1. Introduction
Colorectal carcinoma is the most common tumor affecting the entire digestive tract [
1]. Colorectal cancer is a highly prevalent disease which, according to Globocan 2018, ranks fourth in terms of both incidence (9.7%) and mortality (8.4%) worldwide, for both genders combined [
2,
3,
4], with more than half of patients identified in regions which present a higher degree of development. This malignancy is the second most common type of cancer to affect women (with both incidence and mortality values around 9%), right after breast and cervical cancer, and the third in men, after lung and prostate cancers (with an incidence of 10.1% and a mortality of 8%). Diagnosed in its early stages, colorectal cancer can be treated with considerable success rates [
5], and this is one of the reasons why it is of utmost importance to drastically change the clinical approach to this malignancy, in terms of identifying fast, accurate, minimally invasive diagnostic and prognostic methods, as well as developing more efficient targeted therapies [
4,
6,
7,
8,
9]. Therefore, there is a growing necessity for identifying and characterizing the molecular pathogenesis of colorectal carcinoma [
8,
10,
11].
It has been shown that miRNAs have an important role in tumorigenesis, and the involvement of these transcripts in the etiology of cancer is a subject of study for researchers across the world [
12,
13,
14,
15]. miRNAs are non-coding RNA structures, of 19–24 nucleotides, which are never translated into proteins, but that have a role in the control of the expression of protein coding genes by either physically degrading the messenger RNA or by blocking transcription [
13,
16,
17,
18]. Studies have shown that miRNAs are commonly found in circulation, both in serum and plasma, either free or “encapsulated” within small vesicles named exosomes [
15,
19,
20,
21]. These short transcripts are presumably excreted by tumors and released into circulation; therefore, they carry information regarding the place of origin [
22,
23] and thus are able to play diagnostic and prognostic roles regarding the development and progression of tumors [
13,
14,
21,
24,
25].
High throughput technologies such as microarray or next generation sequencing have allowed the evaluation of molecular profiles in various types of cancers, in an attempt to understand the complexity and heterogeneity of the malignant disease [
15,
21,
26,
27]. Considering all these, one of the main objectives of our study was to evaluate plasma miRNAs and to compare them with the expression levels found in tissue (using The Cancer Genome Atlas (TCGA) data) to identify colorectal-cancer-specific miRNAs. Understanding the regulation of miRNA expression has the potential to shed light on the screening of cancer biomarkers, particularly those related to the response to therapy, and the initiation of resistance behavior.
2. Material and Methods
2.1. TCGA Data, Human Subjects and Clinical Data
The Cancer Genome Atlas (TCGA) is a public database developed to create a comprehensive cancer genomic profile with important implications in biomarker discovery [
28]. Starting from this, we designed and performed a microarray experiment for microRNA evaluation on plasma samples from colorectal cancer (CRC). In order to calculate the differential expression, colorectal samples were compared with plasma collected from healthy controls deposited in our biobank. All samples were obtained by following protocols in strict compliance with national and European regulations regarding the manipulation of biological samples and personal data: all cases were anonymized, all donors were briefed about the study and each gave informed consent. At the same time, the study and all its aspects were approved by the Ethical Committee of The Oncology Institute “Prof. Dr. Ion Chiricuta” (approval number 6346/02.07.2014), which certifies the compliance with the aforementioned regulations, including the proper signing of the informed consent forms by all participants in the study.
TCGA clinical data for the colorectal samples used in present study are presented in
Table 1. The microarray experiment was conducted on a total of 55 CRC plasma samples: 38 samples without chemotherapy (CT) and 17 post-chemotherapy, together with 16 control samples. According to the anatomical site, 25 cancer cases were located on the colon, 25 on the rectum, and five cases had colorectal localization. The complete demographic characteristics of the patients are presented in
Table 2, alongside some information regarding the healthy controls for the microarray study.
Table 3 contains demographic characteristics for the validation set.
2.2. Plasma Preparation and RNA Isolation for Colorectal Cancer Patients and Healthy Controls
At the time of sample collection, peripheral blood on EDTA (Ethylenediaminetetraacetic acid) was obtained from each patient after the initial diagnosis, and immediately processed (within two hours from collection) to obtain the plasma by centrifugation at 4000 rpm for 10 minutes at room temperature, under standardized conditions. Plasma was divided into aliquots and deposited at −80 °C. The extraction of miRNAs from the plasma samples was performed using the Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format). After extraction, the nucleic acids were subjected to quantitative and qualitative evaluation, using the NanoDrop instrument, and the Agilent Bioanalyzer (Agilent Technologies, Santa Clara USA), respectively. This way, we were able to ensure the necessary values for the nucleic acid concentrations that are mandatory for the subsequent experimental steps.
2.3. miRNA Microarray Evaluation
The protocol for the miRNA microarray experiment started with achieving a concentration of 100 ng of total RNA for each of the studied samples. Sample hybridization was performed using the Agilent microRNA Spike-In kit, while labeling was done with the miRNA Complete Labeling and Hyb Kit. In order to avoid the possible presence of artifacts, we preceded with a purification step, with the help of Micro Bio-Spin 6 (Biorad, Mississauga, ON, Canada) spin columns, followed by a desiccation step in a vacuum centrifuge, and the resuspension of the pellet in 18 μL of RNase free, microbiologically pure water. The hybridization phase was conducted according to the manufacturer’s recommendations, and the slides (Agilent SurePrint Human miRNA v21.0 microarray, G4872A) were left in the hybridization oven for 20 hours, at a temperature of 55 °C. After washing, the slides were scanned using an Agilent Microarray Scanner.
2.4. Analysis of Microarray Data
After scanning the microarray slides, the Agilent Feature Extraction software (Agilent Technologies) was used to analyze the images and convert them to numeric expression values. The individual files were fed into the Agilent GeneSpring GX program (Agilent Technologies) for data normalization, which was conducted using the quantile algorithm. No baseline transformation was performed. Entities were initially filtered based on their flag values, keeping them acceptable on the “detected” ones. Differentially expressed genes were selected using the “Filter on Volcano Plot” analysis and moderated t-test, with a fold change of 1.5 and a p-value <0.05 for the following comparisons: treated samples versus untreated samples, untreated colon samples versus controls, untreated rectum samples versus controls, and treated rectum samples versus untreated rectum samples. The missing logical comparison “treated colon samples versus untreated colon samples” is due to the fact that the cohort of patients did not include any patients with colon localization of tumors who previously underwent chemotherapy. Whenever possible, the Benjamini–Hochberg (false discovery rate (FDR)) algorithm was applied, to control for false positives.
2.5. miRNA RT-PCR
For testing the candidate miRNAs (miR-1228-3p, miR-4741, miR-642b-3p, miR-195-5p) observed to be differentially expressed in a statistically significant manner in the microarray experiment, RT-PCR was performed starting from 50 ng total RNA. The total RNA was reverse transcribed using a TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystem, 4366596). RT-PCR reactions were performed on the ViiA7 instrument (Applied Biosystems, Foster City, CA, USA) in a 10μL reaction volume containing TaqMan™ Fast Advanced Master Mix (Catalog number: 4444556, Thermo Scientific, Waltham, MA, USA). The expression level was calculated using the 2
−ΔΔCt method, using U6 for normalization and
p < 0.05 was considered statistically significant. A ROC (receiver operating characteristic) analysis was employed to calculate sensitivity and specificity of each biomarker using GraphPad Prism (
https://www.graphpad.com/, Version 6).
2.6. Integrated Analysis of the Altered Plasma miRNAs in Colorectal Cancer
The integrated analysis for the altered miRNA transcripts was performed using Ingenuity Pathway Analysis (IPA; Qiagen, Inc., Valencia, CA, USA), which is a valuable bioinformatics tool used for the identification of the most relevant altered pathways and networks based on the complex database with 302 metabolic networks and 360 signaling pathways. The altered miRNAs are classified based on a significance score by overlapping with these networks and pathways. For the integration of the altered plasma miRNA patterns in drug resistance mechanisms, the NCBI Gene database was used (
www.ncbi.nlm.nih.gov) by searching for the string “miRNA” and “drug resistance” and by selecting the non-coding genes for
Homo sapiens. For evaluating the target genes we used miRTargetLink database (
https://ccb-web.cs.uni-saarland.de/mirtargetlink/).
4. Discussion
Tumor-specific alterations of plasma miRNAs in cancer patients are promising non-invasive biomarkers with diagnostic/prognostic value. Plasma miRNAs have a high stability, therefore emphasizing their promising application as biomarkers [
29,
30]. Moreover, a better comprehension of miRNA trafficking (intracellular, extracellular realized in different forms, free or via vesicles) is essential for understanding the biology of cancer, and a particular attention should be directed to the vesicles that support the processes of exiting and entering the bloodstream circulation. miRNAs are key players known to participate in tumorigenesis and regulate the development and progression of human malignancies [
13,
14]. In our case, we identified differences among plasma miRNA profiles, most of them being downregulated.
A limited number of studies present miRNA profiling data for colorectal cancer at the plasma level. This is not always correlated with what we observed by the overlapping of plasma miRNA data with those from TCGA. Furthermore, we noticed a larger variation in miRNA expression levels among colon and rectal cancer plasma samples, suggesting that colon and rectal cancer should be studied independently, taking into account the molecular and clinical incongruities. Consequently, the general observation is that miRNAs are downregulated in colon and rectal cancer, with similar observations presented in a recent miRNA profiling study on rectal cancer tissue versus normal tissue [
31].
Plasma-released miRNAs may be considered as a promising strategy for increased therapeutic efficacy and particularly for avoiding the activation of drug resistance mechanisms, as we observed in the case of the functional study performed on miR-205 [
32]. The network presented in
Figure 6A is centered on the key gene TP53, which is involved in the regulation of cell proliferation, but was also proved to interfere with drug resistance related pathways [
33]. The modulation of miRNA-processing enzymes Ago2 by miRNAs (
Figure 6B) may be important for the regulation of cell proliferation and EMT (epithelial to mesenchymal transition) based on the fact that they are interconnected with two main effectors of this mechanism (Zeb1 and Zeb2), which are regulated by miR-205. Ago2 and related enzymes involved in miRNA biogenesis are important effectors in colorectal carcinomas [
34]. Ago1 and Ago2 are associated with selective autophagy [
35], and are able to target cell cycle regulators, cell proliferation and apoptosis [
36,
37,
38], as observed in
Figure 6C.
There is growing concern for establishing correlations between miRNA expression in tumors or the alteration of expression levels as a response to chemotherapy and radiosensitivity, which are considered as predictors or modifiers of the response to therapy [
39]. As presented previously, miRNAs can be used as biomarkers, and here we present a specific panel of transcripts (
Figure 7) that can be considered as candidate markers for predicting the response to chemotherapy [
30].
miR-1228-3p was found to be downregulated in our colorectal cancer tissue samples and TCGA dataset. Moreover, microarray and RT-PCR validation assays on plasma samples from the same patients revealed a significant overexpressed profile, a fact that could classify this miRNA as a strong prediction biomarker. The opposite expression level of miR-1228 between plasma and tissue samples is an intriguing outcome, where a possible mechanism could consist of the expulsion of this miRNA in circulation via exosomes for the further rescue of tumor cells from a tumor suppressor miRNA [
40]. The two validated targets of miR-1228 identified using the miRTargetLink database might have important relevance in colorectal cancer (
Figure 4E). The first gene, MOAP1, has been previously identified as an important tumor suppressor molecule linked to the RASSF1A protein, with implications in apoptosis [
41]. Data from The Human Protein Atlas [
42] reveal that this protein is actually downregulated in colorectal cancer patients; the fact is that, in some instances, this can be contradictable with miR-1228-3p expression (which is also downregulated in tumor tissue). However, a possible explanation could involve the methylation status, where the downregulation of MOAP1 is independent of miR-1228 regulation in colorectal cancer due to epigenetic modifications in RASSF1A [
43]. We further concentrated on the second strong validated target gene of miR-1228-3p, CSNK2A2. This gene was previously found as overexpressed in colorectal cancer and was also affiliated with late stages and chemotherapeutic response (5-FU in vitro-related, HCT116 parental and chemotherapy-resistant cell line models using a disease-specific microarray) [
44]. Specifically, CSNK2A2 protects colon cancer cells from TRAIL-induced apoptosis [
45]. A possible explanation for this overexpression pattern in colorectal cancer could be offered by miR-1228 downregulation in tissue samples, where experimental upregulation of the miRNA could stand as a potent therapeutic strategy. With this in mind, miR-1228-3p could become an important biomarker in colorectal cancer and also a therapeutic target due to the paradoxical expression in tissue and plasma samples that most probably involves an exosomal shifting mechanism, and also due to the inhibitory roles of key genes involved in cell death pathways (with further consequences on drug resistance).
Within this framework, the research presented in this manuscript is intended to be further developed in the future, with the purpose of addressing one of its limitations, i.e., the relatively small number of patients enrolled in the study. Thus, we plan to conduct more types of experimental evaluations on larger cohorts of patients, in order to validate and strengthen the present results.
5. Conclusions
Circulating miRNAs represent an important source of biomarkers for minimally invasive diagnosis and prognosis methods. More importantly, the evaluation of treatment response through circulating miRNAs becomes a competitive advantage in cancer treatment and management, where the current methods of evaluation are invasive and restricted in terms of specificity. More efforts in investigation methods will help explain the mechanisms responsible for miRNA-released biofluids, with direct effects upon their exploitation as biomarkers, using standardized methods. This is the case of miR-1224, which is the most upregulated transcript in colorectal plasma samples and which can function as a prediction tool within the oncological field. Moreover, this miRNA was found to have opposite expression levels in tumor samples from the same patients, demonstrating a complex mechanism of regulation, possibly mediated by exosomal shifting between the tumor mass and fluid microenvironment.
Even if considerable progress was achieved in the comprehension of miRNA involvement in colorectal cancer, many queries must still be answered before transcribing miRNA profiling into clinical practice. One of the greatest questions for future years will be related to the identification of specific miRNA signatures for discrimination between different specific subtypes, which need to be highly reproducible and independently predictive of clinical–biological features of the tumor to ameliorate diagnosis and treatment. In the future, clinicians may perhaps be guided by simple tests evaluating the miRNA expression pattern in their patients, making them forcefully valid for cancer personalized medicine.