Modular 3D In Vitro Artery-Mimicking Multichannel System for Recapitulating Vascular Stenosis and Inflammation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Fabrication of a Modular Device for In Vitro Artery Mimicking
2.3. HASMC–HUVEC Coculture in a Modular Device
2.4. Characterization of the Microfluidic CGG Module
2.5. TNF-α Treatment for the Inflammation Model
2.6. Monocytic THP-1 Cell Adhesion in the Inflammation Model
2.7. Immunofluorescence Staining and Imaging
2.8. Statistical Analysis
3. Results and Discussion
3.1. Fabrication of a Modular 3D In Vitro Artery-Mimicking Multichannel System
3.2. Concentration Gradient Generation in a Multichannel with a Microfluidic CGG Module
3.3. TNF-α Dose-Dependent Adhesion Molecule and vWF Expression in an Inflammation Model
3.4. TNF-α Dose-Dependent Monocyte Adhesion in the Inflammation Model
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Cho, M.; Park, J.-K. Modular 3D In Vitro Artery-Mimicking Multichannel System for Recapitulating Vascular Stenosis and Inflammation. Micromachines 2021, 12, 1528. https://0-doi-org.brum.beds.ac.uk/10.3390/mi12121528
Cho M, Park J-K. Modular 3D In Vitro Artery-Mimicking Multichannel System for Recapitulating Vascular Stenosis and Inflammation. Micromachines. 2021; 12(12):1528. https://0-doi-org.brum.beds.ac.uk/10.3390/mi12121528
Chicago/Turabian StyleCho, Minkyung, and Je-Kyun Park. 2021. "Modular 3D In Vitro Artery-Mimicking Multichannel System for Recapitulating Vascular Stenosis and Inflammation" Micromachines 12, no. 12: 1528. https://0-doi-org.brum.beds.ac.uk/10.3390/mi12121528