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Review

Cholera Toxin: An Intracellular Journey into the Cytosol by Way of the Endoplasmic Reticulum

1
GI Cell Biology, Children's Hospital (and Harvard Medical School), 300 Longwood Avenues, Enders 720, Boston, MA 02115, USA
2
The Harvard Digestive Diseases Center, Boston, MA 02115, USA
*
Author to whom correspondence should be addressed.
Received: 27 January 2010 / Revised: 27 February 2010 / Accepted: 2 March 2010 / Published: 5 March 2010
(This article belongs to the Special Issue Enterotoxins)
Cholera toxin (CT), an AB5-subunit toxin, enters host cells by binding the ganglioside GM1 at the plasma membrane (PM) and travels retrograde through the trans-Golgi Network into the endoplasmic reticulum (ER). In the ER, a portion of CT, the enzymatic A1-chain, is unfolded by protein disulfide isomerase and retro-translocated to the cytosol by hijacking components of the ER associated degradation pathway for misfolded proteins. After crossing the ER membrane, the A1-chain refolds in the cytosol and escapes rapid degradation by the proteasome to induce disease by ADP-ribosylating the large G-protein Gs and activating adenylyl cyclase. Here, we review the mechanisms of toxin trafficking by GM1 and retro-translocation of the A1-chain to the cytosol. View Full-Text
Keywords: cholera toxin; retro-translocation; ERAD; retrograde pathway; lipid rafts cholera toxin; retro-translocation; ERAD; retrograde pathway; lipid rafts
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MDPI and ACS Style

Wernick, N.L.B.; Chinnapen, D.J.-F.; Cho, J.A.; Lencer, W.I. Cholera Toxin: An Intracellular Journey into the Cytosol by Way of the Endoplasmic Reticulum. Toxins 2010, 2, 310-325. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins2030310

AMA Style

Wernick NLB, Chinnapen DJ-F, Cho JA, Lencer WI. Cholera Toxin: An Intracellular Journey into the Cytosol by Way of the Endoplasmic Reticulum. Toxins. 2010; 2(3):310-325. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins2030310

Chicago/Turabian Style

Wernick, Naomi L. B., Daniel J.-F. Chinnapen, Jin Ah Cho, and Wayne I. Lencer 2010. "Cholera Toxin: An Intracellular Journey into the Cytosol by Way of the Endoplasmic Reticulum" Toxins 2, no. 3: 310-325. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins2030310

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