Characterization of Complete Mitochondrial Genome and Phylogenetic Analysis of a Nocturnal Wasps—Provespa barthelemyi (Hymenoptera: Vespidae)
Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsThe authors of this paper sequenced the full mitochondrial genome of a nocturnal wasps as well as performed phylogenetic analysis of obtained the position of nocturnal wasp of the Vespidae family.
I have some comments to improve the paper.
I suggest including in the title Phylogenetic analysis.
Line 33-34. This sentence seems to need additional text.
Line 37. Difference – visible difference.
Line 39-40. Can the authors hypothesize the main reasons for the transition from a solitary to a social life?
Line 46. ribosome DNA – replace with nuclear 28S ribosomal DNA.
Line 63. 2.1. Taking Samples and DNA Isolation – Sampling and DNA Isolation.
Line 67. Rstored - rеstored.
Line 69. Please, mention the manufacturer.
Line 73. Cat. no., city, country?
Line 83. Please, add the following sentence after that. The sequenced mitochondrial of the P. barthelemyi was deposited in GenBank under Acc. no……
Line 86-87. Please, cite the following paper:
M. Bernt, A. Donath, F. Jühling, F. Externbrink, C. Florentz, G. Fritzsch, J. Pütz, M. Middendorf, P. F. Stadler. MITOS: Improved de novo Metazoan Mitochondrial Genome Annotation Molecular Phylogenetics and Evolution 2013, 69(2):313-319
Line 89. SnapGene Viewer – Please, add a web link to this and others web-based programs!
Line 124. by sequencing the Vespidae - clarify which gene or whole genome was sequenced?
Line. 135. ND1 – nad1.
Line 142. codon usage of 376, 360, 342 – 376 bp and so on.
Line 148. P. barthelemyi - P. barthelemyi.
Line 151-152. Any citations to support that?
Line 153. Parapolybia crocea and Orancistrocerus aterrimus aterrimus – put in italic.
Line 162. nad4l – nad4?
Line 174. 19 reported – available in GenBank database.
Line 200. Please, add Acc.no. of the newly sequenced mitochondrial genome of the P. barthelemyi!
Comments on the Quality of English LanguageMinor editing of English language required.
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for AuthorsThis is an interesting article reporting on the characterization of complete mitochondrial genome of a nocturnal wasps - Provespa barthelemyi (Hymenoptera: Vespidae). The Authors used Maximum Likelihood (ML) methodologies to construct a phylogenetic tree based on the sequenced mitochondrial genome and the available data of reported species belonging to Vespinae, confirming the monophyly of Vespinae. They also reported the first complete mitochondrial genome of Provespa and compared its characteristics with other mitochondrial genomes in the family Vespidae. The results obtained contribute to improve the knowledge on the phylogenetic relationships and ecological characters of the Vespidae family.
Introduction is adequate.
M&M is clear and detailed.
Results and Discussion are appropriate and supported by tables and figures.
Conclusions is in line with Results and Discussion.
Refrences are appropriate.
Supplementary Materials: they are mentioned in the specific section but not cited in the text. In my opinion, their citation should be added.
Investigate the characterization of complete mitochondrial genome of a nocturnal wasps - Provespa barthelemyi (Hymenoptera: Vespidae).
The results obtained contribute to improve the knowledge on the phylogenetic relationships and ecological characters of the Vespidae family.
The first complete mitochondrial genome of Provespa is provided.
This is the first study providing the complete mitochondrial genome of Provespa.
The methodology is already appropriate.
As already said the conclusions are in accordance with the results and discussion of the article.
Tables and figures included in the article are clear and do not require further information.
Minor changes are indicated in the manuscript.
Comments for author File: Comments.pdf
In my opinion, minor editing of English language is required.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
