MitoTEMPOL Inhibits ROS-Induced Retinal Vascularization Pattern by Modulating Autophagy and Apoptosis in Rat-Injected Streptozotocin Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Housing and Handling of the Animals
2.2. MitoTEMPOL and STZ Dose
2.3. Fundus Photography and Average Numbers of Retinal Vessel
2.4. Fasting Blood Glucose
2.5. Real-Time PCR Studies
2.6. Western Blot Analysis
2.7. Statistical Analysis
3. Results
3.1. Effect of STZ Injection and MitoTEMPOL on Body Weight, Fasting Blood Glucose Level, Food Intake, and Water Intake
3.2. Effect of STZ Injection and MitoTEMPOL on Funduscopy and Vessel Diameter of the Retina
3.3. Effect of STZ Injection and MitoTEMPOL on SOD and Autophagy Gene Expression
3.4. Effect of STZ Injection and MitoTEMPOL on Carbonyl and Caspase Protein Levels
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Symbol | Primer Sequence (5′ to 3′) Upper Strand: Sense Lower Strand: Antisense | Product Size (bp) | References |
---|---|---|---|
SOD2 | AATGTTGTGTCGGGCGGCGT | 173 bp | [28] |
AGGTCGCGTGGTGCTTGCTG | |||
LC3 | CATGCCGTCCGAGAAGACCT | 70 bp | [29] |
GATGAGCCGGACATCTTCCACT | |||
p62 | CGGAAGTCAGCAAACC | 149 bp | [30] |
ATGCGTCCAGTCGTCA | |||
GAPDH | AGGTCGGTGTGAACGGATTTG | 123 bp | [31] |
TGTAGACCATGTAGTTGAGGTCA |
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Virgana, R.; Atik, N.; Gunadi, J.W.; Jonathan, E.; Ramadhani, D.E.; Soetadji, R.S.; Goenawan, H.; Lesmana, R.; Kartasasmita, A. MitoTEMPOL Inhibits ROS-Induced Retinal Vascularization Pattern by Modulating Autophagy and Apoptosis in Rat-Injected Streptozotocin Model. Life 2022, 12, 1061. https://0-doi-org.brum.beds.ac.uk/10.3390/life12071061
Virgana R, Atik N, Gunadi JW, Jonathan E, Ramadhani DE, Soetadji RS, Goenawan H, Lesmana R, Kartasasmita A. MitoTEMPOL Inhibits ROS-Induced Retinal Vascularization Pattern by Modulating Autophagy and Apoptosis in Rat-Injected Streptozotocin Model. Life. 2022; 12(7):1061. https://0-doi-org.brum.beds.ac.uk/10.3390/life12071061
Chicago/Turabian StyleVirgana, Rova, Nur Atik, Julia Windi Gunadi, Evelyn Jonathan, Dona Erisa Ramadhani, Ray Sebastian Soetadji, Hanna Goenawan, Ronny Lesmana, and Arief Kartasasmita. 2022. "MitoTEMPOL Inhibits ROS-Induced Retinal Vascularization Pattern by Modulating Autophagy and Apoptosis in Rat-Injected Streptozotocin Model" Life 12, no. 7: 1061. https://0-doi-org.brum.beds.ac.uk/10.3390/life12071061