Special Issue "Bacterial Pore-Forming Toxin"

A special issue of Toxins (ISSN 2072-6651). This special issue belongs to the section "Bacterial Toxins".

Deadline for manuscript submissions: 30 November 2021.

Special Issue Editor

Dr. Alexander Solonin
E-Mail Website
Guest Editor
Laboratory of molecular microbiology, G. K. Skryabin Institute of Biochemistry and physiology of microorganisms RAS, 5 Prospekt Nauki, Pushchino, Moscow Region 142290, Russia
Interests: bacterial pore-forming toxins; structure; function; evolution; regulation of toxin gene expression; monoclonal antibodies

Special Issue Information

Dear Colleagues,

The presence of direct evidence of the distribution of pathogenic microorganisms resistant to a wide range of antibiotics and the effective horizontal transfer of resistance genes to them has led to a situation in which antibiotics do not always help to cope with the infection. This requires the development of new approaches in the fight against bacterial infections. The study of the regulation of the expression of bacterial toxins is a basis for the implementation of directed control of the pathogenesis process. Finding ways to disrupt the adaptation of bacteria to various conditions and/or to hinder the expression of toxins, their secretion from the bacterial cell, and the implementation of separate stages of pore formation is certainly urgent. Therefore, a detailed knowledge of the principles of pore formation from genes to obtaining a mature pore makes it possible to determine the places of possible restriction or complete blocking of both the expression of genes of such toxins and their functional activity. Hence, it makes it possible to create an alternative anti-virulent therapy for many diseases, the course of which is determined by bacterial pore-forming toxins. The development of anti-virulence approaches will make it possible to obtain an alternative treatment for bacterial infections by acting directly on pathogenic factors, and not on a pathogenic microorganisms, including the design of monoclonal antibodies, toxin-specific antibodies, and small-molecule inhibitors against pore-forming proteins.

This current Issue is specifically focused on publishing research activities towards developing novel strategies for the diagnosis and suppression of pore-forming toxins. The Issue is expected to publish original research articles, reviews, and short communications in the broad area of pore-forming toxins research.

Dr. Alexander Solonin
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a double-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Toxins is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • anti-virulence therapy
  • bacterial pore-forming toxins
  • monoclonal antibodies
  • toxin expression regulation

Published Papers (1 paper)

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Research

Article
A Novel and Efficient High-Yield Method for Preparing Bacterial Ghosts
Toxins 2021, 13(6), 420; https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13060420 - 13 Jun 2021
Viewed by 931
Abstract
Bacterial ghosts (BGs) are empty cell envelopes possessing native extracellular structures without a cytoplasm and genetic materials. BGs are proposed to have significant prospects in biomedical research as vaccines or delivery carriers. The applications of BGs are often limited by inefficient bacterial lysis [...] Read more.
Bacterial ghosts (BGs) are empty cell envelopes possessing native extracellular structures without a cytoplasm and genetic materials. BGs are proposed to have significant prospects in biomedical research as vaccines or delivery carriers. The applications of BGs are often limited by inefficient bacterial lysis and a low yield. To solve these problems, we compared the lysis efficiency of the wild-type protein E (EW) from phage ΦX174 and the screened mutant protein E (EM) in the Escherichia coli BL21(DE3) strain. The results show that the lysis efficiency mediated by protein EM was improved. The implementation of the pLysS plasmid allowed nearly 100% lysis efficiency, with a high initial cell density as high as OD600 = 2.0, which was higher compared to the commonly used BG preparation method. The results of Western blot analysis and immunofluorescence indicate that the expression level of protein EM was significantly higher than that of the non-pLysS plasmid. High-quality BGs were observed by SEM and TEM. To verify the applicability of this method in other bacteria, the T7 RNA polymerase expression system was successfully constructed in Salmonella enterica (S. Enterica, SE). A pET vector containing EM and pLysS were introduced to obtain high-quality SE ghosts which could provide efficient protection for humans and animals. This paper describes a novel and commonly used method to produce high-quality BGs on a large scale for the first time. Full article
(This article belongs to the Special Issue Bacterial Pore-Forming Toxin)
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