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Article

Enhanced Enzymatic Performance of Immobilized Pseudomonas fluorescens Lipase on ZIF-8@ZIF-67 and Its Application to the Synthesis of Neryl Acetate with Transesterification Reaction

School of Chemistry and Chemical Engineering, Henan University of Science and Technology, Luoyang 471023, China
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Author to whom correspondence should be addressed.
Submission received: 28 May 2024 / Revised: 16 June 2024 / Accepted: 18 June 2024 / Published: 19 June 2024

Abstract

In this study, hybrid skeleton material ZIF-8@ZIF-67 was synthesized by the epitaxial growth method and then was utilized as a carrier for encapsulating Pseudomonas fluorescens lipase (PFL) through the co-precipitation method, resulting in the preparation of immobilized lipase (PFL@ZIF-8@ZIF-67). Subsequently, it was further treated with glutaraldehyde to improve protein immobilization yield. Under optimal immobilization conditions, the specific hydrolytic activity of PFL@ZIF-8@ZIF-67 was 20.4 times higher than that of the free PFL. The prepared biocatalyst was characterized and analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared (FT-IR). Additionally, the thermal stability of PFL@ZIF-8@ZIF-67 at 50 °C was significantly improved compared to the free PFL. After 7 weeks at room temperature, PFL@ZIF-8@ZIF-67 retained 78% of the transesterification activity, while the free enzyme was only 29%. Finally, PFL@ZIF-8@ZIF-67 was applied to the neryl acetate preparation in a solvent-free system, and the yield of neryl acetate reached 99% after 3 h of reaction. After 10 repetitions, the yields of neryl acetate catalyzed by PFL@ZIF-8@ZIF-67 and the free PFL were 80% and 43%, respectively.
Keywords: lipase; ZIF-8@ZIF-67; immobilization; neryl acetate; transesterification reaction lipase; ZIF-8@ZIF-67; immobilization; neryl acetate; transesterification reaction

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MDPI and ACS Style

Wang, Q.; Xiong, J.; Xu, H.; Sun, W.; Pan, X.; Cui, S.; Lv, S.; Zhang, Y. Enhanced Enzymatic Performance of Immobilized Pseudomonas fluorescens Lipase on ZIF-8@ZIF-67 and Its Application to the Synthesis of Neryl Acetate with Transesterification Reaction. Molecules 2024, 29, 2922. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules29122922

AMA Style

Wang Q, Xiong J, Xu H, Sun W, Pan X, Cui S, Lv S, Zhang Y. Enhanced Enzymatic Performance of Immobilized Pseudomonas fluorescens Lipase on ZIF-8@ZIF-67 and Its Application to the Synthesis of Neryl Acetate with Transesterification Reaction. Molecules. 2024; 29(12):2922. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules29122922

Chicago/Turabian Style

Wang, Qi, Jian Xiong, Hanghang Xu, Wenyuan Sun, Xiaoxu Pan, Shixin Cui, Siting Lv, and Yinling Zhang. 2024. "Enhanced Enzymatic Performance of Immobilized Pseudomonas fluorescens Lipase on ZIF-8@ZIF-67 and Its Application to the Synthesis of Neryl Acetate with Transesterification Reaction" Molecules 29, no. 12: 2922. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules29122922

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