared (O-PTIR) spectroscopy for obtaining simultaneous infrared and Raman spectra from the same location of the sample allowing us to study bacterial metabolism by monitoring the incorporation of 13C- and [...] Read more.
We report the use of a novel technology based on optical photothermal infrared (O-PTIR) spectroscopy for obtaining simultaneous infrared and Raman spectra from the same location of the sample allowing us to study bacterial metabolism by monitoring the incorporation of 13C- and 15N-labeled compounds. Infrared data obtained from bulk populations and single cells via O-PTIR spectroscopy were compared to conventional Fourier transform infrared (FTIR) spectroscopy in order to evaluate the reproducibility of the results achieved by all three approaches. Raman spectra acquired were concomitant with infrared data from bulk populations as well as infrared spectra collected from single cells, and were subjected to principal component analysis in order to evaluate any specific separation resulting from the isotopic incorporation. Similar clustering patterns were observed in infrared data acquired from single cells via O-PTIR spectroscopy as well as from bulk populations via FTIR and O-PTIR spectroscopies, indicating full incorporation of heavy isotopes by the bacteria. Satisfactory discrimination between unlabeled (viz. 12C14N), 13C14N- and 13C15N-labeled bacteria was also obtained using Raman spectra from bulk populations. In this report, we also discuss the limitations of O-PTIR technology to acquire Raman data from single bacterial cells (with typical dimensions of 1 × 2 µm) as well as spectral artifacts induced by thermal damage when analyzing very small amounts of biomass (a bacterium tipically weighs ~ 1 pg). Full article
(This article belongs to the Special Issue Spectroscopy and Sensors for the Analysis of Microbes)
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