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Article

The Prognostic Utility of KRAS Mutations in Tissue and Circulating Tumour DNA in Colorectal Cancer Patients

by
Joel Petit
1,2,3,*,
Georgia Carroll
1,3,4,
Jie Zhao
1,
Peter Pockney
2,3 and
Rodney J. Scott
2,3,5
1
Division of Surgery, John Hunter Hospital, New Lambton Heights, NSW 2305, Australia
2
School of Biomedical Sciences and Pharmacy, University of Newcastle, New Lambton Heights, NSW 2305, Australia
3
Hunter Medical Research Institute, University of Newcastle, New Lambton Heights, NSW 2305, Australia
4
School of Medicine and Public Health, University of Newcastle, New Lambton Heights, NSW 2305, Australia
5
NSW Health Pathology, New Lambton Heights, NSW 2305, Australia
*
Author to whom correspondence should be addressed.
Gastroenterol. Insights 2024, 15(1), 107-121; https://0-doi-org.brum.beds.ac.uk/10.3390/gastroent15010008
Submission received: 20 November 2023 / Revised: 12 January 2024 / Accepted: 22 January 2024 / Published: 27 January 2024
(This article belongs to the Section Gastrointestinal Disease)
Browse Figures
Versions Notes

Abstract

:
This study aims to investigate the long-term prognostic utility of circulating tumour DNA (ctDNA) KRAS mutations in colorectal cancer (CRC) patients and compare this with KRAS mutations in matched tissue samples. Tumour tissue (n = 107) and ctDNA (n = 80) were obtained from patients undergoing CRC resection and were analysed for KRAS mutations. The associations between KRAS mutation and overall survival (OS), cancer-specific survival (CSS), and recurrence-free survival (RFS) were analysed. All outcomes were measured in years (y). A total of 28.8% of patients had KRAS mutations in ctDNA and 72.9% in tumour tissue DNA. The high frequency of KRAS mutations in tissue samples was due to 51.4% of these being a detectable low mutation allele frequency (<10% MAF). Comparing KRAS mutant (KRASmut) to KRAS wild-type (KRASwt) in ctDNA, there was no association found with OS (mean 4.67 y vs. 4.34 y, p = 0.832), CSS (mean 4.72 y vs. 4.49 y, p = 0.747), or RFS (mean 3.89 y vs. 4.26 y, p = 0.616). Similarly, comparing KRASmut to KRASwt in tissue DNA there was no association found with OS (mean 4.23 y vs. 4.61 y, p = 0.193), CSS (mean 4.41 y vs. 4.71 y, p = 0.312), or RFS (mean 4.16 y vs. 4.41 y, p = 0.443). There was no significant association found between KRAS mutations in either tissue or ctDNA and OS, CSS, or RFS.

1. Introduction

Colorectal cancer (CRC) was responsible for an estimated 862,000 deaths globally in 2018 which makes it the second leading cause of cancer-related death [1]. Advances in treatment and early detection strategies over the last decade have significantly changed the medical management of this disease and improved overall survival. However, the outcomes for CRC patients remain closely related to the stage of cancer at diagnosis. The 5 year survival rate of patients is inversely related to the stage of the disease. At stage I, there is a 99% survival rate, at stage II it is 89%, at stage III it is 71%, and at stage IV it drops precipitously to 13% [2]. Whilst most patients with stage II or III disease have good outcomes, there is a proportion of patients who are affected by disease recurrence. It has been shown that there is a survival benefit to offering adjuvant chemotherapy to patients with stage III disease; however, this benefit has not been seen in patients with stage II disease [3]. Despite the absence of benefit in patients with stage II disease, there may be a subgroup who would benefit if they were able to be identified by appropriate biomarkers after surgical resection with curative intent. To date, it has not been possible to identify which patients with stage II or III disease will develop recurrence. However, recent evidence from Tie et al., who used sequencing to identify multiple mutations from primary tumour tissue and plasma ctDNA that subsequently guides therapeutic choices in stage II colon cancer patients, has shown non-inferiority to standard management [4]. By using a ctDNA-guided approach there was a reduced use of adjuvant chemotherapy without a significant compromise in recurrence-free survival (RFS).
The potential of using KRAS mutations in primary tumour samples as a prognostic or predictive marker has been proposed by many researchers. Early studies suggested that KRAS mutations could be used as a prognostic marker; however, there have been conflicting results from more recent studies [5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30]. Almost half the research in this field has found no significant association between KRAS mutation status and prognostic outcomes of OS, CSS, or RFS. During the last few years, the research in this area has become even more complicated since more recent studies have replaced tissue tumour samples with ctDNA analysis and the concordance of mutational status between tumour tissue samples and ctDNA varies widely between studies. Most of these studies on tissue and ctDNA have been small, with around 100 patients. KRAS mutations found in these different types of samples could have diverse prognostic or predictive utility and caution should be used when comparing results from these studies. This study aims to add to the evidence regarding possible utility of the long-term prognostic ability of ctDNA KRAS mutation detection in colorectal cancer patients and compare this with KRAS mutations in matched tissue samples.

2. Materials and Methods

2.1. Sample Collection and Ethics

Specimens were collected from consecutive patients undergoing a CRC resection between July 2011 and December 2013 at either the John Hunter Hospital or Newcastle Private Hospital. The tissue samples were collected from the primary tumour at the time of resection prior to fixation and snap frozen and stored at −80 °C. The blood samples were collected in either K2-EDTA tubes or lithium heparin tubes pre-operatively and were processed within 4 h of phlebotomy. Two centrifugation steps were used to prepare plasma samples, which were then stored at −80 °C (Supplementary S1). For the purposes of this study, a total of 121 patients had suitable specimens that were able to be utilised for either plasma (n = 80) or tissue (n = 107) analysis. A total of 66 patients had both tissue and plasma available for analysis. Histopathological examination and status of the tumour was confirmed by a certified pathologist and staged using the TNM system defined by the Union for International Cancer Control (UICC) [31]. Collected patient characteristics included age, gender, body mass index (BMI), smoking status, comorbidities graded as the Charlson Co-morbidity Index (CCI), site of tumour, histopathology, tumour staging, use of adjuvant therapy, disease recurrence, and mortality. This study was conducted in accordance with the Helsinki Declaration and was approved by the Hunter New England Human Research and Ethics Committee (2019/ETH01147, 2019/ETH10205). Patient consent for specimen collection and analysis was obtained prior to their procedures in all cases.

2.2. DNA Extraction

A standard ethanol and salt extraction method was used to isolate genomic DNA from the fresh frozen tissue samples (Supplementary S1). The genomic tissue DNA was then prepared for droplet digital polymerase chain reaction (ddPCR) using Zymo DNA Clean and Concentrator kits (Zymo Research, Irvine, CA, USA). Purification of DNA from plasma was performed using Zymo Quick-cfDNA Serum and Plasma kits. The total amount of genomic DNA purified from the plasma samples was quantified using Qubit 2.0, dsDNA high-sensitivity assay (Life Technologies, Carlsbad, CA, USA). All methods were performed according to the manufacturer’s instructions.

2.3. KRAS-Mutation Testing Using ddPCR

Genomic DNA extracted from plasma and tissue were analysed for 7 KRAS mutations (G12A, G12C, G12D, G12R, G12S, G12V, and G13D) by ddPCR using the KRAS G12/G13 Screening kit (Bio-rad, Hercules, CA, USA). KRAS multiplex analysis was performed using 1–8 µL volume of sample DNA. KRAS master mixes were made for all different volumes used in each run. For each reaction, the master mix contained 1.1 µL of KRAS primer/probe mix, 11 µL of ddPCR Supermix (Bio-rad, Hercules, CA, USA), and autoclaved Millipore water in variable volumes relative to the sample input volume. The sample and master mix were combined to achieve a total end volume of each PCR reaction of 22 µL. The 96-well plate was then sealed, centrifuged at 300 rpm for 5 s, gently vortexed, and recentrifuged at 300 rpm. The plate seal was removed, and the plate was then run on the QX200 AutoDG Droplet Digital PCR system, immediately foil heat sealed using the PX1 PCR Plate Sealer, and run on the C1000 Touch Thermocycler. The PCR cycling conditions were as per the manufacturer’s instructions. The plate was then placed into the QX200 Droplet Reader for analysis, and the data were analysed using QuantaSoft software v1.2 (Bio-rad, Hercules, CA, USA). For each PCR plate, there were control samples for both mutation and wild-type KRAS that were made using Horizon reference standards. All assays included a no template control (NTC).

2.4. Calculation of the LoD and LoB

The limit of detection (LoD) and limit of blank (LoB) for the ddPCR analysis were measured according to the methods described by Armbruster et al. [32]. The LoB was determined by 22 replicates of PCR wells that contained only KRAS wild-type controls. The LoD was determined by the following equation:
LoD = LoB + 1.645(SDblank)
The LoD using this method was 4.73 droplets. The cut-off for a positive KRAS mutation call was, therefore, conservatively placed at 6 or more positive droplets. Alternate cut-offs of more than 1%, 5%, and 10% mutation allele frequency (MAF) were also used for the tissue cohort in separate analyses.

2.5. Statistical Analysis

Overall survival (OS) was defined as the time from the cancer operation until death from any cause. Cancer-specific survival (CSS) was defined as the time from the cancer operation until death from colorectal cancer. Patients were excluded from OS and CSS analysis if they had metastatic disease that did not undergo an R0 resection plus metastasectomy. Recurrence-free survival (RFS) was defined as the time from the cancer operation until the first objective evidence of disease progression or death from colorectal cancer. Patients were excluded from RFS analysis if they survived < 30 days from the initial operation or had metastatic disease that did not undergo an R0 resection plus metastasectomy. The survival and recurrence of patients in each KRAS mutation group were examined using Kaplan–Meier curves and stratified by two potential prognostic factors: KRAS mutation in tissue and cell-free KRAS DNA. The analysis was performed using both total available survival data for each individual as well as survival data adjusted to an endpoint of 5 years.
Associations between the presence of a KRAS mutation and patient survival were examined using Cox regression. For all three survival outcomes, the non-adjusted (crude) effect of the presence of KRAS (in tissue as well as cell-free DNA) was examined for association. For OS and RFS, the effect of the KRAS mutations after adjustment for potential confounders was examined for association. The CCI had the most impact on results during multivariate analysis; hence, the adjusted variables presented utilise this confounder. Cox regression model estimates are presented as estimate hazard ratios (HRs) with 95% confidence intervals (CIs).
The proportional hazard assumptions were assessed by visual inspection of Kaplan–Meier curves assessing time-dependent covariates and plotting simulated cumulative Martingale residuals of each covariate. The assumption of proportional hazards was deemed appropriate. Statistical analyses were programmed using SPSS v28. A priori, p < 0.05 (two-tailed) was used to indicate statistical significance.

3. Results

3.1. KRAS Mutation in ctDNA and Prognosis

Among the 121 patients in this study, there was insufficient plasma for analysis of 41 patients. Insufficient plasma occurred due to the use of plasma samples in another prior study. One further patient had sufficient ctDNA but had incomplete follow-up data so was also excluded. Among the remaining 80 patients, 23 were positive for KRAS mutation in ctDNA (28.8%). Patient demographic data and clinical characteristics by ctDNA KRAS status are shown in Table 1. The frequency of KRAS mutation was found to be significantly higher in non-smokers and those who received adjuvant chemotherapy. All other characteristics were not significantly associated with the KRAS mutation status. Interestingly, well and moderately differentiated tumours were the most predominant tumour grade in both KRAS negative and KRAS positive cases; however, the frequency of poorly differentiated tumours was three times higher in KRAS positive cases. Despite these differences, our analysis did not reveal a significant association between KRAS status and tumour grade. There was no significant association found between OS, CSS, or RFS in patients who tested positive for ctDNA KRASmut compared to those who were KRASwt (Table 2 and Table 3, Figure 1, Figure 2 and Figure 3). Adjustment for potential confounders produced no statistically significant changes.

3.2. KRAS Mutation in Tumour Tissue and Prognosis

Tumour tissue was available for analysis from 107 patients. Amongst these patients, 78 were positive for KRAS mutation in tumour tissue DNA when using the LoD cut-off (72.9%). This rate decreased sequentially with cut-off values of 1%, 5%, or 10% MAF, which resulted in frequencies of 24.3%, 22.4%, and 21.5%, respectively. Patient demographic data and clinical characteristics by tissue KRAS status are shown in Table 4. The frequency of KRAS mutation was not significantly different in any of these factors. There was no significant association found between OS, CSS, or RFS in patients who tested positive for tumour tissue DNA KRASmut compared to those who were KRASwt (Table 5, Figure 4, Figure 5 and Figure 6). However, there was a trend towards decreased overall, cancer-specific, and recurrence-free survival for tumour tissue KRASmut positive cases. The same non-statistically significant trend was seen when analysis was performed using a >10 MAF cut-off point (Table 6). Similarly, although it is not consistent across every model, there was a trend towards an increased HR for all these outcomes (Table 7). Adjustment for confounders produced no statistically significant changes.

4. Discussion

The results show a trend towards increased risk of disease recurrence and decreased OS and CSS for patients with KRAS mutation found in their tumour tissue. However, these trends were not statistically significant in univariate or multivariate analyses. It is possible that this study was underpowered; however, as previously demonstrated, the number of participants with tissue samples in this study (n = 107) was above the median number of participants (n = 97) found after a review of the literature when searching for articles analysing the prognostic utility of KRAS mutations in CRC tumour tissue or plasma (Chapter 1: Background—Table 1). Regarding features such as the TNM stages included the following: receipt of adjuvant chemotherapy, type of chemotherapy received, type of PCR method used, and methods of survival analysis; the heterogeneity of the research found illustrates the difficulty in comparing the current evidence in this field.
Our results show a non-significant trend towards an increased risk of disease recurrence, a decreased overall survival, and a decreased cancer-specific survival for patients with KRAS mutation-positive tissue at the time of diagnosis and initial treatment. These results were not reiterated in ctDNA, which failed to show any trend or significant association between KRAS mutation status and overall survival, cancer-specific survival, or recurrence-free survival.
The number of patients found to have KRASmut-positive tissue samples was well above the expected range of 20% to 40% [33]. However, if the MAF cut-offs of 1%, 5%, and 10% are used, then the frequency falls within the expected range of 21–25%. Despite the higher KRASmut clonal population of cells present in the tumours with these cut-offs, this did not translate into any significant changes in survival or recurrence (Table 6 and Table 7).
The limitations of this study are its relatively small sample size, retrospective nature, and lack of complete standardization of patient care and plasma collection methods. The patients were managed clinically at the discretion of the treating surgeon and oncologist, and CEA was not routinely performed for all patients, hence there were insufficient numbers to include this marker in the analysis. The slight differences in the collection of the plasma samples potentially could have affected the overall frequency of ctDNA KRAS mutations. However, since the classification of KRAS mutation status was based on the LoD, this should be unaffected by potential variances in cell-free DNA release at the time of collection due to different sample types. Furthermore, ddPCR has been found to have a high resistance to the effect of potential PCR inhibitors, and Sefrioui et al. found that heparinase treatment of samples did not quantitatively or qualitatively alter the ctDNA detection [34,35,36]. Finally, although the sample size is small, it is comparable to other studies in this area and there is a significantly longer follow-up time for this cohort. The sample size was insufficient to perform subgroup analyses for the KRAS status stratified by either adjuvant therapy, site of cancer, or resection margins, which are all known to have effects on oncological outcomes.
Similarly, the sample size was insufficient to perform subgroup analyses based on the specific KRAS mutation type. This is in addition to the fact that the specific assay kit used was not able to sufficiently differentiate the mutation types to allow for certainty. There is mixed evidence regarding the effect that specific codon mutations have on prognoses. Jones et al. and Imamura et al. found that the G12C and G12V mutations conferred a worse prognosis compared to other mutations in codons 12, 13, or 61 [37,38]. However, a pooled analysis of patients from five trials found conflicting results that suggested there was a similarly poorer prognosis with G12C mutations but that G12D and G12V mutations had no obvious impacts on OS in univariate and multivariate analyses [39]. These studies were all performed on tissue samples, which highlights that there is still ongoing debate about the prognostic utility of KRAS mutations for CRC even when the mutational analysis is performed on the primary cancer itself. Studies that have found an association between KRAS mutations and OS are more likely to have recruited patients with metastatic colorectal cancer rather than early-stage cancer. For instance, Mendoza-Moreno et al. found that at 36 months more patients with peritoneal metastases and KRASwt tumours were alive compared to KRASmut (31% vs. 15%; p < 0.001) [40]. Alkader et al. found a similar association of shorter overall survival in KRASmut when compared to KRASwt patients (21 months vs. 17 months) [41].
The poor concordance (44%) between ctDNA and tumour tissue KRAS mutation status in matched patient samples suggests the limited feasibility of ctDNA as a useful biomarker (Table 8). However, the situation is complex and the reason for this discordance is potentially multifactorial. Firstly, the accuracy and sensitivity of ctDNA seem to increase with the overall burden of disease. Several small and large studies have found that the diagnostic accuracy of ctDNA is related to the stage of disease, and, therefore, whilst early-stage tumours could harbor KRAS mutations, these are less likely to be simultaneously found in ctDNA when compared to stage III or IV cancers [20,42,43,44]. This pattern explains why many of the studies utilising ctDNA focused only on metastatic CRC [22,25,26,27,28,29]. Furthermore, the heterogeneity of genetic mutations within CRC means that in many of the tissue DNA samples, there is likely to be a small sub-clonal population of cells with KRAS mutations. This fractional volume of KRAS mutated cancer cells may not shed enough DNA into the circulation to produce a positive result in the plasma whilst still being sufficient to be detected at low volumes in the tumour tissue due to the high sensitivity of ddPCR. This is supported by the fact that the frequency of KRAS mutations drops from 72.9% to 21.5% if a cut-off of an MAF > 10% is used rather than the LoD methodology. Similarly, the concordance between ctDNA and tumour tissue also increases from 44% to 73% with this change in cut-off value.
Although this study is not designed to answer any questions regarding the clinical significance of the detection of low-volume KRAS mutations, it is likely that this is related to the emergence of acquired resistance to targeted epidermal growth factor receptor (EGFR) blockade therapy [45,46,47,48]. The use of anti-EGFR therapy in CRC is limited to patients with a KRAS mutation-negative status (generally a MAF < 5% on tumour tissue) since it has been shown that this treatment has a reduced effect on OS, CSS, or RFS in patients harbouring a KRAS mutation [49]. However, in this cohort, around 50% of the cases were found to harbour low-frequency KRAS mutations that would lead to the development of treatment resistance. There is evidence that the emergence of resistance to anti-EGFR treatment can be overcome if combined with other therapies simultaneously, such as MEK pathway inhibition [50,51]. Therefore, the identification of low-frequency KRAS mutations in patients who qualify for anti-EGFR therapy could be an indication for dual first-line therapy with treatment such as MEK inhibitors and is an area worth investigating but is beyond the scope of this study.

5. Conclusions

Our results show a non-significant trend towards an increased risk of disease recurrence, decreased overall survival, and decreased cancer-specific survival for patients with KRAS mutation-positive tissue at the time of diagnosis and initial treatment. These results were not reiterated in ctDNA, which failed to show any significant association between KRAS mutation status and overall survival, cancer-specific survival, or recurrence-free survival.

Supplementary Materials

The following supporting information can be downloaded at https://0-www-mdpi-com.brum.beds.ac.uk/article/10.3390/gastroent15010008/s1, Supplementary Material S1—Methods of plasma separation and genomic DNA extraction.

Author Contributions

Conceptualization, J.P., P.P., and R.J.S.; methodology, J.P., P.P., and R.J.S.; software, J.P.; validation, G.C., P.P., and R.J.S.; formal analysis, J.P.; investigation, J.P.; resources, J.P., P.P. and R.J.S.; data curation, J.P., J.Z., and G.C.; writing—original draft preparation, J.P.; writing—review and editing, G.C., J.Z., P.P., and R.J.S.; supervision, P.P. and R.J.S.; project administration, J.P.; funding acquisition, J.P., P.P., and R.J.S. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the University of Newcastle RHD stipend and the Hunter Medical Research Institute, HMRI Grant 17-63.

Institutional Review Board Statement

This study was conducted in accordance with the Helsinki Declaration and was approved by the Hunter New England Human Research and Ethics Committee (2019/ETH01147, 2019/ETH10205).

Informed Consent Statement

Informed consent was obtained from all subjects involved in this study.

Data Availability Statement

Part of the data presented in this study are available within the article and Supplementary Material S1 file. The rest of the data presented in this study are available on request to the corresponding author. The data are not publicly available due to ethics approval restrictions and privacy reasons for the participants in this study.

Acknowledgments

The authors are grateful to the John Hunter Hospital colorectal surgeons for their help in recruiting patients and collecting samples for this research.

Conflicts of Interest

The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

References

  1. Bray, F.; Ferlay, J.; Soerjomataram, I.; Siegel, R.L.; Torre, L.A.; Jemal, A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA-Cancer J. Clin. 2018, 68, 394–424. [Google Scholar] [CrossRef]
  2. Australian Institute of Health and Welfare. Colorectal and Other Digestive-Tract Cancers; Cancer series no. 114. Cat. no. 117; AIHW: Canberra, Australia, 2018. [Google Scholar]
  3. Andre, T.; Boni, C.; Navarro, M.; Tabernero, J.; Hickish, T.; Topham, C.; Bonetti, A.; Clingan, P.; Bridgewater, J.; Rivera, F.; et al. Improved overall survival with oxaliplatin, fluorouracil and leucovorin as adjuvant treatment in stage II or III colon cancer in the MOSAIC Trial. J. Clin. Oncol. 2009, 27, 3109–3116. [Google Scholar] [CrossRef]
  4. Tie, J.; Cohen, J.; Lahouel, K.; Lo, S.; Wang, Y.; Kosmider, S.; Wong, R.; Shapiro, J.; Lee, M.; Harris, S.; et al. Circulating tumour DNA analysis guiding adjuvant therapy in stage II colon cancer. NEJM 2022, 386, 2261–2272. [Google Scholar] [CrossRef]
  5. Benhattar, J.; Losi, L.; Chaubert, P.; Givel, J.C.; Costa, J. Prognostic significance of K-ras mutations in colorectal carcinoma. Gastroenterology 1993, 104, 1044–1048. [Google Scholar] [CrossRef]
  6. Lee, J.C.; Wang, S.T.; Lai, M.D.; Lin, Y.J.; Yang, H.b. K-ras gene mutation is a useful predictor of the survival of early stage colorectal cancers. Anticancer Res. 1996, 16, 3839–3844. [Google Scholar] [PubMed]
  7. Dinu, D.; Dobre, M.; Panaitescu, E.; Bîrla, R.; Losif, C.; Hoara, P.; Caragui, A.; Boeriu, M.; Constantinoiu, S.; Ardeleanu, C. Prognostic significance of KRAS gene mutations in colorectal cancer–preliminary study. J. Med. Life 2014, 7, 581–587. [Google Scholar]
  8. Spindler, K.; Pallisgaard, N.; Appelt, A.; Andersen, R.; Schou, J.; Nielsen, D.; Pfeiffer, P.; Yilmaz, M.; Johansen, J.; Hoegdall, E.; et al. Clinical utility of KRAS status in circulating plasma DNA compared to archival tumour tissue from patients with metastatic colorectal cancer treated with anti-epidermal growth factor receptor therapy. Eur. J. Cancer 2015, 51, 2678–2685. [Google Scholar] [CrossRef]
  9. Li, Z.; Chen, Y.; Wang, D.; Wang, G.; He, L.; Suo, J. Detection of KRAS mutations and their associations with clinicopathological features and survival in Chinese colorectal cancer patients. J. Int. Med. Res. 2012, 40, 1589–1598. [Google Scholar] [CrossRef] [PubMed]
  10. Tanaka, M.; Omura, K.; Watanabe, Y.; Oda, Y.; Nakanishi, I. Prognostic factors of colorectal-cancer: K-ras mutation, overexpression of the P53-protein, and cell proliferative activity. J. Surg. Oncol. 1994, 57, 57–64. [Google Scholar] [CrossRef] [PubMed]
  11. Dix, B.R.; Robbins, P.; Soong, R.; Jenner, D.; House, A.K.; Iacopetta, B.J. The common molecular genetic alterations in Dukes’ B and C colorectal carcinomas are not short-term prognostic indicators of survival. Int. J. Cancer 1994, 59, 747–751. [Google Scholar] [CrossRef]
  12. Zlobec, I.; Kovac, M.; Erzberger, P.; Molinari, F.; Bihl, M.P.; Rufle, A.; Foerster, A.; Frattini, M.; Terracciano, L.; Heinimann, K.; et al. Combined analysis of specific KRAS mutation, BRAF and microsatellite instability identifies prognostic subgroups of sporadic and hereditary colorectal cancer. Int. J. Cancer 2010, 127, 2569–2575. [Google Scholar] [CrossRef]
  13. Shen, H.; Yuan, Y.; Hu, H.G.; Zhong, X.; Ye, X.X.; Li, M.D.; Feng, W.J.; Zheng, S. Clinical significance of K-ras and BRAF mutations in Chinese colorectal cancer patients. World J. Gastroenterol. 2011, 17, 809–816. [Google Scholar] [CrossRef]
  14. Won, D.D.; Lee, J.I.; Lee, I.K.; Oh, S.T.; Jung, E.S.; Lee, S.H. The prognostic significance of KRAS and BRAF mutation status in Korean colorectal cancer patients. BMC Cancer 2017, 17, 403. [Google Scholar] [CrossRef] [PubMed]
  15. Andreyev, H.J.; Norman, A.R.; Cunningham, D.; Oates, J.R.; Clarke, P.A. Kirsten ras mutations in patients with colorectal cancer: The multicenter “RASCAL” study. J. Natl. Cancer Inst. 1998, 90, 675–684. [Google Scholar] [CrossRef] [PubMed]
  16. Ryan, B.M.; Lefort, F.; McManus, R.; Daly, J.; Keeling, P.W.N.; Weir, D.G.; Kelleher, D. A prospective study of circulating mutant KRAS2 in the serum of patients with colorectal neoplasia: Strong prognostic indicator in postoperative follow up. J. Clin. Pathol.-Mol. Pathol. 2003, 56, 172–179. [Google Scholar] [CrossRef]
  17. Lecomte, T.; Berger, A.; Zinzindohoue, F.; Micard, S.; Landi, B.; Blons, H.; Beaune, P.; Cugnenc, P.H.; Laurent-Puig, P. Detection of free-circulating tumor-associated DNA in plasma of colorectal cancer patients and its association with prognosis. Int. J. Cancer 2002, 100, 542–548. [Google Scholar] [CrossRef] [PubMed]
  18. Bazan, V.; Bruno, L.; Augello, C.; Agnese, V.; Calo, V.; Corsale, S.; Gargano, G.; Terrasi, M.; Schiro, V.; Fede, G.; et al. Molecular detection of TP53, Ki-Ras and p16INK4A promoter methylation in plasma of patients with colorectal cancer and its association with prognosis. Results of a 3-year GOIM (Gruppo Oncologico dell’Italia Meridionale) prospective study. Ann. Oncol. 2006, 17 (Suppl. 7), vii84–vii90. [Google Scholar] [CrossRef] [PubMed]
  19. Lindforss, U.; Zetterquist, H.; Papadogiannakis, N.; Olivecrona, H. Persistence of K-ras mutations in plasma after colorectal tumor resection. Anticancer Res. 2005, 25, 657–661. [Google Scholar] [PubMed]
  20. Wang, J.Y.; Hsieh, J.S.; Chang, M.Y.; Huang, T.J.; Chen, F.M.; Cheng, T.L.; Alexandersen, K.; Huang, Y.S.; Tzou, W.S.; Lin, S.R. Molecular detection of APC, K-ras, and p53 mutations in the serum of colorectal cancer patients as circulating biomarkers. World J. Surg. 2004, 28, 721–726. [Google Scholar] [CrossRef]
  21. Trevisiol, C.; Di Fabio, F.; Nascimbeni, R.; Peloso, L.; Salbe, C.; Ferruzzi, E.; Salerni, B.; Gion, M. Prognostic value of circulating KRAS2 gene mutations in colorectal cancer with distant metastases. Int. J. Biol. Markers 2006, 21, 223–228. [Google Scholar] [CrossRef]
  22. Sefrioui, D.; Sarafan-Vasseur, N.; Beaussire, L.; Baretti, M.; Gangloff, A.; Blanchard, F.; Clatot, F.; Sabourin, J.; Sesboüé, R.; Frebourg, T.; et al. Clinical value of chip-based digital-PCR platform for the detection of circulating DNA in metastatic colorectal cancer. Dig. Liver Dis. 2015, 47, 884–890. [Google Scholar] [CrossRef]
  23. Tie, J.; Kinde, I.; Wang, Y.; Wong, H.L.; Skinner, I.; Wong, R.; Steel, M.; Diaz, L.; Papadopoulos, N.; Kosmider, S.; et al. Circulating tumor DNA (ctDNA) as a marker of recurrence risk in stage II colon cancer (CC). J. Clin. Oncol. 2014, 32 (Suppl. 15), 11015. [Google Scholar] [CrossRef]
  24. Tie, J.; Wang, Y.; Tomasetti, C.; Li, L.; Springer, S.; Kinde, I.; Silliman, N.; Tacey, M.; Wong, H.L.; Christie, M.; et al. Circulating tumor DNA analysis detects minimal residual disease and predicts recurrence in patients with stage II colon cancer. Sci. Transl. Med. 2016, 8, 346ra92. [Google Scholar] [CrossRef]
  25. El Messaoudi, S.; Mouliere, F.; Du Manoir, S.; Bascoul-Mollevi, C.; Gillet, B.; Nouaille, M.; Fiess, C.; Crapez, E.; Bibeau, F.; Theillet, C.; et al. Circulating DNA as a Strong Multimarker Prognostic Tool for Metastatic Colorectal Cancer Patient Management Care. Clin. Cancer Res. Off. J. Am. Assoc. Cancer Res. 2016, 22, 3067–3077. [Google Scholar] [CrossRef]
  26. Spindler, K.G.; Appelt, A.L.; Pallisgaard, N.; Andersen, R.F.; Jakobsen, A. KRAS-mutated plasma DNA as predictor of outcome from irinotecan monotherapy in metastatic colorectal cancer. Br. J. Cancer 2013, 109, 3067–3072. [Google Scholar] [CrossRef]
  27. Bai, Y.Q.; Liu, X.J.; Wang, Y.; Ge, F.J.; Zhao, C.H.; Fu, Y.L.; Lin, L.; Xu, J.M. Correlation analysis between abundance of K-ras mutation in plasma free DNA and its correlation with clinical outcome and prognosis in patients with metastatic colorectal cancer. Zhonghua Zhong Liu Za Zhi 2013, 35, 666–671. [Google Scholar] [PubMed]
  28. Xu, J.M.; Liu, X.J.; Ge, F.J.; Lin, L.; Wang, Y.; Sharma, M.R.; Liu, Z.Y.; Tommasi, S.; Paradiso, A. KRAS mutations in tumour tissue and plasma by different assays predict survival of patients with metastatic colorectal cancer. J. Exp. Clin. Cancer Res. 2014, 33, 104. [Google Scholar] [CrossRef] [PubMed]
  29. Kim, S.T.; Chang, W.J.; Jin, L.; Sung, J.S.; Choi, Y.J.; Kim, Y.H. Can serum be used for analyzing the KRAS mutation status in patients with advanced colorectal cancer? Cancer Res. Treat. 2015, 47, 796–803. [Google Scholar] [CrossRef] [PubMed]
  30. Lin, J.K.; Lin, P.C.; Lin, C.H.; Jiang, J.K.; Yang, S.H.; Liang, W.Y.; Chen, W.S.; Chang, S.C. Clinical relevance of alterations in quantity and quality of plasma DNA in colorectal cancer patients: Based on the mutation spectra detected in primary tumors. Ann. Surg. Oncol. 2014, 21 (Suppl. 4), S680–S686. [Google Scholar] [CrossRef] [PubMed]
  31. Edge, S.; Byrd, D.; Compton, C.; Fritz, A.; Greene, F.; Trotti, A. AJCC Cancer Staging Manual, 7th ed.; Springer: New York, NY, USA, 2010. [Google Scholar]
  32. Armbruster, D.; Pry, T. Limit of blank, limit of detection and limit of quantification. Clin. Biochem. Rev. 2008, 9 (Suppl. 1), S49–S52. [Google Scholar]
  33. Cefali, M.; Epistolio, S.; Palmarocchi, M.; Frattini, M.; De Dosso, S. Research progress on KRAS mutations in colorectal cancer. J. Cancer Metastasis Treat. 2021, 7, 26. [Google Scholar] [CrossRef]
  34. Sefrioui, D.; Beaussire, L.; Clatot, F.; Delacour, J.; Perdrix, A.; Frebourg, T.; Michel, P.; Di Fiore, F.; Sarafan-Vasseur, N. Heparinase enables reliable quantification of circulating tumour DNA from heparinized plasma samples by droplet digital PCR. Clin. Chem. Acta 2017, 472, 75–79. [Google Scholar] [CrossRef] [PubMed]
  35. Dingle, T.; Sedlak, R.; Cook, L.; Jerome, K. Tolerance of droplet-digital PCR versus real-time quantitative PCR to inhibitory substances. Clin. Chem. 2013, 59, 1670–1672. [Google Scholar] [CrossRef] [PubMed]
  36. Kojabad, A.; Farzanehpour, M.; Galeh, H.; Dorostkar, R.; Jafarpour, A.; Bolandian, M.; Nodooshan, M. Droplet digital PCR of viral DNA/RNA, current progress, challenges, and future perspectives. J. Med. Virol. 2021, 93, 4182–4197. [Google Scholar] [CrossRef]
  37. Jones, R.; Sutton, P.; Evans, J.; Clifford, R.; McAvoy, A.; Lewis, J.; Rousseau, A.; Mountford, R.; McWhirter, D.; Malik, H. Specific mutations in KRAS codon 12 are associated with worse overall survival in patients with advanced and recurrent colorectal cancer. BJC 2017, 116, 923–929. [Google Scholar] [CrossRef] [PubMed]
  38. Imamura, Y.; Morikawa, T.; Liao, X.; Lochhead, P.; Kuchiba, A.; Yamauchi, M.; Qian, Z.; Nishihara, R.; Meyerhardt, J.; Haigis, K.; et al. Specific mutations in KRAS codons 12 and 13, and patient prognosis in 1075 BRAF-wild-type colorectal cancers. Clin Cancer Res. 2012, 18, 4753–4763. [Google Scholar] [CrossRef]
  39. Modest, D.; Ricard, I.; Heinemann, V.; Hegewisch-Becker, S.; Schmiegel, W.; Porschen, R.; Stintzing, S.; Graeven, U.; Arnold, D.; Weikersthal, L.; et al. Outcome according to KRAS-, NRAS- and BRAF-mutation as well as KRAS mutation variants: Pooled analysis of five randomized trials in metastatic colorectal cancer by the AIO colorectal cancer study group. Ann. Oncol. 2016, 27, 1746–1753. [Google Scholar] [CrossRef]
  40. Mendoza-Moreno, F.; Diez-Alonso, M.; Matias-Garcia, B.; Ovejero-Merino, E.; Gómez-Sanz, R.; Blázquez-Martin, A.; Quiroga-Valcárcel, A.; Molina, R.; San-Juan, A.; Barrena-Blázquez, S.; et al. Prognostic factors of survival in patients with peritoneal metastasis from colorectal cancer. J. Clin. Med. 2022, 11, 4922. [Google Scholar] [CrossRef]
  41. Alkader, M.; Altaha, R.; Badwan, S.; Halalmeh, A.; Al-Khawaldeh, M.; Atmeh, M.; Jabali, E.; Attieh, O.; Al-Soudi, H.; Alkhatib, L.; et al. Impact of KRAS mutation on survival outcome of patients with metastatic colorectal cancer in Jordan. Cureus 2023, 15, e33736. [Google Scholar] [CrossRef]
  42. Kidess, E.; Heirich, K.; Wiggin, M.; Vysotskaia, V.; Visser, B.; Marziali, A.; Wiedenmann, B.; Norton, J.; Lee, M.; Jeffrey, S.; et al. Mutation profiling of tumour DNA from plasma and tumour tissue of colorectal cancer patients with a novel, high-sensitivity multiplexed mutation detection platform. Oncotarget 2014, 6, 2549–2561. [Google Scholar] [CrossRef]
  43. Pedersen, S.; Symonds, E.; Baker, R.; Murray, D.; McEvoy, A.; Van Doorn, S.; Mundt, M.; Cole, S.; Gopalsamy, G.; Mangira, D.; et al. Evaluation of an assay for methylated BCAT1 and IKZF1 in plasma for detection of colorectal neoplasia. BMC Cancer 2015, 15, 654. [Google Scholar] [CrossRef]
  44. Church, T.; Wandell, M.; Lofton-Day, C.; Mongin, S.; Burger, M.; Payne, S.; Castanos-Vélez, E.; Blumenstein, B.; Rösch, T.; Osborn, N.; et al. Prospective evaluation of methylated SEPT9 in plasma for detection of asymptomatic colorectal cancer. Gut 2014, 63, 317–325. [Google Scholar] [CrossRef]
  45. Diaz, L.; Williams, R.; Wu, J.; Kinde, I.; Hecht, J.R.; Berlin, J.; Allen, B.; Bozic, I.; Reiter, J.; Nowak, M.; et al. The molecular evolution of acquired resistance to targeted EGFR blockade in colorectal cancers. Nature 2012, 486, 537–540. [Google Scholar] [CrossRef]
  46. Misale, S.; Yaeger, R.; Hobor, S.; Scala, E.; Janakiraman, M.; Liska, D.; Valtorta, E.; Schiavo, R.; Buscarino, M.; Siravegna, G.; et al. Emergence of KRAS mutations and acquired resistance to anti-EGFR therapy in colorectal cancer. Nature 2012, 486, 532–536. [Google Scholar] [CrossRef]
  47. Van Emburgh, B.; Arena, S.; Siravegna, G.; Lazzari, L.; Crisafulli, G.; Corti, G.; Mussolin, B.; Baldi, F.; Buscarino, M.; Bartolini, A.; et al. Acquired RAS or EGFR mutations and duration of response to EGFR blockade in colorectal cancer. Nat. Commun. 2016, 7, 13665. [Google Scholar] [CrossRef]
  48. Tougeron, D.; Lecomte, T.; Pagès, J.; Villalve, C.; Collin, C.; Ferru, A.; Tourani, J.; Silvain, C.; Levillain, P.; Karayan-Tapon, L. Effect of low-frequency KRAS mutations on the response to anti-EGFR therapy in metastatic colorectal cancer. Ann. Oncol. 2013, 24, 1267–1273. [Google Scholar] [CrossRef]
  49. Karapetis, C.; Khambata-Ford, S.; Jonker, D.; O’Callaghan, C.; Tu, D.; Tebbutt, N.; Simes, R.; Chalchal, H.; Shapiro, J.; Robitaille, S.; et al. K-ras mutations and benefit from Cetuximab in advanced colorectal cancer. NEJM 2008, 359, 1757–1765. [Google Scholar] [CrossRef]
  50. Troiani, T.; Napolitano, S.; Vitagliano, D.; Morgillo, F.; Capasso, A.; Sforza, V.; Nappi, A.; Ciardiello, D.; Ciardiello, F.; Martinelli, E. Primary and acquired resistance of colorectal cancer cells to anti-EGFR antibodies converge on MEK/ERK pathway activation and can be overcome by combined MEK/EGFR inhibition. Clin. Cancer Res. 2014, 20, 3775–3786. [Google Scholar] [CrossRef]
  51. Zhou, J.; Ji, Q.; Li, Q. Resistance to anti-EGFR therapies in metastatic colorectal cancer: Underlying mechanisms and reversal strategies. J. Exp. Clin. Cancer Res. 2021, 40, 328. [Google Scholar] [CrossRef]
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Figure 1. Kaplan–Meier curve of OS vs. ctDNA KRAS status.
Figure 1. Kaplan–Meier curve of OS vs. ctDNA KRAS status.
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Figure 2. Kaplan–Meier curve of CSS vs. ctDNA KRAS status.
Figure 2. Kaplan–Meier curve of CSS vs. ctDNA KRAS status.
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Figure 3. Kaplan–Meier curve of RFS vs. ctDNA KRAS status.
Figure 3. Kaplan–Meier curve of RFS vs. ctDNA KRAS status.
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Figure 4. Kaplan–Meier curve of OS vs. tissue DNA KRAS status.
Figure 4. Kaplan–Meier curve of OS vs. tissue DNA KRAS status.
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Figure 5. Kaplan–Meier curve of CSS vs. tissue DNA KRAS status.
Figure 5. Kaplan–Meier curve of CSS vs. tissue DNA KRAS status.
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Figure 6. Kaplan–Meier curve of RFS vs. tissue DNA KRAS status.
Figure 6. Kaplan–Meier curve of RFS vs. tissue DNA KRAS status.
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Table 1. Demographic and clinical characteristics of patients by ctDNA KRAS status.
Table 1. Demographic and clinical characteristics of patients by ctDNA KRAS status.
NegativePositiveTotalp-Value
Exact
CharacteristicResponse/Statistic(n = 57)(n = 23)(N = 80)
SexMale33 (58%)9 (39%)42 (53%)0.146
Female24 (42%)14 (61%)38 (48%)
Smoking statusNon-smoker30 (53%)18 (78%)48 (60%)0.038
Ex-smoker20 (35%)2 (9%)22 (28%)
Smoker7 (12%)3 (13%)10 (13%)
Age at operationmean (SD)68.91 (13.52)72.87 (9.28)70.04 (12.52)0.374
median71.6073.6072.23
BMImean (SD)28.99 (5.81)28.56 (4.80)28.87 (5.52)0.642
median27.9628.1028.10
CCI scoremean (SD)4.91 (1.56)4.84 (2.31)4.86 (2.11)0.429
median555
RecurrenceNo42 (74%)15 (65%)57 (71%)0.586
Yes15 (26%)8 (35%)23 (29%)
Site of cancerRight20 (35%)9 (39%)29 (36%)1.0
Left34 (60%)14 (61%)48 (60%)
Synchronous2 (3%02 (3%)
Missing1 (2%)01 (1%)
Tumour gradeWell or mod45 (80%)15 (65%)60 (76%)0.249
Poorly4 (7.1%)5 (22%)9 (11%)
Mucinous or medullary7 (13%)3 (13%)10 (13%)
Missing101
Pathological stageIn situ3 (5.3%)03 (3.8%)0.853
Stage 113 (23%)5 (22%)18 (23%)
Stage 220 (35%)7 (30%)27 (34%)
Stage 317 (30%)9 (39%)26 (33%)
Stage 44 (7.0%)2 (8.7%)6 (7.5%)
Resection marginR052 (91%)20 (87%)72 (90%)0.830
R12 (4%)1 (4%)3 (4%)
R23 (5%)2 (9%)5 (6%)
Adjuvant chemotherapyReceived18 (32%)13 (57%)31 (39%)0.046
Not received39 (68%)10 (43%)49 (61%)
Table 2. OS, CSS, and RFS by ctDNA KRAS status.
Table 2. OS, CSS, and RFS by ctDNA KRAS status.
NegativePositiveTotalLog-Rank
CharacteristicResponse/Statistic(n = 57)(n = 23)(N = 80)p-Value
Overall survival *mean (SD)6.42 (0.357)6.30 (0.375)6.44 (0.295)0.891
Overall survival **mean (SD)4.34 (0.206)4.67 (0.175)4.43 (0.155)0.832
Survival outcomeCensored37 (65%)15 (65%)52 (65%)
Death16 (28%)6 (26%)22 (27%)
Missing data/Excluded4 (7%)2 (9%)6 (8%)
Cancer-specific survival *mean (SD)6.87 (0.332)6.44 (0.365)6.81 (0.282)0.690
Cancer-specific survival **mean (SD)4.49 (0.197)4.72 (0.175)4.55 (0.146)0.747
Survival statusCensored43 (75%)16 (70%)53 (66%)
Cancer-specific death10 (18%)5 (22%)21 (26%)
Missing data4 (7%)2 (9%)6 (8%)
Recurrence-free survival *mean (SD)6.37 (0.376)5.12 (0.548)6.22 (0.331)0.590
Recurrence-free survival **mean (SD)4.26 (0.205)3.89 (0.367)4.15 (0.182)0.616
Survival statusCensored37 (65%)14 (61%)51 (64%)
Recurrence14 (25%)7 (30%)21 (26%)
Missing data6 (11%)2 (9%)8 (10%)
* unadjusted follow-up survival data, ** survival adjusted to 5-year endpoints
Table 3. OS, CSS, and RFS hazard ratio by ctDNA KRAS status.
Table 3. OS, CSS, and RFS hazard ratio by ctDNA KRAS status.
Crude Adjusted +
ModelHR (95% CI)p-ValueNHR (95% CI)p-ValueN
Overall survival *0.94 (0.37, 2.40)0.891740.97 (0.38, 2.50)0.95274
Cancer survival *1.24 (0.42, 3.65)0.691741.26 (0.43, 3.71)0.67374
Recurrence *1.28 (0.52, 3.18)0.591721.28 (0.52, 3.17)0.59872
Overall survival **0.90 (0.35, 2.31)0.833740.94 (0.37, 2.40)0.88874
Cancer survival **1.19 (0.41, 3.49)0.748741.21 (0.41, 3.54)0.73174
Recurrence **1.26 (0.51, 3.12)0.617721.26 (0.51, 3.11)0.62372
+ Adjustment for CCI only displayed in this example, * unadjusted follow-up survival data, ** survival adjusted to 5-year endpoints.
Table 4. Demographic and clinical characteristics of patients by tissue DNA KRAS status.
Table 4. Demographic and clinical characteristics of patients by tissue DNA KRAS status.
NegativePositiveTotalp-Value
Exact
CharacteristicResponse/Statistic(n = 29)(n = 78)(N = 107)
SexMale15 (52%)45 (58%)60 (56%)0.663
Female14 (48%)33 (42%)47 (44%)
Smoking statusNon-smoker19 (66%)48 (62%)67 (63%)0.286
Ex-smoker5 (17%)23 (29%)28 (26%)
Smoker5 (17%)7 (9%)12 (11%)
Age at operationmean (SD)65.66 (14.18)71.32 (11.38)69.78 (12.39)0.623
median65.6073.5571.60
BMImean (SD)27.96 (6.22)28.49 (4.91)28.34 (5.28)0.346
median28.8827.3027.75
CCI scoremean (SD)4.48 (1.7)5.42 (1.96)5.17 (1.93)0.531
median455
RecurrenceNo24 (83%)59 (76%)83 (78%)0.603
Yes5 (17%)19 (24%)24 (22%)
Site of cancerRight8 (28%)32 (41%)40 (37%)0.444
Left21 (72%)43 (55%)64 60%)
Synchronous02 (3%)2 (2%)
Missing01 (1%)1 (1%)
Tumour gradeWell or mod23 (79%)59 (77%)82 (77%)1.0
Poorly2 (6.9%)7 (9.1%)9 (8.5%)
Mucinous or medullary4 (14%)11 (14%)15 (14%)
Missing011
Pathological stageIn situ3 (10%)2 (2.6%)5 (4.7%)0.413
Stage 16 (21%)19 (24%)25 (23%)
Stage 27 (24%)26 (33%)33 (31%)
Stage 312 (41%)26 (33%)38 (36%)
Stage 41 (3.4%)5 (6.4%)6 (5.6%)
Resection marginR028 (97%)73 (94%101 (94%)0.829
R102 (3%)2 (2%)
R21 (3%)3(4%)4 (4%)
Adjuvant chemotherapyReceived14 (48%)27 (35%)41 (38%)0.263
Not received15 (52%)51 (65%)66 (62%)
Table 5. OS, CSS, and RFS by tissue DNA KRAS status.
Table 5. OS, CSS, and RFS by tissue DNA KRAS status.
NegativePositiveTotalLog-Rank
CharacteristicResponse/Statistic(n = 29)(n = 78)(N = 107)p-Value
Overall survival time *mean (SD)6.45 (0.352)6.22 (0.321)6.42 (0.264)0.251
Overall survival time **mean (SD)4.61 (0.218)4.23 (0.179)4.34 (0.142)0.193
Survival outcomeCensored22 (76%)48 (62%)70 (65%)
Death6 (21%)26 (33%)32 (30%)
Missing data/excluded1 (3%)4 (5%)5 (5%)
Cancer-specific survival time *mean (SD)6.71 (0.319)6.73 (0.303)6.87 (0.248)0.411
Cancer-specific survival time **mean (SD)4.71 (0.215)4.41 (0.168)4.50 (0.132)0.312
Survival statusCensored24 (83%)57 (73%)81 (76%)
Cancer-specific death4 (14%)17 (22%)21 (20%)
Missing data/excluded1 (3%)4 (5%)5 (5%)
Recurrence-free survival time *mean (SD)6.27 (0.418)6.46 (0.345)6.59 (0.284)0.439
Recurrence-free survival time **mean (SD)4.41 (0.251)4.16 (0.196)4.23 (0.158)0.443
Survival statusCensored22 (76%)54 (69%)76 (71%)
Recurrence5 (17%)18 (23%)23 (22%)
Missing data/excluded2 (7%)6 (8%)8 (7%)
* Unadjusted follow-up survival data, ** survival adjusted to 5-year endpoints.
Table 6. OS, CSS, and RFS by tissue DNA KRAS > 10MAF status.
Table 6. OS, CSS, and RFS by tissue DNA KRAS > 10MAF status.
NegativePositiveTotalLog-Rank
CharacteristicResponse/Statistic(n = 84)(n = 23)(N = 107)p-Value
Overall survival time *mean (SD)6.46 (0.280)6.12 (0.607)6.12 (0.264)0.628
Overall survival time **mean (SD)4.39 (0.154)4.14 (0.365)4.34 (0.142)0.544
Survival outcomeCensored56 (67%)14 (61%)70 (65%)
Death24 (29%)8 (35%)32 (30%)
Missing data/excluded4 (5%)1 (4%)5 (5%)
Cancer-specific survival time *mean (SD)6.95 (0.251)6.43 (0.606)6.87 (0.248)0.476
Cancer-specific survival time **mean (SD)4.58 (0.138)4.19 (0.376)4.50 (0.132)0.382
Survival statusCensored65 (77%)16 (70%)81 (76%)
Cancer-specific death15 (18%)6 (26%)21 (20%)
Missing data/excluded4 (5%)1 (4%)5 (5%)
Recurrence-free survival time *mean (SD)6.54 (0.306)6.43 (0.660)6.59 (0.284)0.914
Recurrence-free survival time **mean (SD)4.28 (0.173)4.06 (0.373)4.23 (0.158)0.924
Survival statusCensored60 (71%)16 (70%)76 (71%)
Recurrence18 (21%)5 (22%)23 (22%)
Missing data/excluded6 (7%)2 (9%)8 (7%)
* Unadjusted follow-up survival data, ** survival adjusted to 5-year endpoints.
Table 7. OS, CSS, and RFS hazard ratio by tissue DNA KRAS status.
Table 7. OS, CSS, and RFS hazard ratio by tissue DNA KRAS status.
CrudeAdjusted +
ModelCut-Off MethodHR (95% CI)p-ValueNHR (95% CI)p-ValueN
OS *LoD1.68 (0.69, 4.09)0.2571021.48 (0.60, 3.67)0.395102
CSS *1.58 (0.53, 4.73)0.4151021.49 (0.49, 4.53)0.485102
RFS *1.48 (0.55, 3.98)0.441991.43 (0.52, 3.91)0.48899
OS *>10% MAF1.22 (0.55, 2.73)0.6281021.04 (0.45, 2.36)0.934102
CSS *1.42 (0.54, 3.69)0.4751021.32 (0.49, 3.51)0.581102
RFS *1.06 (0.39, 2.84)0.914990.99 (0.36, 2.75)0.99099
OS *>5% MAF1.41 (0.65, 3.06)0.3871021.21 (0.55, 2.67)0.643102
CSS *1.74 (0.70, 4.35)0.2371021.64 (0.64, 4.19)0.305102
RFS *1.34 (0.53, 3.39)0.540991.28 (0.49, 3.34)0.61799
OS *>1% MAF1.25 (0.57, 2.71)0.5761021.10 (0.50, 2.41)0.820102
CSS *1.55 (0.62, 3.87)0.3491021.46 (0.58, 3.71)0.422102
RFS *1.17 (0.46, 2.97)0.743991.12 (0.43, 2.89)0.81799
OS **LoD1.78 (0.73, 4.33)0.2021021.56 (0.63, 3.84)0.336102
CSS **1.74 (0.58, 5.16)0.3201021.61 (0.53, 4.86)0.399102
RFS **1.47 (0.55, 3.96)0.446991.42 (0.52, 3.90)0.49199
OS **>10% MAF1.28 (0.57, 2.85)0.5481021.05 (0.46, 2.40)0.900102
CSS **1.52 (0.59, 3.92)0.3871021.37 (0.52, 3.64)0.526102
RFS **1.05 (0.39, 2.83)0.924990.99 (0.36, 2.74)0.98399
OS **>5% MAF1.47 (0.68, 3.18)0.3261021.23 (0.56, 2.72)0.612102
CSS **1.86 (0.75, 4.60)0.1821021.70 (0.67, 4.34)0.268102
RFS **1.33 (0.52, 3.73)0.548991.27 (0.49, 3.32)0.62499
OS **>1% MAF1.29 (0.60, 2.78)0.5221021.11 (0.50, 2.43)0.802102
CSS **1.63 (0.66, 4.03)0.2941021.50 (0.59, 3.79)0.390102
RFS **1.16 (0.46, 2.94)0.757991.11 (0.43, 2.87)0.82999
+ Adjustment for CCI only displayed in this example, * unadjusted follow-up survival data, ** survival adjusted to 5-year endpoints, MAF = mutation allele frequency, LOD = limit of detection.
Table 8. Concordance between ctDNA and tissue KRAS status.
Table 8. Concordance between ctDNA and tissue KRAS status.
ctDNA
PositiveNegativeTotal
Tumour tissue
(LoD Cut-off)		Positive153449
Negative31417
Total184866
Tumour tissue
(10% MAF Cut-off)		Positive6612
Negative124254
Total184866
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Petit, J.; Carroll, G.; Zhao, J.; Pockney, P.; Scott, R.J. The Prognostic Utility of KRAS Mutations in Tissue and Circulating Tumour DNA in Colorectal Cancer Patients. Gastroenterol. Insights 2024, 15, 107-121. https://0-doi-org.brum.beds.ac.uk/10.3390/gastroent15010008

AMA Style

Petit J, Carroll G, Zhao J, Pockney P, Scott RJ. The Prognostic Utility of KRAS Mutations in Tissue and Circulating Tumour DNA in Colorectal Cancer Patients. Gastroenterology Insights. 2024; 15(1):107-121. https://0-doi-org.brum.beds.ac.uk/10.3390/gastroent15010008

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Petit, Joel, Georgia Carroll, Jie Zhao, Peter Pockney, and Rodney J. Scott. 2024. "The Prognostic Utility of KRAS Mutations in Tissue and Circulating Tumour DNA in Colorectal Cancer Patients" Gastroenterology Insights 15, no. 1: 107-121. https://0-doi-org.brum.beds.ac.uk/10.3390/gastroent15010008

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