Increased Uptake of Silica Nanoparticles in Inflamed Macrophages but Not upon Co-Exposure to Micron-Sized Particles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemical and Reagents
2.2. Synthesis and Characterization of Particles
2.3. Cell Culture
2.4. Cell Seeding
2.5. Exposures to Silica Particles
2.5.1. Pretreatment of Cells with LPS
2.5.2. Sequential Particle Exposure
2.5.3. Simultaneous Particle Exposure
2.5.4. Controls
2.6. Confocal Laser Scanning Microscopy
2.7. Analysis of Colocalization between SiO2 Particles and Lysosomes
2.8. Flow Cytometry
2.9. Cell Viability/Membrane Rupture Assay
2.10. Enzyme-Linked Immunoassay (ELISA)
2.11. Statistical Analysis
3. Results
3.1. Characterization of Silica Particles
3.2. J774A.1 Macrophages Change Morphology after LPS Stimulation
3.3. Cytotoxicity of SiO2 Particles after Prestimulation of Cells with LPS
3.4. LPS Prestimulated J774A.1 Macrophages Show an Increase in TNF-α Secretion but No Elevation in the Presence of Particles
3.5. Cellular Distribution of SiO2 Particles in LPS Prestimulated J774A.1 Macrophages
3.6. Distinct Intracellular Localization of Different-Sized SiO2 Particles in LPS Prestimulated J774A.1 Macrophages
3.7. Increased Uptake of Both SiO2 Particles after LPS Prestimulation in J774A.1 Macrophages
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Particles | TEM 6 | DLS 7 | c 5 (mg/mL) | |||
---|---|---|---|---|---|---|
Milli-Q | cRPMI | |||||
dc 1 (nm) | dh 2 (nm) | PDI 3 | Ζ 4 (mV) | dh 2 (nm) | ||
SiO2-Cy5 | 920 ± 88 | 908 ± 13 | 0.03 | −44 ± 1 | 931 ± 19 | 143 |
SiO2-BDP FL | 59 ± 6 | 76 ± 1 | 0.16 | −52 ± 2 | 87 ± 6 | 9 |
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Susnik, E.; Taladriz-Blanco, P.; Drasler, B.; Balog, S.; Petri-Fink, A.; Rothen-Rutishauser, B. Increased Uptake of Silica Nanoparticles in Inflamed Macrophages but Not upon Co-Exposure to Micron-Sized Particles. Cells 2020, 9, 2099. https://0-doi-org.brum.beds.ac.uk/10.3390/cells9092099
Susnik E, Taladriz-Blanco P, Drasler B, Balog S, Petri-Fink A, Rothen-Rutishauser B. Increased Uptake of Silica Nanoparticles in Inflamed Macrophages but Not upon Co-Exposure to Micron-Sized Particles. Cells. 2020; 9(9):2099. https://0-doi-org.brum.beds.ac.uk/10.3390/cells9092099
Chicago/Turabian StyleSusnik, Eva, Patricia Taladriz-Blanco, Barbara Drasler, Sandor Balog, Alke Petri-Fink, and Barbara Rothen-Rutishauser. 2020. "Increased Uptake of Silica Nanoparticles in Inflamed Macrophages but Not upon Co-Exposure to Micron-Sized Particles" Cells 9, no. 9: 2099. https://0-doi-org.brum.beds.ac.uk/10.3390/cells9092099