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Article

Short- and Long-Term Mortalities of Small and Large Larvae of Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) on Concrete Surfaces Treated with Three Insecticides: Impact of Food

by
Nickolas G. Kavallieratos
*,
Erifili P. Nika
,
Anna Skourti
,
Constantin S. Filintas
and
Theofania D. Goumenou
Laboratory of Agricultural Zoology and Entomology, Department of Crop Science, Agricultural University of Athens, 75 Iera Odos Str., 11855 Athens, Attica, Greece
*
Author to whom correspondence should be addressed.
Insects 2022, 13(4), 366; https://0-doi-org.brum.beds.ac.uk/10.3390/insects13040366
Submission received: 12 March 2022 / Revised: 28 March 2022 / Accepted: 5 April 2022 / Published: 8 April 2022
(This article belongs to the Collection Stored-Product Pests: Biology, Ecology, Behavior and Integrated Management)
Versions Notes

Abstract

:

Simple Summary

In this study, we used etofenprox, deltamethrin, and the combination of piperonyl butoxide+acetamiprid+d-tetramethrin as surface treatments on concrete (with or without food) to evaluate the short- and long-term mortalities of the lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) small and large larvae. Concerning short-term mortality, etofenprox killed 97.8% and 80.0% of the small and large larvae, respectively, 7 days post-exposure on concrete without food. Deltamethrin caused complete (100.0%) mortality to small larvae without food (3 days post-exposure), small larvae with food, and large larvae without food (5 days post-exposure), and 98.9% mortality to large larvae with food after 5 days of exposure. Piperonyl butoxide+acetamiprid+d-tetramethrin killed all small larvae without food 5 days post-exposure. Concerning long-term mortality, all small larvae exposed to etofenprox died on concrete without food, while piperonyl butoxide+acetamiprid+d-tetramethrin caused 85.0% mortality to small larvae on concrete with food. Overall, deltamethrin was the most efficient active ingredient for the management of both small and large larvae of A. diaperinus.

Abstract

The lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) is an important stored-product pest for the poultry industry as it is a vector of dangerous pathogens for humans. In the present study, we evaluated the short- and long-term mortalities of small and large larvae of A. diaperinus when they were exposed to concrete-covered Petri dishes treated with etofenprox, deltamethrin, and the combination of piperonyl butoxide+acetamiprid+d-tetramethrin. Small and large larvae were exposed to each insecticide applied on concrete surfaces with or without food. The short-term mortality was recorded after 1 day, 3 days, 5 days, and 7 days, while the long-term mortality was recorded 7 days after the transport of the larvae alive to pesticide-free concrete-covered dishes. Regarding short-term mortality levels, 97.8% and 80.0% of the small and large larvae, that were exposed to etofenprox without food, died after 7 days of exposure, respectively. Concerning deltamethrin, all tested small larvae were killed after 3 days (without food) and 5 days (with food) of exposure. For large larvae, deltamethrin caused 98.9% (with food) and 100.0% (without food) mortality levels after 5 days of exposure. The combination of piperonyl butoxide+acetamiprid+d-tetramethrin caused high mortality levels to small larvae, i.e., 84.4% and 100.0% on dishes with and without food, respectively, but low to moderate mortality levels to large larvae that did not exceed 67.8% after 7 days of exposure. Long-term mortality varied vastly among the tested insecticides. Etofenprox killed 100.0% of the small larvae on concrete without food, but 24.0% of the large larvae exposed to concrete containing food. Deltamethrin did not provide long-term mortality to large larvae when food was present. Piperonyl butoxide+acetamiprid+d-tetramethrin caused mortality rates that overall varied from 33.5% (large larvae on concrete with food) to 85.0% (small larvae on concrete with food). In conclusion, deltamethrin killed almost all exposed larvae at exposures of ≤5 days, regardless of their size and the presence of food on the concrete.

1. Introduction

The lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae), is a secondary pest of stored products and a scavenger that occurs globally [1,2]. It infests 77 commodities such as nuts, cereals, vegetables, tobacco, chocolate, and animal products [2,3]. It has been observed eating dead birds and mice [1,4]. Usually, it can be found at henhouses, poultry houses, stables, grain bins, mills, and warehouses [2,3]. Adults are black, oval, and 5–7 mm long [1,2,5], while larvae are brown, elateriform, and approximately 15 mm long [1]. This species is of major importance concerning public health, since both adult and larvae are vectors of multiple pathogens such as Salmonella enterica ssp. enterica (ex Kauffmann and Edwards, 1952) Le Minor and Popoff, 1987 serovars Enteritidis and Typhimurium (Enterobacterales: Enterobacteriaceae), and Campylobacter jejuni (Jones et al., 1931) Veron and Chatelain, 1973 (Campylobacterales: Campylobacteraceae) [6,7]. These pathogens can cause severe symptoms to humans such as diarrhea, bloody diarrhea, fever, abdominal cramps, and vomiting [8,9,10]. Interestingly, A. diaperinus larvae transmit S. enterica ssp. enterica serovar Enteritidis more efficiently than the adults, as chicks that consumed infested larvae had higher bacterial concentrations on their spleen, liver, and cecum than chicks that consumed infected adults [11]. Therefore, the effective management of this species, especially at poultry houses, is highly important.
The larvae of A. diaperinus have become resistant to several chemical insecticides over time [12,13,14] due to their continuous use. Kaufman et al. [15] found that tetrachlorvinphos 500 g/kg sprayed on plywood panels killed only 55.7% of the exposed larvae. Later, Chernaki-Leffer et al. [16] documented the resistance of A. diaperinus to cypermethrin, dichlorvos, and triflumuron. Approximately 208,136 ng cypermethrin/cm2, 179,366 ng dichlorvos/cm2, and 1534 ng triflumuron/cm2 were needed to kill 90% of the exposed A. diaperinus population. Furthermore, 8265.5 ppm cypermethrin dust was needed to kill 90% of A. diaperinus larvae [17]. When larvae were exposed to wood shavings treated with the label dose of cypermethrin dust (0.0573 g/cm2), mortality levels did not exceed 50% [17]. After 7 days of exposure to a treated poultry bedding with an insecticidal combination of citronellal, cypermethrin, and chlorpyrifos, 59.26% of larvae were killed [18]. A recent study showed that alpha cypermethrin, spinosad, and pirimiphos methyl were not effective against A. diaperinus larvae [19]. The management of A. diaperinus larvae faces difficulties when alternative insecticides such as essential oils (EOs) are used [20]. For example, the northern white cedar, Thuja occidentalis L. (Pinales: Cupressaceae), and the common tansy, Tanacetum vulgare L. (Asterales: Asteraceae) EOs, as well as their main constituents, i.e., α-thujone and β-thujone, caused low mortality levels to A. diaperinus larvae, not exceeding 30% [20]. Tomasi et al. [21] reported that 0.4 mg/mL glycerol monolaurate killed <30% of the exposed larvae in Petri dishes containing treated filter papers, 8 days post-exposure.
Etofenprox [2-(4-ethoxyphenyl)-2-methylpropyl-3-phenoxybenzyl ether] (supercooled liquid) is a pyrethroid insecticide that affects the neurotransmission through the prolonged activation of the sodium channels [22]. It is characterized as an ingestion and contact insecticide with light stability and over 3 months half-life [22,23,24]. Deltamethrin [(s)-ca-cyano-m-phenoxybenzyl(1R,3R)-3-(2,2-dibromovinyl)-2,2-dimethylcyclo-propane-carboxylate] is also a pyrethroid insecticide that has a similar mode of action with etofenprox, by causing prolonged activation of the neuronal membranes’ sodium channels [25]. Piperonyl butoxide (3,4-methylenedioxy-6-propylbenxyl n-butyl diethyleneglycol ether) is an aliphatic-aromatic polyether that acts as an insecticide synergist [26]. It has long stability (over 18 years), enhances the insecticidal properties of other insecticides, and inhibits the detoxification activity of the insects by reducing the cytochrome P450 levels [26,27,28]. Acetamiprid [N-[(6-chloropyridin-3-yl)methyl]-N′-cyano-N-methylethanimidamide] is a neonicotinoid insecticide that acts on the nicotinic acetylcholine receptors (nAChR) of insects, resulting in their excitation, paralysis, and death [29]. Lastly, d-tetramethrin [3,4,5,6-tetrahydrophthalimidomethyl (1RS)-cis-trans-chrysanthemate] is a pyrethroid insecticide with a similar mode of action to etofenprox and deltamethrin, i.e., it attacks the insects’ nervous system by acting on the function of sodium channels [23,30,31,32].
Even though many insecticidal formulations have been tested against A. diaperinus larvae, etofenprox, deltamethrin, and the combination of piperonyl butoxide+acetamiprid+d-tetramethrin have never been evaluated as surface treatments for the management of this species. After a meticulous search of the published literature, only the combination of piperonyl butoxide+chlorpyrifos has been previously tested in contact toxicity assays [33]. Therefore, the objectives of the current study were to evaluate the short- and long-term mortalities of etofenprox, deltamethrin, and the combination of piperonyl butoxide+acetamiprid+d-tetramethrin on concrete against small and large larvae of A. diaperinus.

2. Materials and Methods

2.1. Insects and Food

Coleopteran individuals were cultured at 30 °C, 55% relative humidity, and in total darkness [34,35] at the Laboratory of Agricultural Zoology and Entomology, Agricultural University of Athens. The founding individuals were obtained from a laboratory colony that was kept in the Laboratory of Agricultural Entomology (Benaki Phytopathological Institute, Kifissia, Attica, Greece). For the experimentation, unsexed small larvae (<7 mm) and large larvae (>7 mm) were used [1]. The rearing medium was wheat bran (75%) and yeast (25%), with apple cuttings for extra moisture [35]. The rearing medium was used as food in the experiments. Diet moisture was 12.3%, as determined by a calibrated moisture meter (mini GAC plus, Dickey-John Europe S.A.S., Colombes, France).

2.2. Formulations

The formulations that were used in the current experiments were: Phobi E EC with 300 gr/l etofenprox active ingredient (a.i.) (provided by Protecta, Peristeri, Attica, Greece), K-Othrine WG with 25% deltamethrin (a.i.) (provided by Bayer Hellas, Amaroussion, Attica, Greece), and Dobol® EC with 10% piperonyl butoxide, 5% w/v acetamiprid and 2.5% w/v d-tetramethrin (a.i.) (provided by Société Kwizda France, Marly le Roi, France).

2.3. Bioassays

The formulations were applied at commercially labeled doses for surface applications. Consequently, etofenprox was tested at 0.015 mgr (a.i.)/cm2, deltamethrin at 0.0025 mg (a.i.)/cm2, and piperonyl butoxide+acetamiprid+d-tetramethrin at 0.0002 mL formulation/cm2. For the experiment, we prepared three replicates (with each replicate having 3 subreplicates) of Petri dishes (8 cm diameter and 1.5 cm high). Each subreplicate was constituted by 10 dishes, containing one individual each, to ensure that the insects will not cannibalize each other [36]. The surface area of each dish was 50.27 cm2. The bottoms of the dishes were covered by concrete (CEM I 52.5 N material, Durostick, Aspropyrgos, Attica, Greece) 24 h before the beginning of the experiments. The lids of all the dishes had a hole (1.5 cm diameter) covered with gauze to aerate the internal space of the dishes. Polytetrafluoroethylene (60 wt% dispersion in water) (Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany) was applied to the inside vertical part of the dishes to ensure the enclosure of the tested individuals in the dishes. Each concrete-covered dish was treated with an aqueous solution of total volume of 1 mL with the proper concentration of each tested formulation. The fine mist was achieved by using a separate airbrush (AG-4 Mecafer S.A., Valence, France) for each formulation. Thereupon, we weighted 0.5 g quantities of the diet with an electronic balance (Precisa XB3200D, Alpha Analytical Instruments, Gerakas, Attica, Greece) that were transferred into each concrete-covered dish. Additional series of dishes were prepared with the exact same procedure, but diet was not added. Furthermore, extra series of dishes were produced as controls, with or without food, but this time they were sprayed with distilled water with a different airbrush. In each prepared dish a single small or large larva was inserted. Then, the dishes were put into incubators set at 30 °C and 55% relative humidity and total darkness. The mortality of both small and large larvae was counted under a stereomicroscope (Olympus SZX9, Bacacos S.A., Athens, Greece) with 57× magnification, 1 day, 3 days, 5 days, and 7 days post-exposure, with a fine brush (Cotman 111 No 000, Winsor and Newton, London, UK) by nudging gently the tested individuals. If no movement was detected, individuals were considered dead. The individuals that were alive were transferred to new untreated concrete-covered dishes after 7 days of exposure, to evaluate their long-term mortality. For the larvae that had food into the dishes, new quantities of 0.5 g diet were put into the new untreated dishes. After 7 days, the dead individuals were also counted as described above.

2.4. Data Analysis

Short- and long-term mortalities of the controls was low (<5%), consequently no correction was applied to the data. Prior to the analysis, data were log (x + 1) converted to normalize the variance [37,38]. The repeated-measures model was used to run the analysis, separately for each larval size group [39]. In the analysis, exposure was the repeated factor. Mortality was the response variance. Insecticide and presence/absence of food were the main effects. In the analysis, the associated interactions of the main effects were considered. Data for long-term mortality were submitted to a two-way ANOVA, separately for each size of larval group. Mortality was the response variable. Insecticide and the presence/absence of food were the main effects. Means were separated using the Tukey-Kramer honest significant difference (HSD) test at the 0.05 significance level [40]. The JMP 14 software was used for all analyses [41].

3. Results

3.1. Short- and Long-Term Mortalities of Alphitobius Diaperinus Small Larvae

All main effects and their associated interactions, between and within exposure intervals, were significant (Table 1). Etofenprox caused elevated short-term mortality to A. diaperinus small larvae (97.8%) after 7 days of exposure without food (Table 2). When food was present on concrete, mortality rates were significantly lower than when food was absent at all exposure intervals, not exceeding 64.4% at the end of the experimental period. Complete mortality (100.0%) was achieved when concrete was treated with deltamethrin without containing food after 3 days of exposure and after 5 days of exposure on concrete when food was existed (Table 3). After 1 day of exposure, mortality levels were not significantly different with or without food (46.7% and 75.6% mortality, respectively). Concerning piperonyl butoxide+acetamiprid+d-tetramethrin, complete mortality was achieved when food was absent, that differed significantly when food existed (60.0%), 5 days post-exposure (Table 4). Yet, when food was present, 84.4% of the exposed larvae were dead, after 7 days of exposure. Long-term mortality was moderate on concrete treated with etofenprox with the presence of food (64.1%) and complete on concrete without food (100.0%), but they were not statistically different (Table 5). Piperonyl butoxide+acetamiprid+d-tetramethrin killed 85.0% of the remaining individuals on concrete with food at the end of the experimental period.

3.2. Short- and Long-Term Mortalities of Alphitobius Diaperinus Large Larvae

All main effects and their associated interactions were significant between and within exposure intervals (Table 1). Regarding short-term mortality, etofenprox killed significantly fewer exposed individuals (37.8%) with the presence of food than without food (80.0%), 7 days post exposure (Table 2). Complete mortality was documented on concrete treated with deltamethrin without food while 98.9% was the achieved mortality on concrete with food after 5 days of exposure (Table 3). Piperonyl butoxide+acetamiprid+d-tetramethrin caused low to moderate mortality at large larvae, not exceeding 13.3% (on concrete with food) and 67.8% (on concrete without food), that was significantly higher, 7 days post-exposure (Table 4). Concerning long-term mortality, etofenprox resulted in low (24.0%) and moderate (53.7%) mortality levels, with and without food, respectively (Table 5). No long-term mortality was recorded on concrete treated with deltamethrin with the presence of food. Significantly more large larvae died on concrete without food (68.2%) than on concrete with food (33.5%) in the case of piperonyl butoxide+acetamiprid+d-tetramethrin.

4. Discussion

The results of this study have revealed that etofenprox, deltamethrin, and the combination of piperonyl butoxide+acetamiprid+d-tetramethrin are effective against both small and large larvae of A. diaperinus, with or without food. Previous knowledge has revealed that larvae of A. diaperinus cannot be easily managed. For example, in a field study, Hess et al. [42] examined six insecticidal treatments against larvae of this species. They found low reduction of the larval individuals at five out of the six scenarios. Steelman [43], by applying 450.41 mg/g cyfluthrin or 489.75 mg/g methoxychlor to the prosternum of each larva, caused 50% mortality. To achieve 90.0% of larval mortality, 112.6 ppm cyfluthrin or 132.0 ppm of the imidacloprid were needed [44]. After 7 days of exposure to dishes covered with a biocidal paint containing ultramarine+permethrin+violet 23, 41.33% of the larvae were killed, while 82.67% mortality was achieved 14 days post-exposure [45]. Similarly, filter paper treated with 1% EO, 1% EO-nanoemulsion (NE), and 1% EO-nanocapsules (NC) of the true cinnamon tree, Cinnamomum verum Jan Svatopluk Presl (J.Presl) [= Cinnamomum zeylanicum Blume] (Laurales: Lauraceae), caused approximately 50%, 40%, and 20% mortality, respectively, after 7 days of exposure [46]. In a recent study, the mortality caused by pirimiphos-methyl, spinosad, and alpha-cypermethrin against A. diaperinus larvae exposed to treated concrete did not exceed 40.0%, with or without food [19].
The strain of the exposed A. diaperinus individuals should be taken into account since different strains are more or less susceptible to certain insecticides. Cyfluthrin, permethrin, permethrin+acetamiprid, and pirimiphos-methyl were examined against twelve A. diaperinus strains [47]. Among these strains, three of them exhibited pyrethroid resistance, while five of them were susceptible to all insecticides. According to Hichmann et al. [48], nine geographical strains of this species exhibited variable resistance ratios ranging between 1.0-fold and 17.0-fold (cypermethrin) and between 1.5-fold and 20.5-fold (chlorpyrifos). Due to the above resistance issues identified on several A. diaperinus strains, multiple a.i. could be an efficient way to manage this species [49].
At all experiments on concrete surfaces with food, mortality levels were lower than when food was absent, for small and large larvae, for both short- and long-term mortalities. This observation indicates that the presence of food plays an important role for the effectiveness of a.i. because food degrades and/or absorbs part of the treated insecticides, acting as a barrier between the treated surface and the tested individuals. In previous experiments, the efficacy of methoprene alpha-cypermethrin, chlorfenapyr, spinosad, deltamethrin, pirimiphos-methyl, cyfluthrin, and pyriproxyfen decreased when food was present on concrete surfaces [19,50,51,52,53,54]. Moreover, food can impede insects from contacting the treated concrete surfaces, increasing the percentage of the surviving individuals [50], and can also absorb a part of the insecticide, which could lead to the death of the feeding insect individuals [55]. Toews et al. [56] reported that the increased amount of food on the treated plastic surfaces resulted in a decrease in the mortality rate of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Our findings showed that deltamethrin was highly effective either with the presence or the absence of food. This is an important finding since previous studies have documented that the presence of food on deltamethrin treated surfaces can moderate the efficacy of this insecticide. For example, Velki et al. [57] found that for 10%, 50% and 90% T. castaneum mortality, higher doses of deltamethin were needed at treatments with flour than at treatments without flour. Similarly, Boukouvala and Kavallieratos [54] showed that more khapra beetle, Trogoderma granarium Everts (Coleoptera: Dermestidae) eggs hatched on treated concrete surfaces with food than on without food. Additionally, mortality levels were higher on concrete surfaces without food. Apparently, the performance of deltamethrin depends on the exposed species when surface treatments is followed as a management strategy. Whether A. diaperinus adults are subject to the same pattern merits further investigation.
Our results reveal that small larvae were more susceptible than large larvae. This issue could be attributed to the fact that different age larvae have cuticle with various thickness, softness and epicuticular lipid composition [58,59,60,61]. Additionally, large larvae are less vigorous than small larvae since they are close to becoming pupae [60], and therefore move less on the treated surface leading to lower mortality levels. In a historic study, Gast [62] found that the more a larva weighs the more insecticide is needed to kill it. Since small and large larvae are exposed to the same dose of toxicant, it is expected that small larvae will die more easily. This trend has been previously documented by several researchers [61,63,64]. Small A. diaperinus larvae reached 85.0% mortality, while only 7.4% of large larvae were killed by 12.5 mg/mL fruit extract of the star anise, Illicum verum Hook. F. (Austrobaileyales: Schisandraceae), 5 days post-exposure [65]. Kavallieratos et al. [61] noticed that small larvae of the larger mealworm, Tenebrio molitor L. (Coleoptera: Tenebrionidae), were more susceptible than large larvae when they were exposed to three types of grains treated with deltamethrin, pirimiphos-methyl, silicoSec, and spinosad, at all exposure intervals. Similarly, Kavallieratos et al. [63] and Arthur et al. [64] showed that large larvae of T. granarium were harder to kill than small larvae when they were exposed to surfaces treated with pirimiphos-methyl, chlorfenapyr, deltamethrin, spinosad, pyriproxyfen, and cyfluthrin. The combination of piperonyl butoxide+acetamiprid+d-tetramethrin was highly effective against small larvae of T. granarium on concrete, although there are no comparative data with large larvae, since 84.4% of the exposed individuals died 7 days post-treatment at 0.0002 mL formulation/cm2 [66].

5. Conclusions

To conclude, the current study sheds light on the efficacy of etofenprox, deltamethrin, and the combination of piperonyl butoxide+acetamiprid+d-tetramethrin against A. diaperinus small and large larvae on treated concrete surfaces with the presence or absence of food, under short exposure intervals. The most efficient insecticide was deltamethrin as it killed almost all exposed small and large larvae, 5 days post-exposure on concrete with or without food. Etofenprox and piperonyl butoxide+acetamiprid+d-tetramethrin caused elevated short- and long-term mortality levels, but they depend on the presence or absence of food and the size of the larvae. Further experimentation is needed to fully unravel the potentials of the tested insecticides applied on different types of surfaces, e.g., different types of storage, plastic, wood, tile, on several food commodities, and against different developmental stages of A. diaperinus.

Author Contributions

Conceptualization, N.G.K.; Methodology, N.G.K., E.P.N. and A.S.; Validation, N.G.K.; Formal Analysis, N.G.K. and E.P.N.; Investigation, N.G.K., E.P.N., A.S., C.S.F. and T.D.G.; Resources, N.G.K.; Data Curation, N.G.K., E.P.N. and A.S.; Writing—Original Draft Preparation, N.G.K. and E.P.N.; Writing—Review and Editing, N.G.K., E.P.N., A.S., C.S.F. and T.D.G.; Visualization, N.G.K.; Supervision, N.G.K.; Project Administration, N.G.K.; Funding Acquisition, N.G.K. All authors have read and agreed to the published version of the manuscript.

Funding

The research was partially funded by the 34.0889 project (Special Account for Research Funds of the Agricultural University of Athens).

Institutional Review Board Statement

Not applicable.

Data Availability Statement

Data are contained within the article.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Rees, D. Insects of Stored Products; Manson Publishing: London, UK, 2004. [Google Scholar]
  2. Hagstrum, D.W.; Klejdysz, T.; Subramanyam, B.; Nawrot, J. Atlas of Stored-Product Insects and Mites; AACC International: St. Paul, MN, USA, 2013. [Google Scholar]
  3. Hagstrum, D.W.; Subramanyam, B. Stored-Product Insect Resource; AACC International: St. Paul, MN, USA, 2009. [Google Scholar]
  4. Hill, D.S. Pests of Stored Foodstuffs and Their Control; Kluwer Academic Publishers: New York, NY, USA, 2003. [Google Scholar]
  5. Kumar, R. Insect Pests on Stored Grain. Biology, Behavior, and Management Strategies; Apple Academic Press: Waretown, NJ, USA, 2017. [Google Scholar]
  6. Hazeleger, W.C.; Bolder, N.M.; Beumer, R.R.; Jacobs-Reitsma, W.F. Darkling beetles (Alphitobius diaperinus) and their larvae as potential vectors for the transfer of Campylobacter jejuni and Salmonella enterica serovar Paratyphi B variant Java between successive broiler flocks. Appl. Environ. Microbiol. 2008, 74, 6887–6891. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  7. EUR Lex. Commission Regulation (EU) No 200/2012 of 8 March 2012 Concerning a Union Target for the Reduction of Salmonella enteritidis and Salmonella typhimurium in Flocks of Broilers, as Provided for in Regulation (EC) No 2160/2003 of the European Parliament and of the Council Text with EEA Relevance. 2012. Available online: https://eur-lex.europa.eu/legal-content/EN/ALL/?uri=CELEX%3A32012R0200 (accessed on 11 March 2022).
  8. Jertborn, M.; Haglind, P.; Iwarson, S.; Svennerholm, A.M. Estimation of symptomatic and asymptomatic Salmonella infections. Scand. J. Infect. Dis. 1990, 22, 451–455. [Google Scholar] [CrossRef]
  9. Blaser, M.J. Epidemiologic and clinical features of Campylobacter jejuni infections. J. Infect. Dis. 1997, 176, S103–S105. [Google Scholar] [CrossRef] [Green Version]
  10. Litrup, E.; Torpdahl, M.; Malorny, B.; Huehn, S.; Helms, M.; Christensen, H.; Nielsen, E.M. DNA microarray analysis of Salmonella serotype Typhimurium strains causing different symptoms of disease. BMC Microbiol. 2010, 10, 96. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  11. Leffer, A.M.; Kuttel, J.; Martins, L.M.; Pedroso, A.C.; Astolfi-Ferreira, C.S.; Ferreira, F.; Ferreira, A.J.P. Vectorial competence of larvae and adults of Alphitobius diaperinus in the transmission of Salmonella Enteritidis in poultry. Vector Borne Zoonotic Dis. 2010, 10, 481–487. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  12. Lambkin, T.A. Investigations into the Management of the Darkling Beetle. A Report for the Rural Industries Research and Development Corporation; RIRDC Publication: Kingston, Australia, 2001. [Google Scholar]
  13. Lambkin, T.A. Baseline responses of adult Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) to fenitrothion, and susceptibility status of populations in Queensland and New South Wales, Australia. J. Econ. Entomol. 2005, 98, 938–942. [Google Scholar] [CrossRef]
  14. Hamm, R.L.; Kaufman, P.E.; Reasor, C.A.; Rutz, D.A.; Scott, J.G. Resistance to cyfluthrin and tetrachlorvinphos in the lesser mealworm, Alphitobius diaperinus, collected from the eastern United States. Pest Manag. Sci. 2006, 62, 673–677. [Google Scholar] [CrossRef]
  15. Kaufman, P.E.; Strong, C.; Rutz, D.A. Susceptibility of lesser mealworm (Coleoptera: Tenebrionidae) adults and larvae exposed to two commercial insecticides on unpainted plywood panels. Pest Manag. Sci. 2008, 64, 108–111. [Google Scholar] [CrossRef]
  16. Chernaki-Leffer, A.M.; Sosa-Gómez, D.R.; Almeida, L.M.; Lopez, I.D.O.N. Susceptibility of Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) to cypermethrin, dichlorvos and triflumuron in southern Brazil. Rev. Bras. Entomol. 2011, 55, 125–128. [Google Scholar] [CrossRef] [Green Version]
  17. Chernaki-Leffer, A.M.; Ishizuca, M.M.; Balieiro, J.C.C.; Gorniak, S.L. The laboratory efficacy of cypermethrin dust against lesser mealworm larvae and adults, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). J. Anim. Vet. Adv. 2012, 11, 2215–2219. [Google Scholar] [CrossRef]
  18. Wolf, J.; Potrich, M.; Lozano, E.R.; Gouvea, A.; Pegorini, C.S. Combined physical and chemical methods to control lesser mealworm beetles under laboratory conditions. Poult. Sci. 2015, 94, 1145–1149. [Google Scholar] [CrossRef] [PubMed]
  19. Zafeiriadis, S.; Sakka, M.K.; Athanassiou, C.G. Efficacy of contact insecticides for the control of the lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). J. Stored Prod. Res. 2021, 92, 101817. [Google Scholar] [CrossRef]
  20. Szołyga, B.; Gniłka, R.; Szczepanik, M.; Szumny, A. Chemical composition and insecticidal activity of Thuja occidentalis and Tanacetum vulgare essential oils against larvae of the lesser mealworm, Alphitobius diaperinus. Entomol. Exp. Appl. 2014, 151, 1–10. [Google Scholar] [CrossRef]
  21. Tomasi, T.; Zortéa, T.; dos Reis, T.R.; Lopes, L.Q.S.; Baldissera, M.D.; Santos, R.C.V.; Baretta, D.; Stefani, L.M.; da Silva, A.S. Insecticidal action of glycerol monolaurate against the lesser mealworm (Alphitobius diaperinus) and its ecotoxicological effect on Enchytraeus crypticus. Acta Sci. Vet. 2018, 46, 1581. [Google Scholar] [CrossRef]
  22. EUR Lex. Regulation (EU) Νο 528/2012 the Biocidal Products Committee of 27 September 2013 concerning the making available on the market and use of biocidal products: Etofenprox, Product Type 18 (Insecticide). Off. J. Eur. Union 2013, L 167, 1–109. [Google Scholar]
  23. Kaneko, H. Pyrethroid chemistry and metabolism. In Haye’s Handbook of Pesticide Toxicology; Krieger, R., Ed.; Elsevier Academic Press Inc.: London, UK, 2011; pp. 1635–1663. [Google Scholar]
  24. Vasquez, M.E.; Holstege, D.M.; Tjeerdema, R.S. Aerobic versus anaerobic microbial degradation of etofenprox in a California rice field soil. J. Agric. Food Chem. 2011, 59, 2486–2492. [Google Scholar] [CrossRef] [PubMed]
  25. Freeborn, D.L.; McDaniel, K.L.; Moser, V.C.; Herr, D.W. Use of electroencephalography (EEG) to assess CNS changes produced by pesticides with different modes of action: Effects of permerthrin, deltamethrin, fipronil, imidacloprid, carbaryl, and triadimefon. Toxicol. Appl. Pharmacol. 2015, 282, 184–194. [Google Scholar] [CrossRef]
  26. Di Blasi, G. A Review of the chemistry of piperonyl butoxide. In Piperonyl Butoxide. The Insecticide Synergist; Jones, D.G., Ed.; Academic Press: London, UK, 1999; pp. 55–70. [Google Scholar]
  27. Farnham, A.W. The mode of action of piperonyl butoxide with reference to studying pesticide resistance. In Piperonyl Butoxide. The Insecticide Synergist; Jones, D.G., Ed.; Academic Press: London, UK, 1999; pp. 199–213. [Google Scholar]
  28. Hodgson, E.; Levi, P.E. Interactions of piperonyl butoxide with cytochrome P450. In Piperonyl Butoxide. The Insecticide Synergist; Jones, D.G., Ed.; Academic Press: London, UK, 1999; pp. 41–55. [Google Scholar]
  29. Ghanim, M.; Ishaaya, I. Insecticides with novel modes of action: Mechanism and resistance management. In Insecticides with Novel Modes of Action: Mechanism and Application; Ishaaya, I., Degheele, D., Eds.; Springer: Berlin/Heidelberg, Germany, 2010; pp. 385–407. [Google Scholar]
  30. EPA (Environmental Protection Agency). Registration Eligibility Decision (RED) Document for Tetramethrin; EPA: Washington, DC, USA, 2008; pp. 1–50.
  31. Soderlund, D.M. Toxicology and mode of action of pyrethroid insecticides. In Haye’s Handbook of Pesticide Toxicology; Krieger, R., Ed.; Elsevier Academic Press Inc.: London, UK, 2011; pp. 1665–1686. [Google Scholar]
  32. Field, L.M.; Davies, T.G.E.; O’Reilly, A.O.; Williamson, M.S.; Wallace, B.A. Voltage-gated sodium channels as targets for pyrethroid insecticides. Eur. Biophys. J. 2017, 46, 675–679. [Google Scholar] [CrossRef] [Green Version]
  33. Yeasmin, A.M.; Waliullah, T.M.; Rahman, A.S. Synergistic effects of chlorpyrifos with piperonyl butoxide (pbo) against the lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). Asian Pac. J. Reprod. 2014, 3, 305–310. [Google Scholar] [CrossRef]
  34. Renault, D.; Nedved, O.; Hervant, F.; Vernon, P. The importance of fluctuating thermal regimes for repairing chill injuries in the tropical beetle Alphitobius diaperinus (Coleoptera: Tenebrionidae) during exposure to low temperature. Physiol. Entomol. 2004, 29, 139–145. [Google Scholar] [CrossRef]
  35. Rice, S.J.; Lambkin, T.A. A new culture method for lesser mealworm, Alphitobius diaperinus. J. Appl. Entomol. 2009, 133, 67–72. [Google Scholar] [CrossRef]
  36. Szczepanik, M.; Gliszczyńska, A.; Hnatejko, M.; Zawitowska, B. Effects of halolactones with strong feeding-deterrent activity on the growth and development of larvae of the lesser mealworm, Alphitobius diaperinus (Coleoptera: Tenebrionidae). Appl. Entomol. Zool. 2016, 51, 393–401. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  37. Zar, J.H. Biostatistical Analysis; Pearson Education Limited: Essex, UK, 2014. [Google Scholar]
  38. Scheff, D.S.; Arthur, F.H. Fecundity of Tribolium castaneum and Tribolium confusum adults after exposure to deltamethrin packaging. J. Pest Sci. 2018, 91, 717–725. [Google Scholar] [CrossRef]
  39. Sall, J.; Lehman, A.; Creighton, L. JMP start statistics. In A Guide to Statistics and Data Analysis Using JMP and JMP in Software; Duxbury Press: Belmont, ON, Canada, 2001. [Google Scholar]
  40. Sokal, R.R.; Rohlf, F.J. Biometry; Freeman & Company: New York, NY, USA, 1995. [Google Scholar]
  41. SAS Institute Inc. Using JMP 14; SAS Institute Inc.: Cary, NC, USA, 2018. [Google Scholar]
  42. Hess, J.B.; Macklin, K.S.; McCrea, B.A. Litter beetle (Alphitobius diaperinus Panzer) counts in broiler houses treated with a range of insecticides. J. Appl. Anim. Res. 2008, 33, 127–131. [Google Scholar] [CrossRef]
  43. Steelman, C.D. Comparative susceptibility of adult and larval lesser mealworms, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae), collected from broiler houses in Arkansas to selected insecticides. J. Agric. Urban Entomol. 2008, 25, 111–125. [Google Scholar] [CrossRef]
  44. Singh, N.; Johnson, D. Baseline susceptibility and cross-resistance in adult and larval Alphitobius diaperinus (Coleoptera: Tenebrionidae) collected from poultry farms in Arkansas. J. Econ. Entomol. 2015, 108, 1994–1999. [Google Scholar] [CrossRef]
  45. Dzik, S.; Mituniewicz, T. Effectiveness of biocidal paint containing permethrin, ultramarine and violet 23 against Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) in laboratories and poultry houses. Animals 2020, 10, 1461. [Google Scholar] [CrossRef]
  46. Volpato, A.; Baretta, D.; Zortéa, T.; Campigotto, G.; Galli, G.M.; Glombowsky, P.; Santos, R.C.V.; Quatrin, P.M.; Ourique, A.F.; Baldissera, M.D.; et al. Larvicidal and insecticidal effect of Cinnamomum zeylanicum oil (pure and nanostructured) against mealworm (Alphitobius diaperinus) and its possible environmental effects. J. Asia Pac. Entomol. 2016, 19, 1159–1165. [Google Scholar] [CrossRef]
  47. Renault, D.; Colinet, H. Differences in the susceptibility to commercial insecticides among populations of the lesser mealworm Alphitobius diaperinus collected from poultry houses in France. Insects 2021, 12, 309. [Google Scholar] [CrossRef]
  48. Hichmann, F.; de Morais, A.F.; Bronzatto, E.S.; Giacomelli, T.; Guedes, J.V.C.; Bernardi, O. Susceptibility of the lesser mealworm, Alphitobius diaperinus (Coleoptera: Tenebrionidae), from broiler farms of southern Brazil to insecticides. J. Econ. Entomol. 2018, 111, 980–998. [Google Scholar] [CrossRef]
  49. Opit, G.; Collins, P.J.; Daglish, G.J. Resistance management. In Stored Product Protection; Hagstrum, D.W., Phillips, T.W., Cuperus, G., Eds.; Kansas State University: Manhattan, KS, USA, 2012; pp. 143–156. [Google Scholar]
  50. Arthur, F.H. Impact of accumulated food on survival of Tribolium castaneum on concrete treated with cyfluthrin wettable powder. J. Stored Prod. Res. 2000, 36, 15–23. [Google Scholar] [CrossRef]
  51. Arthur, F.H. Residual efficacy of aerosols to control Tribolium castaneum and Tribolium confusum. In Proceedings of the 10th International Working Conference on Stored Product Protection, Estoril, Portugal, 27 June–2 July 2010; Carvalho, M.O., Fields, P.G., Adler, C.S., Arthur, F.H., Athanassiou, C.G., Campbell, J.F., Fleurat-Lessard, F., Flinn, P.W., Hodges, R.J., Isikber, A.A., et al., Eds.; Julius Kühn-Institut: Berlin, Germany, 2010; pp. 789–792. [Google Scholar]
  52. Arthur, F.H. Food source effect and residual efficacy of chlorfenapyr as a surface treatment on sealed and unsealed concrete. J. Stored Prod. Res. 2015, 64, 65–71. [Google Scholar] [CrossRef]
  53. Athanassiou, C.G.; Arthur, F.H.; Kavallieratos, N.G.; Throne, J.E. Efficacy of pyriproxyfen for control of stored-product psocids (Psocoptera) on concrete surfaces. J. Econ. Entomol. 2011, 104, 1765–1769. [Google Scholar] [CrossRef] [PubMed]
  54. Boukouvala, M.C.; Kavallieratos, N.G. Effect of six insecticides on egg hatching and larval mortality of Trogoderma granarium Everts (Coleoptera: Dermestidae). Insects 2020, 11, 263. [Google Scholar] [CrossRef]
  55. Arthur, F.H.; Domingue, M.J.; Scheff, D.S.; Myers, S.W. Bioassays and methodologies for insecticide tests with larvae of Trogoderma granarium (Everts), the khapra beetle. Insects 2019, 10, 145. [Google Scholar] [CrossRef] [Green Version]
  56. Toews, M.D.; Campbell, J.F.; Arthur, F.H. The presence of flour affects the efficacy of aerosolized insecticides used to treat the red flour beetle, Tribolium castaneum. J. Insect Sci. 2010, 10, 196. [Google Scholar] [CrossRef] [Green Version]
  57. Velki, M.; Plavšin, I.; Dragojević, J.; Hackenberger, B.K. Toxicity and repellency of dimethoate, pirimiphos-methyl and deltamethrin against Tribolium castaneum (Herbst) using different exposure methods. J. Stored Prod. Res. 2014, 59, 36–41. [Google Scholar] [CrossRef]
  58. Armold, M.T.; Blomquist, G.J.; Jackson, L.L. Cuticular lipids of insects III. The surface lipids of aquatic and terrestrial life forms of the big stonefly Pteronarcys california (Newport). Comp. Biochem. Physiol. 1969, 31, 685–692. [Google Scholar] [CrossRef]
  59. Mewis, I.; Ulrichs, C. Action of amorphous diatomaceous earth against different stages of the stored product pests Tribolium confusum, Tenebrio molitor, Sitophilus granarius and Plodia interpunctella. J. Stored Prod. Res. 2001, 37, 153–164. [Google Scholar] [CrossRef]
  60. Vayias, B.J.; Athanassiou, C.G. Factors affecting the insecticidal efficacy of the diatomaceous earth formulation silicoSec against adults and larvae of the confused flour beetle, Tribolium confusum DuVal (Coleoptera: Tenebrionidae). Crop Prot. 2004, 23, 565–573. [Google Scholar] [CrossRef]
  61. Kavallieratos, N.G.; Michail, E.J.; Boukouvala, M.C.; Nika, E.P.; Skourti, A. Efficacy of pirimiphos-methyl, deltamethrin, spinosad and silicoSec against adults and larvae of Tenebrio molitor L. on wheat, barley and maize. J. Stored Prod. Res. 2019, 83, 161–167. [Google Scholar] [CrossRef]
  62. Gast, R.T. The relationship of weight of lepidopterous larvae to effectiveness of topically applied insecticides. J. Econ. Entomol. 1959, 52, 1115–1117. [Google Scholar] [CrossRef]
  63. Kavallieratos, N.G.; Athanassiou, C.G.; Barda, M.S.; Boukouvala, M.C. Efficacy of five insecticides for the control of Trogoderma granarium Everts (Coleoptera: Dermestidae) larvae on concrete. J. Stored Prod. Res. 2016, 66, 18–24. [Google Scholar] [CrossRef]
  64. Arthur, F.H.; Ghimire, M.N.; Myers, S.W.; Phillips, T.W. Evaluation of pyrethroid insecticides and insect growth regulators applied to different surfaces for control of Trogoderma granarium (Coleoptera: Dermestidae) the khapra beetle. J. Econ. Entomol. 2018, 111, 612–619. [Google Scholar] [CrossRef] [Green Version]
  65. Szcezepanik, M.; Szumny, A. Insecticidal activity of star anise (Illicum verum Hook. F.) fruits extracts against lesser mealworm, Alphitobius diaperinus Panzer (Coleoptera: Tenebrionidae). Allelopath. J. 2011, 27, 277–288. [Google Scholar]
  66. Kavallieratos, N.G.; Boukouvala, M.C. Efficacy of d-tetramethrin and acetamiprid for control of Trogoderma granarium Everts (Coleoptera: Dermestidae) adults and larvae on concrete. J. Stored Prod. Res. 2019, 80, 79–84. [Google Scholar] [CrossRef]
Table
Table 1. MANOVA parameters for main effects and associated interactions for mortality of Alphitobius diaperinus small and large larvae between and within exposure intervals (error DF = 48).
Table 1. MANOVA parameters for main effects and associated interactions for mortality of Alphitobius diaperinus small and large larvae between and within exposure intervals (error DF = 48).
Effect Small LarvaeLarge Larvae
Between exposure intervals
SourceDFFpFp
Intercept14233.7<0.012939.7<0.01
Insecticide230.8<0.01170.5<0.01
Food135.1<0.01169.7<0.01
Insecticide × food28.6<0.0130.8<0.01
Within exposure intervals
Exposure389.1<0.01222.9<0.01
Exposure × insecticide619.1<0.0118.2<0.01
Exposure × food318.1<0.018.4<0.01
Exposure × insecticide × food64.4<0.0112.0<0.01
Table
Table 2. Mean short-term mortality (% ± SE) of Alphitobius diaperinus small and large larvae exposed to concrete treated with etofenprox for 1 day, 3 days, 5 days, and 7 days. Within each row, means followed by the same uppercase letter are not significantly different (in all cases DF = 3, 35, Tukey-Kramer HSD test at p = 0.05). Within each column, means that are followed by the same lower-case letter are not significantly different (in all cases DF = 3, 35, Tukey-Kramer HSD test at p = 0.05).
Table 2. Mean short-term mortality (% ± SE) of Alphitobius diaperinus small and large larvae exposed to concrete treated with etofenprox for 1 day, 3 days, 5 days, and 7 days. Within each row, means followed by the same uppercase letter are not significantly different (in all cases DF = 3, 35, Tukey-Kramer HSD test at p = 0.05). Within each column, means that are followed by the same lower-case letter are not significantly different (in all cases DF = 3, 35, Tukey-Kramer HSD test at p = 0.05).
Larval Group1 Day3 Days5 Days7 DaysFp
Small larvae (with food)5.6 ± 1.8 Cb18.9 ± 5.1 BCb28.9 ± 4.2 ABb64.4 ± 8.4 Ab11.0<0.01
Small larvae (without food)25.6 ± 1.8 Ca57.8 ± 4.9 Ba82.2 ± 4.7 Aa97.8 ± 1.5 Aa97.5<0.01
Large larvae (with food)0.0 ± 0.0 Cc1.1 ± 1.1 Cc14.4 ± 2.4 Bc37.8 ± 5.2 Ac61.7<0.01
Large larvae (without food)3.3 ± 1.7 Dbc13.3 ± 1.7 Cb35.6 ± 1.8 Bb80.0 ± 2.4 Aab54.9<0.01
F22.524.018.120.8
p<0.01<0.01<0.01<0.01
Table
Table 3. Mean short-term mortality (% ± SE) of Alphitobius diaperinus small and large larvae exposed to wheat treated with deltamethrin for 1 day, 3 days, 5 days, and 7 days. Within each row, means followed by the same uppercase letter are not significantly different (in all cases DF = 3, 35, Tukey-Kramer HSD test at p = 0.05). Within each column, means that are followed by the same lower-case letter are not significantly different (in all cases DF = 3, 35, Tukey-Kramer HSD test at p = 0.05). Where no letters exist, no significant differences were recorded.
Table 3. Mean short-term mortality (% ± SE) of Alphitobius diaperinus small and large larvae exposed to wheat treated with deltamethrin for 1 day, 3 days, 5 days, and 7 days. Within each row, means followed by the same uppercase letter are not significantly different (in all cases DF = 3, 35, Tukey-Kramer HSD test at p = 0.05). Within each column, means that are followed by the same lower-case letter are not significantly different (in all cases DF = 3, 35, Tukey-Kramer HSD test at p = 0.05). Where no letters exist, no significant differences were recorded.
Larval Group1 Day3 Days5 Days7 DaysFp
Small larvae (with food)46.7 ± 5.8 Ba98.9 ± 1.1 Aa100.0 ± 0.0 A100.0 ± 0.0 A20.6<0.01
Small larvae (without food)75.6 ± 5.3 Ba100.0 ± 0.0 Aa100.0 ± 0.0 A100.0 ± 0.0 A17.2<0.01
Large larvae (with food)1.1 ± 1.1 Bb62.2 ± 5.7 Ab98.9 ± 1.1 A98.9 ± 1.1 A224.7<0.01
Large larvae (without food)7.8 ± 3.6 Bb64.4 ± 5.0 Ab100.0 ± 0.0 A100.0 ± 0.0 A40.0<0.01
F41.421.81.01.0
p<0.01<0.010.410.41
Table
Table 4. Mean short-term mortality (% ± SE) of Alphitobius diaperinus small and large larvae exposed to wheat treated with piperonyl butoxide+acetamiprid+d-tetramethrin for 1 day, 3 days, 5 days, and 7 days. Within each row, means followed by the same uppercase letter are not significantly different (in all cases DF = 3, 35, Tukey-Kramer HSD test at p = 0.05). Within each column, means that are followed by the same lower-case letter are not significantly different (in all cases DF = 3, 35, Tukey-Kramer HSD test at p = 0.05).
Table 4. Mean short-term mortality (% ± SE) of Alphitobius diaperinus small and large larvae exposed to wheat treated with piperonyl butoxide+acetamiprid+d-tetramethrin for 1 day, 3 days, 5 days, and 7 days. Within each row, means followed by the same uppercase letter are not significantly different (in all cases DF = 3, 35, Tukey-Kramer HSD test at p = 0.05). Within each column, means that are followed by the same lower-case letter are not significantly different (in all cases DF = 3, 35, Tukey-Kramer HSD test at p = 0.05).
Larval Group1 Day3 Days5 Days7 DaysFp
Small larvae (with food)5.6 ± 2.4 Bb36.7 ± 5.3 Aa60.0 ± 7.6 Ab84.4 ± 5.6 Aa34.7<0.01
Small larvae (without food)12.2 ± 2.2 Ba58.9 ± 6.6 Aa100.0 ± 0.0 Aa100.0 ± 0.0 Aa41.4<0.01
Large larvae (with food)0.0 ± 0.0 Bc0.0 ± 0.0 Bc0.0 ± 0.0 Bd13.3 ± 3.3 Ab25.8<0.01
Large larvae (without food)0.0 ± 0.0 Cc11.1 ± 2.0 Bb37.8 ± 4.7 Ac67.8 ± 4.0 Aa131.2<0.01
F16.6103.9547.925.8
p<0.01<0.01<0.01<0.01
Table
Table 5. Mean long-term mortality (% ± SE) of Alphitobius diaperinus small and large larvae exposed for 7 days on untreated concrete dishes, with or without food, after 7 days of exposure on concrete dishes treated with etofenprox, deltamethrin and piperonyl butoxide+acetamiprid+d-tetramethrin. Within each row, asterisks indicate significant differences (two-tailed t-test at p = 0.05). Within each column, means that are followed by the same lower-case letter are not significantly different (Tukey-Kramer HSD test at p = 0.05). Where no letters or no asterisks exist, no significant differences were recorded. Where dashes exist, no analysis was performed.
Table 5. Mean long-term mortality (% ± SE) of Alphitobius diaperinus small and large larvae exposed for 7 days on untreated concrete dishes, with or without food, after 7 days of exposure on concrete dishes treated with etofenprox, deltamethrin and piperonyl butoxide+acetamiprid+d-tetramethrin. Within each row, asterisks indicate significant differences (two-tailed t-test at p = 0.05). Within each column, means that are followed by the same lower-case letter are not significantly different (Tukey-Kramer HSD test at p = 0.05). Where no letters or no asterisks exist, no significant differences were recorded. Where dashes exist, no analysis was performed.
InscecticideSmall LarvaeLarge Larvae
FoodNo FoodDFtpFoodNo FoodDFtp
Etofenprox64.1 ± 9.6100.0 ± 0.081.40.2024.0 ± 6.4 ab53.7 ± 12.0170.80.43
Deltamethrin-----0.0 ± 0.0 b----
Piperonyl butoxide+
acetamiprid+
d-tetramethrin		85.0 ± 9.6----33.5 ± 2.3 a68.2 ± 8.4 *174.2<0.01
DF12- 2, 1817
t−1.5- −1.4
F - 5.2
p0.15- 0.020.18
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Kavallieratos, N.G.; Nika, E.P.; Skourti, A.; Filintas, C.S.; Goumenou, T.D. Short- and Long-Term Mortalities of Small and Large Larvae of Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) on Concrete Surfaces Treated with Three Insecticides: Impact of Food. Insects 2022, 13, 366. https://0-doi-org.brum.beds.ac.uk/10.3390/insects13040366

AMA Style

Kavallieratos NG, Nika EP, Skourti A, Filintas CS, Goumenou TD. Short- and Long-Term Mortalities of Small and Large Larvae of Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) on Concrete Surfaces Treated with Three Insecticides: Impact of Food. Insects. 2022; 13(4):366. https://0-doi-org.brum.beds.ac.uk/10.3390/insects13040366

Chicago/Turabian Style

Kavallieratos, Nickolas G., Erifili P. Nika, Anna Skourti, Constantin S. Filintas, and Theofania D. Goumenou. 2022. "Short- and Long-Term Mortalities of Small and Large Larvae of Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) on Concrete Surfaces Treated with Three Insecticides: Impact of Food" Insects 13, no. 4: 366. https://0-doi-org.brum.beds.ac.uk/10.3390/insects13040366

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