Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity
Abstract
:1. Introduction
2. Results
2.1. Amber Extract Protects SHSY5Y Cells against 6-OHDA-Induced Cytotoxicity
2.2. Amber Extract Decreases 6-OHDA-Induced Apoptosis in SHSY5Y Cells
2.3. Amber Extract Decreases 6-OHDA-Induced ROS Generation in SHSY5Y Cells
2.4. Effect of Amber Extract on Autophagy-Related Gene Expression in SHSY5Y Cells
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Amber Extract
4.3. Cell Culture
4.4. MTT Assay
4.5. Apoptosis Assay
4.6. ROS Assay
4.7. Western Blotting
4.8. Data Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Luo, Y.; Zhou, S.; Takeda, R.; Okazaki, K.; Sekita, M.; Sakamoto, K. Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity. Molecules 2022, 27, 1817. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27061817
Luo Y, Zhou S, Takeda R, Okazaki K, Sekita M, Sakamoto K. Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity. Molecules. 2022; 27(6):1817. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27061817
Chicago/Turabian StyleLuo, Yuening, Siqi Zhou, Reiko Takeda, Kazuma Okazaki, Marie Sekita, and Kazuichi Sakamoto. 2022. "Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity" Molecules 27, no. 6: 1817. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27061817