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Article

An Adenovirus Vector Expressing FMDV RNA Polymerase Combined with a Chimeric VLP Harboring a Neutralizing Epitope as a Prime Boost Strategy to Induce FMDV-Specific Humoral and Cellular Responses

1
Centro de Investigación en Sanidad Animal (INIA, CSIC), Ctra de Algete a El Casar de Talamanca, Valdeolmos, 28130 Madrid, Spain
2
Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICA-SAT-AIP), City of Knowledge, Panama 0843-01103, Panama
*
Author to whom correspondence should be addressed.
Academic Editors: Juan Carlos Saiz and Alfredo Berzal-Herranz
Pharmaceuticals 2021, 14(7), 675; https://0-doi-org.brum.beds.ac.uk/10.3390/ph14070675
Received: 20 June 2021 / Revised: 6 July 2021 / Accepted: 12 July 2021 / Published: 15 July 2021
(This article belongs to the Special Issue Current Trends in RNA Virus Vaccines)
Foot and mouth disease is a highly contagious disease affecting cattle, sheep, and swine among other cloven-hoofed animals that imposes serious economic burden by its direct effects on farm productivity as well as on commerce of farmed produce. Vaccination using inactivated viral strains of the different serotypes is an effective protective measure, but has several drawbacks including a lack of cross protection and the perils associated with the large-scale growth of infectious virus. We have previously developed chimeric virus-like particles (VLPs) bearing an FMDV epitope which induced strong specific humoral responses in vaccinated pigs but conferred only partial protection against homologous challenge. While this and other FMD vaccines under development mostly rely on the induction of neutralizing responses, it is thought that induction of specific T-cell responses might improve both cross protective efficacy as well as duration of immunity. Therefore, we here describe the development of a recombinant adenovirus expressing the highly conserved nonstructural FMDV 3D protein as well as its capacity to induce specific T-cell responses in a murine model. We further describe the generation of an FMDV serotype C-specific chimeric VLP and analyze the immunogenicity of two different prime-boost strategies combining both elements in mice. This combination can effectively induce both humoral and cellular FMDV-specific responses eliciting high titers of ELISA and neutralizing antibodies anti-FMDV as well as a high frequency of IFNγ-secreting cells. These results provide the basis for further testing of this anti FMD vaccination strategy in cattle or pig, two of the most relevant natural host of this pathogen. View Full-Text
Keywords: foot and mouth disease virus; virus-like particle; adenovirus vaccine; prime-boost vaccination foot and mouth disease virus; virus-like particle; adenovirus vaccine; prime-boost vaccination
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MDPI and ACS Style

Rangel, G.; Martín, V.; Bárcena, J.; Blanco, E.; Alejo, A. An Adenovirus Vector Expressing FMDV RNA Polymerase Combined with a Chimeric VLP Harboring a Neutralizing Epitope as a Prime Boost Strategy to Induce FMDV-Specific Humoral and Cellular Responses. Pharmaceuticals 2021, 14, 675. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14070675

AMA Style

Rangel G, Martín V, Bárcena J, Blanco E, Alejo A. An Adenovirus Vector Expressing FMDV RNA Polymerase Combined with a Chimeric VLP Harboring a Neutralizing Epitope as a Prime Boost Strategy to Induce FMDV-Specific Humoral and Cellular Responses. Pharmaceuticals. 2021; 14(7):675. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14070675

Chicago/Turabian Style

Rangel, Giselle, Verónica Martín, Juan Bárcena, Esther Blanco, and Alí Alejo. 2021. "An Adenovirus Vector Expressing FMDV RNA Polymerase Combined with a Chimeric VLP Harboring a Neutralizing Epitope as a Prime Boost Strategy to Induce FMDV-Specific Humoral and Cellular Responses" Pharmaceuticals 14, no. 7: 675. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14070675

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