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Article

Thermal Stability Changes in Telomeric G-Quadruplex Structures Due to N6-Methyladenine Modification

by 1 and 1,2,*
1
School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan
2
Graduate School of Bionics, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Ernesto Guccione
Received: 14 January 2021 / Revised: 26 January 2021 / Accepted: 27 January 2021 / Published: 2 February 2021
(This article belongs to the Special Issue Recent Advances in Biological Methylation)
N6-methyladenine modification (m6dA) has recently been identified in eukaryote genomic DNA. The methylation destabilizes the duplex structure when the adenine forms a Watson–Crick base pair, whereas the methylation on a terminal unpaired adenine stabilizes the duplex structure by increasing the stacking interaction. In this study, the effects of m6dA modification on the thermal stability of four distinct telomeric G-quadruplex (G4) structures were investigated. The m6dA-modified telomeric oligonucleotide d[AGGG(TTAGGG)3] that forms a basket-type G4 in Na+, d[(TTAGGG)4TT] that forms a hybrid-type G4 in K+ (Form-2), d[AAAGGG(TTAGGG)3AA] that forms a hybrid-type G4 in K+ (Form-1), and d[GGG(TTAGGG)3T] that forms a basket-type G4 with two G-tetrads in K+ (Form-3) were analyzed. Circular dichroism melting analysis demonstrated that (1) A7- and A19-methylation destabilized the basket-type G4 structure that formed in Na+, whereas A13-methylation stabilized the structure; (2) A15-methylation stabilized the Form-2 G4 structure; (3) A15- and A21-methylations stabilized the Form-1 G4 structure; and (4) A12-methylation stabilized the Form-3 G4 structure. These results suggest that m6dA modifications may affect the thermal stability of human telomeric G4 structures in regulating the biological functions. View Full-Text
Keywords: G-quadruplex; telomere; N6-methyladenine; thermal stability G-quadruplex; telomere; N6-methyladenine; thermal stability
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MDPI and ACS Style

Wada, R.; Yoshida, W. Thermal Stability Changes in Telomeric G-Quadruplex Structures Due to N6-Methyladenine Modification. Epigenomes 2021, 5, 5. https://0-doi-org.brum.beds.ac.uk/10.3390/epigenomes5010005

AMA Style

Wada R, Yoshida W. Thermal Stability Changes in Telomeric G-Quadruplex Structures Due to N6-Methyladenine Modification. Epigenomes. 2021; 5(1):5. https://0-doi-org.brum.beds.ac.uk/10.3390/epigenomes5010005

Chicago/Turabian Style

Wada, Ryohei, and Wataru Yoshida. 2021. "Thermal Stability Changes in Telomeric G-Quadruplex Structures Due to N6-Methyladenine Modification" Epigenomes 5, no. 1: 5. https://0-doi-org.brum.beds.ac.uk/10.3390/epigenomes5010005

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