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Separations
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Analysis of Complex Samples: Liquid Gas and Supercritical Fluid Chromatography...
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Analysis of Complex Samples: Liquid Gas and Supercritical Fluid Chromatography Methods

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Materials in Separation Science".

Deadline for manuscript submissions: closed (15 March 2022) | Viewed by 4581

Special Issue Editor

Dr. Mariosimone Zoccali

E-Mail Website
Guest Editor
Department of Mathematical and Computer Science, Physical Sciences and Earth Sciences, University of Messina, 98166 Messina, Italy
Interests: GC-MS; SFC-MS; GC×GC-MS; LC-GC; SFE-SFC-MS
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

It is a pleasure to announce the next Separations Special Issue, entitled “Analysis of Complex Samples: Liquid Gas and Supercritical Fluid Chromatography Methods”, which will compile the state-of-the-art methods developed in these areas.

The current trends in analytical chemistry are focused on the development of robust, sensitive, and simple methodologies that allow the simultaneous determination of a huge number of compounds; thus, this Special Issue is focused on separations of complex samples, broadly defined.

The aim of this Special Issue is to collect both original research papers and review articles able to highlight advancements in the use of chromatography in different fields and stimulate dialogue about major obstacles that are still present.

I thank all authors for submitting their interesting contributions, the referees for their on-time reviews, and the editorial team of Separations for their professional collaboration and encouragement.

Dr. Mariosimone Zoccali
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Gas chromatography
  • Liquid chromatography
  • Supercritical fluid chromatography
  • Volatiles
  • Semivolatiles
  • Complex samples

Published Papers (2 papers)

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Research

9 pages, 278 KiB  
Communication
Benefits of a Mixed-Mode Stationary Phase to Address the Challenging Purification of an Industrially Relevant Peptide: A Proof-of-Concept Study
by Giulio Lievore, Desiree Bozza, Martina Catani, Alberto Cavazzini, Tatiana Chenet, Luisa Pasti, Lucia Ferrazzano, Walter Cabri, Marco Macis, Antonio Ricci, Chiara De Luca and Simona Felletti
Separations 2022, 9(5), 125; https://0-doi-org.brum.beds.ac.uk/10.3390/separations9050125 - 17 May 2022
Cited by 1 | Viewed by 2197
Abstract
Peptides are a class of biomolecules with a great potential from the therapeutic point of view, because of their unique biological properties. Industrially, the production stategies adopted produce both the target peptide and a series of impurities that must be removed. Preparative chromatography [...] Read more.
Peptides are a class of biomolecules with a great potential from the therapeutic point of view, because of their unique biological properties. Industrially, the production stategies adopted produce both the target peptide and a series of impurities that must be removed. Preparative chromatography is the technique of choice for the large-scale purification of biomolecules, generally performed in reversed-phase mode, using hydrophobic adsorbents (e.g., C8 stationary phases). A promising and innovative alternative is represented by mixed-mode columns, which bear two different ligands on the particle surface, exploiting two different retention mechanisms to improve the separation. This work represents a proof-of-concept study focused on the comparison of a hydrophobic adsorbent and a mixed-mode one (bearing both hydrophobic groups and charged ones) for the purification of a crude peptide mixture. Thanks to more-favourable thermodynamics, it was found that, when collecting the whole peak excluding fractions of the peak tail, the mixed-mode column led to an increase in the recovery of roughly +15%, together with a slight improvement in purity at the same time, with respect to the traditional hydrophobic column. In addition, if the whole peak, including the tail, is collected, the performance of the two columns are similar in terms of purity and recovery, but the pepetide elutes as a narrower peak with the mixed mode. This leads to a collection pool showing a much-higher peptide concentration and to lower solvent volumes needed, which is a beneficial achievement when targeting more sustainable processes. These results are very advantageous from the industrial viewpoint, because they also involve a decrease in the peptide amount contained in the peak tail, which must be reprocessed again to satisfy purity requirements. Full article
(This article belongs to the Special Issue Analysis of Complex Samples: Liquid Gas and Supercritical Fluid Chromatography Methods)
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13 pages, 1848 KiB  
Article
Profiling the Volatile and Non-Volatile Compounds along with the Antioxidant Properties of Malted Barley
by Fouad El Mansouri, Hammadi El Farissi, Francesco Cacciola, Joaquim C. G. Esteves da Silva, Miguel Palma Lovillo, Yassine Oulad El Majdoub, Emanuela Trovato, Luigi Mondello, Mohamed Khaddor and Jamal Brigui
Separations 2022, 9(5), 119; https://0-doi-org.brum.beds.ac.uk/10.3390/separations9050119 - 12 May 2022
Cited by 1 | Viewed by 1909
Abstract
In this work, the stability of antioxidant compounds in malting barley seeds before and after the production of the final products is reported. In this reflection, the findings revealed that the process of fermentation had a significant impact on antioxidant activity. In vitro, [...] Read more.
In this work, the stability of antioxidant compounds in malting barley seeds before and after the production of the final products is reported. In this reflection, the findings revealed that the process of fermentation had a significant impact on antioxidant activity. In vitro, antioxidant capacities were evaluated using DPPH free radical scavenging assay. The results obtained from the spectrophotometric analysis showed that the lowest inhibition value was observed in the samples that were obtained by the classical fermentation process (ABC) and the samples of non-alcoholic beer obtained by the thermal process (NABT), with free radical inhibition capacity values of 8.50% and 5.50%, respectively. The samples of hopped wort (HW) and malted barley seeds extract (BSE) showed very high antioxidant activity with free radical inhibition capacity of 14% and 12.60%, respectively. The obtained extracts were analyzed by gas chromatography and high-performance liquid chromatography, both combined with mass spectrometry detection (GC–MS, HPLC–MS). GC–MS analysis of the SPME extraction showed the presence of 29 compounds with isopentyl alcohol in major concentration (18.19%) in the alcoholic beer; on the other hand, the HPLC–DAD–ESI/MS analysis of the ethyl acetate extract showed the presence of 13 phenolic compounds. Interestingly, the degradation of 3-Hydroxyphloretin 2′-O-glucoside in the final products of the non-alcoholic beers was found. Finally, the FTIR analysis was also employed in order to detect the type of efficient groups present in the extracts. Full article
(This article belongs to the Special Issue Analysis of Complex Samples: Liquid Gas and Supercritical Fluid Chromatography Methods)
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