Combination Therapy of Carnosic Acid and Methotrexate Effectively Suppressed the Inflammatory Markers and Oxidative Stress in Experimental Arthritis
Abstract
:1. Introduction
2. Results
2.1. Change in Body Weight
2.2. Hind Paw Volume
2.3. Plasmatic Interleukin-17A
2.4. Matrix Metalloproteinase-9 in Plasma
2.5. Monocyte Chemotactic Protein-1 in Plasma
2.6. Activity of Gamma-Glutamyl Transferase in Joint
2.7. Relative mRNA Expression of Interleukin-1β and Antioxidant Enzymes Heme Oxygenase (HO-1) and Catalase (CAT)
3. Discussion
4. Materials and Methods
4.1. Experimental Animals
4.2. Induction of Adjuvant Arthritis in Lewis Rats
4.3. Experimental Design and Treatment
4.4. Change in Body Weight
4.5. Hind Paws’ Volume Change
4.6. Markers of Inflammation
4.7. The Activity of the Gamma-Glutamyltransferase in Joint
4.8. RNA Extraction and Reverse Transcription
4.9. Quantitative Real-Time RT-PCR
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group | Mean |
---|---|
HC | 11.46 ± 1.39 |
AA | 20.94 ± 2.01 + |
MTX | 12.48 ± 2.59 |
CA | 17.88 ± 1.00 |
CA-MTX | 10.95 ± 1.40 * |
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Chrastina, M.; Poništ, S.; Tóth, J.; Czigle, S.; Pašková, Ľ.; Vyletelová, V.; Švík, K.; Bauerová, K. Combination Therapy of Carnosic Acid and Methotrexate Effectively Suppressed the Inflammatory Markers and Oxidative Stress in Experimental Arthritis. Molecules 2022, 27, 7115. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27207115
Chrastina M, Poništ S, Tóth J, Czigle S, Pašková Ľ, Vyletelová V, Švík K, Bauerová K. Combination Therapy of Carnosic Acid and Methotrexate Effectively Suppressed the Inflammatory Markers and Oxidative Stress in Experimental Arthritis. Molecules. 2022; 27(20):7115. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27207115
Chicago/Turabian StyleChrastina, Martin, Silvester Poništ, Jaroslav Tóth, Szilvia Czigle, Ľudmila Pašková, Veronika Vyletelová, Karol Švík, and Katarína Bauerová. 2022. "Combination Therapy of Carnosic Acid and Methotrexate Effectively Suppressed the Inflammatory Markers and Oxidative Stress in Experimental Arthritis" Molecules 27, no. 20: 7115. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27207115