Basic Substances, a Sustainable Tool to Complement and Eventually Replace Synthetic Pesticides in the Management of Pre and Postharvest Diseases: Reviewed Instructions for Users
by
, ,
Gianfranco Romanazzi
1,*
,
Yann Orçonneau
2, 
Marwa Moumni
1
,
Yann Davillerd
2 and
Patrice André Marchand
2 1
Department of Agricultural, Food and Environmental Sciences, Marche Polytechnic University, Via Brecce Bianche, 60131 Ancona, Italy
2
Institut Technique de l’Agriculture et de l’Alimentation Biologiques (ITAB), 149 rue de Bercy, 75012 Paris, France
*
Author to whom correspondence should be addressed.
Molecules 2022, 27(11), 3484; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27113484
Submission received: 15 April 2022
/
Revised: 17 May 2022
/
Accepted: 24 May 2022
/
Published: 28 May 2022
(This article belongs to the Special Issue Chitosan and Natural Compounds for the Control of Preharvest and Postharvest Diseases of Horticultural Commodities)
Abstract
:Synthetic pesticides are widely used to protect crops from pathogens and pests, especially for fruits and vegetables, and this may lead to the presence of residues on fresh produce. Improving the sustainability of agriculture and, at the same time, reducing the adverse effects of synthetic pesticides on human health requires effective alternatives that improve the productivity while maintaining the food quality and safety. Moreover, retailers increasingly request fresh produce with the amounts of pesticides largely below the official maximum residue levels. Basic substances are relatively novel compounds that can be used in plant protection without neurotoxic or immune-toxic effects and are still poorly known by phytosanitary consultants (plant doctors), researchers, growers, consumers, and decision makers. The focus of this review is to provide updated information about 24 basic substances currently approved in the EU and to summarize in a single document their properties and instructions for users. Most of these substances have a fungicidal activity (calcium hydroxide, chitosan, chitosan hydrochloride, Equisetum arvense L., hydrogen peroxide, lecithins, cow milk, mustard seed powder, Salix spp., sunflower oil, sodium chloride, sodium hydrogen carbonate, Urtica spp., vinegar, and whey). Considering the increasing requests from consumers of fruits and vegetables for high quality with no or a reduced amount of pesticide residues, basic substances can complement and, at times, replace the application of synthetic pesticides with benefits for users and for consumers. Large-scale trials are important to design the best dosage and strategies for the application of basic substances against pathogens and pests in different growing environments and contexts.
1. Introduction
The world population continues to grow and will reach 9.7 billion by 2050 [1]. For this, increasing food production is the primary objective of all countries. According to the latest estimates of the Food and Agriculture Organization of the United Nations [2], up to 40% of food crops worldwide are lost every year due to pests and plant diseases. Crop losses caused by plant disease alone cost the global economy $220 billion annually [3]. Crop protection is essential to reduce yield losses, improve food quality, and increase grower profitability. The application of plant protection products (PPPs) is the main way to protect crops against pathogens, pests, and weeds [4]. However, human, animal, and environmental risks associated with the use of chemical PPPs are a growing concern. All these concerns have encouraged the onset of research to develop alternative approaches to control plant diseases [5]. Reducing the use of pesticides being a major challenge in developed countries, European Union Member States are required to implement National Action Plans that set quantitative objectives, timetables, and indicators related to reducing the impact of pesticide use (Directive 2009/128/CE) [6,7]. The use of basic substances is approved in the European Union under Article 23 of EC Regulation No 1107/2009 and which are listed in Part C of the Annex of the Regulation (EC) No 540/2011 [8]. In the EU, Integrated Pest Management (IPM) has been mandatory since January 2014, and among the rules of the IPM is the reduction of the application of synthetic pesticides whenever possible [9]. For sustainable and qualitative food production, respectful of the need to produce in sufficient quantities, biocontrol has grown tremendously through the last few years [10]. The PPP EU Regulation (EC) 1107/2009 was established to ensure a level of protection of humans, animals, and the environment and, at the same time, to unify for the entire EU the rules on the placing on the market of plant protection products [11,12]. Basic substances are sources of interest for research as alternative to synthetic pesticides, since they are used in human medicine or as a food ingredient, so they have no residue concerns and then no maximum residue limit (MRL) and, usually, no preharvest interval [13,14]. The lack of MRL contributes to a better prevention of contamination in plant protection, a better control of the residues and a reduction of analytical problems, of decommissioning, and of market withdrawal [14]. Another benefit of basic substances, and perhaps the most important, is their very low ecologic impact. Basic substances are products that are used as ‘foodstuffs’, as defined in Article 2 of Regulation (EC) 178/2002 [15] cosmetic, and does not have an inherent capacity to cause endocrine-disrupting, neurotoxic or immunotoxic effects, but they are also plant protection means and not placed on the market as a plant protection product. Article 28 of Regulation (EC) No. 1107/2009 set the absence of marketing authorizations and usages allowance for basic substances. Regulation (EC) No. 1107/2009 introduced the new category of ‘basic substances’, which are defined by recital 18 as ‘certain substances which are not predominantly used as plant protection products may be of value for plant protection, but the economic interest of applying for approval may be limited. Therefore, specific provisions should ensure that such substances, as far as their risks are acceptable, may also be approved for plant protection use’. The properties of basic substances are described in Article 23 of the EU Regulation (EC) No 1107/2009 [11]. In 2021, the Euphresco project ‘BasicS’ contributed to demonstrate the effectiveness toward pests and pathogens of basic substances, with potential benefits for the farmers, the consumer, and the environment [16,17]. The basic substances have a positive impact on crop health when applied preventively. Certain basic substances, such as chitosan, stimulate the defense system of crops against several classes of pathogens, including fungi, viruses, bacteria, and phytoplasma [18]. According to the EU pesticides database, 24 basic substances were approved for use, 7 were withdrawn, 18 applications were not approved and 8 are still pending [19,20]. This review includes currently approved basic substances that have a protective potential and are a valuable addition to the range of measures and protection methods intended for use. Detailed information about basic substances and updates on new available compounds can be found at the page https://ec.europa.eu/food/plant/pesticides/eu-pesticides-database/active-substances (accessed on 23 May 2022). The standard-folder for approval of a basic substance, called ‘Basic Substance Application Template (BSAT)’, is based on the structure of the European Union evaluation report of an active substance that can be used for plant protection purposes. BSAT refers to all areas of risk assessment in the regulation of phytopharmaceutical product uses and shall be considered as a structured model to build a file collating all available information and enabling to demonstrate that the evaluated substance meets the eligibility criteria of a basic substance (SANCO 10,363 rev.10, 2021). Therefore, nowadays, a full deposit under International Uniform ChemicaL Information Database (IUCLID) software is mandatory since March 2021. Basic substances are submitted individually (Annex I inclusion dossier) at the first stage; then, later, an automatic inclusion was adopted for food/foodstuff basic substance from plant or animal origin [21,22]. Recently, an automatic consideration procedure (without any Annex I inclusion dossier) by Expert Group for Technical advice on Organic Production (EGTOP)/Directorate-General for the Agriculture and Rural Development (DGAgri) of positive ongoing basic substance approval (from Directorate-General Health and Food Safety—DGSanté to DGAgri) to generate an automatic EGTOP/DGAgri outcome for inclusion (or not). This provision bypasses the traditional route of substances in organic production in plant protection through dossiers submitted to Member States, but so far, no basic substance has been rejected by the Regulatory Committee of Organic Production (RCOP), and with the current procedure, are no longer studied than substances of mineral origin (or non-foods).
This review aimed to highlight the properties of approved basic substances, summarize, and provide this information for phytosanitary consultants, scientists, growers, stakeholders, companies, and consumers.
2. Results
Out of the 86 basic substance application submitted to the European Commission until now, less than one-third have been approved (24) (Table 1 and Table 2), 19 have been refused, 6 have been withdrawn during their assessment (Table 3), 8 are currently being processed by the EC (Table 4 and Figure 1), and 2 already successfully submitted via IUCLID software (Ginger extract and Capsicum frutescens).
Currently, 24 basic substances are approved, of which 21 are also approved in organic production; for example, talc was validated in 2021 following EGTOP PPP VII and is being currently voted on at RCOP [23] and clayed charcoal was submitted. Recently, voted chitosan does not seem to be acceptable directly in organic production as the basic substance from its microorganism’s origin, although in the context of food quality. Basic substances are approved by EU Regulations, so the application month, where reported in Table 1, is related to the Northern Hemisphere.
The scientific literature dealing with basic substances is relatively limited but increasing in recent years (Figure 2), and there is poor information about the effectiveness in field trials of basic substances toward pests and pathogens.
In the last decade, MRLs for pesticides with agricultural trade are becoming important. In the EU, there are increasing requirements from retailers to their suppliers to provide fruits and vegetables with an amount of pesticide residue below the MRLs (Table 5).
The substances tested during Casdar programs ‘4P’, ‘Carie’, ‘Sweet’, ‘HE, Ecophyto ‘Usage’ and some from projects have already been described (Marchand, 2016) (Table 6). New projects are ongoing to develop extensions of use, describe better efficacy through better positioning during the season or to investigate compatibility/incompatibility with other biocontrol agents (i.e., reduce copper and macro-organisms). This is the ongoing work for Coperreplace, ABAPIC (ITAB), Vitinnova (UNIVPM), and Euphresco BasicS (Euphresco Network).
3. Discussion
The use of pesticides, if not appropriate, may lead to problems like contamination of the water, potential damage to sensitive species (e.g., bees), contamination of final food products and water, with up to 90% of applied pesticides not reaching the target species, and, also, because of the development of resistant pathogens and pests [39]. A high number of PPPs were not reauthorized (or companies did not provide the dossier for the reregistration of products out of patent, due to high costs and uncertain benefits) and leaves a gap for several uses. It is important that authorities provide a good number of options to growers to protect their crops, since farmers cannot stand without PPPs for certain crops and uses, and there is an increasing need, because a lot of substance prohibition dates are fixed without substitution mean. Just as an example, this occurred with the fungicide mancozeb in January 2022 and a risk to occur in 2025 with copper, that is fundamental for plant protection in organic agriculture and a good support to prevent the appearing of resistant isolates in IPM. In France, the use of neonicotinoids, known as dangerous insecticides, is extended when there is no other way to preserve crops and productivity. With Farm to Fork Strategy of the European Green Deal, the European Commission is committed to reduce the use of the most dangerous synthetic pesticides of 50% and achieve at least 25% of the EU agricultural land under organic farming by 2030, although the decrease of synthetic pesticides is already ongoing. These trends, together with the implementation of sustainable development goals—SDGs by the United Nations—are demanding for new alternatives, such as basic substances, to tackle some of these issues. To achieve these goals, more research is needed to advance the design of better farming systems and the development of alternatives to synthetic pesticides and to copper formulations.
Three decades ago, the concept of MRLs was poorly known, while, in recent years, MRLs for pesticides arguably have become the first action growers should consider in their pest management decisions [40]. Trying to interpret consumer demands, retailers are increasingly required to reduce pesticide residues even more than the allowed thresholds (MRLs), which are defined considering a wide security factor (e.g., ×100) using the presence of pesticide residues as a factor of competition among companies. Requests from the retailers and consumer to reduce synthetic pesticide residues from fresh produce even more than the allowed threshold, such that the rules defined by the public administration have become more limiting for farmers in terms of the active ingredients allowed and MRLs [40,41]. The reduction of the presence of fungicide residues well beyond MRL may allow the pathogen to develop after harvest, resulting food loss and waste along the value chain. These developments have driven the search for alternative management strategies that are effective and not reliant just on conventional fungicide applications [5,42,43]. European regulation followed and carried this development with the introduction of new classes of phytosanitary products, in particular basic substances, but also new laws and simplification accompanied by the reduction of registration processes of low-risk substances, theoretically. Basic substances are approved for use in the EU and are products that are already sold for certain purposes, e.g., as a foodstuff or a cosmetic. Basic substances may be of major importance in biocontrol and several advantages can explain it. Basic substance regulatory application is simplified [44] and particularly reduced compared to other substances, therefore representing a lower cost to applicant (around 35-40 kEuro for approval of a basic substance and overall around 45 kEuro including approval for organic agriculture), thanks to the fact that these substances are already on the market for another purpose than plant protection, and safety is not an issue to be demonstrated. These substances are good alternatives available today and wide targets. Basic substances can be used in the crop protection as fungicide, bactericide, insecticide, etc., and most of them are allowed in organic production [18,45,46,47]. The basic substances are in order from 2014, when was the first approved application of Equisetum arvense L., chitosan hydrochloride, and sucrose until 2022, when a second chitosan formulation was approved. In some conditions basic substances were already at farm level, with a level of pest management not different than the standard. Just as example, chitosan hydrochloride was also applied in commercial conditions, in the field, and postharvest treatments, and several studies proved that it could have an effectiveness comparable to some commercial PPPs [42,48]. Basic substances, probably less efficient and practical to use than other active substances authorized as PPPs, are known and used by producers since decades as substitution means and have already demonstrated their effectiveness. Basic substances were the perfect tool to provide to producers as known, easy-to-use, less dangerous, and environmentally more respectful. Today, there is a consensus among a wide range of stakeholders that synthetic pesticide used need to be gradually reduced to a level that is effectively required to ensure crop production and that risks of pesticide application should be reduced as far as possible. Basic substances are good alternatives available today in our hands. The use of these substances needs to be integrated in vocational education, training, and technical advice to farmers. Further research around the world on the efficacy of basic substances may prove in the future that these substances can replace pesticides without reducing yields or increasing production costs. To develop the uses and the field trials we listed here the main usages of basic substances. However, rates included in the approval schedule may not produce a significant containment of diseases and pests in specific pathosystems. Just as example, the advised application rate of chitosan hydrochloride is between 100 and 800 g/ha, equal to a concentration ranging among 0.05 and 0.2% with 200–400 L/ha, while trials in commercial vineyards found a good effectiveness delivering the chitosan hydrochloride, with a concentration of at least 0.5% and with a volume of at least 500 L/ha [34,49]. For this reasons, large-scale trials are very important to demonstrate the effectiveness toward pathogens and pests in different environments and growing contexts, and a flexibility could be required in suggested dosages to avoid that applying basic substances at suggested rated can lead to a lack of or poor effectiveness and then the disaffection of users toward these innovative compounds, and this is in contrast with the requirements of finding solutions alternatives to the application of synthetic pesticides keeping the standard quality and quantity of the production, which is one of the drivers of the Farm-to=Fork Strategy of European Green Deal. Moreover, the diluent allowed for basic substance, up to now concretely restricted to water, may be another substance. In this case, vinegar has just been authorized for chitosan. Finally, increasing the demand from growers and competition among companies can lead to the reduction of costs of the treatments that, nowadays, are often higher than standard treatments.
4. Materials and Methods
4.1. Collection of Data
A systematic literature search from 2009 to 2021 was performed using the database of Scopus with the keywords ‘basic substance’ and ‘basic substances’. In the EU, several retailers request an amount of pesticide residue on fruit and vegetables below the legal limit (MRL), and data on some protocols were collected through companies and plant doctors.
4.2. Legislation
Basic substance criteria are defined by article 23 of Regulation (EC) No. 1107/2009, cited in introduction. By way of derogation from Article 4 of this regulation, a basic substance is approved when all relevant evaluations conducted in accordance with other Community legislation, governing other uses of this substance, showing that it has neither an immediate or delayed harmful effect on human or animal health nor any unacceptable influence on the environment. Active substances that could be defined as ‘foodstuff’ are intrinsically considered as basic substances, following Article 2 of Regulation (EC) No. 178/2002. Basic substances shall be approved in accordance with paragraphs 2–6 of regulation (EC) No. 1107/2009 and by way of derogation from Article 5, the approval shall be for an unlimited period. By way of derogation from Article 7 of Regulation (EC) No. 1107/2009, an application for approval of a basic substance can be made by a Member State or any interested party. At the end of the evaluation process, basic substances shall be listed separately in the Regulation referred to in Article 13(4). The Commission may review the approval of an active substance at any time. It may take into account the request of a Member State to review the approval. Article 28 of Regulation (EC) No. 1107/2009 set the absence of marketing authorizations and usages allowance for basic substances. However, no formal authorization is required as long as the product contains exclusively basic substances (see corresponding Review Report) [49,50].
4.3. Approval Process
The approval process of a basic substance starts with a request for approval (Figure 3). The applicant estimates if the substance concerned fulfil all criteria of basic substances category and then complete the BSAT, in English, to obtain a Basic Substance Application. Several guidance documents, such as the official SANCO guide or the teaching guide from the ITAB, have been published to help applicants to build basic substance application correctly [50]. For the transmission of the basic substance application, once completed, the file should be sent to the DGSanté, representing the European Commission (EC). The Basic Substance Application can firstly be sent to national competent authorities for a preassessment and possibly a support. For example, in France, the Basic Substance Application can be sent to the Ministry of Agriculture (DGAl in France), who can ask for the National Authority’ opinion and then transfer the file to the EC. Upon receipt of the Basic Substance Application, EC implements the approval procedure detailed in Article 23 of Regulation (EC) No. 1107/2009. Admissibility may be pronounced at any time, directly or after questions from DGSanté. It constitutes the real start of the application (black line in Figure 3). The first stage is based on the Basic Substance Application evaluation by Member States and EFSA as scientific assistance leading to a request for corrections and questions. The request is sent to the applicant, and his answers shall be sent back within one month to the EFSA. For decision and approval, at the end of the basic substance application evaluation, EFSA will deliver its opinion, append a comment, and send the basic substance application to the DG Health within 3 months for the final vote of Member States in the PAFF committee (Figure 3). Approval, if accorded, is effective at the date of the publication of an implementing Regulation modifying Regulation (EU) No. 540/2011 [8].
The period of examination of the basic substance application is established in paragraph 1 of article 37 of Regulation (EC) No. 1107/2009. It is said: ‘The Member State examining the application shall decide within 12 months of receiving it whether the requirements for authorization are met. Where the Member State needs additional information, it shall set a period for the applicant to supply it. In that case, the 12-month period shall be extended by the additional period granted by the Member State. That additional period shall be a maximum of 6 months and shall cease at the moment when the additional information is received by the Member State. Where at the end of that period the applicant has not submitted the missing elements, the Member State shall inform the applicant that the application is inadmissible.’ [10]. The maximum delay is therefore set at 18 months. However, although clearly defined, these steps are not so straightforward in many cases [51].
4.4. Extension of Uses Process
The request for an extension is somehow similar, except the need of support from corresponding agricultural sectors at the deposit step. Some extensions were voted after submission, some others were granted with admissibility and voted rapidly after; some later were following the full approval pathway, including admissibility, evaluation, outcome, full vote at PAFF Committee (appearance in Part A (lecture, discussion), C (proposal) and B (effective vote)). This latter process sometimes takes the same amount of time compared to a new approval, which is considered very excessive by the applicants, having an approved substance at the beginning of their request and only asking for one line sometimes in the Good Agricultural Practices (GAP) table.
4.5. Regulation Analysis
The EU Pesticides Database [52] was used to detect basic substances and their status (approved, nonapproved, pending, and modifications of Review Reports). Corresponding linked Implementing Regulations [20] attached to each active substance were found using the same method and cross-verified with Implementing Regulation (EU) 540/2011. The EU law database for Eur-Lex was also used to track each Implementing Regulation publication. Furthermore, EFSA documents were also compiled to extract decisions supportive analyses.
5. Conclusions
Searching for alternative products for crop protection is an important strategy for promoting more sustainable food systems. The use of basic substances is in line with the restriction on the application of chemical PPPs and the principles of the European Green Deal and SDGs, mostly renewables and with no MRL. There is relatively poor information about the effectiveness of basic substances as compared to synthetic pesticides and biological PPPs. A higher testing and validation of the use of basic substances as a phytosanitary measure can lead to further reduction of application of synthetic pesticides. In addition, searching for the most effective dosage of the basic substance is critical and an important question for phytosanitary consultants (the plant doctors that are opinion leaders in application of innovations in pest management), growers, stakeholder, and companies to avoid that their application at the recommended dose can lead to a lack of or poor effectiveness of these substances. For this reason, a flexibility might be required in the suggested dosage of basic substances approved to ensure good maintenance of the quality and quantity of production, which is one of the keys of the Farm to Fork Strategy of the European Green Deal. Moreover, a defined timeline for approval is basilar to have the chance to increase the number of basic substances available for growers, the scientific community, and the whole agricultural sector, with final benefits for the consumers.
6. Patents
All Implementing Regulations may be considered as patents but with free exploitation, since no Marketing Authorizations are needed for basic substances.
Supplementary Materials
The following supporting information can be downloaded at: https://0-www-mdpi-com.brum.beds.ac.uk/article/10.3390/molecules27113484/s1, Figure S1: Time needed for Basic Substance Application admissibility evaluation over time (bars) and tendency line (dotted line); Table S1: Total time of basic substance application process within admissibility to Implementing Regulation publication in months.
Author Contributions
Conceptualization, G.R. and P.A.M.; methodology, Y.O. and M.M.; resources, G.R., Y.O., Y.D., M.M. and P.A.M.; writing—original draft preparation, G.R., Y.O., Y.D., M.M. and P.A.M.; writing—review and editing, G.R., Y.D., M.M. and P.A.M.; supervision, G.R. and P.A.M.; and funding acquisition, G.R. and P.A.M. All authors have read and agreed to the published version of the manuscript.
Funding
For G.R. and M.M., this work was conducted within the framework of the PSR ZeroSprechi, PSR Vitinnova, PSR CleanSeed, and of PRIMA StopMedWaste projects, which are funded by PRIMA, a program supported by the European Union. For Authors Y.O., Y.D., and P.A.M., the French Ministry of Agriculture (CASDAR ‘4P’, ‘Contrat de branche Carie’, ‘Sweet’, and ‘HE’; Ecophyto ‘Usage’, ’Biocontrol’ ’INADOM’, and ’PARMA’), French Ministry of Ecology (Project ‘PNPP‘ CT0007807, ‘SubDOMEx‘, and ‘Jussie‘). All authors worked within the “Euphresco BasicS (Objective 2020-C-353) project. Thanks are expressed to Antonello Lepore, Gianni Ceredi and other technicians for providing data about pesticide residues requested by retailers.
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
Data is contained within the article or supplementary material.
Conflicts of Interest
The authors declare no conflict of interest.
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Figure 1.
Total of the basic substance applications (BSA) and extensions presented by the results (%).
Figure 1.
Total of the basic substance applications (BSA) and extensions presented by the results (%).
Figure 2.
Number of documents available on Scopus through searches with keywords ‘basic substances’ in ‘Article title, Abstract, and Keywords’ (histograms) or in ‘All fields’ (linear) published over the last 10 years (Source: Scopus, https://www.scopus.com, accessed on 11 May 2022).
Figure 2.
Number of documents available on Scopus through searches with keywords ‘basic substances’ in ‘Article title, Abstract, and Keywords’ (histograms) or in ‘All fields’ (linear) published over the last 10 years (Source: Scopus, https://www.scopus.com, accessed on 11 May 2022).
Figure 3.
Approval process and timeline of a Basic Substance Application (BSA).
Table 1.
Application of the basic substances approved.
| Basic Substance | Approval Regulation and Applicant | Crops and/or Situation | Function in Plant Protection | Pests or Group of Pests Target | Application | Application Rates | Notes | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Method | Growth Stage & Season | No. Min/Max | IBA 1 (Days) | Min–Max | Water L/ha Min–Max | Total Rate | PHI 1 | ||||||
| Equisetum arvense L. | Reg. (EU) No 462/ 2014 ITAB | Fruit trees Apple fruit (Malus pumila, Malus domestica) Peach-tree (Prunus persica) | Fungicide | Foliar fungi like scab disease (Venturia inaequalis), Powdery mildews (Podosphaera leucotricha) Peach leaf curl (Taphrina deformans) | Foliar application spraying | From green leaf tip (BBCH 53) to flowers fading (BBCH 67) Spring | 2–6 | 7 | 200 g/hL | 500–1000 | 1000–2000 g/ha | Na 1 | Plant homogenate extracted with hot water and filtered to be used 24 h after preparation |
| Grapevine (Vitis vinifera) | Downy mildew (Plasmopara viticola), Powdery mildew (Erysiphe necator) | From 1st shoots (BBCH 10) to cluster tightening (BBCH 57) Spring to summer | 100–300 | 200–600 g/ha | Na | ||||||||
| Cucumber (Cucumis sativus) roots | Powdery mildew (Podosphaera fusca) Root fungi like common root rot, seedling blight (Pythium spp.) | Root feeding application and foliar application spraying | From (9th leaf unfolded on main stem—BBCH 19) to 9 or more primary side shoots visible (BBCH 49) | 2 | 3–4 | 300 | 600 g/ha | 15 | |||||
| Tomato (Lycopersicum esculentum) | Early blight (Alternaria solani), Septoria blight (Septoria lycopsersici) | Foliar application spraying | First inflorescence visible (BBCH 51) to BBCH 59 summer | 14 | |||||||||
| Strawberry (Fragaria × Ananassa) Raspberry (Rubus idaeus) | Gray mold (Botrytis cinerea), Powdery mildew (Podosphaera aphanis), red core (Phytophthora fragariae), other fungi like Colletotrichum acutatum | Foliar application spraying 2 | Growth restart till end of fructification. Early spring till end of summer Stage BBCH 1 to BBCH 89 | 4–8 | 5–14 | 225 g/hL | 300 | 675 g/ha | Na | ||||
| Potato (Solanum tuberosum) | Late blight (Phytophthora infestans), early blight (Alternaria solani), powdery mildew (Erysiphe cichoracearum) | Stage BBCH 1 until BBCH 9 | |||||||||||
| Ornamental trees use of which Prunus spp. Roses Rosa spp. | Ornamental fungal diseases, rose black spot (Marsonia spp.), Rose rust (Phragmidium mucronatum), leaf curl diseases, monilioses, oidium and mildew | Included in mulch | Not relevant | 1 | Na | Na | Na | 9000 g/ha | Dry plant aerial parts usage never applied on whole hectare | ||||
| Chitosan hydrochloride | Reg. (EU) 2021/1446 ChiPro | Fruits berries and small fruit | Elicitor, having a fungicide and bactericide effect via the stimulation of natural defence mechanisms | Plant elicitor, plant resistance against pathogenic fungi and bacteria | Low–Medium volume spraying | From 1 leaf development (main shoot) to 7 development of fruit | 4–8 | 14 | 50–200 g/hL | 200–400 | 100–800 g/ha | 0 | |
| Vegetables | 50–100 g/hL | 100–400 g/ha | |||||||||||
| Cereals | |||||||||||||
| Spices | |||||||||||||
| Crops for animal feed | |||||||||||||
| Cereals Seed treatment | Low volume spraying | Before sowing | 1 | Na | Na | Na | |||||||
| Potatoes Seed treatment | Low volume spraying/dipping | Na | Na | ||||||||||
| Sugar beet Seed treatment | 50–200 g/hL | Na | Na | ||||||||||
| Ornamental bulbous plants | Bulb treatment– Dipping/drenching | Germination (BBCH 00–01) | 50–100 g/hL | 200–800 | 100–800 g/ha | ||||||||
| Low–Medium volume spraying | Leaf development– senescence (BBCH 10–92) | 1–8 | 5–7 | 50–200 g/hL | 200–400 | ||||||||
| Low–Medium volume spraying | Leaf development –senescence (BBCH 10–92) | ||||||||||||
| Beet crops | |||||||||||||
| Sucrose | Reg. (EU) No 916/2014 ITAB IRBI | Apple trees/ orchards (Malus pumila, Malus domestica) | Elicitor, having an insecticidal and fungicidal effect via the stimulation of natural defence mechanisms | Fruits borer like Codling moth (Cydia pomonella) 3 | Foliar application spraying early in the morning before 9 AM (Solar time) | From spring BBCH stage 6 to summer BBCH stage 89 | 7–10 | 15 | 10 g/hL | 600–1000 | 60–100 g/ha | Na | Cold water solution prepared just before application |
| Sweet Maize (Sweet corn) (Zea mays L. convar. saccharata Koern) | Corn borer (Ostrinia nubilalis Hbn.) 3 | From the BBCH stage 12 to 89 | 3–4 | 200 | 20 g/ha | ||||||||
| Maize (corn grain) (Zea mays subsp. mays (L.)) and corn seed | Corn borer (Ostrinia nubilalis Hbn.) 3 | From the BBCH stage 12 to 51 | 3–4 | ||||||||||
| Grapevine (Vitis vinifera) | Vine leafhopper (Scaphoideus titanus) 3 | From the BBCH stage 17 to 57 | 3 | 7 | 150 | 15 g/ha | |||||||
| Grapevine (Vitis vinifera) | Downy mildew (Plasmopara viticola) 3 | From 1st shoots to cluster tightening spring (BBCH 10–57) | up to 2 | 100–200 | 10–20 g/ha | ||||||||
| Calcium hydroxide | Reg. (EU) 2015/762 IFOAM | Pome fruit | Fungicide | Neonectria galligena | Sprinkler application | Leaf drops end of October till end of December | 2–7 | 5–14 | 104–208 L/ha 4 1460 L/ha 5 | 5000–10,000 L/ha | 25–50 kg/ha 350 kg/ha 3 | Na | |
| Pome fruit and stone fruit | Neonectria galligena and other diseases | Spray application | With products at 24% 63–104 L/ha 4 728 L/ha 5 with products at 33.12% 45–76 L/ha 4 532 L/h5 | 500–1000 L/ha | 15–25 kg/ha 4 175 kg/ha 5 | ||||||||
| Brush application directly on pruning wounds and old cancers on stems 6 | Winter to March | 1–2 | 21 | With products at 24% 450 L/ha 3 900 L/ha 4 with products at 33.12% 450 L/ha 4 900 L/ha 5 | No extra water 6 | 149.04 kg 4 299.08 kg 5 | |||||||
| Vinegar | Reg. (EU) No 540/2011 Reg. (EU) 2015/1108 Reg. (EU) 2019/149 ITAB | Wheat seeds (Triticum vulgare), common wheat (Triticum aestivum), durum wheat (Triticum durum), spelt (Triticum spelta) | Fungicide, bactericide and herbicide | Common bunt (Tilletia caries, Tilletia foetida) | Seed treatment just before seeding | Autumn | 1 | Na | 25–50 7 per 100 kg of seed | Not applicable | 24–100 7,8 | Na | |
| Barley seeds (Hordeum vulgare) | Barley leaf stripe (Pyrenophora graminea) | ||||||||||||
| Market vegetables Gardening like carrot (Daucus carota), tomato (Solanum lycopersicum), bell pepper (Capsicum spp.) | Alternaria spp. | Autumn to spring | Seeds are tem-porary soaked in the dilution then removed | Seeds are temporary soaked in the preparation then removed | |||||||||
| Market vegetables gardening like tomato (Solanum Lycopersicum), bell pepper (Capsicum spp.), cabbage (Brassica oleracea) | Clavibacter michiganensis, Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato, Xanthomonas campestris pv. vesicatoria, Botrytis aclada | 1 | Na | ||||||||||
| White and red chestnut (Aesculus L.), Sycamore spp. (option), Acer spp. | Bacteria: Pseudomonas syringae pv. aesculi | Tools application before sawing or cutting 9 | Na | 1 per day to each time before use | 1 | 400 g/hL | Na | Na | Waiting period 30 s after washing | ||||
| Hawthorns (Rosaceae): Crataegus spp., Amelanchir, Aronia, Chaenomeles, Cotoneaster, Cydonia, Malus, Photinia, Potentilla, Prunus, Pyracantha, Pyrus, Rosa, Sorbus and Spiraea | Fire blight (Erwinia amylovora) | Na | Na | Na | Na | ||||||||
| Many ornamental plants including Acer, Cotoneaster, Euonymus, Forsythia, Magnolia, Philadelphus, Populus, Prunus, Pyrus, Rosa, Rubus, Syringa and Vaccinium | Bacterial blight /canker (Pseudomonas syringae pv. syringae) | Na | Na | Na | Na | ||||||||
| Plane sp., Platanus, Prunus sp., Chestnut sp., Aesculus L., Sophora spp., Linden sp., Tilia | Rot fungi, especially phellins: Phellinus, Tinder polypore and ruffled (Fomes fomentarius) | Na | Na | Na | Na | ||||||||
| Elm (elm other than Lutèce) (Ulmus spp.) | Vascular fungi: Ophiostoma spp. | Na | Na | Na | Na | Na | |||||||
| Maple sp., Acer sp. | Wilt disease | Na | Na | Na | Na | Na | |||||||
| Ailanthe sp., Ailanthus altissima | Verticillium spp. | Na | Na | Na | Na | Na | Na | Na | |||||
| Maple sp., Acer sp.; Sycamore, Acer spp.; Chestnut sp., Aesculus L.; Beech sp., Fagus spp. | Sooty-Bark disease (Cryptostroma corticale) | Na | Na | Na | Na | Na | Na | Na | |||||
| ITAB/ITEIPMAI | Medicinal aromatic and perfume crops | Weeds | Spray 10 | Pre crop emergence | 1 | Na | 10 kg/hL | 100 L vinegar (no dilution) | 10 kg/ha | >120 | Phytotoxic to plant, may kill the young plants 11 | ||
| Charbonneaux-Brabant | paths, borders, sidewalks and terraces | Weeds | Direct spray (spot application) | Vegetation Period of the weeds | 1–2 | 7–21 | 6 kg/hL | 100 L (diluted vinegar) | 6–12 kg/ha | Na | Temp > 20 °C phytotoxic to plant, may kill the young plants 12 | ||
| Salix spp. cortex | Reg. (EU) 2015/1107 ITAB | Fruit trees, Peach tree (Prunus persica) | Fungicide | Foliar fungi like Taphrina deformans | Foliar application spraying | From 1st shoots (BBCH 10) to cluster tightening (BBCH 57) spring | 2–6 | 7 | 222.2 g/hL | 500–1000 L/ha | 1111.1–2222.2 g/ha | Na | Plant homogenate extracted with hot water (infusion), filtered and diluted by 3, to be used up to a maximum of 24 h after preparation. The product cannot be applied in case of hot temperature. It is used in case of rainy period |
| Apple fruit (Malus pumila, Malus domestica) | Foliar fungi like scab disease (Venturia inaequalis), powdery mildew (Podosphaera leucotricha) | From green leaf tip (BBCH 53) to flowers fading (BBCH 67) spring | |||||||||||
| Grapevine (Vitis vinifera) | Downy mildew (Plasmopara viticola), Powdery mildew (Erysiphe necator) | From 1st shoots (BBCH 10) to cluster tightening (BBCH 57) spring to summer | 100–300 | 222.2–666.6 g/ha | |||||||||
| Lecithins | Reg. (EU) No 540/2011 Reg. (EU) 2015/1116 ITAB DAE | Fruit trees Apple fruit (Malus pumila) Peach tree (Prunus persica) | Fungicide | Powdery mildew (Podosphaera leucotricha) Peach leaf curl (Taphrina deformans) | Spray application | BBCH 03 to BBCH 79 | 3–12 | 5 | 75 g/hL | 500–1000 | 375–750 g/ha | 5 | |
| Gooseberry Ribes uva-crispa | Powdery mildew (Microsphaera grossulariae) | BBCH 10 to BBCH 85 | 2–4 | 200 g/hL | 1000– 2000 g/ha | ||||||||
| Market vegetables gardening like cucumber (Cucumis sativus) | Powdery mildew (Podosphaera fusca) | BBCH 10 to BBCH 89 | 2–6 | 150 g/hL | 1000– 1500 | 1500– 2250 g/ha | |||||||
| Lettuce (Lactuca sativa) | Erysiphe cichoracearum | 2 | 7 | ||||||||||
| Mash(Valerianella locusta) | Erysiphe polyphaga | 1 | Na | ||||||||||
| Tomato (Lycopersicum esculentum) | Tomato late blight (Phytophthora infestans) | 2 to 6 | 7 | ||||||||||
| Endive (Cichorium endivia L.) | Alternaria cichorii | ||||||||||||
| Ornamentals, especially roses | Powdery mildew and other fungal diseases | 3–12 | 5 | 75 g/hL | 100–300 | 75–225 g/ha | |||||||
| Grapevine (Vitis vinifera) | Downy mildew (Plasmopara viticola), Powdery mildew (Erysiphe necator) | BBCH 11 to BBCH 85 | 30 | ||||||||||
| Strawberry (Fragaria × Ananassa) Raspberry (Rubus idaeus) | Powdery mildew and other fungal diseases, i.e., Podosphaera aphanis, Red core (Phytophthora fragariae) | Growth restart till end of fructification Early spring till end of Summer Stage BBCH 10 to BBCH 89 (2nd crop, other strawberries have reached them specific color) | 200 g/hL | 300–500 | 600–1000 g/ha | Na | |||||||
| Potato (Solanum tuberosum) | Late blight (Phytophthora infestans) | Stage BBCH 10 until BBCH 90 | 3–12 | 100–400 | 200–800 g/ha | ||||||||
| Carrot (Daucus carota subsp. sativus) | Powdery mildew (Leveillula taurica) | BBCH 19 to BBCH 90 | 4 | 14 | 1000 | 2000 g/ha | |||||||
| Fructose | Reg. (EU) 2015/1392 ITAB IRBI | Apple fruit (Malus pumila, Malus domestica) | Elicitor, having an insecticidal and fungicidal effect via the stimulation of natural defence mechanisms | Fruits borer like Codling Moth (Cydia pomonella) 13 | Foliar application spraying early in the morning before 9 AM (solar time) | From spring BBCH stage 6 to summer BBCH stage 65 | 5–7 | 21 | 10 g/hL | 600–1000 | 60–100 g/ha | Na | Cold water solution prepared just before application |
| Maize (Corn grain) (Zea mays subsp. mays L.) Sweet Maize (Sweet corn) (Zea mays L. convar. saccharata Koern) | Symphylans (Scutigerella immaculata) 13 | Treatment in seedling line before 9 AM (solar time) | - | 1 | Na | 40 | 40 g/ha | ||||||
| Zea mays subsp. mays L. | Foliar application Spraying early in the morning before 9 AM (solar time) | 1 application at 2–3 leaves (BBCH 12–13) + 1 application at 4 leaves (BBCH 14) | 2 | 1–2 | 82 | 8.2 g/ha | |||||||
| Grapevine (Vitis vinifera) | Vine leafhopper (Scaphoideus titanus) 4 | Foliar application spraying early in the morning before 9 AM (solar time) | From the BBCH stage 17 to 57 | 3 | 3 | 150 | 15 g/ha | ||||||
| Grapevine (Vitis vinifera) | Downy mildew (Plasmopara viticola) 4 | From 1st shoots to cluster tightening Spring (BBCH 10–57) | up to 12 | >12 | 100–200 | 10–20 g/ha | |||||||
| Sodium hydrogen carbonate | Reg. (EU) 2015/2069 Reg. (EU) 2015/2069 Danish Environmental Protection Agency | Vegetables Soft fruit Ornamentals | Fungicide and herbicide | Mildews (Sphaerotheca spp., Oidium spp.) | Broad cast using field spray or greenhouse spray | BBCH 12 to 89 | 1–8 | 10 | 333–1000 g/hL | 300–600 | 2000–5000 g/ha or 0.33–1.0% Max 1% Dose adjusted depending on water volume | 1 | Different crops have different sensitivity. Check concentrations for phytotoxic effects before widely used |
| Grapevine (Vitis vinifera) | Powdery mildew (Erysiphe necator) | Broadcast using air blast orchard sprayer | BBCH 12 to 89 | 1–8 | 420–2000 g/hL | 200–600 | 2500–5000 g/ha or 0.42–2.0% | Volumes and doses will vary according to crop canopy size. Conc. higher than 1–2% can be phytotoxic | |||||
| Apple | Apple scab (Venturia inaequalis) | Broadcast using air blast orchard sprayer | BBCH 10 to 85 | 1–8 | 500–1000 g/hL | 500–1000 | 2500–5000 g/ha or 0.5–1.0% | ||||||
| Fruit of different types (oranges, cherries, apples, papaya) | Storage diseases like Blue mold (Penicillium italicum) Green mold (Penicillium digitatum) | Dipping or surface treatment | Harvested fruit | 1–2 | 1000–4000 g in 100 L water | 1–4% | Dose rates between 1–4% has been tested | ||||||
| Potted plants | Liverwort/ Bryophyte (thallose, Lunularia cruciata) Green thallus of liverwort plus, fruiting bodies | Direct application of powder | Post emergence late summer or winter | 1 | Na | Na | Na | 122 kg/ha | Na | The product is used for post emergence application. Phytotoxicity of this use was not tested, check on small number of plants before it is widely used | |||
| Whey | Reg. (EU) 2016/560 ITAB | Cucumber (Cucumis sativus), zucchini squash (Cucurbita pepo) | Fungicide and virucide | Podosphaera fusca, Podosphaera xanthii, Golovinomyces cichoracearum, Erysiphe orontii, Sphaerotheca fuliginea, Leveillula cucurbitacearum | Foliar spray 12 | From three weeks after sowing (9th leaf unfolded on main stem) to 9 or more primary side shoots visible (BBCH 19–49) 14 | 3–5 | 7 | 0.6–3 L (0.036 –0.24 kg/hL) | 1000–1500 | 6–30 L (0.36– 2.4 kg/ha) | Na | Whey should be used rapidly after collection, not stored in metal vessel |
| Grapevine (Vitis vinifera) | Powdery mildew(Erysiphe necator) | From 1st shoots to cluster tightening Spring 15 | 7–10 | 6–30 L (0.36–2.4 kg/hL) | 100–30,0 15 | 6–30 L (0.36– 2.4 kg/ha) | |||||||
| Vegetable Gardening, Tomato (Lycopersicum esculentum) | Tomato (Sinaloa) yellow leaf curl virus Begomovirus | First inflorescence visible Summer (BBCH 10–51) 15 | 3–4 | 0.6–3 L (0.036 –0.24 kg/hL) | 1000– 1500 | ||||||||
| Glove fingertips and mechanical cutting tools All crops | Viruses (Mechanically transferable) e.g., Tobacco mosaic virus (TMV), Tomato mosaic virus (ToMV), Pepper mild mottle virus (PMMV), Cucumber green mottle mosaic virus (CGMMV), Tomato brown rugose fruit virus (ToBRFV) | Dipping | On tools and glove fingertips | Before/after every plant contact 16 | Na | Na | Na | Na | Dipping for 5 s for gloves and 5 min for mechanical cutting tools. For reasons of efficacy use whey protein powder with at least 80% protein content. Replace the whey solution regularly (e.g., after each crop row) to prevent cross contamination of the plant | ||||
| Diammonium phosphate | Reg. (EU) 2016/548 ITAB | Orchards including cherry tree (Prunus spp.) | Attractant | Mediterranean fruit fly (Ceratitis capitata), Cherry fly (Rhagoletis cerasi) | Placed in physical traps | Na | Mass trapping: 1 trap per tree up to 100 traps/ha | 42–56 17 | max 4 kg/hL | Mass trapping: max 100 | Mass trapping: max 4 kg/ha | Na | |
| Olive trees (Olea europaea) | Olive fly (Bactrocera oleae) | ||||||||||||
| Citrus spp. | Mediterranean fruit fly (Ceratitis capitata) | ||||||||||||
| Other crops where C. capitata cause damage | |||||||||||||
| Sunflower oil | Reg. (EU) 2016/1978 ITAB | Tomato (Lycopersicum esculentum) | Fungicide | Tomato powdery mildew (Pseudoidium neolycopersici) | Foliar application spraying | BBCH 32–37 then BBCH 61–71 | 2 to 4 | 8 | 0.092 kg/hL (0.1 L) –0.46 kg/hL (0.5 L) | 500 to 1000 | 0.46 kg/hL (0.5 L)– 4.6 kg/hL (5 L) | 2 | Precautions must be taken to avoid overwatering and spilling of the dispersion. Treatment should be avoided during flowering time |
| Urtica spp. | Reg. (EU) 2017/419 ITAB | Fruit trees Apple tree (Malus domestica), Plum tree (Prunus domestica), Peach tree (Prunus persica), Red currant (Ribes rubrum), Walnut tree (Juglans sp.), Cherry tree (Prunus sp.) | Insecticide, fungicide, acaricide | Peach-potato Aphid (Myzus persicae, Macrosiphum rosae), wolly Apple aphid (Eriosoma lanigerum), Currant aphid (Cryptomyzus ribis), Walnut aphid (Callaphis juglandis), Black cherry aphid (Myzus cerasi) | Foliar spraying or Shoot spraying Directly on aphids | Spring summer until BBCH 87 (fruit ripe for picking) | 1–5 | 7–15 | 1500 g/hL (dry matter) 18 | 300–900 L/ha | 4500–13,500 g/ha 17 | 7 | Preventive treatment is inefficient 24 h of maceration at 20 °C is enough |
| Bean, for example French bean (Phaseolus vulgaris) | Black bean aphid (Aphis fabae) | Spring Summer until BBCH 89 (fully ripe) | 300–500 L/ha 18 | 4500–7500 g/ha 18 | |||||||||
| Potato (Solanum tuberosum) | Peach-potato aphid (Myzus persicae) | Na | Spring Summer until BBCH 49 (end of tuber formation) | Na | Na | Na | 4500–10,000 g/ha 17 | Na | |||||
| Leaf Vegetables: Lettuce (Lactuca sativa), Cabbage (Brassica olaeracea) | Aphids, for example: cabbage aphid (Brevicoryne brassicae), Nazonoviaribis nigri) | Foliar spraying or shoot spraying directly on aphids | Spring Summer until BBCH 19 (9 or more true leaves unfolded) | 1–5 | 7–15 | 1500 g/hL (dry matter) 18 | 4500– 7500 g/ha 18 | 7 | Preventive treatment is inefficient 24 h of maceration at 20 °C is enough | ||||
| Elder tree (Sambucus racemosa) | Elder aphid (Aphis sambuci) | Spring Summer | 400–800 | 6000– 12,000 g/ha 18 | |||||||||
| Rose (Rosa sp.) | Rose aphid (Macrosyphum rosae) | 300–600 | 4500–9000 g/ha 18 | ||||||||||
| Spiraea sp. | Aphis spiraephaga | ||||||||||||
| Brassicaceae (cabbage—Brassica oleracea, rapeseed— Brassica napus, radish— Raphanus sativus) | Fleabeetle (Phyllotreta nemorum) | Foliar spraying | Spring Summer Until BBCH 19 (9 or more true leaves unfolded | 1–6 | 300–500 | 4500–10,000 g/ha 18 | |||||||
| Diamondback moth (Plutella xylostella) | Spring Summer until BBCH 49 (Typical leaf mass reached) | ||||||||||||
| Apple tree (Malus domestica), Peer tree (Pyrus communis) | Codling moth (Cydia pomonella) | 2 treatments in April, 1 treatment in May | 3 | 15 | 300–900 | 4500–13,500 g/ha 18 | |||||||
| Bean, for example French bean (Phaseolus vulgaris) | Two-spotted spider mite (Tetranychus urticae) | Spring Summer Until BBCH 89 (fully ripe) | 1–6 (commonly 3) | 7–21 | 300 –500 | 4500–7500 g/ha 18 | 7 | 24 h of maceration at 20 °C is enough | |||||
| Grapevine (Vitis vinifera) | Two-spotted spider mite (Tetranychus urticae), red spider mite (Tetranychus telarius) | Spring Summer Until BBCH 89 stage | 1–6 (three before flowering, three after flowering) | 300–600 | 4500–9000 g/ha 18 | ||||||||
| Brassicaceae (Mustard family, Brassica sp., Sinapis sp., radish— Raphanus sativus) | Alternaria sp. | Foliar spraying | Spring Summer until BBCH 49 (typical leaf mass reached) | 1–6 | 7–15 | 1500 g/hL (Based on dry matter) 18 | 300–500 | 4500–7500 g/ha 18 | 7 | ||||
| Cucurbitaceae (Cucumber— Cucumis sativus) | Powdery mildew (Erysiphe polygoni), Alternaria alternata f. sp. cucurbitae | Until BBCH 89 (typical fully ripe colour) | |||||||||||
| Fruit trees (Apple trees— Malus domestica, Plum trees— Prunus domestica, Peach trees– Prunus persica, Sweet cherry tree— Prunus avium) | Leaf spot (Alternaria alternata), brown rot, blossom blight (Monilinia laxa), Botrytis cinerea, back bread mold (Rhizopus stolonifer) | Foliar and Fruit spraying | Spring Summer Until BBCH 87 (fruit ripe for picking) | 300–900 | 4500–13,500 g/ha 18 | ||||||||
| Grapevine (Vitis vinifera) | Downy mildew (Plasmopara viticola) | Foliar spraying | Spring Summer Until BBCH 89 stage | 1500 g/hL (Dry matter) 19 | 300–600 | 4500– 9000 g/ha 18 | |||||||
| Potato (Solanum tuberosum) | Late blight (Phytophthora infestans) | Spring Summer Until BBCH 49 (End of tuber formation) | 300–500 | 4500–7500 g/ha 18 | |||||||||
| Cucumber roots (Cucumis sativus) | Powdery mildew (Podosphaera fusca), Root fungi like common root rot, seedling blight (Pythium spp.) | Included in mulch | Not relevant | 1 | Na | Na | Na | 15 kg/ha 18 | Na | Dry plant aerial parts | |||
| Tomato (Lycopersicum esculentum) | Early blight (Alternaria solani), Septoria blight (Septoria lycopsersici) | ||||||||||||
| Ornamental trees use of which Prunus spp. Roses (Rosa spp.) | Ornamental cryptogramic diseases Rose black spot (Marsonia spp.), Rose rust (Phragmidium mucronatum), leaf curl diseases, monilioses, Oidium and mildew | ||||||||||||
| Clayed charcoal | Reg. (EU) 2017/428 Ets Christian Callegari | Grapevine (Vitis vinifera) | Protectant | Esca (black measles) caused by a complex of fungi that includes several species of Phaeoacremonium primarily by Phaeoacremonium minimum (Pm) (currently known as P. ultimum), and by Phaeomoniella chlamydospora (Pch) | Soil burying | Na | 1/3 years | 1095 | Na | Na | 500 | Na | |
| Hydrogen peroxide | Reg. (EU) 2017/409 ITAB | Vegetables—Solanaceae like tomato (Lycopersicon esculentum), bell pepper (Capsicum spp.) | Fungicide, bactericide | Soil bacteria (Ralstonia solanacerum), Botrytis cinerea | Apply before cutting | Na | To be applied before every use of the tool | Na | Na | Na | Na | Na | Waiting period 30 s after washing |
| Lettuce (Lactuca sativa) | Bacterial leaf spot pathogen (Xanthomonas campestris pv. vitians) | Seed treatment before sowing 19 | Na | 1 | Seeds are immersed in the prepared solution for 5 to 15 min (seed treatment) | ||||||||
| Horticulture flowers like common zinnia (Zinnia elegans) | Fungi, especially pathogenic Alternaria zinnia, Alternaria alternata, Fusarium spp. | ||||||||||||
| Sodium chloride | Reg. (EU) 2017/1529 Reg. (EU) 2021/556 ITAB AHDB | Grapevine (Vitis vinifera) | Fungicide, insecticide, herbicide | Fungal diseases Powdery mildews (Erysiphe necator) | Foliar application spraying | From 1st shoots (BBCH 10) to cluster tightening (BBCH 57) Spring to summer | 1–2 | Na | 600– 2000 g/hL | 200 | 1200–4000 | 30 | In case of 2 applications: one at 20 g/L + one at only 10 g/L. Maximum total rate of salt shall not exceed 6 kg/ha per year. Careful application should be controlled in terms of spray and target should be only the foliage. Low volumes are recommended in order to avoid spill. It is recommended not to spray every year, only in emergency cases. Maximum total rate of sodium chloride shall not exceed 6 kg/ha per year |
| Mushrooms like Agaricus bisporus | Fungal diseases like cobweb disease (Cladobotryum strains—i.e., Mycophilum), dry bubble disease (Lecanicillium fungicola), wet bubble disease (Mycogone perniciosa) | Hand trowel cup scoop | On finding the pathogen. No earlier than 16 days into grow cycle | 1 | Na | 0.03 g/kg | –Dry | 80–100 g/ha | Na | Salt is used as a spot treatment to cover incidents of disease. On a well-managed farm, disease will be spotted early with specialist teams identifying and spot treating. This avoids harvesters accidently spreading disease thorough contamination of personal protective equipment (PPE) and transfer to other areas. This in turn will keep on site disease levels low and avoid the use of large volumes of salt. | |||
| Grapevine (Vitis vinifera) | European grapevine moth (Lobesia botrana) | Foliar application spraying | 1st late April to May (BBCH 55–57) 2nd July (BBCH 75–77) 3rd September (BBCH 83–91) | 1–3 | Depen-ding on egg stage | 600 g/ha | 200 | 1200–3600 g/ha | 30 | Careful application should be controlled in terms of spray and target should be only the foliage. Low volumes are recommended to avoid spill. It is recommended not to spray every year, only in emergency cases | |||
| Salt swamps and salt marshes | Baccharis halimfolia | Spot application on drilled tree stump or on soil in direct vicinity of tree stump | November –February | 1 | Na | Na | Na | 10–100 g per tree stump 20 | Na | Treatment is allowed only in salt marshes and salt swamps zones as defined by national or local authorities. Treatment should be performed outside the rainy period | |||
| Beer | Reg. (EU) 2017/2090 ITAB | All edible and nonedible crops | Molluscicide | Pest slugs and snails | Specific traps for slugs | At the beginning of infestation | 1–5 | Na | Not applicable (because ready to use liquid) | Na | Na | Na | |
| Mustard seed powder | Reg. (EU) 2017/2066 ITAB | Wheat seeds (Triticum vulgare, Triticum aestivum), Durum wheat (Triticum durum), Spelt (Triticum spelta) | Fungicide for seed treatment | Fungi like Common Bunt (Tilletia caries, Tilletia foetida) | Seed application before sowing | Summer to Autumn | 1 | Na | Na | Na | 1.5 kg/100 kg seeds | Na | Mix 1.5 kg of mustard seeds powder with 4.5 L water. Treat 100 kg seeds with the slurry created |
| Talc E553B | Reg. (EU) 2018/691 COMPO Expert France SAS | Fruit trees i.e., Apple fruit (Malus Domestica), Pear tree (Pyrus sp.), Olive tree (Olea europea), etc. | Insectifuge, fungifuge | Physical barrier, Insectifuge: Insects and mites like Cacopsylla pyri, Cacopsylla fulguralis, Drosophila suzukii, Panonychus ulmi, Bactrocera oleae | Foliar application spraying | From BBCH 41 | 2–5 | 21–28 | 1st application: 2.13 to 3.54 kg/hL succeeding applications: 1.7 to 2.83 kg/hL | 600–1000 | 1st application: 21.25 kg/ha succeeding applications: 17 kg/ha | Na | Water solution prepared just before application and maintained stirred |
| Fruit trees i.e., Apple fruit (Malus Domestica), Pear tree (Pyrus sp.) | Physical barrier, Fungifuge: Foliar fungi like mildews (Venturia inaequalis, Erysiphe necator) | 3–5 | 14–21 | 1.28–2.13 kg/hL | 12.75 kg/ha | ||||||||
| Grapevine (Vitis vinifera) | From BBCH 20 | 2–5 | 21–28 | 4.25–8.5 kg/hL | 150–300 | ||||||||
| Onion oil | Reg. (EU) 2018/1295 Bionext | Carrots, celery, parsnip, parsley root | Repellent, scent masking | Carrot root fly (Psilla rosae) | Masking the smell of the umbelliferous crop by onion oil evaporated from dispensers | Shortly after planting or crop emergence (around mid–April) until end of November (before harvest) | 1 | Na | Na | Pot dispensers 0.08–0.160 L/ha Granule Dispenser 17.6–35.2 g/ha | Na | Na | 4–8 dispensers per ha professional use only |
| L-cysteine | Reg. (EU) 2020/642 Soleo-EcoSolutions | All crops and forestry in tropical areas | Insecticide | Leaf cutting ants | Hand held spreader | Post swarming (July) | 1–3 | 30 | 3–36 kg granules/ha | Na | Min 0.015 kg/ha Max 2.88 kg/ha 21 | Na | Used as an insecticide against ants. Application is made by hand on nest of ants. The application can be renewed, if necessary, with a maximum of 3 applications. Minimum/Maximum number of nests by hectare: 10–120 |
| Cow milk | Reg. (EU) 2020/1004 Basic-Eco-Logique | Grapevine (Vitis vinifera) | Fungicide and virucide | Powdery mildew (Erysiphe necator) | Foliar application Spraying | From 1st shoots (BBCH 07) to inflorescences fully developed; flowers separating (BBCH 57) 22 | 3–6 | 6–8 | 10–40 L/hL | 100–300 | 10–120 L/ha | Na | |
| Vegetable Gardening pumpkin (Cucurbita pepo) | Pumpkins powdery mildew (Podosphaera fusca) | From leaf development (BBCH 01) until flowering (BBCH 06) 23 | 3–4 | 7–12 | 50 L/hL | 400 | 200 L/ha | No application in presence of fruits | |||||
| Flower Gerbera (Gerbera jamesonii) | Powdery mildew (Erysiphe cichoracearum) | Before and during flowering (BBCH 51–69) | 3–4 | 7 | 16 L/hL | 500–1000 | 80–160 L/ha | 8 | |||||
| Cucumber (Cucumis sativus), Zucchini squash (Cucurbita pepo) | Powdery Mildew (Podosphaera fuliginea) | From three weeks after sowing (9th leaf unfolded on main stem) to 9 or more primary side shoots visible (BBCH 19–49) 24 | 3–4 | 5–10 L/hL | 1000–1500 | 50–150 L/ha | Na | ||||||
| Soybean (Glycine max (L.) Merr) | Soybean Powdery mildew (Erysiphe diffusa) | On leaves (BBCH 19–49) | 3–4 | 7 | 18 L/hL | 1000–1500 | 180–270 L/ha | ||||||
| Glove fingertips and mechanical cutting tools All crops | Viruses (mechanically transferable) e.g., Tobacco mosaic virus (TMV), Tomato mosaic virus (ToMV), Pepper mild mottle virus (PMMV), Cucumber green mottle mosaic virus (CGMMV) | Dipping | On tools | Before/after every plant contact | Before/after every plant contact | Before/after every plant contact | Na | Na | Dipping for 2 s. For reasons of efficacy use milk with at least 3,5% protein content. Replace the milk regularly (e.g., after each crop row) to prevent cross-contamination of the plants | ||||
| Allium cepa bulb extract | Reg. (EU) 2021/81 ITAB | Potatoes (Solanum tuberosum) | Fungicide | Early blight (Alternaria solani) | Spray | BBCH 21–85 | 3–5 | 7 | 1 kg/hL | 600– 1000 | 6–10 L/ha (0.3–0.5 kg onion bulb/ha) | Na | |
| Vegetable Gardening Tomato (Lycopersicum esculentum) | Tomato late blight (Phytophthora infestans) | 75 days after planting BBCH 21–75 | 3–4 | 1500 | 15 L/ha (0.75 kg onion bulb/ha) | ||||||||
| Cucumber (Cucumis sativus) | Cucumber gray mold (Botrytis cinerea) | 7 | |||||||||||
| Chitosan | Reg. (EU) 2022/456 KitoZyme | Horticulture | Fungicide | Plant elicitor, plant resistance against pathogenic fungi and bacteria | Spray Low–Medium volume spraying | BBCH 09 to BBCH 89 | 4–8 | 2 weeks | 50–100 g/hL | 200–400 | 100–400 | Na | Chitosan can be prepared for use following any of the two recipes provided in Appendix of Reg. (EU) 2022/456 (preparation for use). |
| olive trees | From 1st new leaf development BBCH 10 to development of fruit BBCH 71 | 800–3200 | |||||||||||
| grapes | 200–600 | 800–7800 | |||||||||||
| grass | BBCH 09 to BBCH 89 | 200–400 | 800–3200 | ||||||||||
| postharvest fruit treatment | Pathogenic fungi and bacteria | Immersion | Postharvest BBCH 89+ | 1 | - | 1 | - | - | |||||
1 IBA: Interval between applications; PHI: minimum preharvest interval; Na: Data not available; 2 The product cannot be applied in case of hot temperature. It is used in case of rainy period; 3 Indirect actions, no direct insecticide and fungicide properties; 4 maximum of rate per application; 5 maximum total rate per crop/season; 6 The aqueous solutions in this application are applied with few or without dilution. Here the case without dilution is calculated. Usually, not all trees are treated with brush application but only injured trees. In the calculation of maximum rate, it was assumed that 3000 trees per ha are treated with 0.15 L product per tree. This means that all trees of an orchard would be treated with several big wounds, which would be really the maximum rate and in reality, is very improbable; 7 Expressed as acetic acid. 1/1 dilution of vinegar/water L/L; 8 Considering 0.9 to 2 qt of seeds per ha; 9 Expressed as acetic acid. 50 mL/1 L dilution of vinegar/water for vinegar at 8% acetic acid; 10 Of main active substance acetic acid for vinegar at 10% acetic acid; 11 Expressed as acetic acid in a preparation with 60% vinegar (diluted in water), for vinegar at 10% acetic acid; 12 Treatments must be delayed 24–48 h or more after rain; 13 Spray when there is sun (preferably morning); 14 Do not apply when any plant is at a later growth stage than BBCH 49; 15 With a maximum of 10% concentration (30 L in 300 L); 16 Do not apply on treating fingertips right before or during harvest of edible commodities; 17 Depending upon environmental factors such as climate and topography; 18 The quantities of fresh nettle (or dry matter) written represents the quantities of nettle used in the recipe, but not the quantities that are effectively put in field—there is a filtration before; 19 Treatment, just before sowing; 20 Assuming plant density of between 0.1/m2 to 1/m2; 21 300 g of granules per nest multiplied by 120 nest/ha = 36 kg product/ha. Considering a maximum of 8% L-cysteine in the product, the maximum application rate per treatment of L-cysteine is 2.88 kg/ha; 22 Do not apply when any plant is at a later growth stage than BBCH 57; 23 Do not apply when any plant in the greenhouse is at a later growth stage than BBCH 06 and in presence of fruits; 24 Do not apply when any plant in the greenhouse is at a later growth stage than BBCH 49.
Table 2.
Typical uses of the basic substances.
| Substance Name | Use(s) | Application | Recipe | Formulation Type |
|---|---|---|---|---|
| Equisetum arv-ense | Fungicide | Spraying on crops | 2–2.25% water dilution 200 to 225 g/100 L water The product cannot be applied in case of hot tem-perature. It is used in case of rainy period | Dispersible concen-trate |
| Dry | 9 kg/100 kg mulch | Mulch | ||
| Chitosan hydrochloride | Elicitor | Spraying on crops or seeds | 0.05–0.2% water dilution 50 to 200 g/100 L water Must be applied within 24 h | Soluble powder, paste |
| Sodium hydrogen carbonate | Fungicide | Aerial parts spraying | 0.33–2% water dilution 333 to 2000 g/100 L water | Soluble powder |
| Postharvest dipping | 1–4% water dilution 1 to 4 kg/100 L water | |||
| Herbicide | Direct dusting | 10 g for a 50 cm Ø pot | Dry powder | |
| Sunflower oil | Fungicide | Foliar spraying | 0.1–0.5% water dilution 100 to 500 mL/100 L water | Oil dispersion |
| Hydrogen peroxide | Seed treatment | Seeds soaking | Ready-to-use solution (<5%) | Ready-to-use solution |
| Urtica spp. | Fungicide Insecticide | Spraying | 3–4 days maceration in water at 20 °C Fresh leaves (75 g/L) or dried leaves (15 g/L) Water dilution by 6 of filtered maceration | Dispersible concentrate |
| Mulch incorporation | Addition of dried aerial parts. 83 g/kg of mulch | Mulch | ||
| Clayed charcoal | Protectant | Soil burying | Buried. 500 kg/hectare maximum | Pellet |
| Sodium chloride | Fungicide Insecticide | Foliar spraying | 0.6–2% water dilution 600 to 2000 g/100 L water | Soluble powder |
| Substrate burying | Mix salt in the substrate. 30 g/kg substrate (3%) | Pellet | ||
| Beer | Molluscicide | Trap | Covered slug traps. 1 trap per m2 maximum | Pure product |
| Di Ammonium Phosphate | Attractant | Trap | Place in traps/bottle, 30 g/L. | Soluble powder |
| Onion oil | Odor mask | Oil dispenser | Fill the dispenser with onion oil only (20 mL) Fill the dispenser with oil then add the pellets (4.4 g oil per 30 g granule) | Oil or pellet |
| L-cysteine | Insecticide | Hand-held spreader | Mixture with matrix (flour, food grade) at a concentration of maximum 8% | Bait (ready for use) |
| Cow milk | Fungicide | Foliar spraying | 5–50% water dilution = 0.5 to 5 L of cow milk filled up with water to 10 L | Soluble concentrate |
| Dipping | Dipping tools for 2 s in undiluted cow milk. For reasons of efficacy use milk with at least 3.5% protein content | |||
| Allium cepa L. bulb extract | Fungicide | Spray application | Boil 500 g of chopped onions in 10 L of water for ten minutes then let infuse for a quarter of an hour and filter the mixture | Dispersible concentrate |
| Chitosan | Fungicide | Spray application & Immersion | Preparation 1: added to a half-filled water tank, making sure the powder is evenly distributed over the water surface to avoid aggregation. The mixture should be stirred vigorously while adding the remaining water. The mixture should be used as soon as possible. Preparation 2: dissolved in water with pH < 5. The pH of water should be regulated by adding 7 mL vinegar (8% of acetic acid) per 1 L of water). | Soluble powder |
| Vinegar | Fungicide | Seed treatment | Vinegar to be diluted in compliance with the rates of application reported in Appendix II. Undiluted for uses as herbicide on medicinal aromatic and perfume crops. For the herbicidal use in spot applications on paths, borders, sidewalks and terraces, vinegar needs to be diluted to a concentration of 60% vinegar in water (60/40 vinegar/water). | Liquid for seed treatment |
| Tools disinfection | ||||
| Herbicide | Spray or spot application/ | Liquid | ||
| pH modifier | In combination with chitosan | Liquid |
Some applications were not validated by DGSanté and Member States during discussion and votes. Some were withdrawn (Table 3) by applicants during evaluation or discussions with no regulatory trace, while some were processed up to the vote and finally non-approved with corresponding Implementing Regulations (Table 4).
Table 3.
Basic substance applications retired during the evaluation process.
| Basic Substances Removed/Withdrawn during Evaluation | |||
|---|---|---|---|
| Substance Name | Intended Use(s) | EFSA Opinion | Reason(s) |
| Castaneaand Schinopsis sp. tannins | Bactericide, fungicide and nematicide | EN 1363 | Limited number of studies about toxicity and residues led to a doubt concerning exposure assessment. Non-dietary exposure considered as hazardous |
| Honey from rhododendron | Rodenticide | EN 1155 | Lack of studies concerning substance composition and efficacy on rodents. Rodents in traps might suffer ‘too long’ |
| Extract from rhododendron | Rodenticide | EN 1596 | Lack of studies concerning substance composition and efficacy on rodents. Rodents in traps might suffer ‘too long’ |
| Quassiaamara extract | Insecticide and repellent | EN 1382 | Data gaps were identified for genotoxicity, residues, environmental risk and exposure assessment. Concerns were raised regarding reproductive and endocrine toxicity |
| Valeriana officinalis | Frost protection | None | Potential neurotoxicity, Valerian herbal tea makes it easier to fall asleep |
| Citrus pulp | - | None | - |
| Potassium metabisulfite | - | None | - |
| Didecyl-dimethylammonium chloride (DDAC) | - | RN-214 | Toxic to aquatic organisms |
Table 4.
Basic substance applications refused (non-approval).
| Substances Not Approved by the European Commission | ||||
|---|---|---|---|---|
| Substance Name | Intended Use(s) | Implementing Regulation | EFSA Opinion | Reason(s) |
| Achillea millefolium L. | Fungicide and insecticide | EU no. 2017/2057 | EN 1093 | Risk assessment for toxicology and ecotoxicology not comprehensive enough left doubts and substance is not considered as foodstuff |
| Arctium lappa L. aerial parts | Fungicide and insecticide | EU no. 2082/2015 | EN 699 | Risk assessment for toxicology and ecotoxicology not comprehensive enough left doubts and substance is not considered as foodstuff |
| Artemisia absinthium L. | Fungicide, nematicide and insecticide | EU no. 2015/2046 | EN 665 | Risk assessment for toxicology and ecotoxicology not comprehensive enough left doubts and Regulation (EC) 1334/2008 fixes limits for this substance |
| Artemisia vulgaris L. | Insecticide/repellent | EU no. 2015/1191 | EN 644 | Risk assessment for toxicology and ecotoxicology not comprehensive enough left doubts and Regulation (EC) 1334/2008 fixes limits for this substance |
| Capsicum annuum L. var. annuum, longum group, cayenne, extract (Oleoresin capsicum) | Repellent | EU no.2021/464 | EN 1838 | Risk assessment for toxicology show genotoxicity, causing serious eye damage, being harmful if swallowed and also as cause of skin irritation, although substance is considered as foodstuff |
| Caffeine | Molluscicide | EU no. 2022/xx | EN 6423 | Proposal for non-approval under discussion |
| Carbon dioxide | Rodenticide | EU no. 2021/80 | None | - |
| Comfrey steeping | Fungicide and insecticide | EU no. 2021/809 | EN 1753 | Risk assessment for toxicology and ecotoxicology not comprehensive enough left doubts and Regulation (EC) 1334/2008 fixes limits for this substance |
| Dimethyl Sulfide | Attractant | EU no. 2021/1451 | EN 1911 | Risk assessment for toxicology and ecotoxicology not provided for long-term toxicity and carcinogenicity concern |
| Grape (Vitis vinifera) cane tannins | Fungicide | EU no. 2020/29 | EN 1414 | Risk assessment for toxicology and ecotoxicology not comprehensive enough left doubts and substance is not considered as foodstuff |
| Landes pine tar | Protectant and repellent | EU no. 2018/1294 | EN 1311 | It may contain substances of concern, so there is a lack of data, so risk assessment is not comprehensive enough and left doubts |
| Origanum vulgare L. essential oil | Fungicide, bactericide and insecticide | EU no. 2017/241 | EN 1054 | Risk assessment for toxicology and ecotoxicology not comprehensive enough left doubts |
| Paprika extract E160c | Repellent | EU no. 2017/2067 | EN 1096 | Risk assessment for toxicology and ecotoxicology not comprehensive enough left doubts |
| Potassium sorbate | Fungicide | EU no. 2017/2058 | EN 1232 | Lack of data concerning residues lead to an impossibility concerning exposition assessment |
| Propolis (water soluble extract) | Fungicide and bactericide | EU no. 2020/640 | EN-1494 | Defined as a skin sensitizer, risk assessment for genotoxicity and endocrine disruption toxicity left doubts. No safe limit for the use. Substance is not considered as foodstuff |
| Rheum officinale roots extract | Fungicide | EU no. 2015/707 | EN 617 | Risk assessment for toxicology and ecotoxicology not comprehensive enough left doubts and substance is not considered as foodstuff |
| Saponaria officinalis L. roots | Acaricide and elicitor | EU no. 2020/643 | EN 1263 | Risk assessment for toxicology and ecotoxicology not comprehensive enough left doubts |
| Satureja montana L. essential oil | Fungicide and bactericide | EU no. 2017/240 | EN 1051 | Risk assessment for toxicology and ecotoxicology not comprehensive enough left doubts |
| Tanacetum vulgare L. | Repellent | EU no. 2015/2083 | EN 666 | Risk assessment for toxicology and ecotoxicology not comprehensive enough left doubts and substance is not considered as foodstuff |
| Willow bark and stem extract | Plant growth and defense elicitor | EU no.2022/ | EN 1872 | Previously proposed for non-approval since not sold for other uses, proposal under discussion, may be accepted. |
Table 5.
Examples of requests from the retailer of the amount of the Maximum Residue Level (MRL) and Acute reference doses (ARfD).
Table 5.
Examples of requests from the retailer of the amount of the Maximum Residue Level (MRL) and Acute reference doses (ARfD).
| Retailer | Max. %MRL/ Active Substance | Max. Sum %MRL/Sample | Max. %ARfD/Active Substance | Max. Sum %ARfD/Sample | Max. Number of Active Substances /Samples | |
|---|---|---|---|---|---|---|
| ALDI/ HOFER |  | 70% | 80% | 70% | 80% | 3–5 |
| ALBERT HEIJN |  | 50% | - | 50% | - | - |
| ASDA |  | 80% | - | - | - | - |
| BILLA |  | 100% | - | 100% | - | - |
| DOHLA |  | - | 70% | - | 70% | 3–5 |
| EDEKA |  | 70% | - | 100% | - | 5 |
| EDEKA OWN BRANDS | 50% | - | 70% | - | 5 | |
| GLOBUS |  | 70% | - | 70% | 100% | 5 |
| LIDL |  | 33.3% | 80% | 100% | - | 5 |
| KAUFLAND |  | 33.3% | 80% | 50% | 50% | 5 |
| NORMA |  | - | 70% | - | 70% | 5 |
| METRO |  | 50% | 80% | 70% | 100% | 5 |
| MIGROS |  | - | - | - | - | 6 |
| NETTO |  | 70% | - | 100% | - | 5 |
| REWE |  | 50% | 100% | 70% | 100% | 5 |
| REWE OWN BRANDS | 50% | 100% | 50% | - | 5 | |
| TEGUT |  | 70% | - | 70% | - | Max. 4 (>0.01 mg/kg) |
| TENGEL MANN |  | 70% | 150% | 70% | 100% | - |
Table 6.
Examples of the applications of the basic substances in research projects.
| Substance Name | Use(s) | Program | Reference |
|---|---|---|---|
| Horsetail (Equisetum arvense L.) | Fungicide | Casdar ‘4P’ Coppereplace | [24,25,26] |
| White willow bark (Salix cortex) | Fungicide | Casdar ‘4P’ | [24,25] |
| Vinegar | Seed treatment | Casdar ‘Carie’ | [27]; http://itab.asso.fr/programmes/carie-ble.php, accessed on 23 May 2022. |
| Mustard seed powder | Seed treatment | ||
| Sucrose | Elicitor | Ecophyto ‘Usage’ and Casdar ‘Sweet’, ABAPIC | [28]; https://ecophytopic.fr/cuivre-viticulture/proteger/micro-doses-de-sucre, accessed on 23 May 2022. [29]; https://ecophytopic.fr/sites/default/files/USAGE.pdf, accessed on 23 May 2022. |
| Fructose | Elicitor | ||
| Lecithin | Fungicide | Casdar ‘HE’ | [30]; https://ecophytopic.fr/recherche-innovation/proteger/projet-he, accessed on 23 May 2022. |
| Talc | Fungicide | out of program | [31] |
| Whey | Fungicide | [32] | |
| Di-ammonium phosphate (DAP) | Attractant | [33]; https://ecophytopic.fr/pic/proteger/proteger-ses-oliviers-de-la-mouches-en-limitant-les-traitements, accessed on 23 May 2022. | |
| Calcium hydroxide | Fungicide | [34]; https://www.researchgate.net/publication/279636728_The_post-infection_activity_of_hydrated_lime_against_conidia_of_Venturia_inaequalis, accessed on 23 May 2022. | |
| Chitosan hydrochloride | Fungicide | Vitinnova | [35]; www.vitinnova.it/en, accessed on 23 May 2022. |
| Euphresco BasicS | [16]; https://www.researchgate.net/project/EUPHRESCO-Basic-substances-as-an-environmentally-friendly-alternative-to-synthetic-pesticides-for-plant-protection-BasicS, accessed on 23 May 2022. | ||
| PRIMA StopMedWaste | [36]; www.stopmedwaste.eu, accessed on 23 May 2022. | ||
| ZeroSprechi | [37]; www.zerosprechi.info/en/zerosprechi, accessed on 23 May 2022. | ||
| CleanSeed | [38]; https://www.cleanseed.it/en/cleanseed-2/, accessed on 23 May 2022. |
Each use of plant extracts and natural products, such as decoctions, herbal teas, or aqueous solutions, have been defined and tested in the field or identified from the literature then controlled or cross-referenced with producer surveys. Whenever water is mentioned in these tests, it is either natural spring water or rainwater. Each basic substance preparation is described in Section 2.5 of Basic substances applications in EU 2012. The evaluation process of the basic substance application is getting longer, and legal delays fixed by EC are not consistently respected. The evaluation process lasts an average of 19 months (Supplementary Table S1 and Figure S1), while the legal maximum delay is fixed at 18 months until basic substance application admissibility. Even not considering admissibility evaluation delays that are considered outside of the evaluation process, this process becomes longer from year to year, resulting in a delay in availability of additional basic substances.
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Romanazzi, G.; Orçonneau, Y.; Moumni, M.; Davillerd, Y.; Marchand, P.A. Basic Substances, a Sustainable Tool to Complement and Eventually Replace Synthetic Pesticides in the Management of Pre and Postharvest Diseases: Reviewed Instructions for Users. Molecules 2022, 27, 3484. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27113484
AMA Style
Romanazzi G, Orçonneau Y, Moumni M, Davillerd Y, Marchand PA. Basic Substances, a Sustainable Tool to Complement and Eventually Replace Synthetic Pesticides in the Management of Pre and Postharvest Diseases: Reviewed Instructions for Users. Molecules. 2022; 27(11):3484. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27113484
Chicago/Turabian StyleRomanazzi, Gianfranco, Yann Orçonneau, Marwa Moumni, Yann Davillerd, and Patrice André Marchand. 2022. "Basic Substances, a Sustainable Tool to Complement and Eventually Replace Synthetic Pesticides in the Management of Pre and Postharvest Diseases: Reviewed Instructions for Users" Molecules 27, no. 11: 3484. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27113484
