Electrophoretic Methodologies for Protein Analysis

A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: closed (30 November 2020) | Viewed by 10994

Special Issue Editor


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Guest Editor
Institut des Biomolécules Max Mousseron (IBMM), Université de Montpellier– CNRS, UMR 5247, 34095 Montpellier, France
Interests: electrophoretic methodologies; miniaturized analytical techniques; pharmaceutical analysis; biomolecule analysis
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Special Issue Information

Dear Colleagues,

Electrophoretic methodologies are essential in the field of protein analysis whether for characterization, purification, quantification or protein–protein interaction purposes. In addition to mild operating conditions, the versatility of electrophoretic protocols provides unique separation abilities suitable for the analysis of both hydrophilic and hydrophobic proteins in various matrices. The potential of electrophoretic methods for miniaturization as well as their recent hyphenation to mass spectrometry has dramatically expanded their application areas both for top–down to bottom–up approaches.

The target of this Special Issue is to present the state of art in protein analysis by electrophoretic methodologies in conventional, capillary, and microchip format. Recent, original, and efficient methodologies are reported for all protein analysis domains.

Prof. Dr. Catherine Perrin
Guest Editor

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Keywords

  • Capillary electrophoresis
  • Electrophoretic methodologies
  • Electrophoretic µ-chip
  • Electrophoretic techniques and hyphenation
  • Free solution electrophoresis
  • Isoelectric focusing (IEF)
  • Polyacrylamide gel electrophoresis (PAGE)—sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
  • Protein analysis
  • Protein characterization
  • Protein–protein interactions
  • Protein purification

Published Papers (1 paper)

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Review

30 pages, 1376 KiB  
Review
Analysis of Monoclonal Antibodies by Capillary Electrophoresis: Sample Preparation, Separation, and Detection
by Meriem Dadouch, Yoann Ladner and Catherine Perrin
Separations 2021, 8(1), 4; https://0-doi-org.brum.beds.ac.uk/10.3390/separations8010004 - 04 Jan 2021
Cited by 25 | Viewed by 14234
Abstract
Therapeutic monoclonal antibodies (mAbs) are dominating the biopharmaceutical field due to the fact of their high specificity in the treatment of diverse diseases. Nevertheless, mAbs are very complex glycoproteins exhibiting several macro- and microheterogeneities that may affect their safety, quality, and efficacy. This [...] Read more.
Therapeutic monoclonal antibodies (mAbs) are dominating the biopharmaceutical field due to the fact of their high specificity in the treatment of diverse diseases. Nevertheless, mAbs are very complex glycoproteins exhibiting several macro- and microheterogeneities that may affect their safety, quality, and efficacy. This complexity is very challenging for mAbs development, formulation, and quality control. To tackle the quality issue, a combination of multiple analytical approaches is necessary. In this perspective, capillary electrophoresis has gained considerable interest over the last decade due to the fact of its complementary features to chromatographic approaches. This review provides an overview of the strategies of mAbs and derivatives analysis by capillary electrophoresis hyphenated to ultraviolet, fluorescence, and mass spectrometry detection. The main sample preparation approaches used for mAb analytical characterization (i.e., intact, middle-up/down, and bottom-up) are detailed. The different electrophoretic modes used as well as integrated analysis approaches (sample preparation and separation) are critically discussed. Full article
(This article belongs to the Special Issue Electrophoretic Methodologies for Protein Analysis)
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