Special Issue "State-of-the-Art Plant Virus Research in China"

A special issue of Viruses (ISSN 1999-4915). This special issue belongs to the section "Viruses of Plants, Fungi and Protozoa".

Deadline for manuscript submissions: 30 April 2022.

Special Issue Editor

Prof. Dr. Feng Li
E-Mail Website
Guest Editor
College of Horticultural Sciences and Forestry, Huazhong Agricultural University, Wuhan 430070, China
Interests: plant-virus interaction; RNA silencing
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Plant viruses impose serious threats to agriculture in China, and there is a big consortium of scientists working on basic plant virology and its applications in engineering viral-resistant crops. In the past few years, numerous important advancements have been achieved by Chinese scientists in elucidating mechanisms of viral pathogenesis, plant resistance to viral infection, and trilateral plant–virus–vector interactions. Many useful viral vectors for forward genetics and gene editing have also been developed which have made significant contributions to dissecting genetic mechanisms in plants. New advancements have also been seen in the discovery of new viral and viroid pathogens in crops, as well as the ecology of known viruses among important crops in China. All these new achievements have provided essential information and knowledge for agricultural researchers to engineering viral-resistant crops for deployment in specific areas. In this special issue, we welcome submissions for comprehensive reviews or new research advancements in—but not limited to—all the above mentioned areas.

Prof. Dr. Feng Li
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Viruses is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • RNA silencing
  • autophage
  • viral suppressor of RNA silencing
  • pathogenesis
  • resistance
  • R gene
  • crop
  • insect
  • VIGS
  • gene editing

Published Papers (1 paper)

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Research

Article
Genome Editing of Rice eIF4G Loci Confers Partial Resistance to Rice Black-Streaked Dwarf Virus
Viruses 2021, 13(10), 2100; https://0-doi-org.brum.beds.ac.uk/10.3390/v13102100 - 18 Oct 2021
Viewed by 477
Abstract
Rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (RBSDV), is a serious constraint in Chinese rice production. Breeding disease-resistant varieties through multigene aggregation is considered an effective way to control diseases, but few disease-resistant resources have been characterized thus far. To [...] Read more.
Rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (RBSDV), is a serious constraint in Chinese rice production. Breeding disease-resistant varieties through multigene aggregation is considered an effective way to control diseases, but few disease-resistant resources have been characterized thus far. To develop novel resources for resistance to RBSDV through CRISPR/Cas9-mediated genome editing, a guide RNA sequence targeting exon 1 of eIF4G was designed and cloned into a binary vector, pHUE401. This recombinant vector was used to generate mutations in the rice cultivar Nipponbare via Agrobacterium-mediated transformation. This approach produced heritable homozygous mutations in the transgene-free T1 generation. Sequence analysis of the eIF4G target region from T1 transgenic plants identified 3 bp deletion mutants, and analysis of the predicted amino acid sequence identified one amino acid deletion in mutants that possess near full-length eIF4G. Furthermore, our data suggest that eIF4G may plays an important role in rice normal development, as there were no eIF4G knock-out homozygous mutants in T1 generation plants. When homozygous mutant lines were inoculated with RBSDV, they exhibited enhanced tolerance to virus infection, without visibly affecting plant growth and development. However, the eif4g mutant plants showed the same sensitivity to rice stripe virus (RSV) infection as wild-type plants. Notably, the wild-type and mutant N-termini of eIF4G interacted directly with RBSDV P8 in yeast and in planta. Additionally, compared to wild-type plants, the eIF4G transcript level was reduced twofold in the mutant plants. These results indicate that site-specific mutation of rice eIF4G successfully conferred partial resistance specific to RBSDV associated with less transcription of eIF4G in mutants. Therefore, this study demonstrates that the novel eIF4G alleles generated by CRISPR/Cas9 represent valuable disease-resistant resources that can be used to develop RBSDV-resistant varieties. Full article
(This article belongs to the Special Issue State-of-the-Art Plant Virus Research in China)
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