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Viruses
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7th Pan-American Dengue Research Network Meeting: Lima 2020
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7th Pan-American Dengue Research Network Meeting: Lima 2020

A special issue of Viruses (ISSN 1999-4915). This special issue belongs to the section "General Virology".

Deadline for manuscript submissions: closed (30 June 2021) | Viewed by 38092

Special Issue Editors

Dr. Carlos A. Sariol

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Guest Editor
Unit of Comparative Medicine, Caribbean Primate Research Center, Department of Microbiology and Medical Zoology, Department of Internal Medicine, University of Puerto Rico-Medical Sciences Campus, San Juan, Puerto Rico
Interests: basic research on flavivirus interactions at molecular level and pathogenesis; vaccine development and characterization of novel viruses in non human primate model; also interested and actively involved in clinical trials for several diseases including flavivirus vaccines
Special Issues, Collections and Topics in MDPI journals
Dr. Mariana Leguia

E-Mail Website
Guest Editor
Director, Genomics Laboratory, Pontificia Universidad Católica del Perú, Lima, Peru
Interests: study of tropical and neglected infectious diseases using state-of-the-art genomic tools, including ribosome profiling. Particular focus on pathogen discovery and characterization, host-pathogen interactions and changes in gene expression, and evaluation of vaccine efficacy using systems biology approaches
Special Issues, Collections and Topics in MDPI journals
Dr. Daniela Weiskopf

E-Mail Website
Guest Editor
La Jolla Institute for Immunology, Division of Vaccine Discovery
Interests: T cell response against flaviviruses in general and dengue virus; the characterization of cellular mediated immunity in the context of vaccination and acute disease

Special Issue Information

Dear Colleagues,

PanDengue Net is a non-profit initiative that gathers scientists, researchers, and medical professionals working in the field of arbovirology across the Americas (http://www.pandenguenet.org). The PanDengue Net meeting, which is an event scheduled every two years, brings these researchers together in the context of a 4-day scientific gathering. The meeting allows for the presentation and discussion of recent advances in the field. The main focus is on Dengue, Zika, Chikungunya, and other arboviruses of medical relevance that severely impact public health in the Americas and around the world.

As in previous meetings (2008 Recife, Brazil; 2010 Cancun, Mexico; 2012 Cartagena, Colombia; 2014 Belém, Brazil; 2016 Panama City, Panama; and 2018 Galveston, Texas, USA), the Lima meeting will include pre-eminent scientists from a wide range of fields, including vaccine development, vector research and control, molecular epidemiology, diagnostics, antivirals, viral structure and pathogenesis, and viral immunology. The meeting is essential to the fostering of collaborations among groups and to the discussion of future research strategies needed to strengthen the field. Importantly, the meeting also provides a unique platform for students and junior investigators from across Latin America and the Caribbean, who are usually under-represented in science, to come together under one roof with leading figures in the field.

Dr. Carlos A. Sariol
Dr. Mariana Leguia
Dr. Daniela Weiskopf
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Viruses is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Dengue
  • Zika
  • Chikungunya
  • Yellow fever
  • Mayaro
  • vaccine
  • pathogenesis
  • immunology
  • virology
  • community intervention
  • vector control.

Related Special Issues

Published Papers (5 papers)

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Research

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17 pages, 972 KiB  
Article
Different Profiles of Cytokines, Chemokines and Coagulation Mediators Associated with Severity in Brazilian Patients Infected with Dengue Virus
by Victor Edgar Fiestas Solórzano, Nieli Rodrigues da Costa Faria, Caroline Fernandes dos Santos, Gladys Corrêa, Márcio da Costa Cipitelli, Marcos Dornelas Ribeiro, Luiz José de Souza, Paulo Vieira Damasco, Rivaldo Venâncio da Cunha, Flavia Barreto dos Santos, Luzia Maria de Oliveira Pinto and Elzinandes Leal de Azeredo
Viruses 2021, 13(9), 1789; https://0-doi-org.brum.beds.ac.uk/10.3390/v13091789 - 08 Sep 2021
Cited by 7 | Viewed by 2712
Abstract
The incidence of dengue in Latin America has increased dramatically during the last decade. Understanding the pathogenic mechanisms in dengue is crucial for the identification of biomarkers for the triage of patients. We aimed to characterize the profile of cytokines (IFN-γ, TNF-α, IL-1β, [...] Read more.
The incidence of dengue in Latin America has increased dramatically during the last decade. Understanding the pathogenic mechanisms in dengue is crucial for the identification of biomarkers for the triage of patients. We aimed to characterize the profile of cytokines (IFN-γ, TNF-α, IL-1β, IL-6, IL-18 and IL-10), chemokines (CXCL8/IL-8, CCL2/MCP-1 and CXCL10/IP-10) and coagulation mediators (Fibrinogen, D-dimer, Tissue factor-TF, Tissue factor pathway inhibitor-TFPI and Thrombomodulin) during the dengue-4 epidemic in Brazil. Laboratory-confirmed dengue cases had higher levels of TNF-α (p < 0.001), IL-6 (p = 0.005), IL-10 (p < 0.001), IL-18 (p = 0.001), CXCL8/IL-8 (p < 0.001), CCL2/MCP-1 (p < 0.001), CXCL10/IP-10 (p = 0.001), fibrinogen (p = 0.037), D-dimer (p = 0.01) and TFPI (p = 0.042) and lower levels of TF (p = 0.042) compared to healthy controls. A principal component analysis (PCA) distinguished between two profiles of mediators of inflammation and coagulation: protective (TNF-α, IL-1β and CXCL8/IL-8) and pathological (IL-6, TF and TFPI). Lastly, multivariate logistic regression analysis identified high aspartate aminotransferase-to-platelet ratio index (APRI) as independent risk factors associated with severity (adjusted OR: 1.33; 95% CI 1.03–1.71; p = 0.027), the area under the receiver operating characteristics curve (AUC) was 0.775 (95% CI 0.681–0.869) and an optimal cutoff value was 1.4 (sensitivity: 76%; specificity: 79%), so it could be a useful marker for the triage of patients attending primary care centers. Full article
(This article belongs to the Special Issue 7th Pan-American Dengue Research Network Meeting: Lima 2020)
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17 pages, 4667 KiB  
Article
Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment
by Jorge G. G. Ferreira, Sandra G. Gava, Eneida S. Oliveira, Izabella C. A. Batista, Gabriel da R. Fernandes, Marina M. Mourão and Carlos E. Calzavara-Silva
Viruses 2020, 12(11), 1284; https://0-doi-org.brum.beds.ac.uk/10.3390/v12111284 - 10 Nov 2020
Cited by 7 | Viewed by 2650
Abstract
Dengue is an acute viral disease caused by Dengue virus (DENV) and is considered to be the most common arbovirus worldwide. The clinical characteristics of dengue may vary from asymptomatic to severe complications and severe organ impairment, particularly affecting the liver. Dengue treatment [...] Read more.
Dengue is an acute viral disease caused by Dengue virus (DENV) and is considered to be the most common arbovirus worldwide. The clinical characteristics of dengue may vary from asymptomatic to severe complications and severe organ impairment, particularly affecting the liver. Dengue treatment is palliative with acetaminophen (APAP), usually known as Paracetamol, being the most used drug aiming to relieve the mild symptoms of dengue. APAP is a safe and effective drug but, like dengue, can trigger the development of liver disorders. Given this scenario, it is necessary to investigate the effects of combining these two factors on hepatocyte homeostasis. Therefore, this study aimed to evaluate the molecular changes in hepatocytes resulting from the association between DENV infection and treatment with sub-toxic APAP concentrations. Using an in vitro experimental model of DENV-2 infected hepatocytes (AML-12 cells) treated with APAP, we evaluated the influence of the virus and drug association on the transcriptome of these hepatocytes by RNA sequencing (RNAseq). The virus–drug association was able to induce changes in the gene expression profile of AML-12 cells and here we highlight and explore these changes and its putative influence on biological processes for cellular homeostasis. Full article
(This article belongs to the Special Issue 7th Pan-American Dengue Research Network Meeting: Lima 2020)
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10 pages, 2263 KiB  
Article
Efficient Method for Molecular Characterization of the 5′ and 3′ Ends of the Dengue Virus Genome
by Alicia Rosales-Munar, Diego Alejandro Alvarez-Diaz, Katherine Laiton-Donato, Dioselina Peláez-Carvajal and Jose A. Usme-Ciro
Viruses 2020, 12(5), 496; https://0-doi-org.brum.beds.ac.uk/10.3390/v12050496 - 29 Apr 2020
Cited by 7 | Viewed by 3596
Abstract
Dengue is a mosquito-borne disease that is of major importance in public health. Although it has been extensively studied at the molecular level, sequencing of the 5′ and 3′ ends of the untranslated regions (UTR) commonly requires specific approaches for completion and corroboration. [...] Read more.
Dengue is a mosquito-borne disease that is of major importance in public health. Although it has been extensively studied at the molecular level, sequencing of the 5′ and 3′ ends of the untranslated regions (UTR) commonly requires specific approaches for completion and corroboration. The present study aimed to characterize the 5′ and 3′ ends of dengue virus types 1 to 4. The 5′ and 3′ ends of twenty-nine dengue virus isolates from acute infections were amplified through a modified protocol of the rapid amplification cDNA ends approach. For the 5′ end cDNA synthesis, specific anti-sense primers for each serotype were used, followed by polyadenylation of the cDNA using a terminal transferase and subsequent PCR amplification with oligo(dT) and internal specific reverse primer. At the 3′ end of the positive-sense viral RNA, an adenine tail was directly synthetized using an Escherichia coli poly(A) polymerase, allowing subsequent hybridization of the oligo(dT) during cDNA synthesis. The incorporation of the poly(A) tail at the 5′ and 3′ ends of the dengue virus cDNA and RNA, respectively, allowed for successful primer hybridization, PCR amplification and direct sequencing. This approach can be used for completing dengue virus genomes obtained through direct and next-generation sequencing methods. Full article
(This article belongs to the Special Issue 7th Pan-American Dengue Research Network Meeting: Lima 2020)
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Review

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19 pages, 1409 KiB  
Review
Current Understanding of the Role of T Cells in Chikungunya, Dengue and Zika Infections
by Maheshi Mapalagamage, Daniela Weiskopf, Alessandro Sette and Aruna Dharshan De Silva
Viruses 2022, 14(2), 242; https://0-doi-org.brum.beds.ac.uk/10.3390/v14020242 - 25 Jan 2022
Cited by 13 | Viewed by 5563
Abstract
Arboviral infections such as Chikungunya (CHIKV), Dengue (DENV) and Zika (ZIKV) are a major disease burden in tropical and sub-tropical countries, and there are no effective vaccinations or therapeutic drugs available at this time. Understanding the role of the T cell response is [...] Read more.
Arboviral infections such as Chikungunya (CHIKV), Dengue (DENV) and Zika (ZIKV) are a major disease burden in tropical and sub-tropical countries, and there are no effective vaccinations or therapeutic drugs available at this time. Understanding the role of the T cell response is very important when designing effective vaccines. Currently, comprehensive identification of T cell epitopes during a DENV infection shows that CD8 and CD4 T cells and their specific phenotypes play protective and pathogenic roles. The protective role of CD8 T cells in DENV is carried out through the killing of infected cells and the production of proinflammatory cytokines, as CD4 T cells enhance B cell and CD8 T cell activities. A limited number of studies attempted to identify the involvement of T cells in CHIKV and ZIKV infection. The identification of human immunodominant ZIKV viral epitopes responsive to specific T cells is scarce, and none have been identified for CHIKV. In CHIKV infection, CD8 T cells are activated during the acute phase in the lymph nodes/blood, and CD4 T cells are activated during the chronic phase in the joints/muscles. Studies on the role of T cells in ZIKV-neuropathogenesis are limited and need to be explored. Many studies have shown the modulating actions of T cells due to cross-reactivity between DENV-ZIKV co-infections and have repeated heterologous/homologous DENV infection, which is an important factor to consider when developing an effective vaccine. Full article
(This article belongs to the Special Issue 7th Pan-American Dengue Research Network Meeting: Lima 2020)
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35 pages, 521 KiB  
Review
Dengue: A Minireview
by Harapan Harapan, Alice Michie, R. Tedjo Sasmono and Allison Imrie
Viruses 2020, 12(8), 829; https://0-doi-org.brum.beds.ac.uk/10.3390/v12080829 - 30 Jul 2020
Cited by 145 | Viewed by 21815
Abstract
Dengue, caused by infection of any of four dengue virus serotypes (DENV-1 to DENV-4), is a mosquito-borne disease of major public health concern associated with significant morbidity, mortality, and economic cost, particularly in developing countries. Dengue incidence has increased 30-fold in the last [...] Read more.
Dengue, caused by infection of any of four dengue virus serotypes (DENV-1 to DENV-4), is a mosquito-borne disease of major public health concern associated with significant morbidity, mortality, and economic cost, particularly in developing countries. Dengue incidence has increased 30-fold in the last 50 years and over 50% of the world’s population, in more than 100 countries, live in areas at risk of DENV infection. We reviews DENV biology, epidemiology, transmission dynamics including circulating serotypes and genotypes, the immune response, the pathogenesis of the disease as well as updated diagnostic methods, treatments, vector control and vaccine developments. Full article
(This article belongs to the Special Issue 7th Pan-American Dengue Research Network Meeting: Lima 2020)
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