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Optical Immunosensors II
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Optical Immunosensors II

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Optical Sensors".

Deadline for manuscript submissions: closed (30 April 2022) | Viewed by 4566

Special Issue Editors

Dr. Elena Benito-Peña

E-Mail Website
Guest Editor
Department of Analytical Chemistry, Faculty of Chemistry, Complutensian University of Madrid, 28040 Madrid, Spain
Interests: optical (bio)sensing; bioinspired materials; biotechnology; analytical chemistry
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. David Gimenez-Romero

E-Mail Website
Guest Editor
Physical Chemistry Department, Faculty of Chemistry, University of Valencia, Avda. Dr. Moliner, 50, 46100 Valencia, Spain
Interests: (bio)sensing; reaction mechanism; kinetics; protein–protein interactions
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Volume will be dedicated to optical immunosensing. For decades, these sensors have been finding their way into an increasing number of industrial, environmental, pharmaceutical, medical, food quality control, and engineering applications. Optical immunosensors cost-effectively improve the reliability and efficiency of control systems, making them in great demand. Thus, this Special Issue aims to publish research with a broad scope covering all the aspects of optical sensing. According to the working principle of the transducer, optical immunosensors are based on absorption, reflectance, luminescence (fluorescence and phosphorescence), reflectance, chemi/bioluminescence, thermo/electrochemiluminescence, Raman spectrometry–surface-enhanced Raman spectroscopy (SERS)–refractometry–surface Plasmon resonance (SPR) and interferometry–photoacoustic, photoluminescence, and photoelectrochemistry. Examples of immunosensors include antibody-based imaging, antibody-based microarrays, and miniaturized optical immunosensors, as well as the incorporation to immunosensing platforms of nanomaterials such as quantum and carbon dots, graphene, metal–organic frameworks, and nucleic acid origami, among many others for plasmonic and photonic configurations.

This Special Issue welcomes both reviews and original research articles in the field of optical immunosensors. Topics include but are not restricted to optical fiber platforms, liquid crystals, optical waveguide light-mode spectroscopy (OWLS), white light reflectance spectroscopy (WLRS), and dual-polarization interferometry (DPI). Combinations with biological or bioinspired receptors such as aptamers, dendrimers, bacteriophages, affibodies, nanobiohybrid materials, and molecularly imprinted polymers are also of interest. There is no limit to the chemical and biological aspects by which an optical immunosensor can be manufactured. This issue emphasizes both the biological and transduction aspects of optical immunosensors. Please refer to Volume I here.

Dr. Elena Benito-Peña
Prof. Dr. David Gimenez-Romero
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Immunosensor
  • Immunoassay
  • Immunoanalysis
  • Bioimaging
  • Platforms
  • Biochip
  • Protein chips
  • Immunochips
  • Microarrays
  • Multiplexed analysis
  • Enzyme-linked immunosorbent assay (ELISA)
  • Surface plasmon resonance (SPR)
  • Surface-enhanced Raman spectroscopy (SERS)
  • Förster resonance energy transfer (FRET)
  • Optical waveguide light-mode spectroscopy (OWLS)
  • White light reflectance spectroscopy (WLRS)
  • Dual-polarization interferometry (DPI)
  • Evanescent wave
  • Label-free immunosensors
  • Protein immobilization
  • Antigen immobilization
  • Antibody immobilization
  • Microfluidic chips
  • Immuno-PCR
  • Bioreceptors
  • Antibody
  • Recombinant antibody
  • Nanobody
  • Mimotope
  • Phages
  • Phage-display
  • Aptamer
  • Affibodies
  • Molecularly imprinted polymers
  • Nanomaterials
  • Nanostructures
  • Nanoparticles
  • Quantum dots
  • Nanotubes
  • Nanowires

Published Papers (2 papers)

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Research

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9 pages, 1476 KiB  
Article
Improving the Quantification of Colorimetric Signals in Paper-Based Immunosensors with an Open-Source Reader
by Steven M. Russell, Alejandra Alba-Patiño, Andreu Vaquer, Antonio Clemente and Roberto de la Rica
Sensors 2022, 22(5), 1880; https://0-doi-org.brum.beds.ac.uk/10.3390/s22051880 - 27 Feb 2022
Cited by 2 | Viewed by 2141
Abstract
Measuring the colorimetric signals produced by the biospecific accumulation of colorimetric probes and recording the results is a key feature for next-generation paper-based rapid tests. Manual processing of these tests is time-consuming and prone to a loss of accuracy when interpreting faint and [...] Read more.
Measuring the colorimetric signals produced by the biospecific accumulation of colorimetric probes and recording the results is a key feature for next-generation paper-based rapid tests. Manual processing of these tests is time-consuming and prone to a loss of accuracy when interpreting faint and patchy signals. Proprietary, closed-source readers and software companies offering automated smartphone-based assay readings have both been criticized for interoperability issues. Here, we introduce a minimal reader prototype composed of open-source hardware and open-source software that has the benefits of automatic assay quantification while avoiding the interoperability issues associated with closed-source readers. An image-processing algorithm was developed to automate the selection of an optimal region of interest and measure the average pixel intensity. When used to quantify signals produced by lateral flow immunoassays for detecting antibodies against SARS-CoV-2, results obtained with the proposed algorithm were comparable to those obtained with a manual method but with the advantage of improving the precision and accuracy when quantifying small spots or faint and patchy signals. Full article
(This article belongs to the Special Issue Optical Immunosensors II)
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Review

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20 pages, 1068 KiB  
Review
Recent Developments in the Field of Optical Immunosensors Focusing on a Label-Free, White Light Reflectance Spectroscopy-Based Immunosensing Platform
by Chrysoula-Evangelia Karachaliou, Georgios Koukouvinos, Dimitrios Goustouridis, Ioannis Raptis, Sotirios Kakabakos, Evangelia Livaniou and Panagiota Petrou
Sensors 2022, 22(14), 5114; https://0-doi-org.brum.beds.ac.uk/10.3390/s22145114 - 07 Jul 2022
Cited by 8 | Viewed by 1869
Abstract
Optical immunosensors represent a research field of continuously increasing interest due to their unique features, which can mainly be attributed to the high-affinity and specific antibodies they use as biorecognition elements, combined with the advantageous characteristics of the optical transducing systems these sensors [...] Read more.
Optical immunosensors represent a research field of continuously increasing interest due to their unique features, which can mainly be attributed to the high-affinity and specific antibodies they use as biorecognition elements, combined with the advantageous characteristics of the optical transducing systems these sensors employ. The present work describes new developments in the field, focusing on recent bioanalytical applications (2021–2022) of labeled and label-free optical immunosensors. Special attention is paid to a specific immunosensing platform based on White Light Reflectance Spectroscopy, in which our labs have gained specific expertise; this platform is presented in detail so as to include developments, improvements, and bioanalytical applications since the mid-2000s. Perspectives on the field are been briefly discussed as well, highlighting the potential of optical immunosensors to eventually reach the state of a reliable, highly versatile, and widely applicable analytical tool suitable for use at the Point-of-Care. Full article
(This article belongs to the Special Issue Optical Immunosensors II)
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